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11.
In this study we compared the properties of cytochrome-c oxidase (COX) in cultured fibroblasts from two patients with Leigh Syndrome with COX from control fibroblasts. The fibroblasts from patients showed decreased growth reates and elevated lactate production. COX activity of patients fibroblasts was about 25% of control. Kinetic studies with isolated mitochondria showed a higher Km for cytochrome c and a markedly reduced molecular turnover of COX from patients, indicating a different structure of the enzyme. A biphasic change of COX activity was obtained by titration of dodecylmaltoside solubilized mitochondria from control fibroblasts with increasing concentrations of anions. With patient mitochondria we found only the inhibiting phase of COX activity and, in contrast to control mitochondria, irreversible inhibition of COX activity by guanidinium chloride. ELISA titrations with monoclonal antibodies to subunit II, IV, Vab, VIac and VIIab indicated a normal amount of mitochondrial coded subunit II, but a reduced amound of nuclear coded subunits. The data indicate incompletely assembled nuclear coded subunits of COX from patient fibroblasts. 相似文献
12.
Chromophoric [4-(dimethylamino)cinnamoyl]imidazole reacts with the serine protease alpha-chymotrypsin to form an acyl enzyme. At pHs below 4.0, the acyl enzyme turns over very slowly to yield the free acid. During this slow deacylation it is possible to obtain a very good resonance Raman spectrum of the acyl intermediate by using the 350.7-nm line of the krypton laser. The resonance Raman carbonyl frequency of the covalently bonded substrate and its wavelength at maximum intensity in the absorption spectrum of the acyl enzyme have been taken and used to monitor the active site environment. A comparison has been made of the absorption and Raman spectra of the acyl enzyme and those of the corresponding chromophoric methyl ester, aldehyde, and imidazole model compounds. A linear correlation is found between the wavelength of maximum absorption and the Raman frequency of the carbonyl group over a wide range of solvent conditions for each of the model compounds. By combining the Raman carbonyl frequency with the absorption maximum, we can determine that the bond order changes in the carbonyl bond of the bound substrate are not due to changes in the solvent, since the carbonyl frequency and the absorption maximum of the acyl enzyme do not fall on any of the linear correlations for the model compounds. The unusual spectroscopic properties of the bound substrate appear to be due to some specific enzyme-induced change in the substrate when it is bound at the active site. Thermal unfolding of the acyl enzymes changes both the carbonyl frequency of the acyl enzyme and its absorption maximum to completely different values.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
13.
Bernhard Knig Patricia A. DiNitto Peter M. Blumberg 《Journal of cellular biochemistry》1985,29(1):37-44
The major phorbol ester receptor is the Ca++-activated, phospholipid-dependent protein kinase C. Diacylglycerol stimulates protein kinase C in a fashion similar to the phorbol esters. Likewise, it inhibits phorbol ester binding competitively. Both results suggest that diacylglycerol is the/an endogenous phorbol ester analogue. Alternatively, the diacylglycerol might simply be acting to modify the phospholipid environment of the protein. If diacylglycerol were indeed functioning as an analogue, it should interact with the receptor stoichiometrically. This interaction can be quantitated by measuring the perturbation in apparent diacylglycerol binding affinity as a function of the ratio of diacylglycerol to receptor. We report here that 1,2-dioleoylglycerol interacts with the receptor with the predicted stoichiometry. 相似文献
14.
Bernhard Reinicke Peter Blümel Harald Labischinski Peter Giesbrecht 《Archives of microbiology》1985,141(4):309-314
In contrast to what has been postulated, penicillin G at its optimal lytic concentration of 0.1 g per ml did not lead to a detectable activation of autolytic wall processes in staphylococci in terms of the release of uniformly labelled wall fragments from cells pretreated with the drug for 1 h. Rather a considerable inhibition of this release was observed. A similarly profound inhibition of the release of peptidoglycan fragments occurred when staphylococci pretreated for 1 h with 0.1 g penicillin per ml acted as a source of crude autolysins on peptidoglycan isolated from labelled normal cells of the same strain. This clearly demonstrated that the overall inhibition of autolytic wall processes caused by penicillin was mainly due to a decreased total autolysin action rather than to an altered wall structure. Furthermore, no substantial penicillin-induced inhibition of the incorporation of 14C-N-acetylglucosamine into the staphylococcal wall could be observed before bacteriolysis started, i. e., approximately during the first 80 min of penicillin action. These results are not consistent with any of the models hitherto proposed for the action of penicillin.Dedicated to Prof. Dr. Gerhart Drews on the occasion of his 60th birthday 相似文献
15.
W Bernhard 《Anthropologischer Anzeiger; Bericht über die biologisch-anthropologische Literatur》1983,41(2):93-110
An attempt has been made to illustrate the quite complicated process of ethnogenesis in South Asia from the viewpoint of physical anthropology. The numerous invading waves which reached the Indian subcontinent from the northwest played an important role in this process. Most important for the ethnogenesis of South Asia was the invasion of Indo-Aryan groups in the middle of the 2nd millenium B.C. known from historical sources. In large parts of the Indo-Pakistan region they assimilated the aboriginal population in ethnic, cultural and linguistic respects in the course of time. Furthermore, the ethnogenesis of the Indian region is determined by the caste system of Hinduism which, however, is not as rigid as generally assumed. There are numerous evidences that since more than 2000 years a slow but steady process of assimilation and integration of tribal groups, living in the forest areas of Central India, into the Hindu caste system took place, a process which is still going on. It is intended to demonstrate to what degree the ethnogenetic processes in South Asia, known from prehistoric and historical sources, can be traced in human skeletal findings of different time periods as well as in the anthropological structure of the living population. Finally, hypotheses and theories, especially those of Risley and von Eickstedt are discussed, who attempted to interpret the great variability of anthropological and morphological traits in the Indian subcontinent by taking into consideration the existence of different old population substrata and their mixing and assimilation. 相似文献
16.
17.
Alterations in lymphocyte homing patterns within mice exposed to ultraviolet radiation 总被引:2,自引:0,他引:2
G J Spangrude E J Bernhard R S Ajioka R A Daynes 《Journal of immunology (Baltimore, Md. : 1950)》1983,130(6):2974-2981
This report presents the results of an investigation designed to establish whether exposure of mice to ultraviolet radiation (UVR) is capable of influencing the factors that control the distribution of lymphoid cells in vivo. We found that such exposure resulted in a dramatic and long-lasting increase in the tropism of peripheral lymph nodes for circulating lymphoid cells. Termination of UVR exposure did not result in a reversal of this phenomenon. Since an increase in lymphocyte migration into the lymph nodes of UVR-exposed mice was apparent within 2 hr of infusion of the radiolabeled cells, we conclude that the homing assay data reflect a relatively increased binding of circulating lymphocytes to high endothelial venules (HEV) within the lymph nodes of irradiated animals. A histologic analysis of skin from UVR-exposed mice established that the dermal microvasculature had expanded in terms of size and number of vessels, a condition that also does not completely reverse after the termination of treatments. In spite of the increase in dermal microvasculature, very few inflammatory cells were detected in the irradiated skin site. These observations support our conclusion that the enhanced traffic of lymphocytes into peripheral lymph nodes of UVR-exposed mice occurs primarily via lymphocyte-HEV interactions rather than afferent drainage of the irradiated skin. 相似文献
18.
A new staining procedure for electron microscopical cytology 总被引:47,自引:0,他引:47
W Bernhard 《Journal of ultrastructure research》1969,27(3):250-265
19.
Bernhard Werner 《Pal?ontologische Zeitschrift》1967,41(3-4):137-153
The scyphopolypStephanoscyphus Allman 1874 represents the polyp generation of the scyphomedusan order Coronatae. The benthonic polyp is known to occur with several species on the continental shelves and in greater depths of the oceans. Participating in the International Indian Ocean Expedition 1964 to 1965 on board of the German research vessel «Meteor», the author was able to collect living specimens. For the first time, it was possible to rear these polyps in laboratory cultures.Stephanoscyphus differs from all other living scyphopolyps by the possession of a firm periderm tube enclosing completely the soft body. It is this primitive feature whichStephanoscyphus has in common with the Conulata being the ancestors of the recent Scyphozoa. The characteristics found in a detailed investigation of the periderm tube ofStephanoscyphus conform well with those found in the tubes of the Conulata except for the closure of the aperture by triangular flaps which are absent inStephanoscyphus. The soft body contains primitive features as well. Hence from the existence, morphology and life history it must be concluded that the type of organization which the Conulata exhibited has survived inStephanoscyphus. 相似文献
20.
Bernhard Werner 《Helgoland Marine Research》1966,13(4):317-347
The scyphopolypStephanoscyphus Allman 1874 represents the polyp generation of the scyphomedusan order Coronatae. Though this polyp has been known for more than a hundred years its general morphology, systematics, and evolution have been inadequately described. Participating in the International Indian Ocean Expedition, 1964 to 1965, on board of the German research vessel “Meteor”, the author was able to collect a sufficient supply of livingStephanoscyphus off the coasts of South Arabia and East Africa. For the first time, it was possible to rear these polyps in laboratory cultures. A thorough investigation of morphology, developmental history and behaviour based on longterm observations of the living polyps gave clear indications thatStephanoscyphus directly descended from the fossil group of Conulata, the scyphozoan nature of which has been affirmed byKiderlen (1937) andKnight (1937). The main feature whichStephanoscyphus has in common with the Conulata is the possession of a periderm tube. The characteristics found in a detailed investigation of the periderm tube conform well with those found in the periderm of the Conulata except for the closure of the aperture by triangular flaps which are absent inStephanoscyphus. The soft body contains primitive features as well. Hence it must be concluded finally that the type of organization which the fossil ancestors exhibited has survived inStephanoscyphus and that the Coronatae represent the most basic group of all living Scyphozoa. On the other hand, the results give strong support for the scyphozoan nature of the Conulata, the organization and life history of which have been elucidated by the observations of the living representatives ofStephanoscyphus. 相似文献