Studies on the existence of a pathway in liver and muscle for the conversion of glucose into glycogen without glucose 6-phosphate as an intermediate

Mixtures of (14)C-labelled glucose plus pyruvate were incubated either with rat diaphragm or slices of rat liver. Incorporation of glucose carbon into glycogen was compared with its incorporation into glucose 6-phosphate relative to the incorporation of pyruvate carbon into these metabolic products. There was no preferential incorporation of glucose carbon relative to pyruvate carbon into glycogen compared with glucose 6-phosphate in the liver slices, but there was in diaphragm. On the assumption that glucose 6-phosphate is a necessary intermediate in the conversion of pyruvate carbon into glycogen, this is evidence for the existence in muscle, but not in liver, of more than one pool of glucose 6-phosphate or of a pathway from glucose to glycogen without glucose 6-phosphate as an intermediate. Galactose carbon, relative to pyruvate carbon, was preferentially incorporated into liver glycogen, so that a substrate converted in liver into glycogen without glucose 6-phosphate as an intermediate could be detected by this approach.     

Ultrastructure of Human labial salivary glands

Summary Human labial salivary glands, obtained by biopsy from 32 subjects, were studied by light and electron microscopy. Intranuclear inclusions, unrelated to nucleoli, were present in many of the acinar nuclei in glands from 16 of the 32 donors. More than one inclusion was sometimes observed within a single nucleus. They measured about 1 in diameter, and were stainable in a variety of ways. They were eosinophilic, some were stained by Nile blue sulphate, some were PAS-positive, and all were Feulgen-negative. They were bounded by a single membrane, which never exhibited continuity with the nuclear envelope, and they showed considerable morphological variation. The more complex inclusions consisted of alternating shells of light and dark material with tiny dense granules embedded in the latter. The intranuclear inclusions, which apparently were non-viral in origin, were in some way related to the secretory cycle of the mucous cells, since they were found only in immature cells, and never in cells in which secretory products were abundant.This work was supported in part by grants from the Henry Spenadel Trust and the Max C. Fleischmann Foundation of Nevada, by grant CA-08748 from the National Cancer Institute, by grant 5 SO1 FR 05335-07 from the National Institutes of Health, by a grant from the National Cystic Fibrosis Research Foundation, and by an Institutional Grant to the School of Dental and Oral Surgery, Columbia University, from the National Institutes of Health.The authors are indebted to Dr.Louis Mandel for performing the biopsies used in this study. The expert technical assistance of Mrs.Mona Seggio is acknowledged.     

Peroxisome-desmosome complexes in mouse hepatic cells

Summary In addition to mitochondrion-desmosome complexes, peroxisome-desmosome complexes were present in mouse hepatocytes. The latter complexes consisted of a desmosome which was flanked on one or both sides by a peroxisome. Occasional desmosomes were confronted on one side by a peroxisome and on the other by a mitochondrion. It is suggested that the association between organelles and desmosomes is fortuitous, and that no functional significance can be inferred from this association.This work was supported in part by grants from the Heart Association of Northeastern Ohio, Inc., by grant 3C179 from the Cleveland Foundation, by American Cancer Society Institutional Grant In-57-H, and by grant 5 SO1 FR05335-09 from the National Institutes of Health. The expert technical assistance of Merry A. Hetrick and Jeanne Luschin is acknowledged.     

Les supports morphologiques de l'intégration dans la colonie de Veretillum cynomorium Pall. (Cnidaria,Penntularia)

The morphological supports of integration, including the nervous system, are to be found.After a morphological observation, ecto and endoderms and their intercellular junctions are presented.The regionally different mesogleal synaptic nerve nets are surrounded by ecto and endodermal nervous structures.The presence of behavioural activity centers is also discussed.

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Liste des abréviations des figures AS autozoïde sectionné - AU autozoïde - CAL canal longitudinal - CAV cavité gastrique - CC corps compact - CGL cellule globuleuse myo-épitheliale MESlame mésentériale MF microfilament - CGR cellule granuleuse myo-épithéliale - CLC cloison commune à deux autozoïdes - CLR cloison radiale - CLT cloison transversale - CM corps myélinique - CME cellule mesenchymateuse - CNE cellule nerveuse ectodermique - CNEN cellule nerveuse endodermique - CNM cellule nerveuse mésogléenne - COL colonne - CSP cellule spumeuse - CSPH cellule sphéruleuse - CU cellule urticante - D dépot de matériel intercellulaire (zonula adhaerens) - DS desmosome septé - ECT ectoderme - EE empilement d'ergastoplasme rappelant un corps de Nissl - FF fibres fasciculées h structure - G glycogène - GA granule aréolé - IC inclusion crênelée - M mitochondrie - MB membrane basale - ME mésoglée - MES lame mésentériale - MF microfilament - MT figure interprétée on terme de microtubule - MV microvilli - N noyau - E neurite - NO nodosité - P pédoncule - PC paroi des canaux - PE perforation - R rachis - SF siphonozoïde fendu - SIPH siphonozoïde - SM sipment musculaire d'une cellule myoépithéliale - T travé ous-siphonozoïdale - TME travée mésogléenne - VC vésicule claire - VME vésicule mésenchymateuse - ZI zône intermédiaire - I synapse polarisée - 2 synapse réciproque - 3 figure ressemblant à un desmosome septé - 4 contact par apposition Cc travail correspond à la première partie d'une thèse de Doctorat d'Etat, consacrée aux «Structures et aux mécanismes de l'intégration dans la colonie de Veretillum cynomoriurra» — Il a été effectué au sein de l'Equipe de Recherche Associée au C.N.R.S., n°183 (Directeur Al. Pavans de Ceccatty) avec le concours du Laboratoire Arago à Banyuls sur Mer (France) et la collaboration technique de Madame J. Villeneuve.