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21.
Microenvironments and microbial community structure in sediments 总被引:3,自引:0,他引:3
The aim of this study was to explore the potential of a combined chemical and microbiological approach as part of a study of organic carbon oxidation processes in sediments. An assessment of microbiological diversity using molecular techniques was carried out in combination with high resolution chemical measurements at the sediment-water interface of a coastal lagoon affected by eutrophication in autumn 2000. There was a 0.2 mm overlap between the O2 and H2S profiles. pH showed a maximum just above the sediment-water interface coinciding with an oxygen maximum, suggesting photosynthetic activity, and a minimum coinciding with the O2-H2S interface. The redox potential was high in bottom water and surface sediment, reflecting the presence of oxygen and oxides, and reached low values after a step-wise decrease at -18 mm. Reduction of Fe occurred within the biofilm at the O2-H2S interface and was mostly due to reduction by H2S. The elevated concentrations of dissolved Mn in the oxic water may have been caused either by in situ production within organic aggregates or lateral water flow from sites nearby at which Mn2+ diffuses out of the sediment. Sequences related to sulphur chemolitotrophs were retrieved from the biofilm samples, which is consistent with the small overlap between O2 and H2S observed in this biofilm. Although the resolution of techniques used was different, sequencing results were consistent with chemical data in delineating the same horizons according to redox, pH or ecological properties. 相似文献
22.
Nitrogen uptake and transformation in a midwestern U.S. stream: A stable isotope enrichment study 总被引:1,自引:0,他引:1
Stephen K. Hamilton Jennifer L. Tank David F. Raikow Wilfred M. Wollheim Bruce J. Peterson Jackson R. Webster 《Biogeochemistry》2001,54(3):297-340
This study presents a comprehensive analysis ofnitrogen (N) cycling in a second-order forestedstream in southern Michigan that has moderatelyhigh concentrations of ammonium (mean,16 g N/L) and nitrate (17 g N/L). Awhole-stream 15NH4
+ addition wasperformed for 6 weeks in June and July, and thetracer 15N was measured downstream inammonium, nitrate, and detrital and livingbiomass. Ancillary measurements includedbiomass of organic matter, algae, bacteria andfungi, nutrient concentrations, hydrauliccharacteristics, whole-stream metabolism, andnutrient limitation assays. The resultsprovide insights into the heterotrophic natureof woodland streams and reveal the rates atwhich biological processes alter nitrogentransport through stream systems.Ammonium uptake lengths were 766–1349 m anduptake rates were 41–60 g N m–2min–1. Nitrate uptake could not bedetected. Nitrification rates were estimatedfrom the downstream increase in15N-enriched nitrate using a simulationmodel. The ammonium was removed bynitrification (57% of total uptake),heterotrophic bacteria and fungi associatedwith detritus (29%), and epilithic algae(14%). Growth of algae was likely limited bylight rather than nutrients, and dissolvedO2 revealed that the stream metabolism washeterotrophic overall (P:R = 0.2). Incubationsof detritus in darkened chambers showed thatuptake of 15N was mostly heterotrophic.Microbial N in detritus and algal N inepilithon appeared to reach isotopic steadystate with the dissolved ammonium, but theisotopic enrichment of the bulk detritus andepilithon did not approach that of ammonium,probably due to a large fraction of organic Nin the bulk samples that was not turning over. The actively cycling fraction of total N inorganic compartments was estimated from theisotopic enrichment, assuming uptake ofammonium but not nitrate, to be 23% forepilithon, 1% for fine benthic organic matter,5% for small woody debris, and 7% for leaves. These percentages agree with independentestimates of epilithic algal biomass, whichwere based on carbon:chlorophyll ratios in bulksamples and in algal fractions separated bydensity-gradient centrifugation in colloidalsilica, and of microbial N in the detritus,which were based on N released by chloroformfumigations. 相似文献
23.
Stability of the memory of eye position in a recurrent network of conductance-based model neurons 总被引:11,自引:0,他引:11
Studies of the neural correlates of short-term memory in a wide variety of brain areas have found that transient inputs can cause persistent changes in rates of action potential firing, through a mechanism that remains unknown. In a premotor area that is responsible for holding the eyes still during fixation, persistent neural firing encodes the angular position of the eyes in a characteristic manner: below a threshold position the neuron is silent, and above it the firing rate is linearly related to position. Both the threshold and linear slope vary from neuron to neuron. We have reproduced this behavior in a biophysically plausible network model. Persistence depends on precise tuning of the strength of synaptic feedback, and a relatively long synaptic time constant improves the robustness to mistuning. 相似文献
24.
Srivastava BK Soni R Patel JZ Solanki M Valani D Gupta S Mishra B Takale V Pandya P Jain MR Patel PR 《Bioorganic & medicinal chemistry letters》2007,17(18):5227-5232
Design and synthesis of a few novel methylamino piperidinyl substituted oxazolidinones are reported. Their antibacterial activities have been evaluated in a MIC assay against broader panel of both susceptible and resistant Gram-positive strains. (S)-N-{3-[3-Fluoro-4-(methyl-{1-[3-(5-nitrofuran-2-yl)-acryloyl]-piperidin-4-yl}-amino)-phenyl]-2-oxo-oxazolidin-5-ylmethyl}-acetamide 4i has shown comparable antibacterial activity to linezolid and eperezolid in the MIC assay, additionally compound 4i showed good antibacterial activity with an in vitro MIC value of 2-4 microg/mL against linezolid resistant Staphylococcus aureus (linezolid 16 microg/mL). 相似文献
25.
Sethna Lienne R. Royer Todd V. Speir Shannon L. Trentman Matt T. Mahl Ursula H. Hagemeier Leah P. Tank Jennifer L. 《Biogeochemistry》2022,159(2):265-282
Biogeochemistry - Agriculture alters the biogeochemical cycling of nutrients such as nitrogen (N), phosphorus (P), and silicon (Si) which contributes to the stoichiometric imbalance among these... 相似文献
26.
Scott D. Tiegs Peter S. Levi Janine Rüegg Dominic T. Chaloner Jennifer L. Tank Gary A. Lamberti 《Ecosystems》2011,14(4):598-614
We tested the hypothesis that the carcasses of anadromous Pacific salmon (Oncorhynchus spp.) constitute a significant source of nutrients in the nutrient-poor freshwaters where these fish migrate, spawn, senesce,
and die. In a 110 m-long stream reach in Southeast Alaska, we retained nearly 3000 salmon carcasses and compared streamwater
nitrogen (N), phosphorus (P), and the biomass of benthic biofilm in this reach with an upstream reference reach. The study
spanned 5 months, bracketed the entire salmon run, and encompassed significant seasonal variation in abiotic stream conditions.
Concentrations of dissolved and particulate N and P followed distinctly unimodal patterns through time, which tracked the
abundance of live salmon, and we observed strong predictive relationships between live-salmon abundance and streamwater-nutrient
concentrations. In contrast, we did not observe clear relationships between salmon carcasses and streamwater nutrients. Biofilm
biomass within our study reaches seemed to more closely track the abundance of live salmon than the abundance of carcasses.
The experimental retention of carcasses had a minor or undetectable influence on nutrient concentrations and biofilm within
the study reach as compared to the reference reach. We conclude that physical factors such as temperature, discharge, nutrient
limitation, and irradiance vary seasonally in ways that maximize the influence of nutrients provisioned by live salmon and
minimize the influence of carcass-derived nutrients on the aspects of stream ecosystems that we examined. Overall, our results
promote a new perspective on the ecological role of salmon in freshwaters, and contribute to a more mechanistic understanding
of how migratory fishes can influence aquatic ecosystems. 相似文献
27.
Kaphalia L Kaphalia BS Kumar S Kanz MF Treinen-Moslen M 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2006,830(2):231-237
A rapid and sensitive high-performance liquid chromatographic method was developed for determination of diclofenac and its major metabolite, 4'-hydroxydiclofenac, in serum from rats treated with diclofenac. The method is simple with a one-step extraction procedure, isocratic HPLC separation, and UV detection at 280 nm. Use of N-phenylanthranilic acid as the internal standard provided good accuracy without interference by endogenous compounds or 5-hydroxydiclofenac, another metabolite of interest. Limits of detection for diclofenac and 4'-hydroxydiclofenac were 0.0225 and 0.0112 microg/ml, respectively. Average extraction efficiencies of diclofenac, 4'-hydroxydiclofenac, and the internal standard were >/=76%. The method was applied to serum collected at 3h after rats were treated with an experimentally useful dosage range of 3, 10 and 50mg/kg diclofenac. Recovery (as a percentage of dose) for the 4'-hydroxy metabolite in serum was found to consistently average from 0.10 to 0.12% following each dosage, whereas recovery of diclofenac in serum declined from 0.45 to 0.37%. Thus, the method is suitable for measurement of a major diclofenac metabolite in experimental studies. 相似文献
28.
Timothy J. Hoellein Jennifer L. Tank John J. Kelly Emma J. Rosi-Marshall 《Hydrobiologia》2010,649(1):331-345
Humans have increased the availability of nutrients including nitrogen and phosphorus worldwide; therefore, understanding
how microbes process nutrients is critical for environmental conservation. We examined nutrient limitation of biofilms colonizing
inorganic (fritted glass) and organic (cellulose sponge) substrata in spring, summer, and autumn in three streams in Michigan,
USA. Biofilms were enriched with nitrate (NO3
−), phosphate (PO4
3−), ammonium (NH4
+), NO3
− + PO4
3−, NH4
+ + PO4
3−, or none (control). We quantified biofilm structure and function as chlorophyll a (i.e., primary producer biomass) and community respiration on all substrata. In one stream, we characterized bacterial and
fungal communities on cellulose in autumn using clone library sequencing and denaturing gradient gel electrophoresis to determine
if community structure was linked to nutrient limitation status. Despite oligotrophic conditions, primary producer biomass
was infrequently nutrient limited. In contrast, respiration on organic substrata was frequently limited by N + P combinations.
We found no difference between biofilm response to NH4
+ versus NO3
− enrichment, although the response to both N-species was positively related to water column PO4
3− concentrations and temperature. Molecular analysis for fungal community composition suggested no relationship to nutrient
limitation, but the dominant members of the bacterial community on cellulose were different on NO3
−, PO43, and NO3
− + PO4
3− treatments relative to control, NH4
+, and NH4
+ + PO4
3− treatments, which matched patterns for biofilm respiration rates from each treatment. Our results show discrete patterns
of nutrient limitation dependent upon substratum type and season, and imply changes in bacterial community structure and function
may be linked following nutrient enrichment in streams. 相似文献
29.
We report that the ability to absorb water vapor from the air in larvae of the American dog tick, Dermacentor variabilis, changes depending upon moisture conditions where the eggs develop. When development occurs at lower relative humidities, resultant larvae can replenish water stores, maintain water balance, and survive at relative humidities as low as 75-85% RH, a range that agrees with previously published values for the critical equilibrium humidity or CEH. In contrast, exposure to high relative humidity conditions during development elevates the CEH to 93-97% RH. These larvae can survive only at relative humidities that are close to saturation, as 93% RH is a dehydrating atmosphere. For these larvae, absorption at 97% RH can be prevented by blocking the mouthparts with wax, indicating that an upward shift has occurred in the moisture threshold where the active mechanism for water vapor absorption operates. Based on transfer experiments between low and high relative humidities, the CEH of larvae is determined by the relative humidity experienced by the mother rather than the moisture conditions encountered by eggs after they are laid. The fact that no changes in body water content, dehydration tolerance limit and water loss rate were observed implies that adjustments to the CEH conferred by the mother have the adaptive significance of enabling larvae to maintain water balance by limiting the range of hydrating atmospheres. 相似文献
30.
Mahajan K Coppola D Rawal B Chen YA Lawrence HR Engelman RW Lawrence NJ Mahajan NP 《The Journal of biological chemistry》2012,287(26):22112-22122
Androgen deprivation therapy has been the standard of care in prostate cancer due to its effectiveness in initial stages. However, the disease recurs, and this recurrent cancer is referred to as castration-resistant prostate cancer (CRPC). Radiotherapy is the treatment of choice; however, in addition to androgen independence, CRPC is often resistant to radiotherapy, making radioresistant CRPC an incurable disease. The molecular mechanisms by which CRPC cells acquire radioresistance are unclear. Androgen receptor (AR)-tyrosine 267 phosphorylation by Ack1 tyrosine kinase (also known as TNK2) has emerged as an important mechanism of CRPC growth. Here, we demonstrate that pTyr(267)-AR is recruited to the ATM (ataxia telangiectasia mutated) enhancer in an Ack1-dependent manner to up-regulate ATM expression. Mice engineered to express activated Ack1 exhibited a significant increase in pTyr(267)-AR and ATM levels. Furthermore, primary human CRPCs with up-regulated activated Ack1 and pTyr(267)-AR also exhibited significant increase in ATM expression. The Ack1 inhibitor AIM-100 not only inhibited Ack1 activity but also was able to suppress AR Tyr(267) phosphorylation and its recruitment to the ATM enhancer. Notably, AIM-100 suppressed Ack1 mediated ATM expression and mitigated the growth of radioresistant CRPC tumors. Thus, our study uncovers a previously unknown mechanism of radioresistance in CRPC, which can be therapeutically reversed by a new synergistic approach that includes radiotherapy along with the suppression of Ack1/AR/ATM signaling by the Ack1 inhibitor, AIM-100. 相似文献