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81.
Plant-based host preference and larval competition of the tachinid parasitoid Epicampocera succincta
We investigated the causes of among-plant variation in the parasitism rate of Pieris melete larvae (Lepidoptera: Pieridae) by the parasitoid fly Epicampocera succincta (Diptera: Tachinidae). The rate of parasitism by E. succincta was much higher on the bitter cress Cardamine appendiculata than on any other cruciferous plants. Adult female flies were found to be more attracted to C. appendiculata than to other cruciferous plants for searching for hosts. The parasitoid appeared to be unable to distinguish P. melete from an alternative host, Pieris rapae larvae, that coexisted with P. melete on most crucifer plants. Similarly, E. succincta failed to avoid P. rapae parasitized by the braconid wasp Cotesia glomerata. C. glomerata is a superior competitor to E. succincta if occupying the same host, killing the host before the E. succincta larva can grow and depriving the larva of the chance to survive. E. succincta attacked P. rapae larvae on most cruciferous plants, many of which were already occupied by C. glomerata; only on Cardamine appendiculata was E. succincta free from interspecific competition, because only P. melete was found on this particular plant. However, the strong preference for the hosts on C. appendiculata incurred heavy intraspecific competition among larvae on this plant, killing as great a proportion of larvae as interspecific
competition did on the other plants. The balance between the strength of intraspecific competition on the preferred plant
and of interspecific competition on the other plants appeared to maintain plant preference by E. succincta.
Received: November 13, 2000 / Accepted: April 27, 2001 相似文献
82.
H. Ohsaki R. Haba T. Matsunaga M. Nakamura H. Kiyomoto E. Hirakawa 《Cytopathology》2009,20(4):224-230
Objective: Cannibalism of one cell by another in voided urine cytology has been considered a cytological feature for differentiating urothelial carcinoma (UC) from benign lesions. Recently, however, we observed cannibalism in voided urine obtained from patients with renal glomerular disease (RGD). The purpose of this study was to determine the cytomorphological and immunocytochemical characteristics of cannibalism in voided urine from RGD. Methods: Seventy cytology specimens of voided urine were examined and the findings were compared with the histological findings. In addition, we compared the cytomorphological and immunocytochemical differences in cannibalism found in RGD and cases of UC selected as showing cannabilism. Results: Cannibalism in voided urine was found in three (5.5%) of 55 RGD cases. The finding was measured as (1+) < 5 cells, (2+) 5–20 cells, and (3+) > 20 cells and was (1+) in all three RGD cases, compared with 6.7%, 60% and 33.3% respectively in 15 UC cases. Differences in low cellularity cases (1+) and moderate to high cellularity cases (2+ or 3+) were statistically significant between RGD (3 and 0) and UC (1 and 14) (P=0.005). The maximum diameter of cannibalized cells in RGD was 24.3–33.0 μm (mean 29.8 μm) versus 18.0–30.4 μm (mean 23.3 μm) in UC (P=0.004). Necrosis and isomorphic erythrocytes were absent in RGD, but were found in 46.7% and 86.7%, respectively, of UC cases (P=0.245 and P=0.012). Dysmorphic erythrocytes were identified in all three cases with RGD and 13.3% of UC (P=0.012). Vimentin reactivity was found in all cases with cannibalism in RGD, but never in UC (P=0.001). Conclusions: Our results demonstrated that cannibalism in voided urine is present not only in UC but also in RGD. Furthermore, we showed that cellularity of cannibalism, vimentin reactivity and background differed significantly and can be used for differential diagnosis between the two groups. 相似文献
83.
The effects of cisplatin on the cell cycle and DNA synthesis of human lung adenocarcinoma cell line PC-9 were examined by flow cytometry. The cellular DNA content and the bromodeoxyuridine (BrdUrd) incorporation rate were measured simultaneously using a monoclonal anti-BrdUrd antibody. Following exposure to cisplatin (1.0 micrograms/ml) for 1 and 24 hr, the bivariate DNA/BrdUrd distributions revealed a delayed S-phase transit and an accumulation of cells in the G2M phase. The BrdUrd-linked green fluorescence intensity continued to decrease with the lapse of time. However, early- and mid-S-phase cells soon recovered DNA synthesis activity, and the former showed higher activity than the control cells. These findings suggested the vigorous DNA synthesis of cells in early S phase. However, for quantitative analysis of chemotherapeutic effects, some problems remained to be resolved regarding the condition for DNA denaturation and its alteration by the agents. 相似文献
84.
The adaptations of young insect larvae to factors causing mortality may not depend on whole-body size but may instead depend
substantially on the size of specific body parts. Using two closely related plant species, Arabis flagellosa, which has leaves that are tougher and difficult to digest, and A. gemmifera, which has leaves that are softer and difficult to convert, we showed that larvae of the butterfly Pieris napi exhibit specific adaptations through changes in body-part size based on different traits of their host plants. For a given
egg size, the head widths of hatchlings from eggs collected from A. flagellosa were significantly larger than those of hatchlings from eggs collected from A. gemmifera. In addition, larger heads were accompanied by smaller abdomens in hatchlings originating from A. flagellosa, whereas the opposite pattern was observed in hatchlings from A. gemmifera. The time to completion of the first feeding on leaves of A. gemmifera was not affected by either egg or head size regardless of the original food plant. However, the time to completion of the
first feeding on A. flagellosa decreased with increasing head size of hatchlings, regardless of the original food plant. Furthermore, even though egg sizes
did not differ between treatments, larvae originating from A. gemmifera and A. flagellosa exhibited similar weight gain on leaves of A. flagellosa, whereas larvae originating from A. gemmifera gained more weight than larvae from A. flagellosa when reared on leaves of A. gemmifera. These results suggest that selection in young larvae for adaptations to environmental conditions may operate on specific
larval body-part sizes. 相似文献
85.
Suzuki M Otsuka T Ohsaki Y Cheng J Taniguchi T Hashimoto H Taniguchi H Fujimoto T 《Molecular biology of the cell》2012,23(5):800-810
Apolipoprotein B-100 (ApoB) is the principal component of very low density lipoprotein. Poorly lipidated nascent ApoB is extracted from the Sec61 translocon and degraded by proteasomes. ApoB lipidated in the endoplasmic reticulum (ER) lumen is also subjected to proteasomal degradation, but where and how it dislocates to the cytoplasm remain unknown. In the present study, we demonstrate that ApoB after lipidation is dislocated to the cytoplasmic surface of lipid droplets (LDs) and accumulates as ubiquitinated ApoB in Huh7 cells. Depletion of UBXD8, which is almost confined to LDs in this cell type, decreases recruitment of p97 to LDs and causes an increase of both ubiquitinated ApoB on the LD surface and lipidated ApoB in the ER lumen. In contrast, abrogation of Derlin-1 function induces an accumulation of lipidated ApoB in the ER lumen but does not increase ubiquitinated ApoB on the LD surface. UBXD8 and Derlin-1 bind with each other and with lipidated ApoB and show colocalization around LDs. These results indicate that ApoB after lipidation is dislocated from the ER lumen to the LD surface for proteasomal degradation and that Derlin-1 and UBXD8 are engaged in the predislocation and postdislocation steps, respectively. 相似文献
86.
Nagano H Hanai R Yamada H Matsushima M Miura Y Hoya T Ozawa M Fujiwara M Kodama H Torihata A Onuki H Nezu Y Kawai S Yamazaki M Hirota H Saito Y Tori M Ohsaki A Gong X Kuroda C 《Chemistry & biodiversity》2012,9(4):789-805
The chemical constituents of the root extracts and the evolutionarily neutral DNA base sequences were studied for 28 samples of Ligularia duciformis, L. kongkalingensis, and L. nelumbifolia collected in Sichuan and Yunnan Provinces of China. The samples could be classified into four chemotypes (1-4). Sesquiterpenoids having eremophilane and oplopane skeletons were isolated from two (Chemotype?1) and three (Chemotype?2) samples, respectively. Two new oplopane derivatives were isolated and their structures were determined. In 18 samples, phenylpropenoids were the major components (Chemotype?3). In five samples, neither phenylpropenoids nor sesquiterpenoids were found (Chemotype?4). Despite this large chemical variety, no correlation was found between the chemotype and the morphological criteria of species identification. The analysis of the evolutionarily neutral DNA regions also indicated that the samples were not separated into distinct clades and that introgression was extensive. 相似文献
87.
Abstract. 1. Damage to juvenile plant tissues can cause reductions in fitness. Therefore, plants are expected to have evolved various defences for juvenile organs; however, so far, little attention has been paid to mechanical defence, as they have been considered to constrain the growth of juvenile organs. This study revealed that the dioecious tree Aucuba japonica uses mechanical defences to protect young developing fruit from the gall midge Asphondylia aucubae. 2. Young fruit of A. japonica have a hard layer of endocarp covering the integument. Midges oviposit on the surface of the integument, where larval chambers are later formed. The endocarp gradually becomes cracked as the embryo sac develops. 3. Oviposition by the midges is successful only when the ovipositors happen to pass through cracks in the endocarp. Thus, to successfully lay eggs, midges must insert the ovipositor repeatedly. This should decrease the fecundity of the midges, and subsequently their infectiousness, because their adult lifespan is short and they do not consume food during this time. 4. Expansion of the cracks in the endocarp simplifies oviposition over time; however, the embryo sac continues to grow, increasing its volume relative to that of the ovule. This appears to deplete available space and tissue used in the construction of larval chambers, gradually making the fruit less susceptible to midge attacks. 5. The temporary nature of this defence should prevent it from constricting the growth of young fruit. This exemplifies a novel strategy for a mechanical defence of young developing plant tissues. 相似文献
88.
89.
Density-dependent mortality has been considered a symptom of intraspecific competition. We examined the occurrence of such
mortality in the early stages of gall induction by the gall midge Asphondylia aucubae Yukawa et Ohsaki (Diptera: Cecidomyiidae). Female midges deposit eggs into young fruit of the dioecious shrub Aucuba japonica Thunberg to induce gall formation. Each host fruit received 0–67 eggs (mean 18.5 eggs) from multiple females, whereas established
galls each contained one to ten larvae. Midges suffered intense mortality (65–90%) at the egg stage. Egg mortality occurred
even in fruit in which no larvae had hatched, suggesting that this mortality cannot be wholly attributed to larval interference.
Egg mortality was affected by fruit size, i.e., resource capacity. Midges distributed more eggs in larger fruit. Egg mortality
increased as the per-fruit density increased relative to fruit size. In contrast, the mortality of hatchlings was density-independent.
Our results suggest that A. aucubae intensely compete for gall-induction substrates, which are spatiotemporally rare resources. 相似文献
90.
The population dynamics of the aucuba fruit midge,Asphondylia aucubae (Japanese name: Aokimitamabae), were studied for 3 yr mainly at a broad-leaved evergreen forest on Mt. Shiroyama in Kagoshima
City. This species is univoltine and adults emerge in May. Eggs were laid randomly inside the immature fruit of the host plant,Aucuba japonica (Japanese name: Aoki). Healthy fruit fell by the following March, whereas galled fruit remained on the trees even after the
emergence of adult midges. The survival rate from the egg to adult stage was estimated to be 24.2%, and the number of midges
on five census trees increased gradually during the census period. Two important mortality factors were recognized, i.e.,
fall of incompletely galled fruit and damage of the galled fruit by feeding of caterpillars of a moth. Some unknown factors
were also found to be important, operating in an inversely density-dependent manner. The gradual increase ofA. aucubae is considered to be caused by such inversely density-dependent mortality processes. 相似文献