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101.
PHIL M. NOVIS DAVID WHITEHEAD ED G. GREGORICH† JOHN E. HUNT ASHLEY D. SPARROW‡§ DAVID W. HOPKINS¶ BO ELBERLING LAURIE G. GREENFIELD‡ 《Global Change Biology》2007,13(6):1224-1237
Nostoc commune Vaucher (a cyanobacterium) is a very conspicuous terrestrial primary producer in Victoria Land, continental Antarctica. Because polar ecosystems are considered to be especially sensitive to environmental changes, understanding the environmental constraints on net carbon (C) fixation by N. commune is necessary to determine the effects of environmental changes on the ecological functioning of ice‐free areas of the continent. A model describing net C fixation in terrestrial populations of N. commune in an Antarctic dry valley was constructed using field and laboratory measurements in which N. commune colonies were exposed to different combinations of incident irradiance (400–700 nm), temperature, and degree of desiccation. For desiccated N. commune mats with water content ≤ 30% saturation, net C fixation was highly variable between replicates and could not be modelled. However, for colonies at > 30% saturation, rates of net C fixation and dark respiration depended strongly on irradiance and temperature. Net C fixation reached a maximum rate of 21.6 μg C m− 2 s− 1 at irradiance of approximately 250 μmol m− 2 s− 1 and the optimum temperature of 20.5 °C. Agreement between predicted short‐term net C fixation and field and laboratory measurements allowed estimation of total seasonal fixation, using previously published environmental data. Annual net C fixation was estimated in the range 14.5–21.0 g C fixed m− 2Nostoc mat, depending on year/season. Estimates for different seasons correlated with thermal time (accumulated hours above 0 °C during the year) rather than irradiance, in contrast to communities in local lacustrine environments, where irradiance is the main driver of primary productivity. In the terrestrial habitat, N. commune appears to compromise between an ability to capitalize on short periods of higher temperature and efficient utilization of lower irradiance at low temperature. The relationship between thermal time and net annual C fixation by N. commune is strongly linear. 相似文献
102.
Methanococcus deltae (Delta)LH was grown on H(inf2)-CO(inf2) in the presence of various concentrations of furfural. Furfural at higher concentrations, namely, 20 and 25 mM, inhibited growth of this organism. At concentration of 5 and 10 mM, no inhibition of growth was observed. The other methanogens in this study were not inhibited by 10 mM furfural. Among the methanogens tested, M. deltae was capable of transforming furfural, whereas Methanobacterium thermoautotrophicum Marburg, Methanosarcina barkeri 227, Methanococcus thermolithotrophicus, and Methanobrevibacter ruminantium lacked this capability. One hundred percent removal of furfural was observed within 48 h of incubation in M. deltae cultures. The end product observed during furfural metabolism was furfuryl alcohol. An almost stoichiometric amount of furfuryl alcohol was produced by M. deltae. This transformation is likely to be of value in the detoxification of furfural and in its ultimate conversion to methane and CO(inf2) by anaerobic digestion. 相似文献
103.
Genetic adaptation to high altitude in the Ethiopian highlands 总被引:1,自引:0,他引:1
Scheinfeldt LB Soi S Thompson S Ranciaro A Woldemeskel D Beggs W Lambert C Jarvis JP Abate D Belay G Tishkoff SA 《Genome biology》2012,13(1):R1-9
Background
Genomic analysis of high-altitude populations residing in the Andes and Tibet has revealed several candidate loci for involvement in high-altitude adaptation, a subset of which have also been shown to be associated with hemoglobin levels, including EPAS1, EGLN1, and PPARA, which play a role in the HIF-1 pathway. Here, we have extended this work to high- and low-altitude populations living in Ethiopia, for which we have measured hemoglobin levels. We genotyped the Illumina 1M SNP array and employed several genome-wide scans for selection and targeted association with hemoglobin levels to identify genes that play a role in adaptation to high altitude.Results
We have identified a set of candidate genes for positive selection in our high-altitude population sample, demonstrated significantly different hemoglobin levels between high- and low-altitude Ethiopians and have identified a subset of candidate genes for selection, several of which also show suggestive associations with hemoglobin levels.Conclusions
We highlight several candidate genes for involvement in high-altitude adaptation in Ethiopia, including CBARA1, VAV3, ARNT2 and THRB. Although most of these genes have not been identified in previous studies of high-altitude Tibetan or Andean population samples, two of these genes (THRB and ARNT2) play a role in the HIF-1 pathway, a pathway implicated in previous work reported in Tibetan and Andean studies. These combined results suggest that adaptation to high altitude arose independently due to convergent evolution in high-altitude Amhara populations in Ethiopia. 相似文献104.
105.
The composition and annual cycle of the zooplankton of two Ethiopian Rift Valley soda lakes is described. Lake Langano has a conductivity of 1 400 to 1800 µS cm–1 and a permanent mineral turbidity. Lake Abiata is more concentrated (conductivity 19 000 to 23 000 µS cm–1) and more alkaline but less turbid; it is characterised by dense phytoplankton blooms, mainly cyanophytes.The zooplankton assemblage is typically tropical, with relatively few species of Cladocera and Copepoda. There was a marked difference in zooplankton between the two lakes, Lake Abiata showing much higher concentrations and greater wet season/ dry season differences. The species composition was also different. Lake Abiata lacked Cladocera, and calanoid copepods occurred only during the wet season with lower conductivities. These two phenomena were attributed to high sodium bicarbonate concentration and to dense cyanophyte blooms. Eleven species of rotifers occurred in Lake Abiata, including six Brachionus spp. but B. rubens was the only rotifer found in Lake Langano. The seasonal variation of the zooplankton is discussed in relation to seasonal fluctuations in conductivity and Chl a concentration. 相似文献
106.
Current knowledge on potential health benefits of Spirulina 总被引:2,自引:0,他引:2
Amha Belay Yoshimichi Ota Kazuyuki Miyakawa Hidenori Shimamatsu 《Journal of applied phycology》1993,5(2):235-241
Spirulina is a microscopic filamentous alga that is rich in proteins, vitamins, essential amino acids, minerals and essential fatty
acids like γ-linolenic acid (GLA). It is produced commercially and sold as a food supplement in health food stores around
the world. Up to very recently, the interest in Spirulina was mainly in its nutritive value. Currently, however, numerous people are looking into the possible therapeutic effects
of Spirulina. Many pre-clinical studies and a few clinical studies suggest several therapeutic effects ranging from reduction of cholesterol
and cancer to enhancing the immune system, increasing intestinal lactobacilli, reducing nephrotoxicity by heavy metals and
drugs and radiation protection. This paper presents a critical review of some published and unpublished data on therapeutic
effects of Spirulina. 相似文献
107.
Spirulina has been used as human food supplement for over 20 years, but its use as an animal feed supplement is relatively recent. This paper reviews earlier studies on its more established effects on growth, survival and tissue quality in a whole range of animals and more recent studies on its immunomodulatory, anti-viral and anti-cancer effects. The basis for potential application as an animal feed supplement is discussed. 相似文献
108.
Seasonal variation in phytoplankton primary production in relation to light and nutrients in Lake Awasa,Ethiopia 总被引:1,自引:1,他引:0
The biomass and primary production of phytoplankton in Lake Awasa, Ethiopia was measured over a 14 month period, November 1983 to March 1985. The lake had a mean phytoplankton biomass of 34 mg chl a m–3 (n = 14). The seasonal variation in phytoplankton biomass of the euphotic zone (mg chl a m–2 h–1) was muted with a CV (standard deviation/mean) of 31%. The vertical distribution of photosynthetic activity was of a typical pattern for phytoplankton with light inhibition on all but overcast days. The maximum specific rates of photosynthesis or photosynthetic capacity (Ømax) for the lake approached 19 mg O2 (mg chl a)–1 h–1, with high values during periods of low phytoplankton biomass. Areal rates of photosynthesis ranged between 0.30 to 0.73 g O2 m–2 h–1 and 3.3 to 7.8 g O2 m–2 d–1. The efficiency of utilisation of PhAR incident on the lake surface varied from 2.4 to 4.1 mmol E–1 with the highest efficiency observed corresponding to the lowest surface radiation. Calculated on a caloric basis, the efficiency ranged between 1.7 and 2.9%. The temporal pattern of primary production by phytoplankton showed limited variability (CV = 21 %). 相似文献
109.
D E Robertson M F Roberts N Belay K O Stetter D R Boone 《Applied and environmental microbiology》1990,56(5):1504-1508
The unusual compound beta-aminoglutaric acid (beta-glutamate) has been identified by 13C nuclear magnetic resonance spectroscopy in soluble extracts of marine methanogenic bacteria. We examined several methanogen species representing nine genera and found that beta-glutamate occurred in methanococci and two methanogenium strains (Methanogenium cariaci JR1 and "Methanogenium anulus" AN9). The presence of this compound in the methanococci examined was further restricted to thermophilic members of the genus Methanococcus, including Methanococcus thermolithotrophicus strains, Methanococcus jannaschii, and "Methanococcus igneus." The two Methanogenium strains examined were mesophiles. Studies using Methanococcus thermolithotrophicus showed that levels of beta-glutamate in cells of that species were not affected by variation in growth temperature (40 to 65 degrees C), NH4+ (2 to 80 mM), Mg2+ (10 to 50 mM), or K+ (2 to 10 mM) in the medium. In contrast, soluble pools of beta-glutamate and L-alpha-glutamate (the other major free amino acid in all the methanococci) were proportional to NaCl levels in the growth medium. This dependence of beta-glutamate and L-alpha-glutamate concentrations on salt levels in the medium suggests that they function as osmolytes in these cells. 相似文献
110.
Traction force on a kinetochore at metaphase acts as a linear function of kinetochore fiber length 总被引:16,自引:14,他引:2 下载免费PDF全文
We are investigating the relation between the force pulling a kinetochore poleward and the length of the corresponding kinetochore fiber. It was recognized by Ostergren in 1950 (Hereditas 36:1-19) that the metaphase position of a chromosome could be achieved by a balance of traction forces were proportional to the distance from kinetochore to pole. For the typical chromosome (i.e., a meiotic bivalent or mitotic chromosome) with a single kinetochore fiber extending to each pole, the resultant force (RF) would equal zero when the chromosome lay at the midpoint between the two poles. For special chromosomes that have unequal numbers of kinetochore fibers extending towards opposite poles. For special chromosomes that have unequal numbers of kinetochore fibers extending towards opposite poles. For special chromosomes that have unequal numbers of kinetochore fibers extending towards opposite poles, Ostergren’s proposal suggests that RF = 0 when the chromosome is shifted closer to the pole toward which the greater number of kinetochore fibers are pulling. We have measured the force-length relationship in living spindles by analyzing the metaphase positions of experimentally generated multivalent chromosomes having three or four kinetochore fibers. Multivalent chromosomes of varied configurations were generated by γ-irradiation of nymphs of the grasshopper melanoplus differentialis, and their behavior was analyzed in living first meiotic spermocytes. The lengths of kinetochore fibers were determined from time-lapse photographs by measuring the kinetochore-to-pole distances for fully congressed chromosomes just before the onset of anaphase. In our analysis, force (F) along a single kinetochore fiber is expressed by: F = kL(exp), where k is a length-independent proportionality constant, L represents the kinetochore fiber length, and exp is an unknown exponent. The RF on a chromosome is then given by: RF = σk(i)L(i)(exp), where kinetochore fiber lengths in opposite half- spindles are given opposite sign. If forces on a metaphase chromosome are at equilibrium (RF = 0), then for asymmetrical orientations of multivalents we can measure the individual kinetochore fiber lengths (L(i)) and solve for the exponent that yields a resultant force of zero. The value of the exponent relates how the magnitude of force along a kinetochore fiber varies with its length. For six trivalents and one naturally occurring quadrivalent we calculated an average value of exp = 1.06 +/- 0.18. This result is consistent with Ostergren’s hypothesis and indicates that the magnitude of poleward traction force along a kinetochore fiber is directly proportional to the length of the fiber. Our finding suggests that the balance of forces along a kinetochore fiber may be a major factor regulating the extent of kinetochore microtubule assembly. 相似文献