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541.
Tikka Chiranjeevi Osuru Hari Prasad Uppu Venkateswara Prasad Avula Kishor Kumar Veeraraghavulu Praveen Chakravarthi Paramala Balaji Rao Potuguchi Venkata Gurunadha Krishna Sarma Nagi reddy Raveendra Reddy Matcha Bhaskar 《Bioinformation》2014,10(4):241-245
Periodontitis have been referred to as the sixth complication of diabetes found in high prevalence among diabetic patients than
among healthy controls. The aim of the present study was to examine the periodontal disease status among collected dental plaque
samples. Chromosomal DNA was isolated and amplified by universal primers. The DNA was sequenced for bacterial confirmation
and phylogenetic analysis performed for the evolutionary relationship with other known pathogens. No amplification products
were observed in groups labeled non periodontal and non Diabetes (NP&ND) and non Periodontal and Diabetes (NP&D). But in
the case of Periodontal and non Diabetes (P&ND) groups 22 amplified products were observed. In case of Periodontal and Diabetes
(P&D), 32 amplified products were positive for microbes. Among the four microbial groups, Treponemadenticola, and Tannerella
forsythia were found to be prevalent in P&D. The phylogenetic analysis of 16s rRNA of Treponemadenticola showed the relationship
with other Treponema oral pathogen species and with the Spirochaetazuelaera. Tannerella forsythia shows its evolutionary relationship
only with four oral pathogens (Macellibacteroidesfermentans, Porphyromadaceae bacterium, Parabacteroidesmeredae and Bacillus fosythus).
Prevotellaintermedia also showed its evolutionary relationship only with Prevotella Spcs while Fusobacterium revealed close
evolutionary relationship only with Porpiromonasgingivalis. 相似文献
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Padua Rodolfo A.; Baron Kyle T.; Thyagarajan Bhaskar; Campbell Colin; Thayer Stanley A. 《American journal of physiology. Cell physiology》1998,274(3):C615
Physiological and pathologicalCa2+ loads are thought to be takenup by mitochondria via a process dependent on aerobic metabolism. Wesought to determine whether human diploid fibroblasts from a patientwith an inherited defect in pyruvate dehydrogenase (PDH) exhibit adecreased ability to sequester cytosolicCa2+ into mitochondria.Mobilization of Ca2+ stores withbradykinin (BK) increased the cytosolicCa2+ concentration([Ca2+]c)to comparable levels in control and PDH-deficient fibroblasts. Innormal fibroblasts transfected with plasmid DNA encodingmitochondrion-targeted apoaequorin, BK elicited an increase inCa2+-dependent aequorinluminescence corresponding to an increase in the mitochondrialCa2+ concentration([Ca2+]mt)of 2.0 ± 0.2 µM. The mitochondrial uncoupling agent carbonyl cyanidep-(trifluoromethoxy)phenylhydrazoneblocked the BK-induced [Ca2+]mtincrease, although it did not affect the[Ca2+]ctransient. Basal[Ca2+]cand[Ca2+]mtin control and PDH-deficient cells were similar. However, confocalimaging of the potential-sensitive dye JC-1 indicated that thepercentage of highly polarized mitochondria was reduced from 30 ± 1% in normal cells to 19 ± 2% in the PDH-deficient fibroblasts. BK-elicited[Ca2+]mttransients in PDH-deficient cells were reduced to 4% of control, indicating that PDH-deficient mitochondria have a decreased ability totake up cytosolic Ca2+. Thus cellswith compromised aerobic metabolism have a reduced capacity tosequester Ca2+. 相似文献
545.
A method based on Rimini test for aliphatic amines was studied and developed for quantitative estimation of aliphatic primary
amines. The method involves action of the amine with acetone to form schiff base which complexes with sodium nitroprusside
to give violet colour. The absorption maximum in the visible range of the spectrum, for the reaction mixture was found to
be 550 nm. The pH (8–11) and reaction time scan for the assay were optimized. A linear relation of concentration (0.2–3 mg/mL)
of amine against absorbance at 550 nm was established. Interference due to other reaction components was negligible (±0.02 mg/mL)
as compared to the sample in buffer. 1, 3-dimethyl butylamine was used as the model amine and the method was applied to other
amines; it was observed that when electron-withdrawing substituents are present in the molecule the reaction is retarded,
as the incubation time was longer. This method is useful for estimation of aliphatic primary amine in biological samples. 相似文献
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Background
For a proper understanding of protein structure and folding it is important to know if a polypeptide segment adopts a conformation inherent in the sequence or it depends on the context of its flanking secondary structures. Turns of various lengths have been studied and characterized starting from three-residue γ-turn to six-residue π-turn. The Schellman motif occurring at the C-terminal end of α-helices is a classical example of hydrogen bonded π-turn involving residues at (i) and (i+5) positions. Hydrogen bonded and non-hydrogen bonded β- and α-turns have been identified previously; likewise, a systematic characterization of π-turns would provide valuable insight into turn structures. 相似文献548.
Amit Kumar Rai H.C. Swapna N. Bhaskar P.M. Halami N.M. Sachindra 《Enzyme and microbial technology》2010,46(1):9-13
Fish viscera are an important source for biomolecules such as protein and lipids. Studies were carried out to assess fermentation ensilaging as a method for recovery of oil from fresh water fish viscera. The total lipid content in the viscera ranged from 19% to 21% and upto 85% of this could be recovered by fermentation. Fermentation using added lactic cultures (Enterococcus faecium HAB01 and Pediococcus acidilactici K7) did not show any advantage over natural fermentation with respect to recovery of oil and no differences were observed in fatty acid composition of oil recovered by fermentation using different cultures. Activity of acidic, neutral and alkaline proteases decreased during fermentation. Eventhough degree of protein hydrolysis increased during fermentation with highest (62.3%) being in fermentation using Pediococcus acidilactici K7 no differences were found in oil recovery. With decrease in protease activity the rate of change in degree of hydrolysis also decreased. 相似文献
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