H NOE studies of oxidized high potential iron sulfur protein II from Ectothiorhodospira halophila

The ¹H NMR spectra of oxidized HiPIP II from Ectothiorhodospira halophila have been recorded at 600 MHz. Nuclear Overhauser effect measurements have allowed the assignment of the cysteine β-CH₂ resonances. Four β-CH₂ signals are downfield shifted and four upfield shifted. Through a theoretical model and on the ground of Mössbauer data on analogous systems we propose that the upfield signals are those of the cysteines bound to the iron(III) ions and those downfield of the cysteines bound to the mixed valence pair Fe(III)-Fe(II).     

Induction of indoleamine dioxygenase by interferon in mice: a study with different recombinant interferons and various cytokines

Since it is important the availability of a specific marker for interferon induction in vivo, we investigated the effect of different recombinant interferons and various cytokines on indoleamine 2,3-dioxygenase activity. Although with different magnitude, recombinant interferon-alpha A/D (Bgl II) hybrid, interferon-gamma and tumor necrosis factor, all increase the activity of this enzyme, whereas interleukin-1, recombinant interferon-alpha A and interferon-alpha D do not induce this activity in mice lung tissue. Dexamethasone is able to inhibit indoleamine 2,3-dioxygenase induction by lipopolysaccharide or by interferon-alpha A/D but it fails to prevent the induction by interferon-gamma.