四川省鸡形目鸟类及分布

研究使用了从1997~2005年四川省58个生物资源综合科学考察的鸟类野外调查记录、国内外从1908年到2005年有关四川省鸡形目鸟类野外物种调查公开发表的60篇文献,以及四川大学、西华师范大学、四川农业大学标本馆共386号鸡形目标本,共1103条记录,对四川省鸡形目的资源及分布进行了研究。结果如下:①四川省鸡形目鸟类计2科18属25种,占全国鸡形目(63种)的39.68%,其中,国家I级保护鸡形目5种,国家II级保护鸡形目11种,分别占全国保护鸡形目总数(I级21种、II级21种)的23.81%、52.38%;特有种7种,占全国鸡形目特有种(14种)的50%,分布型以喜马拉雅-横断山区型居多,以东洋界成分占绝对优势。②物种丰富度最高的地区为平武县、松潘县、九寨沟县、绵竹县、冕宁县、康定县、青川县和宝兴县,保护物种丰富度最高的地区为平武县、九寨沟县、宝兴县、松潘县、青川县、冕宁县;③四川省159个县(市)中有鸡形目调查记录的有73个,没有记录的县86个,这86个空白区域有待进一步调查。     

高职药学类专业《微生物学》课程教学体系优化

围绕教学材料、教学内容、教学方法、教学手段、教学师资、考核机制等6个层面,详细阐述了高职药学类专业《微生物学》课程的教学体系优化问题,为课程教学改革提供一定参考依据。     

春季和秋季莓叶委陵菜叶片和地下部分芦丁及儿茶素含量的HPLC分析

Contents of rutin and catechin in leaves and under-ground part of Potentilla fragarioides L.in spring and autumn were determined by HPLC technology.The results show that in spring,rutin content in leaves and under-ground part of P.fragarioides is 1.670 3 and 0.054 4 mg·g-1,and catechin content is 0.263 5 and 0.723 5 mg·g-1,respectively.In autumn,rutin content is 0.586 9 and 0.010 7 mg·g-1,and catechin content is 0.784 3 and 0.366 4 mg·g-1,respectively.The rutin content in leaves is the highest in spring,and catechin content in leaves is the highest in autumn.Therefore,collecting leaves in spring is suitable for taking rutin as a purpose,while collecting leaves in autumn is suitable for taking catechin as a purpose.     

Inhibition of AMPK expression in skeletal muscle by systemic inflammation in COPD rats

Background

Analysis of exhaled breath condensates (EBC) is a non-invasive technique to evaluate biomarkers such as antioxidants in the pediatric population, but limited data exists of its use in intubated patients, particularly newborns. Currently, tracheal aspirate (TA) serves as the gold standard collection modality in critically ill newborns, but this method remains invasive. We tested the hypothesis that glutathione status would positively correlate between EBC and TA collections in intubated newborns in the Newborn Intensive Care Unit (NICU). We also hypothesized that these measurements would be associated with alveolar macrophage (AM) glutathione status in the newborn lung.

Methods

Reduced glutathione (rGSH), glutathione disulfide (GSSG), and total GSH (rGSH + (2 X GSSG)) were measured in sequential EBC and TA samples from 26 intubated newborns via high performance liquid chromatography (HPLC). Additionally, AM glutathione was evaluated via immunofluorescence. Pearson’s correlation coefficient and associated 95% confidence intervals were used to quantify the associations between raw and urea-corrected concentrations in EBC and TA samples and AM staining. Statistical significance was defined as p ≤ 0.05 using two-tailed tests. The sample size was projected to allow for a correlation coefficient of 0.5, with 0.8 power and alpha of 0.05.

Results

EBC was obtainable from intubated newborns without adverse clinical events. EBC samples demonstrated moderate to strong positive correlations with TA samples in terms of rGSH, GSSG and total GSH. Positive correlations between the two sampling sites were observed in both raw and urea-corrected concentrations of rGSH, GSSG and total GSH. AM glutathione staining moderately correlated with GSSG and total GSH status in both the TA and EBC.

Conclusions

GSH status in EBC samples of intubated newborns significantly correlated with the GSH status of the TA sample and was reflective of cellular GSH status in this cohort of neonatal patients. Non-invasive EBC sampling of intubated newborns holds promise for monitoring antioxidant status such as GSH in the premature lung. Further studies are necessary to evaluate the potential relationships between EBC biomarkers in the intubated premature newborn and respiratory morbidities.     

溃疡性结肠炎与缺血性结肠炎临床特征内镜特点相关分析

目的：提高缺血性结肠炎（IC）和与溃疡性结肠炎（UC）诊断的正确率。方法：选择2008年1月至2011年6月的住院患者,确诊IC 43例,UC 36例,对其临床特征、内镜特点进行回顾性比较分析。结果：组间性别、年龄、病程和基础疾病比较差异有统计学意义（P〈0.05）,而主要临床表现比较,差异无统计学意义。IC组以老年女性多见,病程短,多伴发心脑血管等基础疾病（29/43,67.0%）。IC组C反应蛋白高于UC组,血小板低于UC组（P〈0.05）。IC多累及乙状结肠,直肠少见;病变为区域局限性,溃疡为纵形或不规则形,病灶愈合迅速,病理以黏膜炎症为主。UC组病变多起源于直肠,一般累及肠壁全周,病变为倒灌连续性,以散在针尖样地图状溃疡为主,病理表现为隐窝炎和脓肿。结论：年龄、病程、基础疾病、C反应蛋白、内镜及病理特征是鉴别诊断的要点。     

疏水修饰聚阳离子高分子SP-Chol在COS-7细胞中转染和毒性的研究

目的：研究以精胺为单体,以乙二醇二氯甲酸酯作为连接剂,以胆固醇氯甲酸酯作为疏水基团连接剂合成的疏水修饰聚阳离子高分子SP-Chol对非洲绿猴肾癌细胞COS-7的转染活性和细胞毒性的影响。方法：以荧光素酶质粒为报告基因,研究SP-Chol与DNA的复合物在COS-7细胞的转染活性,用MTT方法研究SP-Chol对COS-7细胞的毒性。结果：COS-7细胞实验显示,SP-Chol具有低于PEI 25kDa的细胞毒性,同时也具有高效输送DNA的能力。结论：SP-Chol是一种新型的高效、低毒,在基因治疗领域有潜在应用价值的非病毒基因输送载体。     

静电纺丝纳米纤维体外释放与蛋白活性的研究

目的:通过选择不同的模型蛋白,探讨准确的研究静电纺丝纳米纤维支架的体外释放和快速的测定蛋白活性的方法.方法:通过O/W乳液法静电纺丝制备纳米纤维,并用扫描电镜对纳米纤维表面进行了表征.以GM-CSF为模型蛋白,采用ELISA双抗体夹心法考察纤维的体外释放行为;以BSA为模型蛋白,用SEC-H-PLC比较纤维制备前后蛋白的聚集情况;以β-半乳糖苷酶为模型蛋白,用ONPG法比较纤维制备前后酶的催化活性.结果:纤维表面平滑,直径均一,呈现互相连通的三维网状结构.纤维在5天内释放90％以上;纤维中回收的BSA单体比例为66.53％;β-半乳糖苷酶在纤维中的催化活性保持原活性的3.37％.结论:通过选择不同的模型蛋白,能够准确的测定静电纺丝纤维的体外释放,快速的考察纤维中的蛋白活性,对于更好的研究蛋白药物纳米纤维支架具有重要的参考价值.     

四川九顶山自然保护区周边社区经济及环境意识调查和建议

应用参与式乡村评估方法(PRA)对四川九顶山自然保护区周边社区5个乡镇的经济状况及居民的环境意识进行了调查,结果表明:(1)居民的主要经济来源是经济林木产品和矿山务工收入,对森林资源的依赖性不强;(2)当地大量的采矿业破坏了自然环境;(3)在调查居民的发展愿望时,37%的人认为应大力发展种植业,由于社区经济发展不平衡,居民的发展愿望差异明显;(4)各乡镇居民对保护区的认识和环境保护的看法有显著差异,经济发展地区的环境意识较强;(5)保护区对居民的宣传教育偏少.保护区应加大对居民的宣教工作,积极开展社区共管;同时也应注意到经济发展的滞后可能影响社区的环境保护工作的开展,所以在推动保护区的工作同时,应实现当地经济的可持续发展.     

变铅青链霉菌异柠檬酸脱氢酶的异源表达及序列分析

通过同源性引物成功扩增和克隆了变铅青链霉菌(Streptomyces lividans)TK54的异柠檬酸脱氢酶(isocitrate dehydrogenase, IDH) (简称SlIDH)基因icd (GenBank登录号为EU661252).icd的起始密码子为GTG,GC含量为69.55 %,显示了链霉菌基因的高GC含量特征,实现了SlIDH在E.coli中的异源高效表达.0.5 mmol/L的IPTG为最佳诱导条件.SlIDH的分子量约为80 kD.在Mn~(2+)或Mg~(2+)条件下,SlIDH以NADP~+为辅酶时的活性分别为7.94 U/mg及4.00 U/mg,以NAD~+为辅酶时的活性分别为0.58 U/mg及0.27 U/mg,SlIDH更偏爱以NADP~+为辅酶.与不同种属单体IDH的氨基酸序列比对显示,SlIDH与单体IDH的序列一致性均在60 %以上.因此本工作首次以实验性证据初步鉴定了SlIDH为NADP-依赖型单体IDH.本工作为进一步探索单体IDH的结构与功能以及单体IDH与同源二聚体IDH的进化关系奠定了基础.