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31.
32.
P Mancal R Benda J K?nig R Helm V N?mecek M Kyselová 《Journal of hygiene, epidemiology, microbiology, and immunology》1983,27(4):425-435
Two variants of sandwich-type ELISA (Enzyme Linked Immunosorbent Assay) kits for HBsAg detection (Sevatest ELISA HBsAg Macro I and Sevatest ELISA HBsAg Micro I) in human sera and plasmas were developed. As the solid phase, the ELISA Macro kit and ELISA Micro kit make use of polystyrene microtubes, and polystyrene microtitration plates, respectively, of Czechoslovak production (Koh-i-noor, Dalecín). Capture anti HBs antibody for adsorption to solid phase and rabbit anti HBs antibody for labelling with horse-radish peroxidase were prepared for both tests. The sensitivity of both ELISA kits for HBsAg, equal to approx. 2 ng/ml, was determined by titrating six selected HBsAg-positive sera and the WHO Agk 76 panel of HBsAg-positive sera and the results were compared with those obtained by ELISA, RIA (Radioimmunoassay) and RPHA (Reverse passive hemagglutination) kits of different producers and by counter-immunoelectrophoresis (CIEP). The sensitivity of the new ELISA kits was comparable to that of other producers' ELISA kits, higher than that of RPHA kits and only a little lower than that of RIA kits. A set of sera of patients hospitalised with different diagnoses was tested for HBsAg. The detection rate by ELISA Macro kit 2.8 and 1.5 times higher than by CIEP and RPHA (Raphadex B), respectively, and 1.1 time lower than by RIA (Austria II). 相似文献
33.
Résumé— Dans le but d'étudier la localisation cytologique de I'AChE dans divers tissus de Gymnote, nous avons préparé un antisérum anti-AChE en utilisant une préparation commerciale partiellement purifiée, contenant une forme globulaire de l'AChE G p . Cet antisérum réagit dans le test de double diffusion avec l'enzyme purifié G p , mais également avec la forme globulaire spontanée G b , ainsi qu'avec les trois espèces 'natives' asymétriques A , C et D . Nous avons mis en évidence l'existence de ces quatres formes G b , A , C et D dans des extraits de cerveau, de moelle épinière et de muscle de Gymnote. La forme globulaire G b est particulièrement abondante dans la moelle épinière et à un moindre degré, dans le cerveau. La distribution des espèes est identique dans le muscle et l'organe électrique. Grâce à notre antisérum, nous avom Iocalisé par immunofluorescence indirecte l'AChE dans les terminaisons nerveuses sur le muscle, dans le cytoplasme de certains neurones situés dans le centre moteur de la décharge (moelle épinière) et au voisinage du noyau central de la décharge (cerveau). 相似文献
34.
R. S. Benda 《Journal of fish biology》1981,18(5):609-613
A comparison of mean trawl catch rates (kg/h) from 128 collections in 1969 and 1970 and 63 collections in the fourth quarter of 1976 showed the following changes.
The trend that emerges shows a marked reduction in mean total trawl catch rates in the Kenya waters of Lake Victoria which should sound a note of caution for proponents of a mechanized fishing industry. 相似文献
- (1)
A reduction in mean total catch rates at all seven depth intervals from 1970–1976.
- (2)
Reductions of 6.8, 6.1, 3.9, 6.2, 1.5, 2.9 and 5.9 times from shallow to deepest 10m depths respectively.
- (3)
No Tilapiu esculenfu were collected in 1976.
The trend that emerges shows a marked reduction in mean total trawl catch rates in the Kenya waters of Lake Victoria which should sound a note of caution for proponents of a mechanized fishing industry. 相似文献
35.
Röhmel J Gerlinger C Benda N Läuter J 《Biometrical journal. Biometrische Zeitschrift》2006,48(6):916-933
In a clinical trial with an active treatment and a placebo the situation may occur that two (or even more) primary endpoints may be necessary to describe the active treatment's benefit. The focus of our interest is a more specific situation with two primary endpoints in which superiority in one of them would suffice given that non-inferiority is observed in the other. Several proposals exist in the literature for dealing with this or similar problems, but prove insufficient or inadequate at a closer look (e.g. Bloch et al. (2001, 2006) or Tamhane and Logan (2002, 2004)). For example, we were unable to find a good reason why a bootstrap p-value for superiority should depend on the initially selected non-inferiority margins or on the initially selected type I error alpha. We propose a hierarchical three step procedure, where non-inferiority in both variables must be proven in the first step, superiority has to be shown by a bivariate test (e.g. Holm (1979), O'Brien (1984), Hochberg (1988), a bootstrap (Wang (1998)), or L?uter (1996)) in the second step, and then superiority in at least one variable has to be verified in the third step by a corresponding univariate test. All statistical tests are performed at the same one-sided significance level alpha. From the above mentioned bivariate superiority tests we preferred L?uter's SS test and the Holm procedure for the reason that these have been proven to control the type I error strictly, irrespective of the correlation structure among the primary variables and the sample size applied. A simulation study reveals that the performance regarding power of the bivariate test depends to a considerable degree on the correlation and on the magnitude of the expected effects of the two primary endpoints. Therefore, the recommendation of which test to choose depends on knowledge of the possible correlation between the two primary endpoints. In general, L?uter's SS procedure in step 2 shows the best overall properties, whereas Holm's procedure shows an advantage if both a positive correlation between the two variables and a considerable difference between their standardized effect sizes can be expected. 相似文献
36.
An analysis of long-term changes in abundance of hibernating bats as revealed from the annual monitoring programme conducted
in four mountain regions of the Western Carpathians (Muránska planina Mts, Revúcka vrchovina Mts, Slovensky kras Mts, Štiavnické
vrchy Mts) during the period 1992–2009 is providing in the paper. Data from 52 hibernacula were analysed. Among 18 bat species
recorded, an apparent population increase of three most abundant thermophilous and originally cave dwelling species of bats,
Rhinolophus hipposideros, R. ferrumequinum, Myotis myotis, was observed. In other bat species (e.g., R. euryale, M. emarginatus, M. mystacinus, M. dasycneme, Barbastella barbastellus), population trends could not be detected and because of data scarcity, they should be evaluated from more extensive datasets
obtained from a wide range of hibernacula or from a completely different type of evidence. 相似文献
37.
F Rieger S Bon J Massoulié J Cartauld B Picard P Benda 《European journal of biochemistry》1976,68(2):513-521
Electron microscopy, sequential degradation by hydrolytic enzymes and the physical-chemical properties of the molecular forms of Torpedo acetylcholinesterase indicate that these molecules are structurally related to each other in the same way as the molecular forms of Electrophorus acetylcholinesterase: all are derived from a complex structure in which three tetrameric groups of subunits are associated with a rod-like 'tail'. In aged preparations the catalytic subunits are split into fragments in a manner similar to those of Electrophorus acetylcholinesterase. Immunological cross-reaction between both enzymes demonstrates the occurrence of common antigenic sites. The enzymes from the two sources, however, are different in their molecular weights and susceptibility to hydrolytic enzymes. Also, Torpedo acetylcholinesterase does not precipitate with either isologous or heterologous antibodies. 相似文献
38.
Benda R Tse Sum Bui B Schünemann V Florentin D Marquet A Trautwein AX 《Biochemistry》2002,41(50):15000-15006
Biotin synthase, the enzyme that catalyzes the last step of the biosynthesis of biotin, contains only [2Fe-2S](2+) clusters when isolated under aerobic conditions. Previous results showed that reconstitution with an excess of FeCl(3) and Na(2)S under reducing and anaerobic conditions leads to either [4Fe-4S](2+), [4Fe-4S](+), or a mixture of [4Fe-4S](2+) and [2Fe-2S](2+) clusters. To determine whether any of these possibilities or other different cluster configuration could correspond to the physiological in vivo state, we have used (57)Fe M?ssbauer spectroscopy to investigate the clusters of biotin synthase in whole cells. The results show that, in aerobically grown cells, biotin synthase contains a mixture of [4Fe-4S](2+) and [2Fe-2S](2+) clusters. A mixed [4Fe-4S](2+):[2Fe-2S](2+) cluster form has already been observed under certain in vitro conditions, and it has been proposed that both clusters might each play a significant role in the mechanism of biotin synthase. Their presence in vivo is now another argument in favor of this mixed cluster form. 相似文献
39.
40.
Dennis Kappei Falk Butter Christian Benda Marion Scheibe Irena Draškovič Michelle Stevense Clara Lopes Novo Claire Basquin Masatake Araki Kimi Araki Dragomir Blazhev Krastev Ralf Kittler Rolf Jessberger J Arturo Londoño‐Vallejo Matthias Mann Frank Buchholz 《The EMBO journal》2013,32(12):1681-1701
Telomeres are repetitive DNA structures that, together with the shelterin and the CST complex, protect the ends of chromosomes. Telomere shortening is mitigated in stem and cancer cells through the de novo addition of telomeric repeats by telomerase. Telomere elongation requires the delivery of the telomerase complex to telomeres through a not yet fully understood mechanism. Factors promoting telomerase–telomere interaction are expected to directly bind telomeres and physically interact with the telomerase complex. In search for such a factor we carried out a SILAC‐based DNA–protein interaction screen and identified HMBOX1, hereafter referred to as homeobox telomere‐binding protein 1 (HOT1). HOT1 directly and specifically binds double‐stranded telomere repeats, with the in vivo association correlating with binding to actively processed telomeres. Depletion and overexpression experiments classify HOT1 as a positive regulator of telomere length. Furthermore, immunoprecipitation and cell fractionation analyses show that HOT1 associates with the active telomerase complex and promotes chromatin association of telomerase. Collectively, these findings suggest that HOT1 supports telomerase‐dependent telomere elongation. 相似文献