全文获取类型
收费全文 | 799篇 |
免费 | 76篇 |
专业分类
875篇 |
出版年
2023年 | 5篇 |
2022年 | 13篇 |
2021年 | 27篇 |
2020年 | 19篇 |
2019年 | 20篇 |
2018年 | 12篇 |
2017年 | 19篇 |
2016年 | 27篇 |
2015年 | 50篇 |
2014年 | 48篇 |
2013年 | 58篇 |
2012年 | 65篇 |
2011年 | 58篇 |
2010年 | 28篇 |
2009年 | 28篇 |
2008年 | 43篇 |
2007年 | 34篇 |
2006年 | 43篇 |
2005年 | 48篇 |
2004年 | 36篇 |
2003年 | 36篇 |
2002年 | 36篇 |
2001年 | 11篇 |
2000年 | 13篇 |
1999年 | 17篇 |
1998年 | 5篇 |
1997年 | 4篇 |
1996年 | 6篇 |
1995年 | 4篇 |
1994年 | 8篇 |
1993年 | 4篇 |
1992年 | 2篇 |
1991年 | 7篇 |
1990年 | 6篇 |
1989年 | 4篇 |
1988年 | 2篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1984年 | 4篇 |
1983年 | 1篇 |
1981年 | 5篇 |
1980年 | 4篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1975年 | 1篇 |
1974年 | 3篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1956年 | 1篇 |
1955年 | 1篇 |
排序方式: 共有875条查询结果,搜索用时 0 毫秒
131.
The hemoglobin from the cyanobacterium Synechococcus sp. PCC 7002 (GlbN) contains three tyrosines (Tyr5, Tyr22, and Tyr53), each of which undergoes a structural rearrangement when the protein binds an exogenous ligand such as cyanide. We explored the use of 3-fluorotyrosine and (19) F-NMR spectroscopy for the characterization of GlbN. Assignment of (19) F resonances in fluorinated GlbN (GlbN*) was achieved with individual Tyr5Phe and Tyr53Phe replacements. We observed marked variations in chemical shift and linewidth reflecting the dependence of structural and dynamic properties on oxidation state, ligation state, and covalent attachment of the heme group. The isoelectronic complexes of ferric GlbN* with cyanide and ferrous GlbN* with carbon monoxide gave contrasting spectra, the latter exhibiting heterogeneity and enhanced internal motions on a microsecond-to-millisecond time scale. The strength of the H-bond network involving Tyr22 (B10) and bound cyanide was tested at high pH. 3-Fluorotyrosine at position 22 had a pK(a) value at least 3 units higher than its intrinsic value, 8.5. In addition, evidence was found for long-range communication among the tyrosine sites. These observations demonstrated the utility of the 3-fluorotyrosine approach to gain insight in hemoglobin properties. 相似文献
132.
Qian L Wei SJ Zhang D Hu X Xu Z Wilson B El-Benna J Hong JS Flood PM 《Journal of immunology (Baltimore, Md. : 1950)》2008,181(1):660-668
133.
134.
135.
Oleskowicz-Popiel P Lisiecki P Holm-Nielsen JB Thomsen AB Thomsen MH 《Bioresource technology》2008,99(13):5327-5334
In this communication, pretreatment of the anaerobically digested (AD) manure and the application of the pretreated AD manure as liquid medium for the simultaneous saccharification and fermentation (SSF) were described. Furthermore, fermentation of pretreated maize silage and wheat straw was investigated using 2 l bioreactors. Wet oxidation performed for 20 min at 121 °C was found as the most suitable pretreatment conditions for AD manure. High ammonia concentration and significant amount of macro- and micro-nutrients in the AD manure had a positive influence on the ethanol fermentation. No extra nitrogen source was needed in the fermentation broth. It was shown that the AD manure could successfully substitute process water in SSF of pretreated lignocellulosic fibres. Theoretical ethanol yields of 82% were achieved, giving 30.8 kg ethanol per 100 kg dry mass of maize silage. 相似文献
136.
Chiara Donfrancesco Simonetta Palleschi Luigi Palmieri Barbara Rossi Cinzia Lo Noce Fabio Pannozzo Belinda Spoto Giovanni Tripepi Carmine Zoccali Simona Giampaoli 《PloS one》2013,8(10)
Background
Chronic kidney disease (CKD) independently increases the risk of death and cardiovascular disease (CVD) in the general population. However, the relationship between estimated glomerular filtration rate (eGFR) and CVD/death risk in a general population at low risk of CVD has not been explored so far.Design
Baseline and longitudinal data of 1465 men and 1459 women aged 35-74 years participating to the MATISS study, an Italian general population cohort, were used to evaluate the role of eGFR in the prediction of all-cause mortality and incident CVD.Methods
Bio-bank stored sera were used to evaluate eGFR at baseline. Serum creatinine was measured on thawed samples by means of an IDMS-calibrated enzymatic method. eGFR was calculated by the CKD-EPI formula.Results
At baseline, less than 2% of enrolled persons had eGFR < 60 mL/min/1.73m2 and more than 70% had a 10-year cardiovascular risk score < 10%. In people 60 or more years old, the first and the last eGFR quintiles (<90 and ≥109 mL/min/1.73m2, respectively) were associated to an increased risk for both all-cause mortality (hazard ratio 1.6, 95% confidence interval 1.2-2.1 and 4.3, 1.6-11.7, respectively) and incident CVD (1.6, 1.0-2.4 and 7.0, 2.2-22.9, respectively), even if adjusted for classical risk factors.Conclusions
These findings strongly suggest that in an elderly, general population at low risk of CVD and low prevalence of reduced renal filtration, even a modest eGFR reduction is related to all-cause mortality and CVD incidence, underlying the potential benefit to this population of considering eGFR for their risk prediction. 相似文献137.
Tregoning JS Yamaguchi Y Wang B Mihm D Harker JA Bushell ES Zheng M Liao G Peltz G Openshaw PJ 《Journal of immunology (Baltimore, Md. : 1950)》2010,185(9):5384-5391
Respiratory syncytial virus (RSV) is a major cause of respiratory morbidity, resulting in hospitalization for bronchiolitis in some infected infants that is associated with wheeze in later life. Genetic factors are known to affect the severity of the sequelae after RSV infection, but the complexity of the temporal and genetic effects makes it difficult to analyze this response in studies in man. Therefore, we developed a murine genetic model to analyze the sequelae occurring after RSV infection in early life. Haplotype-based genetic analysis of interstrain differences in severity identified the MHC as an important genetic determinant. This was confirmed by analysis of responses in congenic mice with different MHC haplotypes. We also found that susceptible strains had high CD8 levels during secondary infection. Analysis of first filial generation, second filial generation, and back-cross progeny produced by intercrossing resistant (H-2(k), C3H/HeN) and sensitive (H-2(b), BALB/c) strains indicated that susceptibility to sequelae after RSV infection was dominantly inherited but also segregated in a non-MHC-dependent manner. Thus, MHC haplotype and its effect on CD8 cell response is an important determinant of the outcome of neonatal RSV infection. 相似文献
138.
139.
Alcione Silva de Carvalho Kelly Salom?o Solange Lisboa de Castro Taline Ramos Conde Helena Pereira da Silva Zamith Ernesto Raúl Caffarena Belinda Suzette Hall Shane Robert Wilkinson Núbia Boechat 《Memórias do Instituto Oswaldo Cruz》2014,109(3):315-323
Megazol (7) is a 5-nitroimidazole that is highly active against Trypanosomacruzi and Trypanosoma brucei, as well as drug-resistantforms of trypanosomiasis. Compound 7 is not used clinically due to its mutagenic andgenotoxic properties, but has been largely used as a lead compound. Here, we comparedthe activity of 7 with its 4H-1,2,4-triazole bioisostere (8) inbloodstream forms of T. brucei and T. cruzi andevaluated their activation by T. brucei type I nitroreductase(TbNTR) enzyme. We also analysed the cytotoxic and genotoxiceffects of these compounds in whole human blood using Comet and fluoresceindiacetate/ethidium bromide assays. Although the only difference between 7 and 8 isthe substitution of sulphur (in the thiadiazole in 7) for nitrogen (in the triazolein 8), the results indicated that 8 had poorer antiparasitic activity than 7 and wasnot genotoxic, whereas 7 presented this effect. The determination of Vmax indicatedthat although 8 was metabolised more rapidly than 7, it bounds to theTbNTR with better affinity, resulting in equivalent kcat/KMvalues. Docking assays of 7 and 8 performed within the active site of a homologymodel of the TbNTR indicating that 8 had greater affinity than7. 相似文献
140.