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Robert Lehmann Damien J. Lightfoot Celia Schunter Craig T. Michell Hajime Ohyanagi Katsuhiko Mineta Sylvain Foret Michael L. Berumen David J. Miller Manuel Aranda Takashi Gojobori Philip L. Munday Timothy Ravasi 《Molecular ecology resources》2019,19(3):570-585
The iconic orange clownfish, Amphiprion percula, is a model organism for studying the ecology and evolution of reef fishes, including patterns of population connectivity, sex change, social organization, habitat selection and adaptation to climate change. Notably, the orange clownfish is the only reef fish for which a complete larval dispersal kernel has been established and was the first fish species for which it was demonstrated that antipredator responses of reef fishes could be impaired by ocean acidification. Despite its importance, molecular resources for this species remain scarce and until now it lacked a reference genome assembly. Here, we present a de novo chromosome‐scale assembly of the genome of the orange clownfish Amphiprion percula. We utilized single‐molecule real‐time sequencing technology from Pacific Biosciences to produce an initial polished assembly comprised of 1,414 contigs, with a contig N50 length of 1.86 Mb. Using Hi‐C‐based chromatin contact maps, 98% of the genome assembly were placed into 24 chromosomes, resulting in a final assembly of 908.8 Mb in length with contig and scaffold N50s of 3.12 and 38.4 Mb, respectively. This makes it one of the most contiguous and complete fish genome assemblies currently available. The genome was annotated with 26,597 protein‐coding genes and contains 96% of the core set of conserved actinopterygian orthologs. The availability of this reference genome assembly as a community resource will further strengthen the role of the orange clownfish as a model species for research on the ecology and evolution of reef fishes. 相似文献
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Global species delimitation and phylogeography of the circumtropical ‘sexy shrimp’ Thor amboinensis reveals a cryptic species complex and secondary contact in the Indo‐West Pacific 下载免费PDF全文
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When biogeographical provinces collide: hybridization of reef fishes at the crossroads of marine biogeographical provinces in the Arabian Sea 下载免费PDF全文
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Large‐scale,multidirectional larval connectivity among coral reef fish populations in the Great Barrier Reef Marine Park 下载免费PDF全文
David H. Williamson Hugo B. Harrison Glenn R. Almany Michael L. Berumen Michael Bode Mary C. Bonin Severine Choukroun Peter J. Doherty Ashley J. Frisch Pablo Saenz‐Agudelo Geoffrey P. Jones 《Molecular ecology》2016,25(24):6039-6054
Larval dispersal is the key process by which populations of most marine fishes and invertebrates are connected and replenished. Advances in larval tagging and genetics have enhanced our capacity to track larval dispersal, assess scales of population connectivity, and quantify larval exchange among no‐take marine reserves and fished areas. Recent studies have found that reserves can be a significant source of recruits for populations up to 40 km away, but the scale and direction of larval connectivity across larger seascapes remain unknown. Here, we apply genetic parentage analysis to investigate larval dispersal patterns for two exploited coral reef groupers (Plectropomus maculatus and Plectropomus leopardus) within and among three clusters of reefs separated by 60–220 km within the Great Barrier Reef Marine Park, Australia. A total of 69 juvenile P. maculatus and 17 juvenile P. leopardus (representing 6% and 9% of the total juveniles sampled, respectively) were genetically assigned to parent individuals on reefs within the study area. We identified both short‐distance larval dispersal within regions (200 m to 50 km) and long‐distance, multidirectional dispersal of up to ~250 km among regions. Dispersal strength declined significantly with distance, with best‐fit dispersal kernels estimating median dispersal distances of ~110 km for P. maculatus and ~190 km for P. leopardus. Larval exchange among reefs demonstrates that established reserves form a highly connected network and contribute larvae for the replenishment of fished reefs at multiple spatial scales. Our findings highlight the potential for long‐distance dispersal in an important group of reef fishes, and provide further evidence that effectively protected reserves can yield recruitment and sustainability benefits for exploited fish populations. 相似文献
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Using a pair of congenic strains of mice differing only at the Mls haplotype (Mls locus and closely linked genes), BALB/c (Mls
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) and BALB.D2-Mls
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, we have compared the in vitro proliferative responses of M1sb lymphocytes to M1sa antigens presented on either lymph node cells (LNC) or peritoneal adherent cells (PAC). Results showed that M1sa-PAC are stronger stimulators than M1sa-LNC, and furthermore, that the supernatant from M1sa-PAC may be effective in eliciting a lymphocyte proliferative response. The proliferation in response to PAC supernatant is partially due to activation by nonspecific factor(s); however, the response in the presence of M1sa incompatible PAC supernatant is about three times greater than the response obtained in the presence of syngeneic M1sb-PAC supernatant, suggesting an additional stimulation by soluble M1sa antigens. Contrasting with the ability of PAC-supernatant to stimulate a primary proliferative response in vitro, the in vivo immunization of Mlsb mice with M1sa-PAC supernatant abrogates the specific proliferative response in subsequent one-way mixed lymphocyte cultures. This abrogation of the specific response is comparable to that observed after immunization with intact M1sa peritoneal or spleen cells, although in the latter case the anti-H-2 proliferative response is also decreased, regardless of whether the H-2 incompatible stimulating cells express an additional incompatibility for M1sa. The proliferation of untreated, but not of M1sa-immunized BALB/c LNC, is stronger in cultures with DBA/2 stimulating cells (incompatible for M1sa and other non-H-2 antigens) than in cultures with BALBM-Mls
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cells (incompatible for M1sa alone), and is comparable in intensity to that activated by H-2 incompatibility. We conclude that M1sa antigens are more efficiently recognized by unprimed helper T cells when presented on PAC than when presented on LNC. In the primary proliferative response, the effects of M1sa and other non-H-2 antigens may be cumulative. In vivo immunization against M1sa antigens results in suppression of the specific proliferative response and, to a certain extent, of the nonspecific proliferative response (directed against both H-2 and other non-H-2 antigens). Since M1sa antigens are obtainable in soluble form, their physicochemical purification can now be envisaged. 相似文献
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