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71.
Comparison of Enrichment Procedures for Fluorescent Antibody and Cultural Detection of Salmonellae in Raw Meat and Poultry 总被引:2,自引:2,他引:0 下载免费PDF全文
No advantage was shown in preenriching raw meat samples for detecting salmonellae by fluorescent antibodies or culture. Trypticase soy-tryptose (Edwards and Ewing, 1972) was equal to or better than selenite-cystine as a postenrichment broth. 相似文献
72.
73.
Further Evaluation of Immunofluorescence Techniques for Detection of Shigellae in Fecal Specimens 总被引:1,自引:0,他引:1 下载免费PDF全文
The fluorescent antibody (FA) tests for group B and group D Shigella were reevaluated. Duplicate swab specimens from patients suspected of having shigellosis were cultured shortly after collection and after transport in a soft-agar holding medium. Smears for FA examination were made at the same time. Results obtained for the group D Shigella agree closely with those obtained in previous studies. The percentage of isolations of group B Shigella from transported specimens which were positive by FA was 59.6% as compared to 39.3 and 53.3% in previous studies. S. flexneri was isolated from 76.7% of the FA-positive specimens when they were examined shortly after collection. More isolates of Shigella were obtained when specimens were examined by both methods than when either method was used alone. The results indicated that FA tests for both group B and group D Shigella are practical and may be useful to laboratories engaged in Shigella isolation. 相似文献
74.
Rivero-Cruz Isabel Cristians Sol Ovalle-Magallanes Berenice Mata Rachel 《Phytochemistry Reviews》2019,18(6):1435-1455
Phytochemistry Reviews - The term copalchi has been used in the scientific literature to define a group of plants of the Rubiaceae and Euphorbiaceae families with extremely bitter stem-barks,... 相似文献
75.
76.
Evaluation of Frozen Fixed Smears for Use in Fluorescent Antibody Studies of Salmonellae 总被引:1,自引:1,他引:0 下载免费PDF全文
Berenice M. Thomason 《Applied microbiology》1974,27(2):418-419
Smears of broth cultures of 28 Salmonella serotypes were fixed with Kirkpatrick fixative and stored at -20 C. Results indicate that organisms retain the ability to stain at maximal fluorescence intensity for as long as 2 years. 相似文献
77.
Berenice Beaumont 《BMJ (Clinical research ed.)》1978,1(6110):445-446
78.
Kate E. Best Marie‐Claude Addor Larraitz Arriola Eszter Balku Ingeborg Barisic Fabrizio Bianchi Elisa Calzolari Rhonda Curran Berenice Doray Elizabeth Draper Ester Garne Miriam Gatt Martin Haeusler Jorieke Bergman Babak Khoshnood Kari Klungsoyr Carmen Martos Anna Materna‐Kiryluk Carlos Matias Dias Bob McDonnell Carmel Mullaney Vera Nelen Mary O'Mahony Annette Queisser‐Luft Hanitra Randrianaivo Anke Rissmann Catherine Rounding Antonin Sipek Rosie Thompson David Tucker Diana Wellesley Natalya Zymak‐Zakutnia Judith Rankin 《Birth defects research. Part A, Clinical and molecular teratology》2014,100(9):695-702
Background: Hirschsprung's disease is a congenital gut motility disorder, characterised by the absence of the enteric ganglion cells along the distal gut. The aim of this study was to describe the epidemiology of Hirschsprung's disease, including additional congenital anomalies, total prevalence, trends, and association with maternal age. Methods: Cases of Hirschsprung's disease delivered during 1980 to 2009 notified to 31 European Surveillance of Congenital Anomaly registers formed the population‐based case‐series. Prevalence rates and 95% confidence intervals were calculated as the number of cases per 10,000 births. Multilevel Poisson regression was performed to investigate trends in prevalence, geographical variation and the association with maternal age. Results: There were 1,322 cases of Hirschsprung's disease among 12,146,210 births. The total prevalence was 1.09 (95% confidence interval, 1.03–1.15) per 10,000 births and there was a small but significant increase in prevalence over time (relative risk = 1.01; 95% credible interval, 1.00–1.02; p = 0.004). There was evidence of geographical heterogeneity in prevalence (p < 0.001). Excluding 146 (11.0%) cases with chromosomal anomalies or genetic syndromes, there were 1,176 cases (prevalence = 0.97; 95% confidence interval, 0.91–1.03 per 10,000 births), of which 137 (11.6%) had major structural anomalies. There was no evidence of a significant increased risk of Hirschsprung's disease in cases born to women aged ≥35 years compared with those aged 25 to 29 (relative risk = 1.09; 95% credible interval, 0.91–1.31; p = 0.355). Conclusion: This large population‐based study found evidence of a small increasing trend in Hirschsprung's disease and differences in prevalence by geographic location. There was also no evidence of an association with maternal age. Birth Defects Research (Part A), 100:695–702, 2014. © 2014 Wiley Periodicals, Inc. 相似文献
79.
Berenice Palacios-González Angel Zarain-Herzberg Isabel Flores-Galicia Lilia G. Noriega Gabriela Alemán-Escondrillas Teresa Zariñan Alfredo Ulloa-Aguirre Nimbe Torres Armando R. Tovar 《Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids》2014,1841(1):132-140
Obesity is a public health problem that contributes to the development of insulin resistance, which is associated with an excessive accumulation of lipids in skeletal muscle tissue. There is evidence that soy protein can decrease the ectopic accumulation of lipids and improves insulin sensitivity; however, it is unknown whether soy isoflavones, particularly genistein, can stimulate fatty acid oxidation in the skeletal muscle. Thus, we studied the mechanism by which genistein stimulates fatty acid oxidation in the skeletal muscle. We showed that genistein induced the expression of genes of fatty acid oxidation in the skeletal muscle of Zucker fa/fa rats and in leptin receptor (ObR)-silenced C2C12 myotubes through AMPK phosphorylation. Furthermore, the genistein-mediated AMPK phosphorylation occurred via JAK2, which was possibly activated through a mechanism that involved cAMP. Additionally, the genistein-mediated induction of fatty acid oxidation genes involved PGC1α and PPARδ. As a result, we observed that genistein increased fatty acid oxidation in both the control and silenced C2C12 myotubes, as well as a decrease in the RER in mice, suggesting that genistein can be used in strategies to decrease lipid accumulation in the skeletal muscle. 相似文献
80.
Jéssie Haigert Sudati Roselei Fachinetto Romaiana Picada Pereira Aline Augusti Boligon Margareth Linde Athayde Felix Antunes Soares Nilda Berenice de Vargas Barbosa João Batista Teixeira Rocha 《Neurochemical research》2009,34(8):1372-1379
Valeriana officinalis L. (Valerian) is widely used as a traditional medicine to improve the quality of sleep. Although V. officinalis have been well documented as promising pharmacological agent; the exact mechanisms by which this plant act is still unknown.
Limited literature data have indicated that V. officinalis extracts can exhibit antioxidant properties against iron in hippocampal neurons in vitro. However, there is no data available
about the possible antioxidant effect of V. officinalis against other pro-oxidants in brain. In the present study, the protective effect of V. officinalis on lipid peroxidation (LPO) induced by different pro-oxidant agents with neuropathological importance was examined. Ethanolic
extract of valerian (0–60 μg/ml) was tested against quinolinic acid (QA); 3-nitropropionic acid; sodium nitroprusside; iron
sulfate (FeSO4) and Fe2+/EDTA induced LPO in rat brain homogenates. The effect of V. officinalis in deoxyribose degradation and reactive oxygen species (ROS) production was also investigated. In brain homogenates, V. officinalis inhibited thiobarbituric acid reactive substances induced by all pro-oxidants tested in a concentration dependent manner.
Similarly, V. officinalis caused a significant decrease on the LPO in cerebral cortex and in deoxyribose degradation. QA-induced ROS production in
cortical slices was also significantly reduced by V. officinalis. Our results suggest that V. officinalis extract was effective in modulating LPO induced by different pro-oxidant agents. These data may imply that V. officinalis extract, functioning as antioxidant agent, can be beneficial for reducing insomnia complications linked to oxidative stress. 相似文献