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61.
The study was a clinico-mycological approach to find out the various clinical types of dermatophytoses and as well as the species of dermatophytes in Goa. Various socio-demographic and host factors were assessed to determine the predisposing factors for dermatophytoses. A detailed clinical history of the patients was recorded. Samples were collected aseptically in sterile black paper envelopes. The material was subjected to microscopy using potassium hydroxide mount. Culture was done by inoculating the material in Sabouraud’s peptone glucose agar slants. Tinea corporis was found to be the most common clinical presentation (44.2 %). Among the dermatophytes, Trichophyton rubrum predominated (38.2 %), followed by Trichophyton mentagrophytes (27.2 %). Dermatophytoses was more common among the lower and upper lower strata of society. Majority of the patients were in the 21–40 years age group (62 %) with males predominating women in ratio 2.4:1. 相似文献
62.
T. Corrêa de Souza P. César Magalhães F. José Pereira E. Mauro de Castro S. Netto Parentoni 《Acta Physiologiae Plantarum》2011,33(5):1877-1885
This study was carried out to evaluate maize plants of different recurrent selection cycles of the variety (Zea mays L.—Saracura-BRS-4154) regarded to genetic gains, morphophysiology, and grain yield, achieved over the selection cycles under
intermittent flooding of the soil. This variety has the capacity to survive and produce in temporarily flooded soils and was
developed by the National Maize and Sorghum Research Center (EMBRAPA). The experiment was conducted in greenhouse by using
ten alternating selection cycles (Cycle 1–18) and BR 107 a variety known for its susceptibility to flooding. The flooding
initiated at six-leaf stage by applying 50 mm of water three times a week. At flowering, the following parameters were evaluated:
rate of leaf photosynthesis, stomatal conductance, intercellular CO2 concentration, transpiration rate, photosynthetically active leaf area, root porosity, relative chlorophyll content, grain
yield, harvest index, ear length, and interval between male and female flowering. Yield as a function of root porosity and
photosynthesis were also evaluated. An index was created in this study, in order to help the discussion of the characteristics
evaluated, it was called “Relative Tolerance Value—RTV”, only gaseous exchange measurements was not included in this index.
By the way, it was observed throughout the selection cycles an increase in all gaseous exchange parameters, being the cycle
18, the one which presented the greatest averages. RTV for leaf area showed the greatest values for cycles 7 and 18, whereas
root porosity, chlorophyll relative content, and harvest index, the greater RTV values were found in cycles 17 and 18. The
largest grain yield RTV was observed in cycle 7, followed by cycles 13, 15, and 18. Flooding resulted in longer Anthesis-Silking
Interval, especially for the first cycles. At flooding condition, grain yield was strongly related to root porosity (R
2 = 0.66). These results showed that the selection cycles of “Saracura” maize improved some morphophysiologic characters, which
favor their survival in flooded environments, also resulting in higher productivity. 相似文献
63.
Luis?Sánchez-PulidoEmail author Ana?M?Rojas Karel?H?van Wely Carlos?Martinez-A Alfonso?Valencia 《BMC bioinformatics》2004,5(1):91
Background
The Split ends (Spen) family are large proteins characterised by N-terminal RNA recognition motifs (RRMs) and a conserved SPOC (Spen paralog and ortholog C-terminal) domain. The aim of this study is to characterize the family at the sequence level. 相似文献64.
Marques MA Soares Ade S Pinto OW Barroso PT Pinto DP Ferreira-Filho M Werneck-Barroso E 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2007,852(1-2):308-316
A rapid and simple method for quantitation of metformin (MET) in human plasma by HPLC-MS/MS was developed and validated. The sample preparation consists of plasma deproteinization using acetonitrile. The mobile phase consisted of water-acetonitrile and formic acid (55/45/0.048, v/v/%) and the run time was 3 min. A pursuit C(18) (100 mm x 2.0 mm i.d., 3 microm) column connected to a guard column MS-pursuit (0.20 mm x 0.20 mm i.d., 5 microm) was used. The range of the calibration curve was from 20 to 5000 ng/mL, the limit of quantitation being 20 ng/mL. The detection was performed on a mass spectrometer (ESI+), using metoprolol as internal standard. The calibration curves have r(2) values of 0.995 (CV=0.24%, n=10). The accuracy and precision were between 90.74 and 106.7% and coefficients of variations (CV) of 1.10 and 4.35%, respectively. The method was applied to determine the pharmacokinetic parameters: C(max) (1667.25 ng/mL) and T(max) (3.89 h). 相似文献
65.
Baker MA Lane DJ Ly JD De Pinto V Lawen A 《The Journal of biological chemistry》2004,279(6):4811-4819
Porin isoform 1 or VDAC (voltage-dependent anion-selective channel) 1 is the predominant protein in the outer mitochondrial membrane. We demonstrated previously that a plasma membrane NADH-ferricyanide reductase activity becomes up-regulated upon mitochondrial perturbation, and therefore suggested that it functions as a cellular redox sensor. VDAC1 is known to be expressed in the plasma membrane; however, its function there remained a mystery. Here we show that VDAC1, when expressed in the plasma membrane, functions as a NADH-ferricyanide reductase. VDAC1 preparations purified from both plasma membrane and mitochondria fractions exhibit NADH-ferricyanide reductase activity, which can be immunoprecipitated with poly- and monoclonal antibodies directed against VDAC(1). Transfecting cells with pl-VDAC1-GFP, which carries an N-terminal signal peptide, directs VDAC1 to the plasma membrane, as shown by confocal microscopy and FACS analysis, and significantly increases the plasma membrane NADH-ferricyanide reductase activity of the transfected cells. This novel enzymatic activity of the well known VDAC1 molecule may provide an explanation for its role in the plasma membrane. Our data suggest that a major function of VDAC1 in the plasma membrane is that of a NADH(-ferricyanide) reductase that may be involved in the maintenance of cellular redox homeostasis. 相似文献
66.
Manuel B.?MoralesEmail author Vincent?Bretagnolle Beatriz?Arroyo 《Biodiversity and Conservation》2005,14(13):3135-3150
Stochastic computer simulations are used to evaluate the sensitivity of Little bustard population parameters, estimating the
survival probabilities of the seven endangered Little bustard populations of central-western France for which conservation
actions are currentlybeing or have been implemented. Different scenarios of parameter compensation for those nuclei to establish
parameter levels assuring population viability are discussed. Adult survival, productivity per female, initial population
size and carrying capacity were the most sensitive parameters in a hypothetical, isolated population. Juvenile survival also
affected population survival, although its sensitivity was lower. Sex ratio did not have a linear effect on population survival,
but probability of extinction increased for extreme values. Productivity per female and initial population size, varied strongly
among the populations studied, determining their average time of extinction and growth rate. When a metapopulation scenario
was simulated, the survival probabilities of each population and the metapopulations stayed close to 1.0 if no mortality was
associated to migration. When mortality during migration was included in the simulations, the metapopulation's probability
of survival significantly decreased under 90%. This approach may help managers to correctly address conservation measures
and design effective strategies, which should be directed mainly to improve productivity, enhance female survival, and minimise
mortality during migration (e.g. promoting insect-rich nesting substrates, avoiding female killing and nest destruction at
harvesting, reducing the risk of collision with powerlines, or controlling poaching). 相似文献
67.
de María PD Sánchez-Montero JM Alcántara AR Valero F Sinisterra JV 《Biotechnology letters》2005,27(7):499-503
Candida rugosa was cultured using different inducers (oleic acid, olive oil, sunflower oil, n-dodecanol and glycerol) as the only carbon source in batch conditions, as well as in several fed-batch fermentations (oleic acid as inducer) at variable feed rate conditions. The N-terminal analysis of each crude lipase revealed that, while the isoenzymes Lip2 and Lip3 are always secreted (at different proportions depending on the inducer), Lip1 was produced only using n-dodecanol (batch conditions) or oleic acid (fed-batch at high feed rate). The nature of the inducer controls the isoenzyme percentage; when this is fixed, as well as the feed rate in fed-batch fermentation, the isoenzymatic profile remained unaltered and the samples differed only in the activity of the lipases, as determined by heptyl oleate synthesis. 相似文献
68.
69.
Carla Malaquias Almeida Jos A. Manso Ana C. Figueiredo Liliana Antunes Rui Cruz Bruno Manadas Daniel Bur Pedro Jos Barbosa Pereira Carlos Faro Isaura Simes 《Applied microbiology and biotechnology》2017,101(18):6951-6968
The potential of using a synthetic cardosin-based rennet in cheese manufacturing was recently demonstrated with the development and optimization of production of a recombinant form of cardosin B in Kluyveromyces lactis. With the goal of providing a more detailed characterization of this rennet, we herein evaluate the impact of the plant-specific insert (PSI) on cardosin B secretion in this yeast, and provide a thorough analysis of the specificity requirements as well as the biochemical and structural properties of the isolated recombinant protease. We demonstrate that the PSI domain can be substituted by different linker sequences without substantially affecting protein secretion and milk clotting activity. However, the presence of small portions of the PSI results in dramatic reductions of secretion yields in this heterologous system. Kinetic characterization and specificity profiling results clearly suggest that synthetic cardosin B displays lower catalytic efficiency and is more sequence selective than native cardosin B. Elucidation of the structure of synthetic cardosin B confirms the canonical fold of an aspartic protease with the presence of two high mannose-type, N-linked glycan structures; however, there are some differences in the conformation of the flap region when compared to cardosin A. These subtle variations in catalytic properties and the more stringent substrate specificity of synthetic cardosin B help to explain the observed suitability of this rennet for cheese production. 相似文献
70.