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991.
Trees of genus Populus (in our context primarily poplars) are predominantly grown in Sweden in small plantations on arable land in southern and central parts of the country to produce biomass for energy and other purposes. This study evaluated the effects (i) of poplar plantations on groundwater quality, by determining differences in leaching of nitrogen and phosphorus to groundwater, and (ii) of poplar and hybrid aspen plantations on soil quality in terms of carbon in the top- and subsoil. The study was conducted comparing Populus plantations in Sweden with adjacent fields with cereals and grasslands. The experiment concerning the groundwater leaching was conducted in eight poplar plantations along three growing seasons (2012–2015). For the soil carbon experiments, 19 poplar and two hybrid aspen plantations and the respective reference fields were sampled. NO3-N leaching from poplar plantations was significantly lower than that from reference fields with cereals, but not when compared with grasslands. Spring NO3-N leaching was significantly lower in poplars than in the reference fields, whereas leaching of NO3-N in autumn did not differ. Concentrations of PO4-P in the groundwater of poplar plantations were lower compared to the respective ones of the reference fields. There were no clear trends observed when comparing carbon concentrations in the topsoil of the poplar and hybrid aspen plantations compared to the respective adjacent reference fields. For the subsoil, the average carbon concentrations in the poplar and hybrid aspen plantations were equal to the respective ones of cereals, but were higher when compared to grassland. 相似文献
992.
993.
Zhongkai Wang Jingwen Song Liang Zhang Songqun Huang Lizhi Bao Feng Chen Xianxian Zhao 《Cell stress & chaperones》2017,22(2):245-252
Alcohol abuse is a risk factor for a distinct form of congestive heart failure, known as alcoholic cardiomyopathy (ACM). Here, we investigate how microRNAs may participate in the induction of cardiomyocyte apoptosis associated with ethanol exposure in vitro. Increasing the concentrations of ethanol to primary rat cardiomyocytes resulted in elevated apoptosis assessed by annexin V and propidium iodide staining, and reduced expression of an enzyme for alcohol detoxification aldehyde dehydrogenase 2 (ALDH2). These ethanol effects were accompanied by a substantial elevation of miR-378a-5p. Driving miR-378a-5p overexpression in cardiomyocytes decreased ALDH2. The specific interaction of miR-378a-5p with the 3’UTR of ALDH2 was examined by luciferase reporter assays, and we found that miR-378a-5p activity depends on a complementary base pairing at the 3′-UTR region of ALDH2 mRNA. Finally, ethanol-induced apoptosis in cardiomyocytes was attenuated in the presence of anti-miR378a-5p. Collectively, these data implicate a likely involvement of miR-378a-5p in the stimulation of cardiomyocyte apoptosis through ALDH2 gene suppression, which might play a potential role in the pathogenesis of ACM. 相似文献
994.
Lijun Gao Hongfang Wang Zhenguo Liu Shuchang Liu Guangdong Zhao Baohua Xu Xingqi Guo 《Cell stress & chaperones》2017,22(6):867-877
Serine proteinases play important roles in innate immunity and insect development. We isolated a serine proteinase gene, designated AccSp10, from the Chinese honeybees (Apis cerana cerana). RT-qPCR and a Western blot analysis at different pupal development stages indicated that AccSp10 might be involved in melanin formation in pupae and promote pupal development. In adult workers, the expression of AccSp10 was upregulated by treatments mimicking harmful environments such as the presence of Bacillus bombysepticus, different temperatures (4, 24 and 42 °C), HgCl2, H2O2 and paraquat; the exception was treatment with VC (vitamin C), which did not upregulate AccSp10 expression. Western blot confirmed the results. A disc diffusion assay indicated that recombinant AccSp10 accelerated E. coli cell death during stimulation with harmful substances (HgCl2, paraquat and cumene hydroperoxide). These findings suggest that AccSp10 may be involved in the pupal development of Chinese honeybees and protection against microorganisms and abiotic harms. 相似文献
995.
Kshirod K. Jena Sherry Lou Hechanova Holden Verdeprado G. D. Prahalada Sung-Ryul Kim 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2017,130(11):2345-2360
Key message
A first set of 25 NILs carrying ten BPH resistance genes and their pyramids was developed in the background of indica variety IR24 for insect resistance breeding in rice.Abstract
Brown planthopper (Nilaparvata lugens Stal.) is one of the most destructive insect pests in rice. Development of near-isogenic lines (NILs) is an important strategy for genetic analysis of brown planthopper (BPH) resistance (R) genes and their deployment against diverse BPH populations. A set of 25 NILs with 9 single R genes and 16 multiple R gene combinations consisting of 11 two-gene pyramids and 5 three-gene pyramids in the genetic background of the susceptible indica rice cultivar IR24 was developed through marker-assisted selection. The linked DNA markers for each of the R genes were used for foreground selection and confirming the introgressed regions of the BPH R genes. Modified seed box screening and feeding rate of BPH were used to evaluate the spectrum of resistance. BPH reaction of each of the NILs carrying different single genes was variable at the antibiosis level with the four BPH populations of the Philippines. The NILs with two- to three-pyramided genes showed a stronger level of antibiosis (49.3–99.0%) against BPH populations compared with NILs with a single R gene NILs (42.0–83.5%) and IR24 (10.0%). Background genotyping by high-density SNPs markers revealed that most of the chromosome regions of the NILs (BC3F5) had IR24 genome recovery of 82.0–94.2%. Six major agronomic data of the NILs showed a phenotypically comparable agronomic performance with IR24. These newly developed NILs will be useful as new genetic resources for BPH resistance breeding and are valuable sources of genes in monitoring against the emerging BPH biotypes in different rice-growing countries.996.
Changyou Liu Jing Wu Lanfen Wang Baojie Fan Zhimin Cao Qiuzhu Su Zhixiao Zhang Yan Wang Jing Tian Shumin Wang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2017,130(11):2375-2393
Key message
A novel genetic linkage map was constructed using SSR markers and stable QTLs were identified for six drought tolerance related-traits using single-environment analysis under irrigation and drought treatments.Abstract
Mungbean (Vigna radiata L.) is one of the most important leguminous food crops. However, mungbean production is seriously constrained by drought. Isolation of drought-responsive genetic elements and marker-assisted selection breeding will benefit from the detection of quantitative trait locus (QTLs) for traits related to drought tolerance. In this study, we developed a full-coverage genetic linkage map based on simple sequence repeat (SSR) markers using a recombinant inbred line (RIL) population derived from an intra-specific cross between two drought-resistant varieties. This novel map was anchored with 313 markers. The total map length was 1010.18 cM across 11 linkage groups, covering the entire genome of mungbean with a saturation of one marker every 3.23 cM. We subsequently detected 58 QTLs for plant height (PH), maximum leaf area (MLA), biomass (BM), relative water content, days to first flowering, and seed yield (Yield) and 5 for the drought tolerance index of 3 traits in irrigated and drought environments at 2 locations. Thirty-eight of these QTLs were consistently detected two or more times at similar linkage positions. Notably, qPH5A and qMLA2A were consistently identified in marker intervals from GMES5773 to MUS128 in LG05 and from Mchr11-34 to the HAAS_VR_1812 region in LG02 in four environments, contributing 6.40–20.06% and 6.97–7.94% of the observed phenotypic variation, respectively. None of these QTLs shared loci with previously identified drought-related loci from mungbean. The results of these analyses might facilitate the isolation of drought-related genes and help to clarify the mechanism of drought tolerance in mungbean.997.
Naeela Qureshi Harbans Bariana Kerrie Forrest Matthew Hayden Beat Keller Thomas Wicker Justin Faris Elena Salina Urmil Bansal 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2017,130(3):495-504
Key message
Fine mapping of Yr47 and Lr52 in chromosome arm 5BS of wheat identified close linkage of the marker sun180 to both genes and its robustness for marker-assisted selection was demonstrated.Abstract
The widely effective and genetically linked rust resistance genes Yr47 and Lr52 have previously been mapped in the short arm of chromosome 5B in two F3 populations (Aus28183/Aus27229 and Aus28187/Aus27229). The Aus28183/Aus27229 F3 population was advanced to generate an F6 recombinant inbred line (RIL) population to identify markers closely linked with Yr47 and Lr52. Diverse genomic resources including flow-sorted chromosome survey sequence contigs representing the orthologous region in Brachypodium distachyon, the physical map of chromosome arm 5BS, expressed sequence tags (ESTs) located in the 5BS6-0.81-1.00 deletion bin and resistance gene analog contigs of chromosome arm 5BS were used to develop markers to saturate the target region. Selective genotyping was also performed using the iSelect 90 K Infinium wheat SNP assay. A set of SSR, STS, gene-based and SNP markers were developed and genotyped on the Aus28183/Aus27229 RIL population. Yr47 and Lr52 are genetically distinct genes that mapped 0.4 cM apart in the RIL population. The SSR marker sun180 co-segregated with Lr52 and mapped 0.4 cM distal to Yr47. In a high resolution mapping population of 600 F2 genotypes Yr47 and Lr52 mapped 0.2 cM apart and marker sun180 was placed 0.4 cM distal to Lr52. The amplification of a different sun180 amplicon (195 bp) than that linked with Yr47 and Lr52 (200 bp) in 204 diverse wheat genotypes demonstrated its robustness for marker-assisted selection of these genes.998.
Annaliese S. Mason Erin E. Higgins Rod J. Snowdon Jacqueline Batley Anna Stein Christian Werner Isobel A. P. Parkin 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2017,130(4):621-633
The Brassica napus 60K Illumina Infinium? SNP array has had huge international uptake in the rapeseed community due to the revolutionary speed of acquisition and ease of analysis of this high-throughput genotyping data, particularly when coupled with the newly available reference genome sequence. However, further utilization of this valuable resource can be optimized by better understanding the promises and pitfalls of SNP arrays. We outline how best to analyze Brassica SNP marker array data for diverse applications, including linkage and association mapping, genetic diversity and genomic introgression studies. We present data on which SNPs are locus-specific in winter, semi-winter and spring B. napus germplasm pools, rather than amplifying both an A-genome and a C-genome locus or multiple loci. Common issues that arise when analyzing array data will be discussed, particularly those unique to SNP markers and how to deal with these for practical applications in Brassica breeding applications. 相似文献
999.
Na Zhang Xiaoli Fan Fa Cui Chunhua Zhao Wei Zhang Xueqiang Zhao Lijuan Yang Ruiqing Pan Mei Chen Jie Han Jun Ji Dongcheng Liu Zongwu Zhao Yiping Tong Aimin Zhang Tao Wang Junming Li 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2017,130(6):1235-1252
Key message
The temporal and spatial expression patterns of stable QTL for plant height and their influences on yield were characterized.Abstract
Plant height (PH) is a complex trait in wheat (Triticum aestivum L.) that includes the spike length (SL) and the internode lengths from the first to the fifth internode, which are counted from the top and abbreviated as FIRITL, SECITL, THIITL, FOUITL, and FIFITL, respectively. This study identified eight putative additive quantitative trait loci (QTL) for PH. In addition, unconditional and conditional QTL mapping were used to analyze the temporal and spatial expression patterns of five stable QTL for PH. qPh-3A mainly regulated SL, FIRITL, and FIFITL to affect PH during the booting–heading stage (BS–HS); qPh-3D regulated all internode lengths to affect PH, especially during the BS–HS; before HS, qPh-4B mainly affected FIRITL, SECITL, THIITL, and FOUITL and qPh-5A.1 mainly affected SECITL, THIITL, and FOUITL to regulate PH; and qPh-6B mainly regulated FIRITL to affect the PH after the booting stage (BS). qPhdv-4B, a QTL for the response of PH to nitrogen stress, was stable and co-localized with qPh-4B. All five stable QTL, except for qPh-3A, were related to the 1000 kernel weight and yield per plant. Regions of qPh-3A, qPh-3D, qPh-4B, qPh-5A.1, and qPh-6B showed synteny to parts of rice chromosomes 1, 1, 3, 9, and 2, respectively. Based on comparative genomics analysis, Rht-B1b was cloned and mapped in the CI of qPh-4B. This report provides useful information for fine mapping of the stable QTL for PH and the genetic improvement of wheat plant type.1000.
Baohua Wang Xavier Draye Zhimin Zhuang Zhengsheng Zhang Min Liu Edward L. Lubbers Don Jones O. Lloyd May Andrew H. Paterson Peng W. Chee 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2017,130(6):1297-1308