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121.
For economic and environmental reasons, chickens with superior feed efficiency (FE) are preferred in the broiler chicken industry. High FE (HFE) chickens typically have reduced abdominal fat, the major adipose tissue in chickens. In addition to its function of energy storage, adipose tissue is a metabolically active organ that also possesses endocrine and immune regulatory functions. It plays a central role in maintaining energy homeostasis. Comprehensive understanding of the gene expression in the adipose tissue and the biological basis of FE are of significance to optimize selection and breeding strategies. Through gene expression profiling of abdominal fat from high and low FE (LFE) commercial broiler chickens, the present study aimed to characterize the differences of gene expression between HFE and LFE chickens. mRNA-seq analysis was carried out on the total RNA of abdominal fat from 10 HFE and 12 LFE commercial broiler chickens, and 1.48 billion of 75-base sequence reads were generated in total. On average, 11,565 genes were expressed (>5 reads/gene/sample) in the abdominal fat tissue, of which 286 genes were differentially expressed (DE) at q (False Discover Rate) < 0.05 and fold change > 1.3 between HFE and LFE chickens. Expression levels from RNA-seq were confirmed with the NanoString nCounter analysis system. Functional analysis showed that the DE genes were significantly (p < 0.01) enriched in lipid metabolism, coagulation, and immune regulation pathways. Specifically, the LFE chickens had higher expression of lipid synthesis genes and lower expression of triglyceride hydrolysis and cholesterol transport genes. In conclusion, our study reveals the overall differences of gene expression in the abdominal fat from HFE and LFE chickens, and the results suggest that the divergent expression of lipid metabolism genes represents the major differences. 相似文献
122.
Background
Both genome-wide association (GWA) studies and genomic selection depend on the level of non-random association of alleles at different loci, i.e. linkage disequilibrium (LD), across the genome. Therefore, characterizing LD is of fundamental importance to implement both approaches. In this study, using a 60K single nucleotide polymorphism (SNP) panel, we estimated LD and haplotype structure in crossbred broiler chickens and their component pure lines (one male and two female lines) and calculated the consistency of LD between these populations.Results
The average level of LD (measured by r2) between adjacent SNPs across the chicken autosomes studied here ranged from 0.34 to 0.40 in the pure lines but was only 0.24 in the crossbred populations, with 28.4% of adjacent SNP pairs having an r2 higher than 0.3. Compared with the pure lines, the crossbred populations consistently showed a lower level of LD, smaller haploblock sizes and lower haplotype homozygosity on macro-, intermediate and micro-chromosomes. Furthermore, correlations of LD between markers at short distances (0 to 10 kb) were high between crossbred and pure lines (0.83 to 0.94).Conclusions
Our results suggest that using crossbred populations instead of pure lines can be advantageous for high-resolution QTL (quantitative trait loci) mapping in GWA studies and to achieve good persistence of accuracy of genomic breeding values over generations in genomic selection. These results also provide useful information for the design and implementation of GWA studies and genomic selection using crossbred populations.Electronic supplementary material
The online version of this article (doi:10.1186/s12711-015-0098-4) contains supplementary material, which is available to authorized users. 相似文献123.
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Ahmadzadeh M Behnam S Sharifi Tehrani A Hedjaroude GA 《Communications in agricultural and applied biological sciences》2007,72(4):957-960
Biological control of sclerotinia disease, as an important alternative to chemical control, has received considerable attention due to the lack of resistant varieties in most crop, and increasing concerns over fungicide resistance in population of Sclerotinia sclerotiorum, and fungicide residues in the environment. One biocontrol agent, Pseudomonas fluorescens PB-3, has been showed the antagonistic relationship between itself and S. sclerotiorum was investigated in this study. A petal infection technique was used to detect efficacy of timing of application strain PB-3 in the suppression of S. sclerotiorum on canola. Significant difference in disease severity (p<0.05) were found with respect to timing of ascospore applications in the control treatments (ascospores only). The superior competitive ability strain PB-3 was demonstrated by its complete suppression of disease severity when applied as a co-inoculation treatment or prior to ascospores inoculation. Analysis of effect of applying strain PB-3 after ascospores was indicated that treatment in which strain PB-3 was added to petals 48 or 24 h after ascospores, or when there were no bacteria present at all, had higher rates of disease progression. It would be appear that bacteria are able to significantly inhibit disease when applied before or even at the same time as ascospores. In a practical sense, this could mean that a field application of antagonist could be concurrent with infection by the pathogen. 相似文献
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Behnam Zolghadr Bernhard Gasselhuber Markus Windwarder Martin Pabst Daniel Kracher Martina Kerndl Sonja Zayni Andreas Hofinger-Horvath Roland Ludwig Dietmar Haltrich Chris Oostenbrink Christian Obinger Paul Kosma Paul Messner Christina Schäffer 《Extremophiles : life under extreme conditions》2015,19(2):451-467
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Analysis of petroleum biodesulfurization in an airlift bioreactor using response surface methodology
Irani ZA Mehrnia MR Yazdian F Soheily M Mohebali G Rasekh B 《Bioresource technology》2011,102(22):10585-10591
For the first time, growing cells of Gordonia alkanivorans RIPI90A were used for biodesulfurization (BDS) of diesel. This process was carried out in an internal airlift bioreactor. BDS parameters (oil/water phase ratio and initial sulfur concentration) were optimized in flasks using response surface methodology. Predicted results were found to be in good agreement with experimental results. Initial sulfur concentration had a remarkable effect on BDS process. Maximum removal of sulfur (21 mg/l) can be achieved at oil/water phase ratio of 25% (v/v) and initial sulfur concentration of 28 mg/l. Moreover, effect of superficial gas velocity (Ug) and working volume (v) on volumetric gas liquid mass transfer coefficient was studied in an airlift bioreactor for BDS of diesel. The best results were achieved at Ug and v of 2.5l/min and 6.6l, respectively. Subsequently, BDS of diesel was investigated in an airlift bioreactor under optimized conditions. Sulfur reduction after 30 h was 14 mg/l. 相似文献
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