排序方式: 共有42条查询结果,搜索用时 15 毫秒
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Harikrishnan LS Kamau MG Wan H Inghrim JA Zimmermann K Sang X Mastalerz HA Johnson WL Zhang G Lombardo LJ Poss MA Trainor GL Tokarski JS Lorenzi MV You D Gottardis MM Baldwin KF Lippy J Nirschl DS Qiu R Miller AV Khan J Sack JS Purandare AV 《Bioorganic & medicinal chemistry letters》2011,21(5):1425-1428
SAR studies of pyrrolo[1,2-f]triazines as JAK2 inhibitors is presented. Achieving JAK2 inhibition selectively over JAK3 is discussed. 相似文献
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Shu Jiang Longmei Zhao Bhamini Purandare Basil M. Hantash 《Histochemistry and cell biology》2010,133(4):455-465
Although skin contains a number of stem cell repositories, their characterization has been hindered by a lack of specific
markers and an unclear in vivo localization. In this study, we whole mounted single human scalp hair follicles and examined
their profiles using in situ immunohistochemistry and multicolor immunofluorescence in search of markers to distinguish between
stem cells residing in the interfollicular epidermis (IFE) and bulge. Our study revealed that expression of several biomarkers
localized uniquely to the basal IFE (CD34 and CD117), bulge region (CD200), or both (CK15, CD49f, and CD29). In addition,
we found that both basal IFE and bulge stem cells did not express CD71 or CD24 suggesting their potential utility as negative
selection markers. Dermal papilla but not basal IFE or bulge stem cells expressed CD90, making it a potential positive selection
marker for dermal hair follicle stem cells. The markers tested in this study may enable pursuit of cell sorting and purification
strategies aimed at determining each stem cell population’s unique molecular signature. 相似文献
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Yarrow JF Conover CF Purandare AV Bhakta AM Zheng N Conrad B Altman MK Franz SE Wronski TJ Borst SE 《American journal of physiology. Endocrinology and metabolism》2008,295(5):E1213-E1222
High-dose testosterone enanthate (TE) may prevent hypogonadism-induced osteopenia. For this study, 3-mo-old male and female Fisher SAS rats underwent sham surgery, gonadectomy (GX), or GX plus 28 days TE administration (7.0 mg/wk). GX reduced serum sex hormones (i.e., testosterone, dihydrotestosterone, and estradiol) (P < 0.05) in both sexes and bone concentrations of testosterone (males only), and estradiol (females only). GX also elevated urine deoxypyridinoline/creatinine in both sexes and serum osteocalcin (females only), findings that are consistent with high-turnover osteopenia. GX reduced cancellous bone volume (CBV) and increased osteoid surfaces in tibia of both sexes. GX males also experienced reduced trabecular number and width and increased trabecular separation, whereas GX females experienced increased osteoblast and osteoid surfaces. Bone biomechanical characteristics remained unaffected by GX, except that femoral stiffness was reduced in females. In contrast, TE administration to GX rats elevated serum and bone androgens to supraphysiological concentrations in both sexes but altered neither serum nor bone estradiol in males. Additionally, TE did not prevent GX-induced reductions in serum or bone estradiol in females. TE also reduced markers of high-turnover osteopenia in both sexes. In males, TE prevented GX-induced changes in trabecular number and separation, CBV, and osteoid surfaces while diminishing osteoblast and osteoclast surfaces; however, these changes were not fully prevented in females. In both sexes, TE increased femoral length and femoral maximal strength to above that of Sham and GX animals while preventing the loss of femoral stiffness in females. In conclusion, TE administration appears protective of cancellous bone in male rats and augments cortical bone strength in both sexes. 相似文献
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Vaidya MM Sawant SS Borges AM Naresh NK Purandare MC Bhisey AN 《Journal of biosciences》2000,25(3):235-242
Expression of cytokeratins (CK), a subset of intermediate filament (IF) proteins in epithelia, is developmentally regulated.
CK expression may also change after malignant transformation. Our earlier studies on CK expression in human oral tumours and
pre-cancerous lesions have shown specific changes in CK expression. We analysed CK expression in human tongue and buccal mucosa
(BM) in fetuses in the embryonic age group of 16 to 27 weeks using biochemical and immunohistochemical techniques to find
out whether there is any similarity in CK expression in human oral squamous cell carcinomas (SCC) and fetal oral tissues.
CK 1, 8 and 18 were detected in a majority of samples using both techniques. Our earlier studies had shown aberrant expression
of CK 1 and 18 in many of the oral SCC and leukoplakias. Studies by immunohistochemistry showed that these different CK antigens
were expressed in different cell layers. CK 1(2) were present in the stratified epithelial layers whereas CK 8 and 18 were
restricted to glandular epithelium. Till 27 weeks of gestation, both tongue and BM expressed CK 1, 8 and 18 along with CK
6 and 16. Thus, fetal tissues showed some similarities in CK pattern with their respective SCC. 相似文献
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Vanita Noronha Kumar Prabhash Abhishek Thavamani Anuradha Chougule Nilendu Purandare Amit Joshi Rashmi Sharma Saral Desai Nirmala Jambekar Amit Dutt Rita Mulherkar 《PloS one》2013,8(4)
Screening for EGFR mutation is a key molecular test for management of lung cancer patients. Outcome of patients with mutation receiving EGFR tyrosine kinase inhibitor is known to be better across different ethnic populations. However, frequency of EGFR mutations and the clinical response in most other ethnic populations, including India, remains to be explored. We conducted a retrospective analysis of Indian lung cancer patients who were managed with oral tyrosine kinase inhibitors. Majority of the patients in the study had adenocarcinoma and were non-smokers. 39/111 patients tested positive for EGFR kinase domain mutations determined by Taqman based real time PCR. The overall response to oral TKI therapy was 30%. Patients with an activating mutation of EGFR had a response rate of 74%, while the response rate in patients with wild type EGFR was 5%, which was a statistically significant difference. Progression free survival of patients with EGFR mutations was 10 months compared to 2 months for EGFR mutation negative patients. Overall survival was 19 months for EGFR mutation patients and 13 months for mutation negative patients. This study emphasizes EGFR mutation as an important predictive marker for response to oral tyrosine kinase inhibitors in the Indian population. 相似文献
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