全文获取类型
收费全文 | 443篇 |
免费 | 30篇 |
出版年
2023年 | 7篇 |
2022年 | 10篇 |
2021年 | 20篇 |
2020年 | 8篇 |
2019年 | 15篇 |
2018年 | 18篇 |
2017年 | 27篇 |
2016年 | 26篇 |
2015年 | 25篇 |
2014年 | 32篇 |
2013年 | 30篇 |
2012年 | 34篇 |
2011年 | 42篇 |
2010年 | 16篇 |
2009年 | 17篇 |
2008年 | 19篇 |
2007年 | 23篇 |
2006年 | 18篇 |
2005年 | 8篇 |
2004年 | 14篇 |
2003年 | 9篇 |
2002年 | 4篇 |
2001年 | 8篇 |
2000年 | 2篇 |
1999年 | 1篇 |
1998年 | 2篇 |
1997年 | 3篇 |
1996年 | 2篇 |
1995年 | 3篇 |
1994年 | 3篇 |
1993年 | 4篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 3篇 |
1979年 | 2篇 |
1978年 | 1篇 |
1976年 | 2篇 |
1973年 | 1篇 |
1968年 | 1篇 |
1965年 | 1篇 |
排序方式: 共有473条查询结果,搜索用时 15 毫秒
51.
Species-specific codon context rules unveil non-neutrality effects of synonymous mutations 总被引:1,自引:0,他引:1
Moura GR Pinheiro M Freitas A Oliveira JL Frommlet JC Carreto L Soares AR Bezerra AR Santos MA 《PloS one》2011,6(10):e26817
BackgroundCodon pair usage (codon context) is a species specific gene primary structure feature whose evolutionary and functional roles are poorly understood. The data available show that codon-context has direct impact on both translation accuracy and efficiency, but one does not yet understand how it affects these two translation variables or whether context biases shape gene evolution.ConclusionsSince in vivo studies provide evidence for a role of codon context on decoding fidelity in E. coli and for decoding efficiency in mammalian cells, our data support the hypothesis that, like codon usage, codon context modulates the evolution of gene primary structure and fine tunes the structure of open reading frames for high genome translational fidelity and efficiency in the 3 domains of life. 相似文献
52.
Bezerra GB Cançado GM Menossi M Castro LN Von Zuben FJ 《Genetics and molecular research : GMR》2005,4(3):514-524
Several advanced techniques have been proposed for data clustering and many of them have been applied to gene expression data, with partial success. The high dimensionality and the multitude of admissible perspectives for data analysis of gene expression require additional computational resources, such as hierarchical structures and dynamic allocation of resources. We present an immune-inspired hierarchical clustering device, called hierarchical artificial immune network (HaiNet), especially devoted to the analysis of gene expression data. This technique was applied to a newly generated data set, involving maize plants exposed to different aluminum concentrations. The performance of the algorithm was compared with that of a self-organizing map, which is commonly adopted to deal with gene expression data sets. More consistent and informative results were obtained with HaiNet. 相似文献
53.
Background
The tear film is a thin layer of fluid that covers the ocular surface and is involved in lubrication and protection of the eye. Little is known about the protein composition of tear fluid but its deregulation is associated with disease states, such as diabetic dry eyes. This makes this body fluid an interesting candidate for in-depth proteomic analysis. 相似文献54.
55.
de Moura Júnior NB das-Neves-Pereira JC de Campos JR de Oliveira FR Wolosker N Parra ER Capelozzi VL Jatene FB 《Molecular neurobiology》2012,45(2):362-365
The goal of this study was to evaluate if the immunohistochemical expression of alpha-3 neuronal nicotinic acetylcholine receptor
subunit in sympathetic ganglia remains stable after brain death, determining the possible use of sympathetic thoracic ganglia
from subjects after brain death as study group. The third left sympathetic ganglion was resected from patients divided in
two groups: BD—organ donors after brain death and CON—patients submitted to sympathectomy for hyperhidrosis (control group).
Immunohistochemical staining for alpha-3 neuronal nicotinic acetylcholine receptor subunit was performed; strong and weak
expression areas were quantified in both groups. The BD group showed strong alpha-3 neuronal nicotinic acetylcholine receptor
expression in 6.55% of the total area, whereas the CON group showed strong expression in 5.91% (p = 0.78). Weak expression was found in 6.47% of brain-dead subjects and in 7.23% of control subjects (p = 0.31). Brain death did not affect the results of the immunohistochemical analysis of sympathetic ganglia, and its use as
study group is feasible. 相似文献
56.
57.
GA Bezerra E Dobrovetsky A Dong A Seitova L Crombett LM Shewchuk AM Hassell SM Sweitzer TD Sweitzer PJ McDevitt KO Johanson KM Kennedy-Wilson D Cossar A Bochkarev K Gruber S Dhe-Paganon 《PloS one》2012,7(8):e43019
Proline-specific dipeptidyl peptidases (DPPs) are emerging targets for drug development. DPP4 inhibitors are approved in many countries, and other dipeptidyl peptidases are often referred to as DPP4 activity- and/or structure-homologues (DASH). Members of the DASH family have overlapping substrate specificities, and, even though they share low sequence identity, therapeutic or clinical cross-reactivity is a concern. Here, we report the structure of human DPP7 and its complex with a selective inhibitor Dab-Pip (L-2,4-diaminobutyryl-piperidinamide) and compare it with that of DPP4. Both enzymes share a common catalytic domain (α/β-hydrolase). The catalytic pocket is located in the interior of DPP7, deep inside the cleft between the two domains. Substrates might access the active site via a narrow tunnel. The DPP7 catalytic triad is completely conserved and comprises Ser162, Asp418 and His443 (corresponding to Ser630, Asp708 and His740 in DPP4), while other residues lining the catalytic pockets differ considerably. The "specificity domains" are structurally also completely different exhibiting a β-propeller fold in DPP4 compared to a rare, completely helical fold in DPP7. Comparing the structures of DPP7 and DPP4 allows the design of specific inhibitors and thus the development of less cross-reactive drugs. Furthermore, the reported DPP7 structures shed some light onto the evolutionary relationship of prolyl-specific peptidases through the analysis of the architectural organization of their domains. 相似文献
58.
Bruna Martins Bezerra Antonio da Silva Souto Lewis George Halsey Nicola Schiel 《Journal of Ethology》2008,26(1):175-178
Bradypus variegatus is a member of the Order Pilosa, Family Bradypodidae, and is distributed in many subtropical and tropical countries in South
and Central America. However, studies on this species in the wild are relatively limited and many aspects of its reproductive
behaviour are unknown or unclear. The current report presents new observations of the reproductive behaviour of B. variegatus in its natural environment. These include details of both a male–female copulation and the simultaneous nurturing of two young
sloths. 相似文献
59.
Mitracarpus longicalyx is here described and illustrated from sandy areas of the caatingas of northeastern Brazil. The new species is similar toM. hirtus andM. megapotamicus. 相似文献
60.