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91.
M E Eremeeva E B Lapina N M Balaeva V F Ignatovich L S Belousova 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》1989,(5):20-26
PAAG-electrophoresis of the isogenic pair of Rickettsia prowazekii strains E and Evir lysates demonstrate the similarity in polypeptide tracks. The different electrophoretic mobility of the Mr 30 Kd protein from these strains as compared with the mobility of analogous protein from the standard virulent Breinl strain is registered. In immunoblot experiments the specific rabbit antiserums obtained on the 30th day of infection with the Breinl, E or Evir strains demonstrate the presence of the different main antigens 60 Kd or 70 Kd. The difference evidently reflects the specificity of development of two forms of infection by the strains having different virulence. The surface tris-soluble antigens of Rickettsia prowazekii have the similar polypeptide contents and immunochemical properties. The main component of tris-soluble antigens Mr 130 Kd protein is not strain specific having the common thermolabile epitope. 相似文献
92.
V V Belousova A M Dudchenko L D Luk'ianova 《Biulleten' eksperimental'no? biologii i meditsiny》1992,114(12):588-590
It is shown that rat liver cells have different sensitivity to oxygen deficiency. Hepatocytes of highly resistant animals have higher viability and maintain higher ATP levels during acute hypoxia. The data obtained enable us to suppose that liver cells of highly resistant rats are more resistant to oxygen deficiency than nonresistant ones. 相似文献
93.
Background
MicroRNAs (miRNAs) are oligoribonucleotides with an important role in regulation of gene expression at the level of translation. Despite imperfect target complementarity, they can also significantly reduce mRNA levels. The validity of miRNA target gene predictions is difficult to assess at the protein level. We sought, therefore, to determine whether a general lowering of predicted target gene mRNA expression by endogenous miRNAs was detectable within microarray gene expression profiles. 相似文献94.
Zubkova OV Logunov DIu Karpov AP Shmarov MM Belousova RV Naroditskiĭ BS 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》2008,(2):32-36
Recombinant avian adenovirus CELO bearing sequence RGD in the structure of a HI-loop of long fiber was designed. Experiments in vitro revealed that introduction of RGD-motif into fiber of CELO increased the ability of the virus to be attached to a surface of CAR-negative cells, and raised efficiency of the process of internalization of the virus both in CAR-positive, and in CAR-negative cells. 相似文献
95.
Khlimankov DIu Rechkunova NI Khodyreva SN Petruseva IO Nazarkina ZhK Belousova EA Lavrik OI 《Molekuliarnaia biologiia》2002,36(6):1044-1053
Nicks and flaps are intermediates in various processes of DNA metabolism, including replication and repair. Photoaffinity modification was employed in studying the interaction of the replication protein A (RPA) and flap endonuclease 1 (FEN-1) with DNA duplexes similar to structures arising during long-patch base excision repair. The proteins were also tested for effect on DNA polymerase beta (Pol beta) interaction with DNA. Using Pol beta, a photoreactive dTTP analog was added to the 3' end of an oligonucleotide flanking a nick or a flap in DNA intermediates. The character and intensity of protein labeling depended on the type of intermediates and on the presence of the phosphate or tetrahydrofuran at the 5' end of a nick or a flap. Photoaffinity labeling of Pol beta substantially (up to three times) increased in the presence of RPA or FEN-1. Various DNA substrates were used to study the effects of RPA and FEN-1 on Pol beta-mediated DNA synthesis with displacement of a downstream primer. In contrast to FEN-1, RPA had no effect on DNA repair synthesis by Pol beta during long-patch base excision repair. 相似文献
96.
We tested the effects of injections of small doses of131I (150 μCi) into female rats on brain development in their offspring. The brains of rats whose embryogenesis was influenced
by incorporated131I were studied with the use of an immunohistochemical technique (monoclonal antibodies to nuclear antigen of cell proliferation).
Single injections of131I in the above dose were shown to noticeably suppress the cell proliferative activity in a few structures of the brain of
newborn rats. The most significant drop in the proliferative index in the external layers of the cerebellum was observed after131I injections performed on the 16th day of embryogenesis (when the embryonal thyroid gland begins its functioning). 相似文献
97.
We studied the effects of ultrasound treatment on an increase of the yield of amino acid mixtures from autolysates of Saccharomyces cerevisiae VKM-Y-2465 grown on ethanol. Adding 2% chloroform (activating agent), autolysis for 5-10 h, and ultrasound treatment of autolysates allowed us to obtain mixtures balanced in lysine, methionine, and tryptophan and containing up to 90% free amino acids. 相似文献
98.
Kaplin V. G. Katyuk A. I. Vasin V. G. Belousova O. A. Vasin A. V. 《Entomological Review》2019,99(5):580-597
Entomological Review - This paper summarizes data on seasonal development and fecundity of the pea weevil Bruchus pisorum L., its impact on the chemical composition of the pea seeds, damage to... 相似文献
99.
D Iu Khlimankov I O Petruseva N I Rechkunova E A Belousova D M Kolpashchikov S N Khodyreva O I Lavrik 《Bioorganicheskaia khimiia》2001,27(3):205-209
To introduce photoreactive dNTP residues to the 3'-end of a mononucleotide gap, base-substituted photoreactive deoxynucleoside triphosphate derivatives, (5-[N-(2,3,5,6-tetrafluoro-4-azidobenzoyl)-trans-3-aminopropenyl-1]- and 5-(N-[N-(4-azido-2,5-difluoro-3-chloropyridine-6-yl)-3-aminopropionyl]- trans-3-aminopropenyl-1)-2'-deoxyuridine 5'-triphosphates, were used as substrates in the DNA polymerase beta-catalyzed reaction. The resulting nick, containing a modified base at the 3'-end, was sealed by T4 phage DNA ligase. This approach enables the preparation of DNA duplexes bearing photoreactive groups at predetermined position(s) of the nucleotide chain. Using the generated photoreactive DNA duplexes, the photoaffinity modifications of DNA polymerase beta and human replicative protein A (hRPA) were carried out. It was shown that DNA polymerase beta and hRPA subunits were modified with the photoreactive double-stranded DNA considerably less effectively than by the nicked DNA. In the case of double-stranded DNA, the hRPA p70 subunit was preferentially labeled, implying a crucial role of this subunit in the protein-DNA interaction. 相似文献
100.
T Williams E Walz AR Lane M Pebole AC Hackney 《Biology of sport / Institute of Sport》2015,32(3):193-198
This study assessed the influence of estrogen (E2) on muscle damage biomarkers [skeletal muscle - creatine kinase (CK); cardiac muscle - CK-MB] responses to prolonged aerobic exercise. Eumenorrheic women (n=10) who were physically active completed two 60-minute treadmill running sessions at ∼60-65% maximal intensity during low E2 (midfollicular menstrual phase) and high E2 (midluteal menstrual phase) hormonal conditions. Blood samples were collected prior to exercise (following supine rest), immediately post-, 30 min post-, and 24 hours post-exercise to determine changes in muscle biomarkers. Resting blood samples confirmed appropriate E2 hormonal levels Total CK concentrations increased following exercise and at 24 hours post-exercise were higher in the midfollicular low E2 phase (p<0.001). However, CK-MB concentrations were unaffected by E2 level or exercise (p=0.442) resulting in the ratio of CK-MB to total CK being consistently low in subject responses (i.e., indicative of skeletal muscle damage). Elevated E2 levels reduce the CK responses of skeletal muscle, but had no effect on CK-MB responses following prolonged aerobic exercise. These findings support earlier work showing elevated E2 is protective of skeletal muscle from exercise-induced damage associated with prolonged aerobic exercise. 相似文献