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981.
Bernhard Henrich Ulrich Schroeder Rainer W. Frank Roland Plapp 《Molecular & general genetics : MGG》1989,215(3):369-373
Summary A cloned DNA fragment, carrying the gene for peptidase D (pepD) of Escherichia coli, was partially sequenced. By purification of peptidase D and sequence determination of an amino-terminal oligopeptide the reading frame of the pepD gene, starting with a GTG initiator codon, was unambiguously identified.An overlap of the established nucleotide sequence with the previously sequenced 5 flanking region of the gpt gene allowed the exact distance between pepD and gpt to be calculated. The two genes are pointing towards each other and are separated by 260 bp. A search for open reading frames (ORFs) and the analysis of possible codon usage in the intercistronic region indicate the absence of an additional gene (lpcA) between pepD and gpt. 相似文献
982.
Microdissection of banded human chromosomes 总被引:9,自引:3,他引:6
Gabriele Senger Hermann-Josef Lüdecke Bernhard Horsthemke Uwe Claussen 《Human genetics》1990,84(6):507-511
Summary Physical dissection of metaphase chromosomes is the most straightforward approach for the isolation of DNA sequences from specific chromosome regions. However, conventional microdissection techniques are too crude and inefficient for analysis of the human genome. Here we describe a technique for the precise dissection of single bands from GTG-banded chromosomes. Cells from normal amniotic fluid cell cultures are harvested by the pipette method. Microdissection is performed on an inverted microscope (magnification 1250 x) with the help of extended siliconized glass needles and an electronically controlled micromanipulator. Enzymatic amplification of the dissected DNA allows the construction of band-specific DNA libraries from as few as 20 dissected chromosome fragments. 相似文献
983.
Bernhard Aicher Jürgen Tautz 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1990,166(3):345-353
1. | During courtship behavior, males of the fiddler crab, Uca pugilator, drum on the ground with their large chela. The types of waves this produces and some of their properties were investigated using a laser Doppler vibrometer and accelerometers under field and laboratory conditions. |
2. | Rhythmical impact onto the substratum by Uca produces 3 types of surface waves: Rayleigh waves and Love waves which contain most of the energy, and the weaker surface P-waves. |
3. | The group velocity of Love-waves is 50–60 m/s in wet sand. Rayleigh waves travel at 70–80 m/s in wet sand and obout 40 m/s in dry sand. The propagation velocity of surface P-waves is 150–160 m/s in compact wet sand and about 140 m/s in wet sand perforated by crab burrows. The group velocity of Rayleigh and Love waves is not influenced by the presence of crab burrows. |
4. | Fast Fourier transform (FFT) spectra of single beats reveal that the energy maxima of Rayleigh and Love waves lie in the frequency range of 340–370 Hz, i.e., at much higher frequencies than the beat rate of the fiddler crabs, which is usually below 40/s. The optimal frequency is independent of the distance from the signalling male. |
5. | In the optimal frequency range, the specific damping coefficient 10 for Rayleigh waves is very low and amounts to 0.13–0.16 dB/cm in wet sand and 0.23–0.49 dB/cm in dry sand. Substrate vibrations of higher frequencies are more strongly damped. |
6. | Considering the size of a fiddler crab, the physical properties of the Rayleigh and Love waves in the optimal frequency range provide a suitable signal for localizing mechanisms which rely on time or phase differences but not on intensity or spectral differences of propagating substrate vibrations. |
984.
Half-site reactivity 总被引:3,自引:0,他引:3
985.
986.
987.
1. Using 6 phytoplankton species and/or the copepodEuterpina acutifrons or larvae of the sea urchinArbacia lixula the potential inhibitory effects of chemicals released from some 70 different materials (mainly plastics) have been tested. In addition, the effects of 6 detergents have been examined. 2. Several materials, such as natural rubbers and polyvinyl chlorides, are highly toxic and should never be used when experimenting with living marine organisms. 3. Teflon (Algoflon), Perspex, Polyethylene, Tygon, Polypropylene, Polycarbonates (Makrolon) and Polyester (Gabraster) have been shown to be non-toxic and are, therefore, suitable for use in cultivation of marine organisms. Some materials had slightly negative effects on the organisms tested and should, therefore, be used only if no alternatives are available. 4. Some suggestions are advanced on how to construct non-toxic samplers and laboratory equipment used for experiments with marine organisms. 相似文献
988.
A novel approach to determine the tetracycline susceptibility of Chlamydia trachomatis directly from specimens without cell culture propagation and adaptation has been explored. Out of a total of 1290 genital specimens from a sexually transmitted disease clinic, 211 (16.4%) were positive for C. trachomatis. A tetracycline concentration of 0.032 g/ml completely inhibited the appearance of inclusions in all of the 211 positive specimens. Of the positive specimens, 120 (56.9%) and 18 (8.5%) respectively showed the presence of 1 to 9 and 100 or more inclusions per microtiter well in antibiotic free medium. Other antibiotics are being tested in the same manner.A part of this paper was presented at the Canadian Public Health Association, STD meeting in Ottawa, Canada, 28–29 October 1985 相似文献
989.
Isovalerate-oxidizing strictly aneerobic bacteria were isolated from marine sediment and sewage sludge in coculture with Desulfovibrio sp. Cells stained Gram positive and behaved Gram positive also in Gram classification with KOH. Isovalerate degradation depended on interspecies hydrogen transfer to syntrophic hydrogen-oxidizing sulfate reducers or methanogens. Isovalerate was the only substrate utilized and was fermented to 3 mol acetate and 1 mol hydrogen per mol substrate. The degradation pathway was studied by enzyme assays in crude cell extracts, and included acetyl-CoA dependent activation of isovalerate, oxidation to methylcrotonyl-CoA and carboxylation to methylgluta-conyl-CoA which is hydrated and cleaved to acetoacetate and acetyl-CoA. Studies with inhibitors and ionophores suggest that energy conservation with this organism depends on either acetate efflux-driven proton symport or on an ion-gradient driven carboxylation mechanism. 相似文献
990.
Rolf Schauder Bernhard Eikmanns Rudolf K. Thauer Fritz Widdel Georg Fuchs 《Archives of microbiology》1986,145(2):162-172
In several sulfate-reducing bacteria capable of complete oxidation of acetate (or acetyl CoA), the citric acid cycle is not operative. No 2-oxoglutarate dehydrogenase activity was found in these organisms, and the labelling pattern of oxaloacetate excludes its synthesis via 2-oxo-glutarate. These sulfate-reducers contained, however, high activities of the enzymes carbon monoxide dehydrogenase and formate dehydrogenase and catalyzed an isotope exchange between CO2 and the carboxyl group of acetate (or acetyl CoA), showing a direct C-C-cleavage of activated acetic acid. These findings suggest that in the investigated sulfate-reducers acetate is oxidized to CO2 via C1 intermediates. The proposed pathway provides a possible explanation for the reported different fluoroacetate sensitivity of acetate oxidation by anaerobic bacteria, for mini-methane formation, as well as for the postulated anaerobic methane oxidation by special sulfate-reducers. 相似文献