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991.
K K Kohli S C Bhatia T A Venkitasubramanian 《Environmental physiology & biochemistry》1975,5(2):119-125
The effect of a single oral dose of dieldrin (30 mg/kg body weight) on lipid metabolism in rats was studied. Liver lipids content increased and this increase was mainly in the triglyceride fraction. The incorporation of acetate-14C into fatty acids was decreased indicating an inhibition of lipogenesis. Fatty acid oxidation was increased. Palmitate-14C incorporation into the triglyceride fraction was enhanced pointing to an overall increased utilization of fatty acids. 相似文献
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Veneta Gerganova Payal Bhatia Vincent Vincenzetti Sophie G. Martin 《Molecular biology of the cell》2021,32(8):703
The fission yeast cells Schizosaccharomyces pombe divide at constant cell size regulated by environmental stimuli. An important pathway of cell size control involves the membrane-associated DYRK-family kinase Pom1, which forms decreasing concentration gradients from cell poles and inhibits mitotic inducers at midcell. Here, we identify the phosphatase 2C Ptc1 as negative regulator of Pom1. Ptc1 localizes to cell poles in a manner dependent on polarity and cell-wall integrity factors. We show that Ptc1 directly binds Pom1 and can dephosphorylate it in vitro but modulates Pom1 localization indirectly upon growth in low-glucose conditions by influencing microtubule stability. Thus, Ptc1 phosphatase plays both direct and indirect roles in the Pom1 cell size control pathway. 相似文献
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Development of chickpea EST-SSR markers and analysis of allelic variation across related species 总被引:1,自引:0,他引:1
Shalu Choudhary Niroj Kumar Sethy Bhumika Shokeen Sabhyata Bhatia 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(3):591-608
Despite chickpea being the third important grain legume, there is a limited availability of genomic resources, especially
of the expressed sequence tag (EST)-based markers. In this study, we generated 822 chickpea ESTs from immature seeds as well
as exploited 1,309 ESTs from the chickpea database, thus utilizing a total of 2,131 EST sequences for development of functional
EST-SSR markers. Two hundred and forty-six simple sequence repeat (SSR) motifs were identified from which 183 primer pairs
were designed and 60 validated as functional markers. Genetic diversity analysis across 30 chickpea accessions revealed ten
markers to be polymorphic producing a total of 29 alleles and an observed heterozygosity average of 0.16 thereby exhibiting
low levels of intra-specific polymorphism. However, the markers exhibited high cross-species transferability ranging from
68.3 to 96.6% across the six annual Cicer species and from 29.4 to 61.7% across the seven legume genera. Sequence analysis of size variant amplicons from various species
revealed that size polymorphism was due to multiple events such as copy number variation, point mutations and insertions/deletions
in the microsatellite repeat as well as in the flanking regions. Interestingly, a wide prevalence of crossability-group-specific
sequence variations were observed among Cicer species that were phylogenetically informative. The neighbor joining dendrogram clearly separated the chickpea cultivars
from the wild Cicer and validated the proximity of C. judaicum with C. pinnatifidum. Hence, this study for the first time provides an insight into the distribution of SSRs in the chickpea transcribed regions
and also demonstrates the development and utilization of genic-SSRs. In addition to proving their suitability for genetic
diversity analysis, their high rates of transferability also proved their potential for comparative genomic studies and for
following gene introgressions and evolution in wild species, which constitute the valuable secondary genepool in chickpea.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
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