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411.
Phosphorylation of secreted forms of human beta 2-interferon/hepatocyte stimulating factor/interleukin-6 总被引:3,自引:0,他引:3
L T May U Santhanam S B Tatter N Bhardwaj J Ghrayeb P B Sehgal 《Biochemical and biophysical research communications》1988,152(3):1144-1150
"Beta 2-Interferon/hepatocyte stimulating factor/interleukin-6" (IFN-beta 2) has emerged as a major mediator of the plasma protein response to tissue injury (the acute phase response) in addition to its numerous effects on cells of the immune system. Human fibroblasts and monocytes induced with tumor necrosis factor, interleukin-1, bacterial lipopolysaccharide (endotoxin) or virus infection secrete multiple forms of differentially glycosylated IFN-beta 2 polypeptides: at least a doublet of molecular mass approximately 25 kD and a triplet of mass approximately 30 kD. We report that immunoprecipitation analyses of medium from [32P]orthophosphate- labeled cultures of induced fibroblasts carried out using a rabbit polyclonal antibody to recombinant E. coli-derived human IFN-beta 2 reveal that the secreted gp23-25 and gp28-30 forms of IFN-beta 2 are phosphorylated. IFN-beta 2 gp23-25 secreted by induced monocytes is phosphorylated whereas the monocytic gp28-30 is poorly labeled with [32P]orthophosphate suggesting tissue-specific differences in IFN-beta 2 phosphorylation. Phosphoamino acid analyses indicate that all of the detected phosphate is in phosphoserine residues. Furthermore, IFN-beta 2 can be completely dephosphorylated by alkaline phosphatase (E.C. No. 3.1.3.1); thus all of the phosphate label is in readily accessible sites. These observations suggest the possibility that differential phosphorylation of IFN-beta 2 forms may be a mechanism to modulate its functions in a tissue-specific manner. 相似文献
412.
Richard H. Zimmerman S. V. Bhardwaj Ingrid M. Fordham 《Plant Cell, Tissue and Organ Culture》1995,43(3):207-213
Six cultivars of apple and two of red raspberry consistently produced equal or significantly better shoot proliferation on modified Murashige and Skoog medium gelled with a mixture of corn starch and Gelrite than on the same medium gelled with agar. Two pear cultivars grown on starch-Gelrite medium produced hyperhydric shoots and almost no growth, but the addition of a polysaccharide hydric control (antivitrifying) agent to the medium eliminated hyperhydricity. The resulting shoot proliferation equaled or exceeded that on the agar-gelled medium. The starch-Gelrite mixture is easy to prepare and gelling agent costs are only 10–15% of agar, or less if starch is purchased in bulk. Although the opaque gray-white medium makes it more difficult to detect internal contaminants, external contaminants are easily discerned.The US Government's right to retain a non-exclusive royalty-free license on and to any copyright is acknowledged. 相似文献
413.
Using variable to maximum fluorescence (Fv/Fm) as the criterion, the down regulation of photosynthesis by high light stress was characterized in the detached leaves of Artabotrys hexapetatus. The decrease in Fv/Fm was corelated with the decrease in oxygen evolution by thylakoids isolated from high light exposed leaves. The decrease in Fv/Fm was linear with increasing time of exposure to high light. A comparison of recovery measured as Fv/Fm, in low light versus dark, revealed that the recovery in darkness was as significant as in low light. Since the relaxation of fluorescence was a rapid response after exposure to high light and the fact that the recovery occurs in total darkness, it is concluded that photoinhibition and down regulation of photosynthesis by high light are independent events.Abbreviation Fpl-
initial plateau
- Fm-
maximum fluorescence
- Fo-
prompt fluorescence
- Fv-
variable fluorescence
- PFD-
photon flux density
- PS I (II)-
Photosystem I (II) 相似文献
414.
Effect of trypsin treatment of photosystem I particles on the electron donation to p700 总被引:1,自引:0,他引:1
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Proteolysis of photosystem I particles had no effect on P700 oxidation but did inhibit the rate of P700+ reduction. The Vmax values were decreased for both dichlorophenol and plastocyanin, but the Km values were unaffected indicating that trypsin treatment altered electron transfer rather than the binding of the donor to the photosystem I complex. The salt dependence of P700+ reduction was unaffected. The effects of P700+ reduction were the same for the preparations of different workers (Shiozawa, Alberte, Thornber 1974 Arch Biochem Biophys 165: 388; and Bengis, Nelson 1975 J Biol Chem 250: 2783).
In both cases, the 70-kilodalton, chlorophyll-containing polypeptide was digested confirming its role in transferring electrons from plastocyanin to P700. The fact that the preparation of Shiozawa et al. lacks subunit (III) but still used plastocyanin as the electron donor rules out a role for this subunit as “the plastocyanin binding protein.” Subunit III was also digested in the Bengis and Nelson preparation.
相似文献415.
416.
Neutralizing Monoclonal Antibodies Block Human Immunodeficiency Virus Type 1 Infection of Dendritic Cells and Transmission to T Cells 总被引:8,自引:2,他引:6
Sarah S. Frankel Ralph M. Steinman Nelson L. Michael Silvia Ratto Kim Nina Bhardwaj Melissa Pope Mark K. Louder Philip K. Ehrenberg Paul W. H. I. Parren Dennis R. Burton Hermann Katinger Thomas C. VanCott Merlin L. Robb Deborah L. Birx John R. Mascola 《Journal of virology》1998,72(12):9788-9794
Prevention of the initial infection of mucosal dendritic cells (DC) and interruption of the subsequent transmission of HIV-1 from DC to T cells are likely to be important attributes of an effective human immunodeficiency virus type 1 (HIV-1) vaccine. While anti-HIV-1 neutralizing antibodies have been difficult to elicit by immunization, there are several human monoclonal antibodies (MAbs) that effectively neutralize virus infection of activated T cells. We investigated the ability of three well-characterized neutralizing MAbs (IgG1b12, 2F5, and 2G12) to block HIV-1 infection of human DC. DC were generated from CD14+ blood cells or obtained from cadaveric human skin. The MAbs prevented viral entry into purified DC and the ensuing productive infection in DC/T-cell cultures. When DC were first pulsed with HIV-1, MAbs blocked the subsequent transmission to unstimulated CD3+ T cells. Thus, neutralizing antibodies can block HIV-1 infection of DC and the cell-to-cell transmission of virus from infected DC to T cells. These data suggest that neutralizing antibodies could interrupt the initial events associated with mucosal transmission and regional spread of HIV-1. 相似文献
417.
Jitender K. Bhardwaj Prerna Bikal Som Nath Sachdeva 《Journal of biochemical and molecular toxicology》2023,37(7):e23371
Increase in success of cancer treatment with advancement in the screening, prognosis and diagnosis protocols have significantly improved the rate of cancer survivorship. With the declining cancer mortality, however, the cancer survivors are also subjected to the adverse consequences of chemotherapy, particularly in the female reproductive system. Recent studies have shown the sensitivity of the ovarian tissue to the chemotherapeutic drugs-induced toxicity. Several in vitro and in vivo studies have assessed the toxic effects of chemotherapeutic drugs. The most frequently used chemotherapeutic drugs such as doxorubicin, cyclophosphamide, cisplatin and paclitaxel have been reported to cause ovarian damage, diminution of follicular pool reserve, premature ovarian failure and early menopause, resulting into declining fertility potential among females. The chemotherapy often employs combination of drug regimen to increase the efficacy of the treatment. However, the literature mostly consists of clinical data regarding the gonadotoxicity caused by anticancer drugs but there lacks the understanding of toxicity mechanism. Therefore, understanding of the different toxicity mechanisms will be helpful in development of possible therapeutic interventions for preservation of declining female fertility among cancer survivors. The current review comprehends the underlying mechanisms of female reproductive toxicity induced by the most commonly used chemotherapeutic drugs. In addition, the review also summarizes the recent findings related to the use of various protectants to diminish or at least in managing the toxicity induced by different chemotherapeutic drugs in females. 相似文献
418.
Human red and green visual pigment genes are X-linked duplicate genes. To
study their evolutionary history, introns 2 and 4 (1,987 and 1,552 bp,
respectively) of human red and green pigment genes were sequenced.
Surprisingly, we found that intron 4 sequences of these two genes are
identical and that the intron 2 sequences differ by only 0.3%. The low
divergences are unexpected because the duplication event producing the two
genes is believed to have occurred before the separation of the human and
Old World monkey (OWM) lineages. Indeed, the divergences in the two introns
are significantly lower than both the synonymous divergence (3.2% +/- 1.1%)
and the nonsynonymous divergence (2.0% +/- 0.5%) in the coding sequences
(exons 1-6). A comparison of partial sequences of exons 4 and 5 of human
and OWM red and green pigment genes supports the hypothesis that the gene
duplication occurred before the human-OWM split. In conclusion, the high
similarities in the two intron sequences might be due to very recent gene
conversion, probably during evolution of the human lineage.
相似文献
419.
Efficient oxygenic photosynthesis not only requires synchronous turover and operation of photosystem I (PS I) and photosystem
II (PS II) but also the preferential turnover of PS I for cyclic photophosphorylation to maintain required ATP and NADPH ratio
during carbon dioxide reduction. Ohe initial higher rate of turnover of PS IIin viva is accounted by the fact that (i) PS I contains only about one-third of total chlorophylls, (ii) about 90% of light harvesting
a/b protein (LAC) which accounts for about 50% of the total chlorophylls, remains associated with PS II as PS II-LHC II complexes
(PS IIα and (iii) the ratio of PS II/PS I is always greater than unity, in the range of 1–2 : 1 under different environmental regimes.
Ohe initial preferential feeding of PS II, due to its larger antenna, is bound to result in faster rate of turn over of PS
II than PS I, leading to higher rate of reduction of an intersystem carrier than the rate of its oxidation by PS I. Ohe light
dependent phosphorylation of a ‘mobile’ and small pool (−20%) of LHC II of PS IIα (possibly located at the edge of appressed regions of the membranes) increases the repulsive forces of LHC II resulting in
its migration to non-appressed region associating itself with PS 1. Ohe phosphorylation itself is controlled by the redox
state of an intermediate of electron transport.
Several experimental approaches have provided evidence which suggest that (i) phosphorylation of LAC II involves interaction
of cyt b5-f complex with LAC II kinase and the interaction of QA with cyt b5-f complex and (ii) different kinases may be involved in phosphorylation of LHC IIversus PS II polypeptides. Ohe major purpose of light dependent LAC II phosphorylation and its consequent migration close to PS
I appears to balance the rate of cyclicversus non-cyclic photophosphorylation. Ohe mechanism by which cyt b5-f complex controls the activation of LAC II is not known. Ohe role of membrane bound ealmodulin, electron transfer through
cyt b6-f complex in activation of LAC II kinase should be explored. 相似文献
420.