全文获取类型
收费全文 | 342篇 |
免费 | 29篇 |
出版年
2022年 | 5篇 |
2021年 | 8篇 |
2020年 | 5篇 |
2019年 | 2篇 |
2018年 | 5篇 |
2017年 | 11篇 |
2016年 | 9篇 |
2015年 | 10篇 |
2014年 | 10篇 |
2013年 | 26篇 |
2012年 | 22篇 |
2011年 | 21篇 |
2010年 | 14篇 |
2009年 | 16篇 |
2008年 | 12篇 |
2007年 | 16篇 |
2006年 | 15篇 |
2005年 | 14篇 |
2004年 | 16篇 |
2003年 | 13篇 |
2002年 | 9篇 |
2001年 | 11篇 |
2000年 | 9篇 |
1999年 | 6篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1994年 | 4篇 |
1993年 | 3篇 |
1991年 | 4篇 |
1990年 | 7篇 |
1988年 | 3篇 |
1987年 | 3篇 |
1985年 | 2篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1980年 | 3篇 |
1979年 | 10篇 |
1974年 | 3篇 |
1973年 | 3篇 |
1972年 | 2篇 |
1971年 | 3篇 |
1970年 | 2篇 |
1968年 | 3篇 |
1967年 | 4篇 |
1966年 | 2篇 |
1965年 | 1篇 |
1964年 | 4篇 |
1963年 | 1篇 |
1937年 | 1篇 |
排序方式: 共有371条查询结果,搜索用时 15 毫秒
121.
Gabriella M. Lawrence Shani Shulman Yechiel Friedlander Colleen M. Sitlani Ayala Burger Bella Savitsky Einat Granot‐Hershkovitz Thomas Lumley Pui‐Yan Kwok Stephanie Hesselson Daniel Enquobahrie Pandora L. Wander Orly Manor David S. Siscovick Hagit Hochner 《Obesity (Silver Spring, Md.)》2014,22(4):1165-1171
122.
123.
New micropaleontological data coming from three cores collected on the meso-Adriatic continental shelf (Vasto area) are studied. Comparisons to foraminiferal assemblages and radiocarbon dates previously collected from cores in the San Benedetto del Tronto and Tremiti areas allow the correlation of patterns observed in shallow water areas with those in deeper parts of the basin. We focused on the response of benthic foraminifera during the Holocene high-stand, corresponding to the installation of the recent sedimentary and trophic system. An influence of anthropogenic impacts cannot be ruled out; its effects consist of a depletion of oxygen level with a consequent modification of the structure of benthic foraminiferal assemblages. During the glacial/post glacial cycle, three phases, characterized by a total of six foraminiferal biofacies including different species assemblages were recognized. The first phase, from 14 kyr BP to 11 kyr BP, corresponds to the Bölling/Alleröd and Younger Dryas cold event, before the Holocene sea-level rise. During this phase, the continental shelf was characterized by an infralittoral environment with productive waters owing to the proximity of the Po river delta at the edge of the Mid-Adriatic Deep. The second phase, from 11 kyr BP to 4 kyr BP, represents the Holocene sea-level rise and is characterized by a condensed sedimentation spread over the entire basin. The third phase corresponds to the Holocene high-stand, during which time the modern current system became established. During this phase, the eastern portion of the shelf underwent to the central part of the mud-belt, corresponding to the sub-recent conditions. Recent eutrophication resulting from human activities over the last few centuries is evidenced by frequency fluctuations of typically opportunistic taxa such as Nonionella turgida and Epistominella vitrea. 相似文献
124.
Stefano Merler Marco Ajelli Barbara Camilloni Simona Puzelli Antonino Bella Maria Cristina Rota Alberto Eugenio Tozzi Maurizio Muraca Marcello Meledandri Anna Maria Iorio Isabella Donatelli Caterina Rizzo 《PloS one》2013,8(10)
Background
A common pattern emerging from several studies evaluating the impact of the 2009 A/H1N1 pandemic influenza (A/H1N1pdm) conducted in countries worldwide is the low attack rate observed in elderly compared to that observed in children and young adults. The biological or social mechanisms responsible for the observed age-specific risk of infection are still to be deeply investigated.Methods
The level of immunity against the A/H1N1pdm in pre and post pandemic sera was determined using left over sera taken for diagnostic purposes or routine ascertainment obtained from clinical laboratories. The antibody titres were measured by the haemagglutination inhibition (HI) assay. To investigate whether certain age groups had higher risk of infection the presence of protective antibody (≥1∶40), was calculated using exact binomial 95% CI on both pre- and post- pandemic serological data in the age groups considered. To estimate age-specific susceptibility to infection we used an age-structured SEIR model.Results
By comparing pre- and post-pandemic serological data in Italy we found age- specific attack rates similar to those observed in other countries. Cumulative attack rate at the end of the first A/H1N1pdm season in Italy was estimated to be 16.3% (95% CI 9.4%-23.1%). Modeling results allow ruling out the hypothesis that only age-specific characteristics of the contact network and levels of pre-pandemic immunity are responsible for the observed age-specific risk of infection. This means that age-specific susceptibility to infection, suspected to play an important role in the pandemic, was not only determined by pre-pandemic levels of H1N1pdm antibody measured by HI.Conclusions
Our results claim for new studies to better identify the biological mechanisms, which might have determined the observed pattern of susceptibility with age. Moreover, our results highlight the need to obtain early estimates of differential susceptibility with age in any future pandemics to obtain more reliable real time estimates of critical epidemiological parameters. 相似文献125.
126.
Matija Popovic Juraj Bella Ventsislav Zlatev Vesna Hodnik Gregor Anderluh Paul N. Barlow Alessandro Pintar Sándor Pongor 《Journal of molecular recognition : JMR》2011,24(2):245-253
Jagged‐1, one of the five Notch ligands in man, is a membrane‐spanning protein made of a large extracellular region and a 125‐residue cytoplasmic tail bearing a C‐terminal PDZ recognition motif (1213RMEYIV1218). Binding of Jagged‐1 intracellular region to the PDZ domain of afadin, a protein located at cell–cell adherens junctions, couples Notch signaling with the adhesion system and the cytoskeleton. Using NMR chemical shift perturbation and surface plasmon resonance, we studied the interaction between the PDZ domain of afadin (AF6_PDZ) and a series of polypeptides comprising the PDZ‐binding motif. Chemical shift mapping of AF6_PDZ upon binding of ligands of different length (6, 24, and 133 residues) showed that the interaction is strictly local and involves only the binding groove in the PDZ. The recombinant protein corresponding to the entire intracellular region of Jagged‐1, J1_ic, is mainly disordered in solution, and chemical shift mapping of J1_ic in the presence of AF6_PDZ showed that binding is not coupled to folding. Binding studies on a series of 24‐residue peptides phosphorylated at different positions showed that phosphorylation of the tyrosine at position ‐2 of the PDZ‐binding motif decreases its affinity for AF6_PDZ, and may play a role in the modulation of this interaction. Finally, we show that the R1213Q mutation located in the PDZ‐binding motif and associated with extrahepatic biliary atresia increases the affinity for AF6_PDZ, suggesting that this syndrome may arise from an imbalance in the coupling of Notch signaling to the cytoskeleton. Copyright © 2010 John Wiley & Sons, Ltd. 相似文献
127.
Xu WC Wright GE Brown NC Long ZY Zhi CX Dvoskin S Gambino JJ Barnes MH Butler MM 《Bioorganic & medicinal chemistry letters》2011,21(14):4197-4202
Several 2-anilino- and 2-benzylamino-3-deaza-6-oxopurines [3-deazaguanines] and selected 8-methyl and 8-aza analogs have been synthesized. 7-Substituted N2-(3-ethyl-4-methylphenyl)-3-deazaguanines were potent and selective inhibitors of Gram+ bacterial DNA polymerase (pol) IIIC, and 7-substituted N2-(3,4-dichlorobenzyl)-3-deazaguanines were potent inhibitors of both pol IIIC and pol IIIE from Gram+ bacteria, but weakly inhibited pol IIIE from Gram− bacteria. Potent enzyme inhibitors in both classes inhibited the growth of Gram+ bacteria (MICs 2.5-10 μg/ml), and were inactive against the Gram− organism Escherichia coli. Several derivatives had moderate protective activity in Staphylococcus aureus-infected mice. 相似文献
128.
129.
Hybrid systems composed of a glucose oxidase (GOx)/peroxidase-mimicking DNAzyme, and of microperoxidase-11 (MP-11)/anti-thrombin aptamer were synthesized. The hybrid systems were employed as amplifying labels for the colorimetric or chemiluminescence detection of an enzyme functions, and thrombin analysis, respectively. In the GOx/DNAzyme system, the GOx-mediated oxidation of glucose led to the formation of H(2)O(2), and this activated the oxidation of ABTS to a colored product, or to the generation of chemiluminescence in the presence of luminol. The MP-11/anti-thrombin aptamer enabled the amplified analysis of thrombin by the MP-11-mediated generation of chemiluminescence in the presence of luminol/H(2)O(2). 相似文献
130.