全文获取类型
收费全文 | 179篇 |
免费 | 21篇 |
出版年
2021年 | 6篇 |
2020年 | 6篇 |
2019年 | 10篇 |
2018年 | 6篇 |
2017年 | 5篇 |
2016年 | 14篇 |
2015年 | 12篇 |
2014年 | 6篇 |
2013年 | 4篇 |
2012年 | 9篇 |
2011年 | 6篇 |
2010年 | 6篇 |
2009年 | 3篇 |
2008年 | 2篇 |
2007年 | 4篇 |
2006年 | 8篇 |
2005年 | 6篇 |
2004年 | 3篇 |
2003年 | 3篇 |
2002年 | 5篇 |
2000年 | 5篇 |
1999年 | 2篇 |
1998年 | 7篇 |
1996年 | 1篇 |
1995年 | 3篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 4篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 7篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 2篇 |
1978年 | 3篇 |
1977年 | 5篇 |
1976年 | 3篇 |
1975年 | 4篇 |
1974年 | 1篇 |
1973年 | 5篇 |
1972年 | 1篇 |
1971年 | 1篇 |
1970年 | 1篇 |
1969年 | 1篇 |
1968年 | 2篇 |
1965年 | 1篇 |
1961年 | 2篇 |
排序方式: 共有200条查询结果,搜索用时 15 毫秒
21.
22.
Facchini FD Vici AC Benassi VM Freitas LA Reis RA Jorge JA Terenzi HF Polizeli Mde L 《Bioprocess and biosystems engineering》2011,34(8):1027-1038
Fibrolytic enzyme production by Aspergillus japonicus C03 was optimized in a medium containing agro-industrial wastes, supplemented with peptone and yeast extract. A 23 full factorial composite and response surface methodology were used to design the experiments and analysis of results. Tropical
forages were hydrolyzed by A. japonicus C03 enzymatic extract in different levels, and they were also tested as enzymatic substrate. Optimal production to xylanase
was obtained with soybean bran added to crushed corncob (1:3), 0.01% peptone, and 0.2% yeast extract, initial pH 5.0, at 30 °C
under static conditions for 5 days of incubation. Optimal endoglucanase production was obtained with wheat bran added to sugarcane
bagasse (3:1), 0.01% peptone, and 0.2% yeast extract, initial pH 4.0, at 30 °C, for 6 days, under static conditions. Addition
of nitrogen sources as ammonium salts either inhibited or did not influence xylanase production. This enzymatic extract had
a good result on tropical forage hydrolyzes and showed better performance in the Brachiaria genera, due to their low cell wall lignin quantity. These results represent a step forward toward the use of low-cost agricultural
residues for the production of valuable enzymes with potential application in animal feed, using fermentation conditions. 相似文献
23.
Michele Michelin Vivian M. Benassi Luiz Alberto B. Moraes João A. Jorge Maria de Lourdes T.M. Polizeli 《Carbohydrate research》2010,345(16):2348-2353
An α-amylase produced by Paecilomyces variotii was purified by DEAE-cellulose ion exchange chromatography, followed by Sephadex G-100 gel filtration and electroelution. The α-amylase showed a molecular mass of 75 kDa (SDS-PAGE) and pI value of 4.5. Temperature and pH optima were 60 °C and 4.0, respectively. The enzyme was stable for 1 h at 55 °C, showing a t50 of 53 min at 60 °C. Starch protected the enzyme against thermal inactivation. The α-amylase was more stable in alkaline pH. It was activated mainly by calcium and cobalt, and it presented as a glycoprotein with 23% carbohydrate content. The enzyme preferentially hydrolyzed starch and, to a lower extent, amylose and amylopectin. The Km of α-amylase on Reagen® and Sigma® starches were 4.3 and 6.2 mg/mL, respectively. The products of starch hydrolysis analyzed by TLC were oligosaccharides such as maltose and maltotriose. The partial amino acid sequence of the enzyme presented similarity to α-amylases from Bacillus sp. These results confirmed that the studied enzyme was an α-amylase ((1→4)-α-glucan glucanohydrolase). 相似文献
24.
Bioinsecticides are important in the control of disease vectors, but data regarding their physiological effects on target insects are incomplete. This study describes morphological changes that occur in the midgut of third instar Aedes aegypti L. (Diptera: Culicidae) following treatment with a methanolic extract of Annona coriacea (Magnoliales: Annonaceae). Dissected midguts were subdivided into anterior and posterior regions and analyzed by light and scanning electron microscopy. Insects exposed to the extract displayed intense, destructive cytoplasmic vacuolization in columnar and regenerative midgut cells. The apical surfaces of columnar cells exhibited cytoplasmic protrusions oriented toward the lumen, suggesting that these cells could be involved in apocrine secretory processes and/or apoptosis. We report that A. coriacea extracts induced morphological alterations in the midgut of A. aegypti midgut larvae, supporting the use of plant extracts for control of the dengue vector. 相似文献
25.
Piernicola Pedicini Rocchina Caivano Barbara A Jereczek-Fossa Lidia Strigari Barbara Vischioni Daniela Alterio Marta Cremonesi Francesca Botta Antonio Nappi Giuseppina Improta Giovanni Storto Alba Fiorentino Marcello Benassi Roberto Orecchia Vincenzo Fusco 《Theoretical biology & medical modelling》2012,9(1):1-1
26.
G Benassi 《Experimental cell research》1968,50(1):159-166
27.
28.
The partitioning of limited resources commonly explains how different species can coexist within the same ecological community. In this 2010 study, the diets of three coexisting freshwater fishes (Cape galaxias Galaxias zebratus, n = 27; Cape kurper Sandelia capensis, n = 60; Breede River redfin Pseudobarbus burchelli, n = 77) were characterised and compared in three headwater streams in South Africa's Cape Fold Ecoregion using gut contents and stable isotope analyses. These data were analysed to ascertain whether the three species exploit distinct trophic niches. Both approaches provided evidence that these species occupy different trophic niches, though with some overlap. However, dietary differences among sites were not consistent and were probably influenced by site-specific factors like resource availability. Pseudobarbus burchelli had a broader niche breadth at Tierkloof Stream than the other two species, but not at Waaihoek or Tierstel Streams. Our results also suggest that P. burchelli consumed a more omnivorous diet than do the other two species, whereas S. capensis occupied a higher trophic position than the other two species and consumed vertebrates. Our findings suggest that these species occupy non-equivalent feeding niches in Cape Fold Ecoregion headwater streams, and that diet partitioning might facilitate their coexistence in these systems. 相似文献
29.
30.