全文获取类型
收费全文 | 284篇 |
免费 | 21篇 |
出版年
2023年 | 2篇 |
2022年 | 6篇 |
2021年 | 9篇 |
2020年 | 5篇 |
2019年 | 6篇 |
2018年 | 15篇 |
2017年 | 12篇 |
2016年 | 13篇 |
2015年 | 20篇 |
2014年 | 11篇 |
2013年 | 25篇 |
2012年 | 28篇 |
2011年 | 21篇 |
2010年 | 10篇 |
2009年 | 10篇 |
2008年 | 16篇 |
2007年 | 11篇 |
2006年 | 14篇 |
2005年 | 10篇 |
2004年 | 7篇 |
2003年 | 5篇 |
2002年 | 6篇 |
2001年 | 1篇 |
2000年 | 5篇 |
1999年 | 11篇 |
1998年 | 6篇 |
1997年 | 3篇 |
1995年 | 1篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1972年 | 1篇 |
排序方式: 共有305条查询结果,搜索用时 15 毫秒
51.
Bharath Balu Chitra Chauhan Steven P Maher Douglas A Shoue Jessica C Kissinger Malcolm J Fraser John H Adams 《BMC microbiology》2009,9(1):83
Background
Much of thePlasmodium falciparumgenome encodes hypothetical proteins with limited homology to other organisms. A lack of robust tools for genetic manipulation of the parasite limits functional analysis of these hypothetical proteins and other aspects of thePlasmodiumgenome. Transposon mutagenesis has been used widely to identify gene functions in many organisms and would be extremely valuable for functional analysis of thePlasmodiumgenome. 相似文献52.
Bharath Kumar Velmurugan Lohanathan Bharathi Priya Paramasivan Poornima Li-Jen Lee Rathinasamy Baskaran 《Journal of cellular physiology》2019,234(6):8443-8454
Engineering/reprogramming differentiated adult somatic cells to gain the ability to differentiate into any type of cell lineage are called as induced pluripotent stem cells (iPSCs). Offering unlimited self-renewal and differentiation potential, these iPSC are aspired to meet the growing demands in the field of regenerative medicine, tissue engineering, disease modeling, nanotechnology, and drug discovery. Biomaterial fabrication with the rapid evolution of technology increased their versatility and utility in regenerative medicine and tissue engineering, revolutionizing the stem cell biology research with the property to guide the process of proliferation, differentiation, and morphogenesis. Combining traditional culture platforms of iPSC with biomaterials aids to overcome the limitations associated with derivation, proliferation, and maturation, thereby could improve the clinical translation of iPSC. The present review discusses in brief about the reprogramming techniques for the derivation iPSC and details on several biomaterial guided differentiation of iPSC to different cell types with specific relevance to tissue engineering/regenerative medicine. 相似文献
53.
54.
To determine whether there is a direct correlation between endurance capacity and cold tolerance, maximal oxygen consumption (VO2max), and cold-induced vasodilatation (CIVD), we measured these factors in 14 young female athletes born in Hokkaido, Japan's northernmost island. We determined the VO2max by a standard incremental test on a cycle ergometer and measured the oxygen consumption (VO2) by means of the Douglas-bag method. We determined the CIVD reaction by measuring the skin temperature of the left middle finger during immersion in cold water at 0°C for 20 min. The athletes showed significant positive correlations between VO2max, expressed as l/min, and CIVD as well as other peripheral cold tolerance indexes (resistance index against frostbite and CIVD index). The body weight VO2max (VO2max/kg body weight) failed to correlate significantly with either the CIVD or with other cold tolerance indexes. These results suggest that CIVD in females may depend on factors other than those determined in this study, in addition to the functional spread of the vascular beds in peripheral tissues, including striated muscle; it is known that the size and the vascular bed in this tissue are affected by exercise training and that this results in the elevation of VO2max and VO2max/kg body weight. 相似文献
55.
56.
Yoshiaki Maejima Koya Kushimoto Yusuke Muraguchi Kohei Fukuda Takamasa Miura Atsushi Yamazoe 《Bioscience, biotechnology, and biochemistry》2018,82(7):1260-1263
141 filterable bacteria that passed through a 0.22 μm pore size filter were isolated from Lake Sanaru in Hamamatsu, Japan. These belonged to Proteobacteria, Bacteroidetes, Firmicutes, or Actinobacteria among which the first two phyla comprised the majority of the isolates. 48 isolates (12 taxa) are candidates assignable to new bacterial species or genera of Proteobacteria or Bacteroidetes. 相似文献
57.
Kyoko Inagaki-Ohara Tetsu Sakai Goyo Koya Akira Awaya Yasunobu Yoshikai 《Microbiology and immunology》1997,41(11):883-889
We have previously reported that a nonapeptide thymic hormone, facteur thymique serique (FTS), is involved in the differentiation and activation of intestinal intraepithelial lymphocytes (i-IEL) in mice. In this study, we examined the effect of FTS treatment on enteropathy in a murine model for acute graft-vs.-host disease (GVHD) induced by injection of parental C57BL/6 splenocytes into unirradiated (C57BL/6XDBA/2) F1 hybrids. FTS treatment significantly protected mice from developing acute GVHD as assessed by mortality rate, splenomegaly and enteropathy. The infiltration of donor-derived TCRαβ i-IEL bearing CD8αβ was significantly inhibited in the small intestine of FTS-treated mice, and the frequencies of apoptosis of crypt cells in the intestinal mucosa were decreased in these mice during acute GVHD. These results suggest that FTS treatment contributes to protection against enteropathy of acute GVHD. Thus, FTS may provide a useful approach to control acute GVHD after blood transfusion or bone marrow transplantation. 相似文献
58.
McCarty L. S. Ozburn G. W. Smith A. D. Bharath A. Orr D. Dixon D. G. 《Hydrobiologia》1989,188(1):533-542
The paper examines the significance of toxicant kinetics information obtained from aquatic toxicity bioassays and bioconcentration tests. The data, bioconcentration kinetics and acute mortality versus exposure-duration information for juvenile American flagfish (Jordanella foridae) exposed to 1,4-dichlorobenzene, are interpreted in terms of a one-compartment, first-order kinetics model. The output of the model is used to formulate a testable hypothesis regarding the comparison of toxicant kinetics derived from both bioconcentration test exposures and toxicity bioassays. The model's estimates of the toxicant body burden attained at mortality are compared with theoretical and observed body burdens from literature sources. The use of a simple, deterministic residue-based, one-compartment, first-order kinetics model to evaluate existing data, as well as to formulate hypotheses to direct experimental designs, is examined. 相似文献
59.
60.
cN-II class of 5' purine nucleotidases exhibit specificity for IMP/GMP and belong to the HAD (haloacid dehalogenase) superfamily of hydrolases. The recently identified ISNI class of IMP specific 5'-nucleotidases occurring in yeast, fungi and certain Plasmodia lack sequence homology with the cN-II class of enzymes. We show from analysis of motif and fold conservation that ISN1s also belong to the HAD superfamily. This identification adds a new novel member to this superfamily. 相似文献