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191.
Subhajit Dinda Sujoy RoyChowdhury K.M. Abdul Malik Ramgopal Bhattacharyya 《Inorganica chimica acta》2009,362(7):2108-2116
Thiocyanate ions reduce perrhenate in aqueous acidic solution, and on addition of a suitable countercation (PPh4Cl) afford (PPh4)2[Re(NCS)6] (1) and (PPh4)2[ReO(NCS)5] (2), which have been confirmed by X-ray crystallography. The kinetics of the above reaction has been studied. Both the complexes exhibit efficient and highly selective catalytic epoxidation ability in the presence of NaHCO3 as a co-catalyst and competent catalytic properties in the selective oxidation of alcohols to the corresponding aldehydes or ketones in the presence of pyrazole as an additive and sulfides to sulfoxides and sulfones. H2O2 was used as the terminal oxidant in all the above-mentioned oxidation reactions. 相似文献
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193.
Chakraborty N Bhattacharyya S De C Mukherjee A Sarkar RN Banerjee D Chakraborti S Bhattacharyya SK 《Journal of microbiological methods》2009,76(1):12-17
As tuberculosis generates a highly heterogeneous antibody repertoire, its diagnosis requires tests based on cocktails of antigens. We describe a new, rapid method called rapid immunochromatographic assay (RICA) for cocktail-based diagnosis, which can detect Mycobacterial antigens in sputum specimens. Six antigenic fractions of pathogenic Mycobacterium tuberculosis were used in combination as the capture antigens in the control line of the flow-through assay. Antigen detection of 200 sputum samples from HIV seropositive patients by RICA assay gave a sensitivity of 97.9%, specificity of 99.0%, positive predictive value of 98.9%, negative predictive value of 98.0%, false positive rate of 0.9%, false negative rate of 2.0%, prevalence rate of 49%, likelihood ratio for positive results 97 and likelihood ratio for negative results 0.02. The combination of RICA and AFB staining gave a sensitivity of 100%, specificity of 100%, positive predictive value of 100%, negative predictive value of 100%, false positive rate of 0%, false negative rate of 0%, likelihood ratio for negative results 0. The assay was simple, rapid and economical for the detection of M. tuberculosis infection and suitable for large scale screening of samples in endemic areas without any sophisticated equipment. The results of the assay proved to be superior to conventional methods and combined with clinical data, could form the basis for starting an earlier course of treatment. 相似文献
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195.
Andrea Salis Mani Shankar Bhattacharyya Maura Monduzzi Vincenzo Solinas 《Journal of Molecular Catalysis .B, Enzymatic》2009,57(1-4):262-269
The present work investigates the influence of the support surface on the loading and the enzymatic activity of the immobilized Pseudomonas fluorescens lipase. Different porous materials, polypropylene (Accurel), polymethacrylate (Sepabeads EC-EP), silica (SBA-15 and surface modified SBA-15), and an organosilicate (MSE), were used as supports. The immobilized biocatalysts were compared towards sunflower oil ethanolysis for the sustainable production of biodiesel. Since the supports have very different structural (ordered hexagonal and disordered) and textural features (surface area, pore size, and total pore volume), in order to consider only the effect of the support surface, experiments were performed at low surface coverage. The different functional groups occurring on the support surface allowed either physical (Accurel, MSE, and SBA-15) or chemical adsorption (Sepabeads EC-EP and SBA-15–R-CHO). The surface-modified SBA-15 (SBA-15–R-CHO) allowed the highest loading. The lipase immobilized on the MSE was the most active biocatalyst. However, in terms of catalytic efficiency (activity/loading) the lipase immobilized on the SBA-15, the support that allowed the lowest loading, was the most efficient. 相似文献
196.
197.
Reich S Puckey LH Cheetham CL Harris R Ali AA Bhattacharyya U Maclagan K Powell KA Prodromou C Pearl LH Driscoll PC Savva R 《Protein science : a publication of the Protein Society》2006,15(10):2356-2365
Exploitation of potential new targets for drug and vaccine development has an absolute requirement for multimilligram quantities of soluble protein. While recombinant expression of full-length proteins is frequently problematic, high-yield soluble expression of functional subconstructs is an effective alternative, so long as appropriate termini can be identified. Bioinformatics localizes domains, but doesn't predict boundaries with sufficient accuracy, so that subconstructs are typically found by trial and error. Combinatorial Domain Hunting (CDH) is a technology for discovering soluble, highly expressed constructs of target proteins. CDH combines unbiased, finely sampled gene-fragment libraries, with a screening protocol that provides "holistic" readout of solubility and yield for thousands of protein fragments. CDH is free of the "passenger solubilization" and out-of-frame translational start artifacts of fusion-protein systems, and hits are ready for scale-up expression. As a proof of principle, we applied CDH to p85alpha, successfully identifying soluble and highly expressed constructs encapsulating all the known globular domains, and immediately suitable for downstream applications. 相似文献
198.
Bhattacharyya A Thakur AK Chellgren VM Thiagarajan G Williams AD Chellgren BW Creamer TP Wetzel R 《Journal of molecular biology》2006,355(3):524-535
There are nine known expanded CAG repeat neurological diseases, including Huntington's disease (HD), each involving the repeat expansion of polyglutamine (polyGln) in a different protein. Similar conditions can be induced in animal models by expression of the polyGln sequence alone or in other protein contexts. Besides the polyGln sequence, the cellular context of the disease protein, and the sequence context of the polyGln within the disease protein, are both likely to contribute to polyGln physical behavior and to pathology. In HD, the N-terminal, exon-1 segment of the protein huntingtin contains the polyGln sequence immediately followed by an oligoproline region. We show here that introduction of a P10 sequence C-terminal to polyGln in synthetic peptides decreases both the rate of formation and the apparent stability of the amyloid-like aggregates associated with this family of diseases. The sequence can be trimmed to P6 without altering the suppression, but a P3 sequence is ineffective. Spacers up to at least three amino acid residues in length can be inserted between polyGln and P10 without altering this effect. There is no suppression, however, when the P10 sequence is either placed on the N-terminal side of polyGln or attached to polyGln via a side-chain tether. The nucleation mechanism of a Q40 sequence is unchanged upon addition of a P10 C-terminal extension, yielding a critical nucleus of one. The effects of oligoPro length and structural context on polyGln aggregation are correlated strongly with alterations in the circular dichroism spectra of the monomeric peptides. For example, the P10 sequence eliminates the small amount of alpha helical content otherwise exhibited by the Q40 sequence. The P10 sequence may suppress aggregation by stabilizing an aggregation-incompetent conformation of the monomer. The effect is transportable: a P10 sequence fixed to the C terminus of the sequence Abeta similarly modulates amyloid fibril formation. 相似文献
199.
200.
Bhattacharyya N Pechhold K Shahjee H Zappala G Elbi C Raaka B Wiench M Hong J Rechler MM 《The Journal of biological chemistry》2006,281(34):24588-24601
Insulin-like growth factor binding protein-3 (IGFBP-3), a secreted protein, has the intrinsic ability to induce apoptosis directly without binding insulin-like growth factors. Previous studies suggested that IGFBP-3 must be secreted to exert its biological functions. IGFBP-3 contains a nuclear localization signal (NLS), and exogenous IGFBP-3 is translocated into the nucleus, suggesting that both secretion and nuclear localization may play important roles in IGFBP-3 action. To address these questions, we fused yellow fluorescent protein (YFP) to mature IGFBP-3 lacking its signal peptide so that it would remain intracellular and mutated the C-terminal NLS of IGFBP-3, (228)KGRKR(232), to MDGEA. Following transfection of PC-3 human prostate cancer cells with these constructs, Western blots indicated that YFP-IGFBP-3 lacking a signal peptide was cell-associated and not present in the extracellular media. Moreover, the fusion protein was not N-glycosylated, indicating that it had not entered the secretory pathway. Confocal imaging showed that intracellular YFP-MDGEA-IGFBP-3 was predominantly cytoplasmic. Transient transfection of nonsecreted YFP-wild-type IGFBP-3 decreased cell viability, as assessed by staining with annexin V followed by flow cytometry. Induction of cell death was caspase-dependent, indicative of apoptosis. Apoptosis also was induced by the nonsecreted NLS mutant (YFP-MDGEA-IGFBP-3) alone and when the IGF-binding site also had been mutated. These results indicate that IGFBP-3 can induce apoptosis in an IGF-independent manner without being secreted or concentrated in the nucleus. 相似文献