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131.
Fazelnia Kambiz Fakhraei Jafar Yarahmadi Hossein Mansoori Amini Kumarss 《Probiotics and antimicrobial proteins》2021,13(4):1081-1092
Probiotics and Antimicrobial Proteins - This study evaluates the effects of probiotics and synbiotics on the performance, immune responses, and intestinal morphology, and the expression of... 相似文献
132.
Prashanth Kenchappa Virender S Sangwan Niyaz Ahmed KRajender Rao Avinash Pathengay Annie Mathai Tarannum Mansoori Taraprasad Das Seyed E Hasnain Savitri Sharma 《Annals of clinical microbiology and antimicrobials》2005,4(1):1-8
Background
The number of Salmonella strains with reduced susceptibility to fluoroquinolones has increased during recent years in many countries, threatening the value of this antimicrobial group in the treatment of severe salmonella infections.Methods
We analyzed the in vitro activities of ciprofloxacin and 10 additional fluoroquinolones against 816 Salmonella strains collected from Finnish patients between 1995 and 2003. Special attention was focused on the efficacy of newer fluoroquinolones against the Salmonella strains with reduced ciprofloxacin susceptibility.Results
The isolates represented 119 different serotypes. Of all 816 Salmonella strains, 3 (0.4%) were resistant to ciprofloxacin (MIC ≥ 4 μg/ml), 232 (28.4%) showed reduced susceptibility to ciprofloxacin (MIC ≥ 0.125 – 2 μg/ml), and 581 (71.2%) were ciprofloxacin-susceptible. The MIC50 and MIC90 values of ciprofloxacin for these strains were 0.032 and 0.25 μg/ml, respectively, being lower than those of the other fluoroquinolone compounds presently on market in Finland (ofloxacin, norfloxacin, levofloxacin, and moxifloxacin). For two newer quinolones, clinafloxacin and sitafloxacin, the MIC50 and MIC90 values were lowest, both 0.016 and 0.064 μg/ml, respectively. Moreover, clinafloxacin and sitafloxacin exhibited the lowest MIC50 and MIC90 values, 0.064 and 0.125 μg/ml, against the 235 Salmonella strains with reduced susceptibility and strains fully resistant to ciprofloxacin.Conclusion
Among the registered fluoroquinolones in Finland, ciprofloxacin still appears to be the most effective drug for the treatment salmonella infections. Among the newer preparations, both clinafloxacin and sitafloxacin are promising based on in vitro studies, especially for strains showing reduced ciprofloxacin susceptibility. Their efficacy, however, has not been demonstrated in clinical investigations. 相似文献133.
Hashemipour M Faghihimani S Zolfaghary B Hovsepian S Ahmadi F Haghighi S 《Hormone research》2004,62(6):278-282
OBJECTIVE: To determine the prevalence of clinical polycystic ovary syndrome (PCOS) in 14- to 18-year-old high school girls in Isfahan, Iran. METHODS: In this cross-sectional study, 1,000 high school girls (14-18 years old) were selected by multi-stage random sampling from different high schools in Isfahan. Following physical examination, a single physician recorded the presence of hirsutism, severe acne, androgenic alopecia, menstrual dysfunction and obesity using a validated questionnaire. Clinical PCOS was diagnosed if menstrual dysfunction and clinical hyperandrogenism were detected. RESULTS: Clinical PCOS was present in 30 (3%), hirsutism in 60 (6%), menstrual dysfunction in 74 (7.4%) and severe acne in 47 (4.7%) of the population studied. CONCLUSION: The prevalence of clinical PCOS in our study population was similar to those of other studies; however, the prevalence could have been higher with hormonal assessment. 相似文献
134.
Behzad Salehi Mansour Bayat Mehrouz Dezfulian Azar Sabokbar Bahman Tabaraie 《Saudi Journal of Biological Sciences》2018,25(6):1236-1241
Fungal polysaccharides are well-known for the medicinal properties such as antitumor and immunomodulating effects. Hence, this study evaluated antitumor effects of polysaccharide extracted from Fusarium sp. isolated from soil samples of Karaj district, Alborz, Iran along with its taxonomic study. The filamentous fungus strain FK1 was isolated from the soil sample of Karaj, Iran. The strain was identified based on cultural, morphological and 18 S rRNA gene parameters as Fusarium. Further, the strain Fusarium was cultured in fermented broth of modified (PDB) for 10 days at 25 °C. The polysaccharide of strain FK1 was extracted from the mycelium free supernatant by boiling water method and evaluated for antitoxicity effect on two human cancer cell lines: HeLa cell line and Lymphoblastoid cell line (LCL) by MTT method. Findings revealed that water-extracted from mycelia polysaccharide of strain FK1 had the highest cytotoxicity effect against LCL which is the cause of B lymphocyte cancer, at 50 μg/ml concentration dose (114 ± 1.63) followed by 100 μg/ml (105 ± 0.57) and 10 μg/ml (104 ± 0.57), while it did not have a considerable effect on HeLa cell line. Fusarium could be alternative sources as an antitumor component. 相似文献
135.
Atieh Makhlough Soroosh Shekarchian Reza Moghadasali Behzad Einollahi Mona Dastgheib Ghasem Janbabaee Seyedeh Esmat Hosseini Nasrin Falah Fateme Abbasi Hossein Baharvand Nasser Aghdami 《Cytotherapy》2018,20(5):660-669
Background
Chronic kidney disease (CKD) is a progressive loss of kidney function and structure that affects approximately 13% of the population worldwide. A recent meta-analysis revealed that cell-based therapies improve impaired renal function and structure in preclinical models of CKD. We assessed the safety and tolerability of bone marrow–mesenchymal stromal cell (MSC) infusion in patients with CKD.Methods
A single-arm study was carried out at one center with 18-month follow-up in seven eligible patients with CKD due to different etiologies such as hypertension, nephrotic syndrome (NS) and unknown etiology. We administered an intravenous infusion (1–2?×?106 cells/kg) of autologous cultured MSCs. The primary endpoint was safety, which was measured by number and severity of adverse events. The secondary endpoint was decrease in the rate of decrease in estimated glomerular filtration rate (eGFR). We compared kidney function during the follow-up visits to baseline and 18 months prior to the intervention.Results
Follow-up visits of all seven patients were completed; however, we have not observed any cell-related adverse events during the trial. Changes in eGFR (P?=?0.10) and serum creatinine (P?=?0.24) from 18 months before cell infusion to baseline in comparison with baseline to 18 months were not statistically significant.Conclusions
We showed safety and tolerability of a single-dose infusion of autologous MSCs in patients with CKD. 相似文献136.
Behzad Fathinia Nasrullah Rastegar-Pouyani Hamid Darvishnia Arya Shafaeipour Ghodrat Jaafari 《Zoology in the Middle East.》2016,62(3):247-254
Using the stomach flushing technique, a total of 188 specimens of the Marsh Frog, Pelophylax ridibundus, were flushed, of which 129 had at least one food item in the stomach. The diet consisted of Annelida, Mollusca, Arthropoda and Chordata with Arthropoda being the most abundant group. Both diet volume and number of food items per stomach were significantly larger in April than in August. The proportion of frequency of occurrence (FOi%) of food categories did not show a constant food item in flushed materials. Pelophylax ridibundus prefers Diptera, Coleoptera, Amphipoda and Hymenoptera over other food categories. The Index of Relative Importance (IRI) for food categories differs between sexes and seasons. Differences were found in the food volume and the number of food items between seasons, but not between sexes. Prey volume is positively correlated with frog size. 相似文献
137.
Hamed?Ebrahimzadeh Mehran?E.?ShariatpanahiEmail author Behzad?Ahmadi Hassan?Soltanloo Mahmoud?Lotfi Eissa?Zarifi 《Journal of Plant Growth Regulation》2018,37(4):1127-1134
In this study, the effect of spraying mother plants with various levels of putrescine, spermidine, and cycocel (each at 0, 50, 500, and 5000 mg/l) were assessed on the frequency of haploid embryos produced from unfertilized ovaries and subsequent regeneration of derived embryos. Significantly higher haploid embryos were obtained when mother plants were sprayed with putrescine at 500 mg/l (5.2 embryos/fruit), spermidine at 50 mg/l (4.8 embryos/fruit), and cycocel at 50 mg/l (5.2 embryos/fruit) as compared to the control (without spraying, 3.2 embryos/fruit). However, embryogenesis induction was decreased drastically as the concentration of all the three compounds tested was increased and the lowest haploid embryos were observed when 5000 mg/l of spermidine (0.4 embryos/fruit) or cycocel (2.0 embryos/fruit) were applied. Only spermidine at 50 mg/l led to 100% regeneration into fully developed plantlets. The seed setting and size of fruits were also affected by polyamines and cycocel applications. Ploidy analysis using a flow cytometer indicated that all regenerated plantlets contain the gametic chromosome number (n?=?x?=?7) of parental plants and the results of chromosome counting also confirmed the haploid nature of regenerated plantlets. It can be concluded that the induction of haploid embryogenesis from unfertilized ovaries after pollination with irradiated pollen and subsequent conversion of derived embryos into the plantlets could be improved in Cucumis sativus L. by applying appropriate levels of putrescine, spermidine, and cycocel. 相似文献
138.
139.
Nezhadasad Behzad Radjabian Tayebeh Hajiboland Roghieh 《Journal of plant research》2023,136(1):117-137
Journal of Plant Research - Tolerance mechanisms employed by plants under environmental stresses can protect them against other co-occurring stresses. In this study, the effect of pre-exposure and... 相似文献
140.
David Conchouso Amani Al-Ma'abadi Hayedeh Behzad Mohammed Alarawi Masahito Hosokawa Yohei Nishikawa Haruko Takeyama Katsuhiko Mineta Takashi Gojobori 《基因组蛋白质组与生物信息学报(英文版)》2021,19(3):504-518
Droplet microfluidic techniques have shown promising outcome to study single cells at high throughput. However, their adoption in laboratories studying"-omics"sciences is still irrelevant due to the complex and multi-disciplinary nature of the field. To facilitate their use, here we provide engineering details and organized protocols for integrating three droplet-based microfluidic technologies into the metagenomic pipeline to enable functional screening of bioproducts at high throughput. First, a device encapsulating single cells in droplets at a rate of~ 250 Hz is described considering droplet size and cell growth. Then, we expand on previously reported fluorescence-activated droplet sorting systems to integrate the use of 4 independent fluorescence-exciting lasers (i.e., 405, 488, 561, and 637 nm) in a single platform to make it compatible with different fluorescence-emitting biosensors. For this sorter, both hardware and software are provided and optimized for effortlessly sorting droplets at 60 Hz. Then, a passive droplet merger is also integrated into our pipeline to enable adding new reagents to already-made droplets at a rate of 200 Hz. Finally, we provide an optimized recipe for manufacturing these chips using silicon dry-etching tools. Because of the overall integration and the technical details presented here, our approach allows biologists to quickly use microfluidic technologies and achieve both single-cell resolution and high-throughput capability (>50,000 cells/day) for mining and bioprospecting metagenomic data. 相似文献