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901.
J. W. Ferry Slik Floris C. Breman Caroline Bernard Marloes van Beek Charles H. Cannon Karl A. O. Eichhorn Kade Sidiyasa 《Oecologia》2010,164(3):841-849
Tree species rarely exposed to burning, like in everwet tropical forests, are unlikely to be fire adapted. Therefore, one
could hypothesize that these species are affected equally by burning and that tree abundance changes are linked solely to
fire behavior. Alternatively, if species do react differentially to burning, abundance changes should be linked to tree habitat
preference and morphology. Using tree inventories from old-growth and adjacent burned Bornean forest in combination with a
database on tree morphology and habitat preference, we test these alternative hypotheses by (1) determining whether species
specific abundance changes after fire differ significantly from equal change, and (2) whether observed abundance changes are
linked to species morphology and habitat preference. We found that of 196 species tested, 125 species showed an abundance
change significantly different from that expected under our null model of equal change. These abundance changes were significantly
linked to both tree morphology and habitat preference. Abundance declines were associated with slope or ridge preference,
thin barks, and limited seed dormancy. Abundance increases were associated with high light preference, small adult stature,
light wood, large leaves, small seeds and long seed dormancy. While species habitat preference and morphology explained observed
abundance increases well, abundance declines were only weakly associated with them (R
2 ~ 0.09). This suggests that most tree mortality was random and everwet tropical tree species are poorly fire adapted. As
fire frequencies are increasing in the everwet tropics, this might eventually result in permanently altered species compositions
and even species extinctions. 相似文献
902.
Mariana Royer Gaëtan Herbette Véronique Eparvier Jacques Beauchêne Bernard Thibaut Didier Stien 《Phytochemistry》2010,71(14-15):1708-1713
In order to explain the durability of the Moraceae plant family, phytochemistry of Bagassa guianensis was performed. Ethyl acetate extract was obtained from the heartwood and 18 secondary metabolites were isolated, including 6 moracins [6-O-methyl-moracin M, 6-O-methyl-moracin N and moracin Z; previously identified: moracin M, moracin N and moracin P], 8 stilbenoids [presently identified: (?)-epialboctalol and arachidin 4; previously identified: alboctalol, trans-resveratrol, arachidin 2, trans-oxyresveratrol and artogomezianol], 3 previously identified flavonoids, steppogenin, katuranin and dihydromorin, β-sitosterol and resorcinol. Previous studies suggest that stilbenoids are responsible for the natural durability of wood. Our study has determined that B. guianensis is closely related to Morus sp. in phylogeny and should be included in the Moreae sensu stricto tribe of the Moraceae family. 相似文献
903.
Steven Maenhout Bernard De Baets Geert Haesaert 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,120(2):415-427
Accurate prediction of the phenotypic performance of a hybrid plant based on the molecular fingerprints of its parents should
lead to a more cost-effective breeding programme as it allows to reduce the number of expensive field evaluations. The construction
of a reliable prediction model requires a representative sample of hybrids for which both molecular and phenotypic information
are accessible. This phenotypic information is usually readily available as typical breeding programmes test numerous new
hybrids in multi-location field trials on a yearly basis. Earlier studies indicated that a linear mixed model analysis of
this typically unbalanced phenotypic data allows to construct ɛ-insensitive support vector machine regression and best linear
prediction models for predicting the performance of single-cross maize hybrids. We compare these prediction methods using
different subsets of the phenotypic and marker data of a commercial maize breeding programme and evaluate the resulting prediction
accuracies by means of a specifically designed field experiment. This balanced field trial allows to assess the reliability
of the cross-validation prediction accuracies reported here and in earlier studies. The limits of the predictive capabilities
of both prediction methods are further examined by reducing the number of training hybrids and the size of the molecular fingerprints.
The results indicate a considerable discrepancy between prediction accuracies obtained by cross-validation procedures and
those obtained by correlating the predictions with the results of a validation field trial. The prediction accuracy of best
linear prediction was less sensitive to a reduction of the number of training examples compared with that of support vector
machine regression. The latter was, however, better at predicting hybrid performance when the size of the molecular fingerprints
was reduced, especially if the initial set of markers had a low information content. 相似文献
904.
Induced plant resistance (IR) against pathogen infection can be triggered by various chemical and biological elicitors. The effectiveness of elicitors to induce resistance as a practical means to control plant disease makes use of the plant’s own defence mechanisms triggered by resistance inducing agents. The aim of the present study was to examine the possible side effects of IR on the establishment and the efficiency of a rhizobial symbioses (Bradyrhizobium japonicum-soybean) and an arbuscular mycorrhizal symbioses (Glomus mosseae —soybean). IR was triggered by applying, acibenzolar S-methyl (ASM) at 80 mg a.i. L?1 by two ways: seed soaking or foliar spray. Chitinase activity, used as a biochemical marker of IR, increased when ASM was applied both as seed soaking or as foliar spray. In vitro ASM showed no direct effect on the growth of B. japonicum and induced, only at a high concentration, a slight inhibition effect on spore germination of G. mosseae. ASM caused after both treatments a significant decrease in the number of nodules. Nitrogen content in aerial parts and roots decreased. On the other hand, ASM showed no significant effect on the frequency of colonization by G.mosseae but reduced the intensity of colonization and the proportion of arbuscules. The possible interaction between IR and the induction and suppression of defence-like mechanisms during symbiotic processes is discussed. 相似文献
905.
Chanyarat Paungfoo-Lonhienne Thierry G.A. Lonhienne Stephen R. Mudge Peer M. Schenk Michael Christie Bernard J. Carroll Susanne Schmidt 《Plant physiology》2010,153(2):799-805
Phosphorus (P) enters roots as inorganic phosphate (Pi) derived from organic and inorganic P compounds in the soil. Nucleic acids can support plant growth as the sole source of P in axenic culture but are thought to be converted into Pi by plant-derived nucleases and phosphatases prior to uptake. Here, we show that a nuclease-resistant analog of DNA is taken up by plant cells. Fluorescently labeled S-DNA of 25 bp, which is protected against enzymatic breakdown by its phosphorothioate backbone, was taken up and detected in root cells including root hairs and pollen tubes. These results indicate that current views of plant P acquisition may have to be revised to include uptake of DNA into cells. We further show that addition of DNA to Pi-containing growth medium enhanced the growth of lateral roots and root hairs even though plants were P replete and had similar biomass as plants supplied with Pi only. Exogenously supplied DNA increased length growth of pollen tubes, which were studied because they have similar elongated and polarized growth as root hairs. Our results indicate that DNA is not only taken up and used as a P source by plants, but ironically and independent of Pi supply, DNA also induces morphological changes in roots similar to those observed with P limitation. This study provides, to our knowledge, first evidence that exogenous DNA could act nonspecifically as signaling molecules for root development.Phosphorus (P) is an essential macronutrient that limits plant growth in many situations due to a low availability in soils (for review, see Schachtman et al., 1998; Raghothama, 1999; Vance et al., 2003; Lambers et al., 2008). P enters plant roots as orthophosphates (Pi) via active transport across the plasma membrane (Smith et al., 2003; Park et al., 2007; Xu et al., 2007). Concentrations of Pi in soil solution are generally very low (<10 μm; Bieleski, 1973) and plants have evolved root specializations to access P from inorganic and organic sources (Raghothama, 1999; Hinsinger, 2001; López-Bucio et al., 2003; Vance et al., 2003; Lambers et al., 2008). Roots exude enzymes and chemicals to mobilize P directly from soil compounds or indirectly via enhanced activity of soil microbes, and form symbioses with P-mobilizing mycorrhizal fungi (Schachtman et al., 1998; Raghothama, 1999; Bucher, 2007).However, similar to other nutrients, notably nitrogen, research on P nutrition of plants has focused on inorganic sources although organic P (Porg) in soil can account for 40% to 80% of the total P pool of mineral and organic soils, respectively (Bower, 1945; Raghothama, 1999; Vance et al., 2003). Porg compounds in soils are derived from plant residues, soil biota, and from synthesis by soil microbes (Jencks et al., 1964). Soil Porg is composed primarily of phospholipids, nucleic acids, and phytin (Dyer and Wrenshall, 1941). Phytic acid (inositol hexaphosphate) and its salts phytate, account for a large proportion of the Porg pool of soils (Anderson, 1980). Nucleic acids (RNA, DNA) represent approximately 1% to 2% of the soil Porg pool (Dalal, 1977). It can be released from prokaryotic and eukaryotic cells after death and protected against nuclease degradation by its adsorption on soil colloids and sand particles (Pietramellara et al., 2009).Although Porg can be a substantial constituent of the soil P pool, its contribution to the P nutrition of plants is poorly understood. Porg can be converted to Pi via root-exuded enzymes (Tarafdar and Claassen, 1988; Marschner, 1995; Vance et al., 2003). Secretion of nucleolytic enzymes and breakdown of nucleic acid were considered the reason for the observed growth of axenic Arabidopsis (Arabidopsis thaliana) and wheat (Triticum aestivum) on nucleic acid substrates as the sole P source (Chen et al., 2000; Richardson et al., 2000).Whether plants take up intact DNA has not been reported. We recently showed that roots take up protein, possibly via endocytosis (Paungfoo-Lonhienne et al., 2008). We hypothesized that roots may take up DNA by a similar process and grew Arabidopsis in the presence of phosphorothioate oligonucleotides (S-DNA) labeled with Cy3-fluorescent dye. S-DNA has a sulfur backbone and cannot be digested by plant nucleases, allowing tracking DNA of known size into cells (Spitzer and Eckstein, 1988). We examined if S-DNA of 25 nucleotides in length enters root hairs and pollen tubes as both types of cells are strongly elongated and have similar polarized growth (Schiefelbein et al., 1993; Hepler et al., 2001). We also assessed if addition of DNA to the growth medium affects the morphology of roots and pollen tubes. Here, we present evidence that plants take up DNA and demonstrate that the presence of DNA in the growth medium enhances lateral branching of roots, and the length of root hairs and pollen tubes, irrespective of Pi supply. 相似文献
906.
Yuan-Ying Peng Yu-Ming Wei Bernard R. Baum Ze-Hong Yan Xiu-Jin Lan Shou-Fen Dai You-Liang Zheng 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(5):985-1000
The development and application of molecular methods in oats has been relatively slow compared with other crops. Results from
the previous analyses have left many questions concerning species evolutionary relationships unanswered, especially regarding
the origins of the B and D genomes, which are only known to be present in polyploid oat species. To investigate the species
and genome relationships in genus Avena, among 13 diploid (A and C genomes), we used the second intron of the nuclear gene FLORICAULA/LEAFY (FL int2) in seven tetraploid (AB and AC genomes), and five hexaploid (ACD genome) species. The Avena FL int2 is rather long, and high levels of variation in length and sequence composition were found. Evidence for more than one copy
of the FL int2 sequence was obtained for both the A and C genome groups, and the degree of divergence of the A genome copies was greater
than that observed within the C genome sequences. Phylogenetic analysis of the FL int2 sequences resulted in topologies that contained four major groups; these groups reemphasize the major genomic divergence
between the A and C genomes, and the close relationship among the A, B, and D genomes. However, the D genome in hexaploids
more likely originated from a C genome diploid rather than the generally believed A genome, and the C genome diploid A. clauda may have played an important role in the origination of both the C and D genome in polyploids. 相似文献
907.
Valeria Mozzetti Franck Grattepanche Déborah Moine Bernard Berger Enea Rezzonico Leo Meile Fabrizio Arigoni Christophe Lacroix 《Microbial cell factories》2010,9(1):60
Background
Oxidative stress can severely compromise viability of bifidobacteria. Exposure of Bifidobacterium cells to oxygen causes accumulation of reactive oxygen species, mainly hydrogen peroxide, leading to cell death. In this study, we tested the suitability of continuous culture under increasing selective pressure combined with immobilized cell technology for the selection of hydrogen peroxide adapted Bifidobacterium cells. Cells of B. longum NCC2705 were immobilized in gellan-xanthan gum gel beads and used to continuously ferment MRS medium containing increasing concentration of H2O2 from 0 to 130 ppm. 相似文献908.
Diap Graciela Amuasi John Boakye Isaac Sevcsik Ann-Marie Pecoul Bernard 《Malaria journal》2010,9(1):1-11
Background
Malaria microscopy and rapid diagnostic tests are insensitive for very low-density parasitaemia. This insensitivity may lead to missed asymptomatic sub-microscopic parasitaemia, a potential reservoir for infection. Similarly, mixed infections and interactions between Plasmodium species may be missed. The objectives were first to develop a rapid and sensitive PCR-based diagnostic method to detect low parasitaemia and mixed infections, and then to investigate the epidemiological importance of sub-microscopic and mixed infections in Rattanakiri Province, Cambodia.Methods
A new malaria diagnostic method, using restriction fragment length polymorphism analysis of the cytochrome b genes of the four human Plasmodium species and denaturing high performance liquid chromatography, has been developed. The results of this RFLP-dHPLC method have been compared to 1) traditional nested PCR amplification of the 18S rRNA gene, 2) sequencing of the amplified fragments of the cytochrome b gene and 3) microscopy. Blood spots on filter paper and Giemsa-stained blood thick smears collected in 2001 from 1,356 inhabitants of eight villages of Rattanakiri Province have been analysed by the RFLP-dHPLC method and microscopy to assess the prevalence of sub-microscopic and mixed infections.Results
The sensitivity and specificity of the new RFLP-dHPLC was similar to that of the other molecular methods. The RFLP-dHPLC method was more sensitive and specific than microscopy, particularly for detecting low-level parasitaemia and mixed infections. In Rattanakiri Province, the prevalences of Plasmodium falciparum and Plasmodium vivax were approximately two-fold and three-fold higher, respectively, by RFLP-dHPLC (59% and 15%, respectively) than by microscopy (28% and 5%, respectively). In addition, Plasmodium ovale and Plasmodium malariae were never detected by microscopy, while they were detected by RFLP-dHPLC, in 11.2% and 1.3% of the blood samples, respectively. Moreover, the proportion of mixed infections detected by RFLP-dHPLC was higher (23%) than with microscopy (8%).Conclusions
The rapid and sensitive molecular diagnosis method developed here could be considered for mass screening and ACT treatment of inhabitants of low-endemicity areas of Southeast Asia. 相似文献909.
Tiziana Gigliobianco Bernard Lakaye Pierre Wins Benaïssa El Moualij Willy Zorzi Lucien Bettendorff 《BMC microbiology》2010,10(1):148
Background
E. coli cells are rich in thiamine, most of it in the form of the cofactor thiamine diphosphate (ThDP). Free ThDP is the precursor for two triphosphorylated derivatives, thiamine triphosphate (ThTP) and the newly discovered adenosine thiamine triphosphate (AThTP). While, ThTP accumulation requires oxidation of a carbon source, AThTP slowly accumulates in response to carbon starvation, reaching ~15% of total thiamine. Here, we address the question whether AThTP accumulation in E. coli is triggered by the absence of a carbon source in the medium, the resulting drop in energy charge or other forms of metabolic stress. 相似文献910.
Rune Jacobsen Torben Martinussen Lene Christiansen Bernard Jeune Karen Andersen‐Ranberg James W. Vaupel Kaare Christensen 《Aging cell》2010,9(6):1004-1009
Studies of Nordic twins suggest an increased genetic influence on mortality with age. Contrary to this, the heterogeneity hypothesis predicts that the mortality of individuals carrying a ‘frail’ or ‘risky’ genotype in a population will approach that of noncarriers with age because of selection pressure. The ApoE ε4 allele is associated with an increased mortality risk, and its effect has been suggested to decrease with age. Here, we investigated the effect of ApoE ε4 allele on survival in a sample of the healthiest and long‐lived Danes. The study population comprised Danes born in 1905 and a replicate sample of the 1895 cohort. For the 1905 cohort, a total of 350 carriers and 1256 noncarriers of the ApoE ε4 allele were followed from 1998 until death or end of follow‐up. Cox regression models were used for the analysis. Of the 1606 persons with known ApoE ε4 status in 1998, 1546 had died at the end of the 10‐year follow‐up. Carriers of the ApoE ε4 allele had an increased mortality compared to noncarriers, and the influence of ApoE status on mortality increased in the age interval 92–103. For the covariates sex and independency status, the difference in relative risk of death between groups decreased with advancing age. Our findings of increasing influence of ApoE ε4 allele on mortality with age do not support previous findings of decreased influence ApoE ε4 allele on mortality with age, and alternative models such as the multifactorial threshold models should be considered for understanding the genetic effects on mortality at advanced age. 相似文献