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51.
Prof. Dr. Belma Konuklugil Dr. Ibrahim Seyda Uras Dr. Baris Karsli Dr. Ayse Demirbas 《化学与生物多样性》2023,20(9):e202300744
This study, it was aimed to examine the change in the antimicrobial effect of sea anemone Parazoanthus axinellae extract by forming its nanoflowers. A scanning electron microscope (SEM) and energy dispersive X-ray spectroscopy (EDX) were expended to observe the morphologies of the Cu NFs that had been produced. Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) techniques were expended to analyze the managing assemblies in P. axinellae extract, which perform an effective part in the synthesis routine, as well as the crystal assembly of NFs. P. axinellae extract mediated the HNFs (Hybrid nanoflowers) are at high, pure crystalline nature, flower shape with a crystallographic system at the nanoscale with mean crystallite size 21.9 nm using XRD, and average particle size ~10 nm by SEM. The broad absorption band at 2981–2915 cm−1 in the FT-IR spectra of anemone extract and Cu-anemone NFs represents the unique peak of hydroxy groups. In addition, Cu NFs were tested for their antibacterial properties. Cu NFs have been discovered to exhibit antibacterial properties. It is suggested that P. axinellae extract and various inorganic components be used to synthesize a variety of NFs and assess their suitability for usage in biomedical fields. 相似文献
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Belma Turan Ertan Delilba§i Nejat Dalay §evki Sert Lale Afrasyap Ahmet Sayal 《Biological trace element research》1992,33(1-3):95-102
Selenium, aluminum, cadmium, and magnesium concentrations and gluthathione-peroxidase activities in sera of 35 healthy individuals,
30 renal transplants, and 30 hemodialysis patients were measured. Serum selenium, aluminum, and cadmium concentrations in
both groups of patients were higher than the controls (p<0.001), whereas the serum gluthathione-peroxidase levels were lower (p<0.001). According to our results, it can be concluded that the patients receiving hemodialysis are subjected to more toxic
elements than the transplantation patients. These findings imply that dietary selenium supplement may be suggested in renal
failure for the detoxification of elements, such as cadmium and mercury. The essential trace element selenium takes part not
only in the direct protection of endothelial cells against the accumulation of aggressive oxygen species, but also in the
preventions of the toxic effects of cadmium or in the modulation of the active calcium transport. 相似文献
53.
The changes in distribution of several important mineral nutrients (N, K, Ca, Mg, Mn, and Fe) were studied in relation to monocarpic senescence (measured as leaf yellowing) and fruit development in hydroponically-grown (and to a lesser extent field-grown) Anoka soybeans with particular emphasis on the leaves and seeds. Only N shows a clear redistribution from the leaves to the seeds as the seeds grow, and this transfer starts before visible leaf yellowing. K, Ca, Mn and Fe do not seem to redistribute, but Mg may undergo limited redistribution. Depodding prevents the drop in the amounts of minerals in attached leaves by blocking leaf shedding and/or redistribution and also creates some quantitative changes in mineral distribution. On a g fresh weight basis, only the N content of leaf blades decreases during yellowing; the K, Mg, Ca, Mn and Fe contents do not decrease. Therefore, depletion of the latter minerals from the leaves cannot be responsible for their yellowing. Although N deficiency alone could cause foliar chlorosis, the monocarpic yellowing pattern is distinctly different from that induced by N deprivation. 相似文献
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It is known that selenium compounds can restore some metabolic parameters in experimental diabetes. However, as there are
no, clear data about their effects on the altered antioxidant defense system of the diabetic heart, we aimed to investigate
whether these beneficial effects extend to the alterations of some enzyme activities, which play important roles in antioxidant
defense system. Diabetes was induced by streptozotocin (50 mg/kg body weight) and rats were then treated with sodium selenite
(5 μmol/kg/d) for 4 wk. Sodium selenite treatment of the diabetic rats significantly restored the altered activities of glutathione-
S-transferase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase, which are involved in the glutathione
metabolism of the heart, but slightly but significantly decreased the high blood glucose level. In summary, the present study
suggests that the beneficial effects of sodium selenite treatment appears to be the result of the restoration altered activities
of the antioxidant enzymes in diabetic heart tissue. 相似文献
55.
Increased free Zn2+ correlates induction of sarco(endo)plasmic reticulum stress via altered expression levels of Zn2+‐transporters in heart failure
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Erkan Tuncay Ceylan Verda Bitirim Evren Ozcinar Mustafa Bahadir Inan Zeynep Tokcaer‐Keskin Kamil Can Akcali Ahmet Ruchan Akar Belma Turan 《Journal of cellular and molecular medicine》2018,22(3):1944-1956
Zn2+‐homoeostasis including free Zn2+ ([Zn2+]i) is regulated through Zn2+‐transporters and their comprehensive understanding may be important due to their contributions to cardiac dysfunction. Herein, we aimed to examine a possible role of Zn2+‐transporters in the development of heart failure (HF) via induction of ER stress. We first showed localizations of ZIP8, ZIP14 and ZnT8 to both sarcolemma and S(E)R in ventricular cardiomyocytes (H9c2 cells) using confocal together with calculated Pearson's coefficients. The expressions of ZIP14 and ZnT8 were significantly increased with decreased ZIP8 level in HF. Moreover, [Zn2+]i was significantly high in doxorubicin‐treated H9c2 cells compared to their controls. We found elevated levels of ER stress markers, GRP78 and CHOP/Gadd153, confirming the existence of ER stress. Furthermore, we measured markedly increased total PKC and PKCα expression and PKCα‐phosphorylation in HF. A PKC inhibition induced significant decrease in expressions of these ER stress markers compared to controls. Interestingly, direct increase in [Zn2+]i using zinc‐ionophore induced significant increase in these markers. On the other hand, when we induced ER stress directly with tunicamycin, we could not observe any effect on expression levels of these Zn2+ transporters. Additionally, increased [Zn2+]i could induce marked activation of PKCα. Moreover, we observed marked decrease in [Zn2+]i under PKC inhibition in H9c2 cells. Overall, our present data suggest possible role of Zn2+ transporters on an intersection pathway with increased [Zn2+]i and PKCα activation and induction of HF, most probably via development of ER stress. Therefore, our present data provide novel information how a well‐controlled [Zn2+]i via Zn2+ transporters and PKCα can be important therapeutic approach in prevention/treatment of HF. 相似文献
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Figen Amber Cicek Aysegul Toy Erkan Tuncay Belgin Can Belma Turan 《Journal of bioenergetics and biomembranes》2014,46(5):377-387
Current data support that pharmacological modulators of endoplasmic reticulum stress (ERS) have therapeutic potential for diabetic individuals. Therefore, we aimed to examine whether timolol, having free radical-scavenger action, besides being a β-blocker, exerts a cardioprotective effect via inhibition of ERS response in diabetic rats in a comparison with an antioxidant N-acetylcysteine (NAC). Histopathological data showed that either timolol- or NAC-treatment of diabetic rats prevented the changes in mitochondria and nucleus of the cardiac tissue while they enhanced the cellular redox-state in heart as well. The levels of ER-targeted cytoprotective chaperones GRP78 and calnexin, unfolded protein response signaling protein CHO/Gadd153 besides the levels of calpain, BCL-2, phospho-Akt, PUMA, and PML in the hearts from diabetic rats, treated with either timolol or NAC, are found to be similar among these groups, although all these parameters were markedly preserved in the untreated diabetics compared to those of the controls. Taken into consideration how important a balanced-ratio between anti-apoptotic and pro-apoptotic proteins for the maintenance mitochondria/ER function, our results suggest that ERS in diabetic rat heart is mediated by increased oxidative damage, which in turn triggers cardiac dysfunction. Moreover, we also demonstrated that timolol treatment of diabetic rats, similar to NAC treatment, induced a well-controlled redox-state and apoptosis in cardiac myocardium. We, thus for the first time, report that cardioprotective effect of timolol seems to be associated with normalization of ER function due to its antioxidant action in cardiomyocytes even under hyperglycemia. 相似文献
59.
Cicek Figen Amber Tokcaer-Keskin Zeynep Ozcinar Evren Bozkus Yusuf Akcali Kamil Can Turan Belma 《Molecular biology reports》2014,41(8):4853-4863
Metabolic syndrome (MetS) is a complex medical disorder characterized by insulin resistance, hypertension, and high risk of coronary disease and stroke. Microvascular rarefaction and endothelial dysfunction have also been linked with MetS, and recent evidence from clinical studies supports the efficacy of incretin-based antidiabetic therapies for vascular protection in diabetes. Previous studies pointed out the importance of dipeptidyl peptidase-4 (DPP-4) inhibition in endothelial cells due to getting protection against metabolic pathologies. We therefore aimed to investigate the acute effects of a DPP-4 inhibitor, sitagliptin, on vascular function in rats with high-sucrose diet-induced MetS. In order to elucidate the mechanisms implicated in the effects of DPP-4 inhibition, we tested the involvement of NO pathway and epigenetic regulation in the MetS. Acute use of sitagliptin protects the vascular function in the rats with MetS in part due to NO pathway via restoring the depressed aortic relaxation responses mediated by receptors. Application of sitagliptin enhanced the depressed phosphorylation levels of both the endothelial NO synthase and the apoptotic status of protein kinase B, known as Akt, in endothelium-intact thoracic aorta from rats with MetS. One-hour application of sitagliptin on aortic rings from rats with MetS also induced remarkable histon posttranslational modifications such as increased expression of H3K27Me3, but not of H3K27Me2, resulting in an accumulation of the H3K27Me3. Our findings suggest that, in addition to its well-known hypoglycemic action, sitagliptin may also have beneficial effects on hyperglycemia-induced vascular changes in an endotheium-dependent manner. These present results with sitagliptin aside from the glycaemic control, may demonstrate its important role in the treatment of patients with MetS. 相似文献
60.
Belma Skender Jiřina Hofmanová Josef Slavík Iva Jelínková Miroslav Machala Mary Pat Moyer Alois Kozubík Alena Hyršlová Vaculová 《Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids》2014,1841(9):1308-1317
Docosahexaenoic acid (DHA), an n-3 polyunsaturated fatty acid present in fish oil, may exert cytotoxic and/or cytostatic effects on colon cancer cells when applied individually or in combination with some anticancer drugs. Here we demonstrate a selective ability of subtoxic doses of DHA to enhance antiproliferative and apoptotic effects of clinically useful cytokine TRAIL (tumor necrosis factor-related apoptosis inducing ligand) in cancer but not normal human colon cells. DHA-mediated stimulation of TRAIL-induced apoptosis was associated with extensive engagement of mitochondrial pathway (Bax/Bak activation, drop of mitochondrial membrane potential, cytochrome c release), activation of endoplasmic reticulum stress response (CHOP upregulation, changes in PERK level), decrease of cellular inhibitor of apoptosis protein (XIAP, cIAP1) levels and significant changes in sphingolipid metabolism (intracellular levels of ceramides, hexosyl ceramides, sphingomyelines, sphingosines; HPLC/MS/MS). Interestingly, we found significant differences in representation of various classes of ceramides (especially C16:0, C24:1) between the cancer and normal colon cells treated with DHA and TRAIL, and suggested their potential role in the regulation of the cell response to the drug combination. These study outcomes highlight the potential of DHA for a new combination therapy with TRAIL for selective elimination of colon cancer cells via simultaneous targeting of multiple steps in apoptotic pathways. 相似文献