全文获取类型
收费全文 | 443篇 |
免费 | 35篇 |
出版年
2023年 | 3篇 |
2022年 | 3篇 |
2021年 | 10篇 |
2020年 | 7篇 |
2019年 | 4篇 |
2018年 | 11篇 |
2017年 | 7篇 |
2016年 | 18篇 |
2015年 | 21篇 |
2014年 | 21篇 |
2013年 | 27篇 |
2012年 | 38篇 |
2011年 | 21篇 |
2010年 | 13篇 |
2009年 | 27篇 |
2008年 | 25篇 |
2007年 | 13篇 |
2006年 | 16篇 |
2005年 | 17篇 |
2004年 | 16篇 |
2003年 | 14篇 |
2002年 | 15篇 |
2001年 | 12篇 |
2000年 | 16篇 |
1999年 | 7篇 |
1998年 | 2篇 |
1997年 | 2篇 |
1996年 | 7篇 |
1995年 | 4篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1992年 | 4篇 |
1991年 | 3篇 |
1990年 | 4篇 |
1989年 | 12篇 |
1988年 | 5篇 |
1987年 | 4篇 |
1986年 | 5篇 |
1985年 | 8篇 |
1980年 | 2篇 |
1975年 | 2篇 |
1973年 | 2篇 |
1972年 | 2篇 |
1969年 | 2篇 |
1968年 | 7篇 |
1967年 | 4篇 |
1966年 | 2篇 |
1965年 | 2篇 |
1961年 | 1篇 |
1958年 | 1篇 |
排序方式: 共有478条查询结果,搜索用时 62 毫秒
11.
We assessed the effect of eel (Anguilla anguilla) removal from three sites of a Cantabrian stream upon its subsequent densities. In the first sample (Sept. 1986) numbers
and densities were estimated as 43, 45 and 84 ind and 3490, 3030 and 3750 ind ha −1. Removal of these eels reduced the subsequent numbers and densities which, except on two occasions, were never reached again
during the two years (eleven estimates) of study. Highest densities were recorded in the uppermost site in May and July, 1987,
coincident with a strong drought and the lowest densities occurred in 1988 during a normal wet year. We hypothesize first
that, because of a selective underground homing behaviour of eels, electro-fishing is inefficient and results in underestimates
of the population. Second, seasonal variations of water discharge and droughts may not influence the homing behaviour of'eels
until a threshold of dryness is reached. If this occurs, eels abandon their refuges and move towards the stream bottom. It
seems that in Arroyo Chabatchos this threshold was exceeded in the summer of 1987 when the highest densities were estimated.
The re-colonization of these sites experimentally depleted of eels, is a slow procces that lasts for, at least, two years. 相似文献
12.
John Soghigian Andrea Gloria‐Soria Vincent Robert Gilbert Le Goff Anna‐Bella Failloux Jeffrey R. Powell 《Molecular ecology》2020,29(19):3593-3606
Aedes aegypti is among the best‐studied mosquitoes due to its critical role as a vector of human pathogens and ease of laboratory rearing. Until now, this species was thought to have originated in continental Africa, and subsequently colonized much of the world following the establishment of global trade routes. However, populations of this mosquito on the islands in the southwestern Indian Ocean (SWIO), where the species occurs with its nearest relatives referred to as the Aegypti Group, have received little study. We re‐evaluated the evolutionary history of Ae. aegypti and these relatives, using three data sets: nucleotide sequence data, 18,489 SNPs and 12 microsatellites. We found that: (a) the Aegypti Group diverged 16 MYA (95% HPD: 7–28 MYA) from its nearest African/Asian ancestor; (b) SWIO populations of Ae. aegypti are basal to continental African populations; (c) after diverging 7 MYA (95% HPD: 4–15 MYA) from its nearest formally described relative (Ae. mascarensis), Ae. aegypti moved to continental Africa less than 85,000 years ago, where it recently (<1,000 years ago) split into two recognized subspecies Ae. aegypti formosus and a human commensal, Ae. aegypti aegypti; (d) the Madagascar samples form a clade more distant from all other Ae. aegypti than the named species Ae. mascarensis, implying that Madagascar may harbour a new cryptic species; and (e) there is evidence of introgression between Ae. mascarensis and Ae. aegypti on Réunion, and between the two subspecies elsewhere in the SWIO, a likely consequence of recent introductions of domestic Ae. aegypti aegypti from Asia. 相似文献
13.
Andrea Gloria‐Soria Sandra Y. Mendiola Valerie J. Morley Barry W. Alto Paul E. Turner 《Ecology and evolution》2020,10(12):5440-5450
It is unclear how historical adaptation versus maladaptation in a prior environment affects population evolvability in a novel habitat. Prior work showed that vesicular stomatitis virus (VSV) populations evolved at constant 37°C improved in cellular infection at both 29°C and 37°C; in contrast, those evolved under random changing temperatures between 29°C and 37°C failed to improve. Here, we tested whether prior evolution affected the rate of adaptation at the thermal‐niche edge: 40°C. After 40 virus generations in the new environment, we observed that populations historically evolved at random temperatures showed greater adaptability. Deep sequencing revealed that most of the newly evolved mutations were de novo. Also, two novel evolved mutations in the VSV glycoprotein and replicase genes tended to co‐occur in the populations previously evolved at constant 37°C, whereas this parallelism was not seen in populations with prior random temperature evolution. These results suggest that prior adaptation under constant versus random temperatures constrained the mutation landscape that could improve fitness in the novel 40°C environment, perhaps owing to differing epistatic effects of new mutations entering genetic architectures that earlier diverged. We concluded that RNA viruses maladapted to their previous environment could “leapfrog” over counterparts of higher fitness, to achieve faster adaptability in a novel environment. 相似文献
14.
15.
16.
Comment on: Atwood A, et al. Proc Natl Acad Sci U S A 2011; 108:18560-5. 相似文献
17.
Luc Friboulet Sophie Postel-Vinay Tony Sourisseau Julien Adam Annabelle Stoclin Florence Ponsonnailles Nicolas Dorvault Frédéric Commo Patrick Saulnier Sophie Salome-Desmoulez Géraldine Pottier Fabrice André Guido Kroemer Jean Charles Soria Ken André Olaussen 《Cell cycle (Georgetown, Tex.)》2013,12(20):3298-3306
ERCC1 (excision repair cross-complementation group 1) plays essential roles in the removal of DNA intrastrand crosslinks by nucleotide excision repair, and that of DNA interstrand crosslinks by the Fanconi anemia (FA) pathway and homology-directed repair processes (HDR). The function of ERCC1 thus impacts on the DNA damage response (DDR), particularly in anticancer therapy when DNA damaging agents are employed. ERCC1 expression has been proposed as a predictive biomarker of the response to platinum-based therapy. However, the assessment of ERCC1 expression in clinical samples is complicated by the existence of 4 functionally distinct protein isoforms, which differently impact on DDR. Here, we explored the functional competence of each ERCC1 protein isoform and obtained evidence that the 202 isoform is the sole one endowed with ERCC1 activity in DNA repair pathways. The ERCC1 isoform 202 interacts with RPA, XPA, and XPF, and XPF stability requires expression of the ERCC1 202 isoform (but none of the 3 others). ERCC1-deficient non-small cell lung cancer cells show abnormal mitosis, a phenotype reminiscent of the FA phenotype that can be rescued by isoform 202 only. Finally, we could not observe any dominant-negative interaction between ERCC1 isoforms. These data suggest that the selective assessment of the ERCC1 isoform 202 in clinical samples should accurately reflect the DDR-related activity of the gene and hence constitute a useful biomarker for customizing anticancer therapies. 相似文献
18.
Bernat Elvira Sabina Honisch Ahmad Almilaji Tatsiana PakladokGuilai Liu Ekaterina ShumilinaIoana Alesutan Wenting YangCarlos Munoz Florian Lang 《生物化学与生物物理学报:生物膜》2013
The Na+-coupled glucose transporter SGLT1 (SLC5A1) accomplishes concentrative cellular glucose uptake even at low extracellular glucose concentrations. The carrier is expressed in renal proximal tubules, small intestine and a variety of nonpolarized cells including several tumor cells. The present study explored whether SGLT1 activity is regulated by caveolin-1, which is known to regulate the insertion of several ion channels and carriers in the cell membrane. To this end, SGLT1 was expressed in Xenopus oocytes with or without additional expression of caveolin-1 and electrogenic glucose transport determined by dual electrode voltage clamp experiments. In SGLT1-expressing oocytes, but not in oocytes injected with water or caveolin-1 alone, the addition of glucose to the extracellular bath generated an inward current (Ig), which was increased following coexpression of caveolin-1. Kinetic analysis revealed that caveolin-1 increased maximal Ig without significantly modifying the glucose concentration required to trigger half maximal Ig (KM). According to chemiluminescence and confocal microscopy, caveolin-1 increased SGLT1 protein abundance in the cell membrane. Inhibition of SGLT1 insertion by brefeldin A (5 μM) resulted in a decline of Ig, which was similar in the absence and presence of caveolin-1. In conclusion, caveolin-1 up-regulates SGLT1 activity by increasing carrier protein abundance in the cell membrane, an effect presumably due to stimulation of carrier protein insertion into the cell membrane. 相似文献
19.
María Belén Federico María Belén Vallerga Analía Radl Natalia Soledad Paviolo José Luis Bocco Marina Di Giorgio Gastón Soria Vanesa Gottifredi 《PLoS genetics》2016,12(1)
Fanconi Anemia (FA) is a rare autosomal recessive disorder characterized by hypersensitivity to inter-strand crosslinks (ICLs). FANCD2, a central factor of the FA pathway, is essential for the repair of double strand breaks (DSBs) generated during fork collapse at ICLs. While lesions different from ICLs can also trigger fork collapse, the contribution of FANCD2 to the resolution of replication-coupled DSBs generated independently from ICLs is unknown. Intriguingly, FANCD2 is readily activated after UV irradiation, a DNA-damaging agent that generates predominantly intra-strand crosslinks but not ICLs. Hence, UV irradiation is an ideal tool to explore the contribution of FANCD2 to the DNA damage response triggered by DNA lesions other than ICL repair. Here we show that, in contrast to ICL-causing agents, UV radiation compromises cell survival independently from FANCD2. In agreement, FANCD2 depletion does not increase the amount of DSBs generated during the replication of UV-damaged DNA and is dispensable for UV-induced checkpoint activation. Remarkably however, FANCD2 protects UV-dependent, replication-coupled DSBs from aberrant processing by non-homologous end joining, preventing the accumulation of micronuclei and chromatid aberrations including non-homologous chromatid exchanges. Hence, while dispensable for cell survival, FANCD2 selectively safeguards chromosomal stability after UV-triggered replication stress. 相似文献
20.