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排序方式: 共有163条查询结果,搜索用时 15 毫秒
61.
Claudia Maria Lincoln Silva Fred Bernardes Filho Glauber Voltan Jaci Maria Santana Marcel Nani Leite Filipe Rocha Lima Luisiane de Avila Santana Natlia Aparecida de Paula Patricia Toscano Barreto Nogueira Onofre Wilson Marques-Junior Vanessa Aparecida Tomaz Carmem Sílvia Vilela Pinese Marco Andrey Cipriani Frade 《PLoS neglected tropical diseases》2021,15(8)
62.
Carmo-Silva AE Bernardes da Silva A Keys AJ Parry MA Arrabaça MC 《Photosynthesis research》2008,97(3):223-233
The C(4) photosynthetic pathway involves the assimilation of CO(2) by phosphoenolpyruvate carboxylase (PEPC) and the subsequent decarboxylation of C(4) acids. The enzymes of the CO(2) concentrating mechanism could be affected under water deficit and limit C(4) photosynthesis. Three different C(4) grasses were submitted to gradually induced drought stress conditions: Paspalum dilatatum (NADP-malic enzyme, NADP-ME), Cynodon dactylon (NAD-malic enzyme, NAD-ME) and Zoysia japonica (PEP carboxykinase, PEPCK). Moderate leaf dehydration affected the activity and regulation of PEPC in a similar manner in the three grasses but had species-specific effects on the C(4) acid decarboxylases, NADP-ME, NAD-ME and PEPCK, although changes in the C(4) enzyme activities were small. In all three species, the PEPC phosphorylation state, judged by the inhibitory effect of L: -malate on PEPC activity, increased with water deficit and could promote increased assimilation of CO(2) by the enzyme under stress conditions. Appreciable activity of PEPCK was observed in all three species suggesting that this enzyme may act as a supplementary decarboxylase to NADP-ME and NAD-ME in addition to its role in other metabolic pathways. 相似文献
63.
LM Harris L Blank RP Desai NE Welker ET Papoutsakis 《Journal of industrial microbiology & biotechnology》2001,27(5):322-328
The effect of solR inactivation on the metabolism of Clostridium acetobutylicum was examined using fermentation characterization and metabolic flux analysis. The solR-inactivated strain (SolRH) of this study had a higher rate of glucose utilization and produced higher solvent concentrations
(by 25%, 14%, and 81%, respectively, for butanol, acetone, and ethanol) compared to the wild type. Strain SolRH(pTAAD), carrying
a plasmid-encoded copy of the bifunctional alcohol/aldehyde dehydrogenase gene (aad) used in butanol production, produced even higher concentrations of solvents (by 21%, 45%, and 62%, respectively, for butanol,
acetone, and ethanol) than strain SolRH. Clarithromycin used for strain SolRH maintenance during SolRH(pTAAD) fermentations
did not alter product formation; however, tetracycline used for pTAAD maintenance resulted in 90% lower solvent production.
Journal of Industrial Microbiology & Biotechnology (2001) 27, 322–328.
Received 12 September 2000/ Accepted in revised form 21 July 2001 相似文献
64.
Sabrina L. Caetano Guilherme J. M. Rosa Rodrigo P. Savegnago Salvador B. Ramos Priscila A. Bernardes Luiz A. F. Bezerra Raysildo B. Lôbo Claudia C. P. de Paz Danísio Prado Munari 《Journal of applied genetics》2017,58(1):103-109
The aim of this study was to estimate heritability and predict breeding values for longevity among cows in herds of Nellore breed, considering the trait cow’s age at last calving (ALC), by means of survival analysis methodology. The records of 11,791 animals from 22 farms were used. The variable ALC has been used by a criterion that made it possible to include cows not only at their first calving but also at their ninth calving. The criterion used was the difference between the date of each cow’s last calving and the date of the last calving on each farm. If this difference was greater than 36 months, the cow was considered to have failed and uncensored. If not, this cow was censored, thus indicating that future calving remained possible for this cow. The survival model used for the analyses was the proportional hazards model, and the base risk was given by a Weibull distribution. The heritability estimate obtained was equal to 0.25. It was found that the ALC variable had the capacity to respond to selection for the purpose of increasing the longevity of the cows in the herds. 相似文献
65.
Diel and monthly variations in abundance, stage composition, biomass, production and feeding rates of the chaetognath Sagitta friderici were studied in the Cananéia Lagoon Estuarine System, São Paulo, Brazil. Plankton samples were collected at intervals of 4 h over several 24-h periods from February 1995 to January 1996. Sagitta friderici occurred throughout the year. Abundance of S. friderici showed considerable diel and monthly variations, ranging from 2 to 373 ind. m?3. During the low-salinity period of February and March, higher abundance was found when salinity was higher, but from May to January abundance did not show consistent pattern in relation to time of day and tidal cycle. Seasonal variation in total body length was related to temperature variations. Mean biomass ranged from 10.593 to 57.533 mgDW m?3. The daily production rate of S. friderici ranged from 0.009 to 4.488 mgC m?3 d?1. Sagitta friderici preyed mainly upon copepods (63.2%) such as Acartia (8.3%), Oncaea (7.4%) and Corycaeus (6.7%), among other prey. The daily mean feeding rate was 1.33 prey d?1 and it was related to the water temperature. 相似文献
66.
Vargas C. D. Sousa A. O. Santos C. M. Pereira A. Bernardes R. F. Rocha-Miranda C. E. Volchan E. 《Brain Cell Biology》2001,30(3):219-230
The histochemistry for the mitochondrial enzyme cytochrome oxidase (CO) was used to evaluate the levels of metabolic activity in neurons of the nucleus of the optic tract (NOT) and dorsal terminal nucleus (DTN) in the opossum (Didelphis aurita). The observations were performed in four groups: normal juveniles (4 months old), monocularly enucleated juveniles analysed when adults, normal adults (8 to 18 months old) and monocularly enucleated adults. CO labeled cells were observed to have a similar distribution along the NOT-DTN anteroposterior axis in both juvenile and adult normal animals. Monocular enucleation performed in adults produced a significant reduction of the reactive neuropil but not of the number of CO labeled cells in the deafferented NOT-DTN: the number of labeled neurons per section in the deafferented side matched those of the ipsilateral complex. In juveniles, however, this procedure caused a systematic reduction of the number of CO labeled cells in the contralateral NOT-DTN in comparison to the spared complex. The lack of reduction in the number of neurons found on the deafferented side of the NOT-DTN of monocularly enucleated adult opossums compared with the ipsilateral side might result from the presence of compensatory inputs to maintain their metabolic equivalence. However, when the monocular enucleation was performed in juvenile opossums, a statistically significant asymmetry of CO neurons in the NOT-DTN was observed. In other words, the compensatory mechanisms proposed for the adults were either absent or insufficient to achieve symmetry in juveniles, suggesting a more heavily reliance in the retinal input. 相似文献
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70.
Valentyn Oksenych Bruno Bernardes de Jesus Alexander Zhovmer Jean‐Marc Egly Frédéric Coin 《The EMBO journal》2009,28(19):2971-2980
XPB and XPD subunits of TFIIH are central genome caretakers involved in nucleotide excision repair (NER), although their respective role within this DNA repair pathway remains difficult to delineate. To obtain insight into the function of XPB and XPD, we studied cell lines expressing XPB or XPD ATPase‐deficient complexes. We show the involvement of XPB, but not XPD, in the accumulation of TFIIH to sites of DNA damage. Recruitment of TFIIH occurs independently of the helicase activity of XPB, but requires two recently identified motifs, a R‐E‐D residue loop and a Thumb‐like domain. Furthermore, we show that these motifs are specifically involved in the DNA‐induced stimulation of the ATPase activity of XPB. Together, our data demonstrate that the recruitment of TFIIH to sites of damage is an active process, under the control of the ATPase motifs of XPB and suggest that this subunit functions as an ATP‐driven hook to stabilize the binding of the TFIIH to damaged DNA. 相似文献