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Khan NA Githiri SM Benitez ER Abe J Kawasaki S Hayashi T Takahashi R 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,117(4):479-487
Early-maturing cultivars of soybean [Glycine max (L.) Merr.] native to the shores of the Sea of Okhotsk (Sakhalin and Kuril Islands) and eastern Hokkaido (northern Japan) have a strong tendency to produce cleistogamous flowers throughout their blooming period. A previous study revealed that cleistogamy is controlled by a minimum of two genes with epistatic interaction, one of which is associated with a maturity gene responsible for insensitivity to incandescent long daylength (ILD). This study was conducted to determine the genetic basis of cleistogamy in more detail by QTL mapping. F(2) to F(4) progenies derived from a cross between a cleistogamous cv. Karafuto-1 and a chasmogamous cv. Toyosuzu were used. A molecular linkage map spanning 2,180 cM comprising 500 markers was constructed using 89 F(2) plants. The markers were distributed in 25 linkage groups. An interval mapping method to analyze categorical traits identified four QTLs for cleistogamy, cl1, cl2, cl3 and cl4, in molecular linkage groups (MLGs) C2, D1a, I and L, respectively. Alleles derived from Karafuto-1 had additive effects to increase probability of cleistogamy at cl3 and cl4, whereas the alleles had additive effects to decrease the probablity at cl1 and cl2. Progeny test confirmed the effects of cl3, which had the highest LOD score (5.20). Composite interval mapping revealed four QTLs for flowering date, fd5-fd8. Judging from relative location with markers and association with ILD responses, fd7 and fd8 may correspond to maturity genes E4 and E3, respectively. cl3 and cl4 were located at similar positions as fd7 and fd8, suggesting that the two maturity genes may control cleistogamy by either pleiotropy or close linkage. 相似文献
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F. J. Benitez J. Beltran-Heredia F. J. Real J. L. Acero 《Bioprocess and biosystems engineering》1999,21(5):459-464
The decomposition of the organic substrate present in wine distillery wastewaters (WDW) is studied in batch reactors, by an ozonation process, by an aerobic degradation and by another ozonation of the aerobically pretreated wastewaters. In the ozonation process, the effects on the substrate removal obtained of the temperature, pH and the presence of H2O2 and UV radiation are established, and an approximate kinetic study is conducted which leads to the evaluation of the apparent kinetic constants for the substrate reduction. In the aerobic degradation treatment, the evolution of the substrate, biomass and total phenolic compounds are followed during the process, and a kinetic study is performed by using the Contois model, which applied to the experimental data provides the specific kinetic parameters qmax and K1. Finally, in the ozonation of the pretreated wastewaters, the?influence of the operating variables is established, and the effect of this aerobic pretreatment on the substrate removal and kinetic constants obtained in the ozonation stage is also discussed. 相似文献
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F. J. Benitez J. Beltran-Heredia J. Torregrosa J. R. Dominguez 《Bioprocess and biosystems engineering》1999,21(4):355-361
The reduction of the pollutant organic matter present in wastewaters generated in the black olive production process is studied by an aerobic degradation, and by the combination of two successive steps: an ozonation pretreatment followed by an aerobic degradation. In the single aerobic process, in addition to the biomass evolution which is followed during each experiment, the removal of the pollutant load is evaluated by means of global parameters which are directly related with the organic matter, as the chemical oxygen demand and the total phenolic compounds content. A kinetic study is performed by using the Monod model, which applied to the experimental data, provides the specific kinetic parameters of this model: the kinetic constant for the substrate decomposition rate, the cellular yield coefficient and the kinetic constant for the biomass decrease during the death phase of microorganisms. In the combined process, an ozonation pretreatment is conducted with experiments where the ozone partial pressure is varied, and an important reduction in the phenolic compounds is achieved. The kinetic parameters of the following aerobic degradation stage are also evaluated, and the effect of the chemical oxidation pretreatment on this biological stage is discussed. 相似文献
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SELECTIVE DEPOSITION OF LANTHANUM IN MAMMALIAN CARDIAC CELL MEMBRANES : Ultrastructural and Electrophysiological Evidence 总被引:7,自引:3,他引:4 下载免费PDF全文
Perfusion of beating false tendons of the dog heart with ionic lanthanum produced drastic but reversible modifications of the excitability and the transmembrane action potential of Purkinje cells. Ultrastructural examination of these cells revealed the appearance of a fine extracellular precipitate detectable on unstained sections. In addition, specimens perfused with La+++ showed a striking increase in the contrast of the sarcolemma, particularly in gap junctions and in pinocytic vesicles. La+++ deposits were restricted to the cytoplasmic leaflets of the sarcolemma; no precipitates were found at the plasma membrane of fibroblasts, endothelial and smooth muscle cells, or unmyelinated nerve fibers present in the same specimens. A selective deposition of La+++ was also observed in the sarcolemma of atrial and ventricular cells of dog, rabbit, and cat hearts, as well as in the membrane of the transverse tubular system of ventricular cells. Both the electrophysiological effects and the ultrastructural membrane deposits produced by La+++ disappeared when the specimens were subsequently perfused with phosphate-containing tyrode solution. These results tend to demonstrate that a distinctive feature of the sarcolemma of mammalian cardiac cells is the presence of regions with a high surface density of binding sites for polyvalent cations. 相似文献
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David Gendron Anne-Marie Lemay Claudine Tremblay Laetitia JA Lai Anick Langlois émilie Bernatchez Nicolas Flamand Marie-Renée Blanchet Anthony S Don Ynuk Bossé élyse Bissonnette David Marsolais 《Respiratory research》2015,16(1)