全文获取类型
收费全文 | 6843篇 |
免费 | 620篇 |
国内免费 | 69篇 |
出版年
2023年 | 50篇 |
2022年 | 93篇 |
2021年 | 194篇 |
2020年 | 129篇 |
2019年 | 180篇 |
2018年 | 190篇 |
2017年 | 201篇 |
2016年 | 242篇 |
2015年 | 414篇 |
2014年 | 403篇 |
2013年 | 521篇 |
2012年 | 607篇 |
2011年 | 554篇 |
2010年 | 399篇 |
2009年 | 350篇 |
2008年 | 419篇 |
2007年 | 360篇 |
2006年 | 325篇 |
2005年 | 278篇 |
2004年 | 299篇 |
2003年 | 217篇 |
2002年 | 225篇 |
2001年 | 69篇 |
2000年 | 44篇 |
1999年 | 51篇 |
1998年 | 48篇 |
1997年 | 45篇 |
1996年 | 37篇 |
1995年 | 37篇 |
1994年 | 34篇 |
1993年 | 40篇 |
1992年 | 30篇 |
1991年 | 30篇 |
1990年 | 36篇 |
1989年 | 32篇 |
1988年 | 19篇 |
1987年 | 20篇 |
1986年 | 18篇 |
1985年 | 27篇 |
1984年 | 29篇 |
1983年 | 18篇 |
1982年 | 25篇 |
1981年 | 16篇 |
1980年 | 16篇 |
1979年 | 15篇 |
1978年 | 12篇 |
1977年 | 11篇 |
1976年 | 11篇 |
1975年 | 12篇 |
1967年 | 7篇 |
排序方式: 共有7532条查询结果,搜索用时 559 毫秒
241.
Justin O'Connor Andrew Pickworth Ben Fanson Denis Lovric 《Ecological Management & Restoration》2019,20(2):151-155
In the Barwon River, Australia, a tidal barrage formed a major impediment to fish movement so in 2013 a vertical slot fishway was installed. The assessment of fishways on tidal barriers is rare in Australia so to ensure the fishway was achieving its ecological objective (i.e. successfully passing the target size range of fish of 20–400 mm total length), fish were trapped at the entrance and exit on 12 occasions and the species composition, abundance and length of fish at the two locations were compared. Additionally, a section of the river downstream of the fishway was sampled to ensure fishway trapping accurately reflected the species composition wanting to use the fishway to move upstream. Eighteen species and 69,246 individual fish were caught in the fishway traps. Catch rates between locations did not differ for Common Galaxias (Galaxias maculatus) or Australian Smelt (Retropinna semoni), although species‐specific catch rates were lower at the exit for Flat‐headed Gudgeon (Philypnodon grandiceps), Tupong (Pseudaphritis urvillii) and Yellow‐eye Mullet (Aldrichetta forsteri). Length distribution between locations only differed for Australian Smelt with small fish under‐represented at the exit location (<25 mm total length). Eight species of fish were collected downstream of the fishway that were not collected in it; however, all of these were estuarine dependent except the non‐native Common Carp (Cyprinus carpio). Our results indicate that vertical slot fishways are a suitable design for improving river connectivity at a low head, tidal barrages in south‐eastern Australia. The study reiterates the importance of reinstating connectivity for species with obligate marine/freshwater migratory life history traits, and the indirect benefits of increased productivity made available to upstream areas. 相似文献
242.
Marine Schoeffler Anne-Laure Gaudin Fanny Ramel Odile Valette Yann Denis Wagdi Ben Hania Agnès Hirschler-Réa Alain Dolla 《Environmental microbiology》2019,21(1):360-373
Desulfovibrio species are representatives of microorganisms at the boundary between anaerobic and aerobic lifestyles, since they contain the enzymatic systems required for both sulfate and oxygen reduction. However, the latter has been shown to be solely a protective mechanism. By implementing the oxygen-driven experimental evolution of Desulfovibrio vulgaris Hildenborough, we have obtained strains that have evolved to grow with energy derived from oxidative phosphorylation linked to oxygen reduction. We show that a few mutations are sufficient for the emergence of this phenotype and reveal two routes of evolution primarily involving either inactivation or overexpression of the gene encoding heterodisulfide reductase. We propose that the oxygen respiration for energy conservation that sustains the growth of the O2-evolved strains is associated with a rearrangement of metabolite fluxes, especially NAD+/NADH, leading to an optimized O2 reduction. These evolved strains are the first sulfate-reducing bacteria that exhibit a demonstrated oxygen respiratory process that enables growth. 相似文献
243.
Ben Swallow Stephen T. Buckland Ruth King Mike P. Toms 《Ecology and evolution》2019,9(21):12182-12192
The factors governing the recent declines observed in many songbirds have received much research interest, in particular whether increases of avian predators have had a negative effect on any of their prey species. In addition, further discussion has centered on whether or not the choice of model formulation has an effect on model inference. The study goal was to evaluate changes in the number of 10 songbird species in relation to a suite of environmental covariates, testing for any evidence in support of a predator effect using multiple model formulations to check for consistency in the results. We compare two different approaches to the analysis of long‐term garden bird monitoring data. The first approach models change in the prey species between 1970 and 2005 as a function of environmental covariates, including the abundance of an avian predator, while the second uses a change–change approach. Significant negative relationships were found between Eurasian Sparrowhawk Accipiter nisus and three of the 10 species analyzed, namely house Sparrow Passer domesticus, starling Sturnus vulgaris, and blue tit Cyanistes caeruleus. The results were consistent under both modeling approaches. It is not clear if this is a direct negative impact on the overall populations of these species or a behavioral response of the prey species to avoid feeding stations frequented by Sparrowhawks (which may in turn have population consequences, by reducing available resources). The species showing evidence of negative effects of Sparrowhawks were three of the four species most at risk to Sparrowhawk predation according to their prevalence in the predator's diet. The associations could be causal in nature, although in practical terms the reduction in the rate of change in numbers visiting gardens accredited to Sparrowhawks is relatively small, and so unlikely to be the main driver of observed population declines. 相似文献
244.
Cindy Q. Tang Yongchuan Yang Arata Momohara Huan-Chong Wang Hong Truong Luu Shuaifeng Li Kun Song Shenhua Qian Ben LePage Yi-Fei Dong Peng-Bin Han Masahiko Ohsawa Buu Thach Le Huu Dang Tran Minh Tri Dang Ming-Chun Peng Chong-Yun Wang 《Plant Diversity》2019,(4):237-249
The Chinese water pine Glyptostrobus pensilis is the sole surviving species of the genus Glyptostrobus. It is endemic to southern China, central Vietnam, and eastern Laos, and today it is nearly extinct in the wild. Forest community characteristics and population structure of G. pensilis in China have remained un-known up to now. We investigated six swamp forest stands and analyzed their forest community characteristics (i.e. vertical stratification, species composition, and diversity) and population structure, including the frequency distribution of DBH (diameter at breast height) and age-classes as found in Fujian Province, southeastern China. The vertical stratifications of all the forest stands were rather simple. The remaining wild specimens ranged from roughly 15 to some 357 years for an average of ca. 85 years, with only a few individuals less than 20 years old. Compared with the stands and populations of G. pensilis in Vietnam, the taxonomic compositions of the stands in the two regions were different, except for the dominant species-G. pensilis. The Shannon-Wiener index showed the overstory of each stand had much lower diversity (0.26 on average) in Fujian Province than that (1.97 on average) in Vietnam, whereas the diversity indices were about the same (around 2.41) for the understories in the two regions. Furthermore, we discovered 18 G. pensilis seedlings at the study sites in Fujian Province. This discovery demonstrates that G. pensilis regeneration is extremely poor and its populations are declining, although these populations are rela-tively healthier than those in Vietnam. 相似文献
245.
Klibi N Ben Slama K Sáenz Y Masmoudi A Zanetti S Sechi LA Boudabous A Torres C 《Canadian journal of microbiology》2007,53(3):372-379
Phenotypic and genotypic determination of virulence factors were carried out in 46 high-level gentamicin-resistant (HLGR) clinical Enterococcus faecalis (n=34) and Enterococcus faecium (n=12) isolates recovered from different patients in La Rabta Hospital in Tunis, Tunisia, between 2000 and 2003 (all these isolates harboured the aac(6')-aph(2") gene). The genes encoding virulence factors (agg, gelE, ace, cylLLS, esp, cpd, and fsrB) were analysed by PCR and sequencing. The production of gelatinase and hemolysin, the adherence to caco-2 and hep-2 cells, and the capacity for biofilm formation were investigated in all 46 HLGR enterococci. The percentages of E. faecalis isolates harbouring virulence genes were as follows: gelE, cpd, and ace (100%); fsrB (62%); agg (56%); cylLLS (41.2%); and esp (26.5%). The only virulence gene detected among the 12 HLGR E. faecium isolates was esp (58%). Gelatinase activity was detected in 22 of the 34 E. faecalis isolates (65%, most of them with the gelE+-fsrB+ genotype); the remaining 12 isolates were gelatinase-negative (with the gelE+-fsrB- genotype and the deletion of a 23.9 kb fragment of the fsr locus). Overall, 64% of the cylLLS-containing E. faecalis isolates showed beta-hemolysis. A high proportion of our HLGR E. faecalis isolates, in contrast to E. faecium, showed moderate or strong biofilm formation or adherence to caco-2 and hep-2 cells. 相似文献
246.
247.
248.
The effects of environmental heterogeneity on multivariate selection on reproductive traits in female great tits 总被引:1,自引:0,他引:1
Garant D Kruuk LE McCleery RH Sheldon BC 《Evolution; international journal of organic evolution》2007,61(7):1546-1559
Describing natural selection on phenotypic traits under varying environmental conditions is essential for a quantitative assessment of the scale at which adaptation might occur and of the impact of environmental variability on evolution. Here we analyzed patterns of multivariate selection via fecundity and viability on three reproductive traits (laying date, clutch size, and egg weight) in a population of great tits (Parus major). We quantified selection under different environmental conditions using (1) local variation in breeding density and (2) distinct areas of the population's habitat. We found that selection gradients were generally stronger for fecundity than for viability selection. We also found correlational selection acting on the combination of laying date and clutch size; this is the first documented evidence of such selection acting on these two traits in a passerine bird. Our analyses showed that both local breeding density and habitat significantly influenced selection patterns, hence favoring different patterns of reproductive investment at a small-scale relative to typical dispersal distances in this species. Canonical rotation of the nonlinear selection matrices yielded similar conclusions as traditional nonlinear selection analyses, and also showed that the main axes of selection and fitness surfaces varied over space within the population. Our results emphasize the importance of quantifying different forms of selection, and of including variation in environmental conditions at small scales to gain a better understanding of potential evolutionary dynamics in wild populations. This study suggests that the fitness landscape for this species is relatively rugged at scales relevant to the life histories of individual birds and their close relatives. 相似文献
249.
250.
Functional dependencies between genes are a defining characteristic of gene networks underlying quantitative traits. However, recent studies show that the proportion of the genetic variation that can be attributed to statistical epistasis varies from almost zero to very high. It is thus of fundamental as well as instrumental importance to better understand whether different functional dependency patterns among polymorphic genes give rise to distinct statistical interaction patterns or not. Here we address this issue by combining a quantitative genetic model approach with genotype-phenotype models capable of translating allelic variation and regulatory principles into phenotypic variation at the level of gene expression. We show that gene regulatory networks with and without feedback motifs can exhibit a wide range of possible statistical genetic architectures with regard to both type of effect explaining phenotypic variance and number of apparent loci underlying the observed phenotypic effect. Although all motifs are capable of harboring significant interactions, positive feedback gives rise to higher amounts and more types of statistical epistasis. The results also suggest that the inclusion of statistical interaction terms in genetic models will increase the chance to detect additional QTL as well as functional dependencies between genetic loci over a broad range of regulatory regimes. This article illustrates how statistical genetic methods can fruitfully be combined with nonlinear systems dynamics to elucidate biological issues beyond reach of each methodology in isolation. 相似文献