全文获取类型
收费全文 | 2707篇 |
免费 | 237篇 |
国内免费 | 1篇 |
出版年
2023年 | 9篇 |
2022年 | 23篇 |
2021年 | 39篇 |
2020年 | 22篇 |
2019年 | 31篇 |
2018年 | 43篇 |
2017年 | 35篇 |
2016年 | 66篇 |
2015年 | 98篇 |
2014年 | 143篇 |
2013年 | 131篇 |
2012年 | 161篇 |
2011年 | 193篇 |
2010年 | 137篇 |
2009年 | 113篇 |
2008年 | 138篇 |
2007年 | 143篇 |
2006年 | 149篇 |
2005年 | 104篇 |
2004年 | 143篇 |
2003年 | 127篇 |
2002年 | 126篇 |
2001年 | 56篇 |
2000年 | 56篇 |
1999年 | 50篇 |
1998年 | 40篇 |
1997年 | 32篇 |
1996年 | 34篇 |
1995年 | 15篇 |
1994年 | 26篇 |
1993年 | 26篇 |
1992年 | 37篇 |
1991年 | 25篇 |
1990年 | 31篇 |
1989年 | 30篇 |
1988年 | 25篇 |
1987年 | 18篇 |
1986年 | 26篇 |
1985年 | 28篇 |
1984年 | 21篇 |
1983年 | 19篇 |
1982年 | 17篇 |
1981年 | 11篇 |
1980年 | 8篇 |
1979年 | 24篇 |
1978年 | 20篇 |
1977年 | 10篇 |
1976年 | 11篇 |
1974年 | 6篇 |
1972年 | 11篇 |
排序方式: 共有2945条查询结果,搜索用时 15 毫秒
991.
992.
Rouyer Tristan Bernard Serge Kerzerho Vincent Giordano Nicolas Giordano François Ellul Salvu Ellul Giovanni Derridj Olivier Canet Rémy Deguara Simeon Wendling Bertrand Bonhommeau Sylvain 《Environmental Biology of Fishes》2022,105(5):635-644
Environmental Biology of Fishes - The purse seine fishery in the Mediterranean represents about 60% of the international catch for Atlantic Bluefin Tuna (Thunnus thynnus). Yet, tagging operations... 相似文献
993.
994.
Cécile Boland Sophie Bertrand Wesley Mattheus Katelijne Dierick Vicky Jasson Toon Rosseel Steven Van Borm Jacques Mahillon Pierre Wattiau 《Applied and environmental microbiology》2015,81(9):3169-3175
Fifty-nine monophasic Salmonella enterica serovar Typhimurium isolates, collected in Belgium during the period from 2008 to 2011, have been serotyped as 4,[5]:i:− and shown to harbor an fljB coding sequence. The genetic differences between these strains and phenotypically biphasic Salmonella Typhimurium were analyzed through PCR and DNA sequencing. Genetic alterations in the fljB promoter region affecting expression of the phase 2 flagellin were observed in 53 isolates. Other genetic events in the invertible region carrying the fljB promoter were observed in 2 isolates. For the remaining 4 isolates, no molecular differences with a reference biphasic Salmonella Typhimurium strain could be observed. Next-generation sequencing of one representative isolate affected in the fljB promoter region revealed a 26-kb IS26 composite transposon insertion along with a local genomic rearrangement. Several other IS26 element-mediated alterations of this genomic region were observed. This group of monophasic Salmonella Typhimurium isolates was genetically heterogeneous, as revealed by multilocus variable-number tandem-repeat analysis (MLVA), PCR, and sequencing. Pigs and pork represented a major source of such monophasic isolates in Belgium, as reported in other countries. Three out of 5 isolates of human origin presented genetic profiles identical to those of food isolates, demonstrating the pathogenic potential of the newly characterized variants and potential dissemination along the food chain. This study highlighted the key role played by IS26 insertions in the loss of phase 2 flagellin expression and the subsequent generation of multiple monophasic variant lineages from biphasic Salmonella Typhimurium ancestors. 相似文献
995.
Morphological and plumage colour variation in the Réunion grey white‐eye (Aves: Zosterops borbonicus): assessing the role of selection
下载免费PDF全文
![点击此处可从《Biological journal of the Linnean Society. Linnean Society of London》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Joris A. M. Bertrand Yann X. C. Bourgeois Borja Milá Philipp Heeb Christophe Thébaud 《Biological journal of the Linnean Society. Linnean Society of London》2015,114(2):459-473
The Réunion grey white‐eye (Zosterops borbonicus), a small passerine endemic to the island of Réunion (Mascarene archipelago), constitutes an extraordinary case of phenotypic variation within a bird species, with conspicuous plumage colour differentiation at a microgeographical scale. To understand whether natural selection could explain such variability, we compared patterns of variation in morphological and plumage colour traits within and among populations. To quantify morphological variation, we used measurements obtained by Frank Gill in the 1960s from 239 individuals collected in 60 localities distributed over the entire island of Réunion. To quantify colour variation, we measured the reflectance spectra of plumage patches of 50 males from a subset of Gill's specimens belonging to the five recognized plumage colour variants and used a visual model to project these colours in an avian‐appropriate, tetrachromatic, colour space. We found that variants occupy different regions of the avian colour space and that between‐variant differences for most plumage patches could be discriminated by the birds. Differences in morphology were also detected, but these were, in general, smaller than colour differences. Overall, we found that variation in both plumage colour and morphology among variants is greater than would be expected if genetic drift alone was responsible for phenotypic divergence. As the plumage colour variants correspond to four geographical forms, our results suggest that phenotypic evolution in the Réunion grey white‐eye is at least partly explained by divergent selection in different habitats or regions. © 2014 The Linnean Society of London, Biological Journal of the Linnean Society, 2015, 114 , 459–473. 相似文献
996.
J A M Bertrand Y X C Bourgeois B Delahaie T Duval R García-Jiménez J Cornuault P Heeb B Milá B Pujol C Thébaud 《Heredity》2014,112(2):190-196
The Réunion grey white-eye, Zosterops borbonicus, a passerine bird
endemic to Réunion Island in the Mascarene archipelago, represents an extreme case
of microgeographical plumage colour variation in birds, with four distinct colour forms
occupying different parts of this small island (2512 km2). To understand
whether such population differentiation may reflect low levels of dispersal and gene flow
at a very small spatial scale, we examined population structure and gene flow by analysing
variation at 11 microsatellite loci among four geographically close localities
(<26 km apart) sampled within the distribution range of one of the colour forms,
the brown-headed brown form. Our results revealed levels of genetic differentiation that
are exceptionally high for birds at such a small spatial scale. This strong population
structure appears to reflect low levels of historical and contemporary gene flow among
populations, unless very close geographically (<10 km). Thus, we suggest that
the Réunion grey white-eye shows an extremely reduced propensity to disperse, which
is likely to be related to behavioural processes. 相似文献
997.
998.
Yvan Martineau Xiaoshan Wang Tommy Alain Emmanuel Petroulakis David Shahbazian Bertrand Fabre Marie-Pierre Bousquet-Dubouch Bernard Monsarrat Stéphane Pyronnet Nahum Sonenberg 《Molecular and cellular biology》2014,34(6):1046-1053
The simultaneous interaction of poly(A)-binding protein (PABP) with eukaryotic translation initiation factor 4G (eIF4G) and the mRNA 3′ poly(A) tail promotes translation initiation. We previously showed that the interaction of PABP-interacting protein 1 (Paip1) with PABP and eukaryotic translation initiation factor 3 (eIF3; via the eIF3g subunit) further stimulates translation. Here, we demonstrate that the interaction of eIF3 with Paip1 is regulated by amino acids through the mTORC1 signaling pathway. The Paip1-eIF3 interaction is impaired by the mTORC1 inhibitors, rapamycin and PP242. We show that ribosomal protein S6 kinases 1 and 2 (S6K1/2) promote the interaction of eIF3 with Paip1. The enhancement of Paip1-eIF3 interaction by amino acids is abrogated by an S6K inhibitor or shRNA against S6K1/2. S6K1 interacts with eIF3f and, in vitro, phosphorylates eIF3. Finally, we show that S6K inhibition leads to a reduction in translation by Paip1. We propose that S6K1/2 phosphorylate eIF3 to stimulate Paip1-eIF3 interaction and consequent translation initiation. Taken together, these data demonstrate that eIF3 is a new translation target of the mTOR/S6K pathway. 相似文献
999.
Laurence Wurth Anne-Sophie Gribling-Burrer Céline Verheggen Michael Leichter Akiko Takeuchi Stéphanie Baudrey Franck Martin Alain Krol Edouard Bertrand Christine Allmang 《Nucleic acids research》2014,42(13):8663-8677
Mammalian mRNAs are generated by complex and coordinated biogenesis pathways and acquire 5′-end m7G caps that play fundamental roles in processing and translation. Here we show that several selenoprotein mRNAs are not recognized efficiently by translation initiation factor eIF4E because they bear a hypermethylated cap. This cap modification is acquired via a 5′-end maturation pathway similar to that of the small nucle(ol)ar RNAs (sn- and snoRNAs). Our findings also establish that the trimethylguanosine synthase 1 (Tgs1) interacts with selenoprotein mRNAs for cap hypermethylation and that assembly chaperones and core proteins devoted to sn- and snoRNP maturation contribute to recruiting Tgs1 to selenoprotein mRNPs. We further demonstrate that the hypermethylated-capped selenoprotein mRNAs localize to the cytoplasm, are associated with polysomes and thus translated. Moreover, we found that the activity of Tgs1, but not of eIF4E, is required for the synthesis of the GPx1 selenoprotein in vivo. 相似文献
1000.