全文获取类型
收费全文 | 1044篇 |
免费 | 207篇 |
专业分类
1251篇 |
出版年
2021年 | 12篇 |
2019年 | 16篇 |
2018年 | 14篇 |
2017年 | 16篇 |
2016年 | 18篇 |
2015年 | 24篇 |
2014年 | 32篇 |
2013年 | 43篇 |
2012年 | 51篇 |
2011年 | 32篇 |
2010年 | 40篇 |
2009年 | 28篇 |
2008年 | 33篇 |
2007年 | 33篇 |
2006年 | 30篇 |
2005年 | 29篇 |
2004年 | 34篇 |
2003年 | 26篇 |
2002年 | 30篇 |
2001年 | 30篇 |
2000年 | 37篇 |
1999年 | 33篇 |
1998年 | 21篇 |
1997年 | 11篇 |
1996年 | 14篇 |
1995年 | 11篇 |
1994年 | 13篇 |
1993年 | 12篇 |
1992年 | 25篇 |
1991年 | 30篇 |
1990年 | 30篇 |
1989年 | 28篇 |
1988年 | 27篇 |
1987年 | 28篇 |
1986年 | 18篇 |
1985年 | 22篇 |
1984年 | 25篇 |
1983年 | 26篇 |
1982年 | 13篇 |
1981年 | 18篇 |
1980年 | 10篇 |
1979年 | 18篇 |
1978年 | 16篇 |
1977年 | 18篇 |
1976年 | 13篇 |
1975年 | 16篇 |
1974年 | 18篇 |
1973年 | 10篇 |
1970年 | 15篇 |
1969年 | 12篇 |
排序方式: 共有1251条查询结果,搜索用时 8 毫秒
11.
12.
Zusammenfassung Bei einem Patienten mit multiplen Mißbildungen wurde eine Duplikation für die distale Hälfte vom kurzen Arm des Chromosoms 2 und eine Defizienz an einem C-Chromosom gefunden. In der Literatur sind vier Fälle mit ähnlicher Duplikation, jedoch jeweils einer klein n Defizienz am Chromosom 3 beschrieben worden. Ein Vergleich der klinischen Merkmale bei den fünf Patienten zeigt weitgehende Übereinstimmungen. Es wird gefolgert, daß die gleichartige Duplikation für das einheitliche klinische Bild der Patienten verantwortlich ist. Es wurden Chromosomenmessungen, Analysen der Replikationsmuster und Meioseuntersuchungen durchgeführt. Die Genloci für das Ss- und das Rh-System konnten von einer Lokalisierung auf dem duplizierten Segment ausgeschlossen werden.
Mit Unterstützung durch die Deutsche Forschungsgemeinschaft. 相似文献
2/C translocation in father and daughter: 46,XY t (2p-;Cp+) and 46,XX Cp+
Summary In a patient with multiple anomalies, a duplication comprising the distal half of the short arm of chromosome 2 and a small deficiency of a C-chromosome was found. Four other cases from the literature exhibit a similar duplication combined with a small deficiency each of chromosome 3. Comparison of the clinical pictures of the five patients revealed a conformity in the major features. It is concluded that the duplication is responsible for the uniform appearance of these patients. The studies performed include chromosome measurements, examination of replication patterns and meiosis. The gene loci for the Ss and Rh systems could be excluded from localization on the duplicated segment.
Mit Unterstützung durch die Deutsche Forschungsgemeinschaft. 相似文献
13.
Summary Chromosome aberrations permit the assignment to and the exclusion of genes on certain chromosomes or definite segments. Only exclusion methods are discussed. All relevant data are compiled in a table from which it can be determined, which genetic systems are excluded from certain autosomal segments.
Direktor: Prof. Dr. Dr. H. Baitsch
Supported by the Deutsche Forschungsgemeinschaft. 相似文献
Zusammenfassung Angeborene Chromosomenaberrationen ermöglichen die Zuordnung und den Ausschluß von Genen auf dem betroffenen Segment. Nur die für Lokalisierungsausschlüsse verwendbaren Methoden werden diskutiert. Aus einer Zusammenstellung der bisherigen Befunde wird ermittelt, welche genetischen Systeme von einer Lokalisierung auf bestimmten Autosomen(segmenten) des Menschen ausgeschlossen werden können.
Direktor: Prof. Dr. Dr. H. Baitsch
Supported by the Deutsche Forschungsgemeinschaft. 相似文献
14.
Comparison of Germline Mosaics of Genes in the Polycomb Group of Drosophila Melanogaster 总被引:7,自引:0,他引:7 下载免费PDF全文
The genes of the Polycomb group (PcG) repress the genes of the bithorax and Antennapedia complexes, among others. To observe a null phenotype for a PcG gene, one must remove its maternal as well as zygotic contribution to the embryo. Five members of the PcG group are compared here: Enhancer of Polycomb [E(Pc)], Additional sex combs (Asx), Posterior sex combs (Psc), Suppressor of zeste 2 [Su(z)2] and Polycomblike (Pcl). The yeast recombinase (FLP) system was used to induce mitotic recombination in the maternal germline. Mutant embryos were analyzed by staining with antibodies against six target genes of the PcG. The loss of the maternal component leads to enhanced homeotic phenotypes and to unique patterns of misexpression. E(Pc) and Su(z)2 mutations had only subtle effects on the target genes, even when the maternal contributions were removed. Asx and Pcl mutants show derepression of the targets only in specific cell types. Psc shows unusual effects on two of the targets, Ultrabithorax and abdominal-A. These results show that the PcG genes do not act only in a common complex or pathway; they must have some independent functions. 相似文献
15.
R. Bender R. Fundele M. A. Surani L-L. Li R. Kothary D. O. Fürst B. Christ 《Development genes and evolution》1995,204(7-8):436-443
Parthenogenetic cells are lost from fetal chimeras. This may be due to decreased proliferative potential. To address this question, we have made use of combined cell lineage and cell proliferation analysis. Thus, the incorporation of bromodeoxyuridine in S-phase was determined for both parthenogenetic and normal cells in several tissues of fetal day 13 and 17 chimeras. A pronounced reduction of bromodesoxyuridine incorporation by parthenogenetic cells at both developmental stages was only observed in cartilage. In brain, skeletal muscle, heart and intestinal epithelium, this reduction was either less pronounced or observed only at one of the developmental stages analysed. No difference between parthenogenetic and normal cells was observed in epidermis and ganglia. Our results show that a loss of proliferative potential of parthenogenetic cells during fetal development contributes to their rapid elimination in some tissues. The analysis of the fate of parthenogenetic cells in skeletal muscle and cartilage development demonstrated different selection mechanisms in these tissues. In skeletal muscle, parthenogenetic cells were largely excluded from the myogenic lineage proper by early post-midgestation. In primary hyaline cartilage, parthenogenetic cells persisted into adulthood but were lost from cartilages that undergo ossification during late fetal development. 相似文献
16.
G. Mauff K. Bender Carolyn M. Giles S. Goldmann W. Opferkuch Barbara Wachauf 《Human genetics》1984,65(4):362-372
Summary Ten families with 82 members were investigated for C4A- and B polymorphism in a blind trial. Phenotyping was done on neuraminidase treated sera by immunofixation and simulataneously by hemolytic overlay electrophoresis. In addition Rg, Ch, BF, C2, HLA-A, B, C, DR, and GLO were determined. After decoding the samples the reliability of blind typing was found to be 84.4% according to segregation patters. Inconsistencies occurred mostly when A 4, A 2, or A 92 were present. The detection of silent A*Q0 and B*Q0 alleles was more critical than that of difficult allotypes. The quantitation of the C4A/B ratio by densitometry of stained gels or by conventional immunochemical measurements of serum C4 level could not substantially improve the identification of A*Q0 or B*Q0. C4 dependent activity in radial diffusion hemolysis showed satisfactory correspondence with the number of expressed C4B alleles. At least three haplotypes with two C4A genes (duplicated A genes) were observed as ascertained from offspring analysis in accordance with the MHC segregation pattern. Individuals with the duplicated C4A gene (C4A*3. A*2. in the absence of any other expressed A allele or together with C4A*92) showed only partial inhibition of Rodgers antisera. Partial inhibition of Chido antisera was seen in individuals with C4B 2 (in the absence of other B allotypes). The findings support the hypothesis of at least two structural C4 loci. The also demonstrate the inconsistency of quantitative data in the recognition of silent alleles. 相似文献
17.
Summary Measurements have been reported of carbon isotope ratios of timothy grown at different temperatures and with varying nitrogen and potassium supplies. Both total plant tissue and extracted plant tissue have been analyzed. The 13C/12C ratios were found to vary both with temperature and with nutrient level; the highest values of 13C were found under the most optimum growth conditions. 相似文献
18.
Effect of anoxia on intracellular ATP, Na+i, Ca2+i, Mg2+i, and cytotoxicity in rat hepatocytes. 总被引:4,自引:0,他引:4
A Gasbarrini A B Borle H Farghali C Bender A Francavilla D Van Thiel 《The Journal of biological chemistry》1992,267(10):6654-6663
The effects of anoxia were studied in freshly isolated rat hepatocytes maintained in agarose gel threads and perfused with Krebs-Henseleit bicarbonate buffer (KHB). Cytosolic free calcium (Ca2+i) was measured with aequorin, intracellular sodium (Na+i) with SBFI, intracellular pH (pHi) with BCECF, lactic dehydrogenase (LDH) by the increase in NADH absorbance during lactate oxidation to pyruvate, ATP by 31P NMR spectroscopy in real time, and intracellular free Mg2+ (Mg2+i) from the chemical shift of beta-ATP relative to alpha-ATP in the NMR spectra. Anoxia was induced by perfusing the cells with KHB saturated with 95% N2, 5% CO2. After 1 h of anoxia, beta-ATP fell 66%, and 85% after 2 h, while the Pi/ATP ratio increased 10-fold from 2.75 to 28.3. Under control conditions, the resting cytosolic free calcium was 127 +/- 6 nM. Anoxia increased Ca2+i in two distinct phases: a first rise occurred within 15 min and reached a mean value of 389 +/- 35 nM (p less than 0.001). A second peak reached a maximum value of 1.45 +/- 0.12 microM (p less than 0.001) after 1 h. During the first hour of anoxia, Na+i increased from 15.9 +/- 2.4 mM to 32.2 +/- 1.2 mM (p less than 0.001), Mg2+i doubled from 0.51 +/- 0.05 to 1.12 +/- 0.01 mM (p less than 0.001), and pHi decreased from 7.41 +/- 0.03 to 7.06 +/- 0.1 (p less than 0.001). LDH release doubled during the first hour and increased 6-fold during the second hour of anoxia. Upon reoxygenation, ATP, Ca2+i, Mg2+i, Na+i, and LDH returned near the control levels within 45 min. To determine whether the increased LDH release was related to the rise in Ca2+i, and whether the increased Ca2+i was caused by Ca2+ influx, the cells were perfused with Ca(2+)-free KHB (+ 0.1 mM EGTA) during the anoxic period. After 2 h of anoxia in Ca(2+)-free medium, beta-ATP again fell 90%, but Ca2+i, after the first initial peak, fell below control levels, and LDH release increased only 2.7-fold. During reoxygenation, Ca2+i, ATP, Na+i, and LDH returned near the control levels within 45 min. These results suggest that the rise in Ca2+i induced by anoxia is caused by an influx of Ca2+ from the extracellular fluid, and that LDH release and cell injury may be related to the resulting rise in Ca2+i. 相似文献
19.
K. Bender 《Human genetics》1982,61(2):127-134
Summary The 266 haplotypes associated with 133 reported HLA-A:HLA-B crossovers in Whites were distributed in a manner representative for European populations. From this observation it follows that differential recombination rates (haplotype-controlled crossover reduction or enhancement mechanisms) do not operate in the human histocompatibility complex.Supported by the Deutsche Forschungsgemeinschaft 相似文献
20.
Do benzodiazepines bind at adenosine uptake sites in CNS? 总被引:6,自引:0,他引:6
Benzodiazepines inhibit adenosine uptake into rat cerebral cortical synaptosomes and their potency as inhibitors of adenosine uptake is closely correlated with therapeutic efficacy. Agents which possess “benzodiazepine like” activities such as CL218,872, zopiclone and fominoben and which displace benzodiazepine binding to brain cell membranes, are also inhibitors of adenosine uptake into brain synaptosomes. The IC50 values of all these compounds as inhibitors of adenosine uptake are in close agreement with the IC50 values obtained for the displacement of benzodiazepine binding to the brain receptors. Adenosine uptake inhibitors (dipyridamole, hexobendine, papaverine, 6-(2-hydroxy-5-nitrobenzyl)thioguanosine) which competitively inhibit adenosine uptake, presumably by blocking adenosine binding to its carrier-protein, are competitive inhibitors of diazepam binding to the brain membrane receptors. The finding of a pronounced correlation between inhibition of benzodiazepine binding and inhibition of adenosine uptake further supports the proposal that benzodiazepines may exert part of their pharmacological action through the inhibition of adenosine uptake. 相似文献