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91.
Genetic diversity for traits such as fibre quality or disease resistance to microorganisms is limited in the elite cotton germplasm; consequently, cotton breeders are looking for novel alleles in the secondary or even in the tertiary gene pools. The wild Australian Gossypium species (tertiary gene pool) represent an alternative source of novel alleles. However, to use these species efficiently, enabling tools are required. Chromosome-specific molecular markers are particularly useful tools to track the transmission of this exotic genetic material into the cultivated cotton during introgression. In this study, we report the construction of a genetic linkage map of the Australian wild C-genome species Gossypium sturtianum. The map, based on an F(2) population of 114 individuals, contains 291 AFLP loci. The map spans 1697 cM with an average distance of 5.8 cM between markers. To associate C-genome chromosomes with the A and D subgenomes of cultivated cotton, 29 SSR and RFLP-STS markers were assigned to chromosomes using cultivated cotton mapped marker information. Polymorphisms were revealed by 51 AFLP primer combinations and 38 RFLP-STS and 115 SSR cotton mapped markers. The utility of transferring RFLP-STS and SSR cotton mapped markers to other Gossypium species shows the usefulness of a comparative approach as a source of markers and for aligning the genetic map of G. sturtianum with the cultivated species in the future. This also indicates that the overall structure of the G. sturtianum linkage groups is similar to that of the A and D subgenomes of cotton at the gross structural level. Applications of the map for the Australia wild C-genome species and cotton breeding are discussed. 相似文献
92.
93.
The arbuscular mycorrhizal fungal (AMF) communities from the Yungas forests of Argentina were studied. The AMF species present in the rhizosphere of some dominant native plants (one tree: Alnus acuminata; three herbaceous species: Duchesnea indica, Oxalis conorrhiza, Trifolium aff. repens; and one shrub: Sambucus peruviana) from two sites (Quebrada del Portugués and Narváez Range) of the Yungas forests were isolated, identified and quantified during the four seasons of the year. Twenty-two AMF morphotaxa were found. Spore density of some AMF species at each site varied among seasons. The genera that most contributed to the biodiversity index were Acaulospora for Quebrada del Portugués and Glomus for Narváez Range. High diversity values were observed in the Yungas forests, particularly in the spring (rainy season). We concluded AMF differed in species composition and seasonal sporulation dynamics in the Yungas forests. 相似文献
94.
The morphological boundaries between Arbutus xalapensis and A. glandulosa are diffuse. Many individuals share traits of both species. The character most commonly used to distinguish the species is the presence of glandular hairs in A. glandulosa. Comparisons in the field of the number of colonies and level of defoliation by Eucheira socialis (Lepidoptera, Pieridae), an Arbutus-specific herbivore, showed that the damage is greater for glabrous than pilose trees and greater for pilose than glandular trees. Additionally, the geographic distribution of glandular trees is strongly correlated with that of E. socialis while that of non-glandular trees is not. We suggest that the glandular and non-glandular forms could belong to one single polymorphic species in which the glandular characteristic is maintained by differential herbivory. 相似文献
95.
This is the first iconographic taxonomic database intended as an everyday tool for the practising taxonomist, that allows
access to the original drawings used to describe species. We demonstrate the system with water mites (Acari, Parasitengona,
Hydrachnidia) but it may be used with any other plant or animal group. Query by genus or species leads to the original drawings
and any additional images associated to that particular name. At present, the database includes images from more than 4,000
species (around 38,000 illustrations) and is easily expandable. Copyright does not allow the database to be used in an open
WWW context, but it can be an extremely useful tool in private taxonomic applications. 相似文献
96.
Renée L. Eriksen José L. Hierro ?zkan Eren Krikor Andonian Katalin T?r?k Pablo I. Becerra Daniel Montesinos Liana Khetsuriani Alecu Diaconu Rick Kesseli 《PloS one》2014,9(12)
The natural history of introduced species is often unclear due to a lack of historical records. Even when historical information is readily available, important factors of the invasions such as genetic bottlenecks, hybridization, historical relationships among populations and adaptive changes are left unknown. In this study, we developed a set of nuclear, simple sequence repeat markers and used these to characterize the genetic diversity and population structure among native (Eurasian) and non-native (North and South American) populations of Centaurea solstitialis L., (yellow starthistle). We used these data to test hypotheses about the invasion pathways of the species that were based on historical and geographical records, and we make inferences about historical relationships among populations and demographic processes following invasion. We confirm that the center of diversity and the native range of the species is likely the eastern Mediterranean region in the vicinity of Turkey. From this region, the species likely proceeded to colonize other parts of Europe and Asia via a slow, stepwise range expansion. Spanish populations were the primary source of seed to invade South America via human-mediated events, as was evident from historical records, but populations from the eastern Mediterranean region were also important. North American populations were largely derived from South America, but had secondary contributors. We suggest that the introduction history of non-native populations from disparate parts of the native range have allowed not just one, but multiple opportunities first in South America then again in North America for the creation of novel genotypes via intraspecific hybridization. We propose that multiple intraspecific hybridization events may have created especially potent conditions for the selection of a noxious invader, and may explain differences in genetic patterns among North and South America populations, inferred differences in demographic processes, as well as morphological differences previously reported from common garden experiments. 相似文献
97.
Till JH Becerra M Watty A Lu Y Ma Y Neubert TA Burden SJ Hubbard SR 《Structure (London, England : 1993)》2002,10(9):1187-1196
Muscle-specific kinase (MuSK) is a receptor tyrosine kinase expressed selectively in skeletal muscle. During neuromuscular synapse formation, agrin released from motor neurons stimulates MuSK autophosphorylation in the kinase activation loop and in the juxtamembrane region, leading to clustering of acetylcholine receptors. We have determined the crystal structure of the cytoplasmic domain of unphosphorylated MuSK at 2.05 A resolution. The structure reveals an autoinhibited kinase domain in which the activation loop obstructs ATP and substrate binding. Steady-state kinetic analysis demonstrates that autophosphorylation results in a 200-fold increase in k(cat) and a 10-fold decrease in the K(m) for ATP. These studies provide a molecular basis for understanding the regulation of MuSK catalytic activity and suggest that an additional in vivo component may contribute to regulation via the juxtamembrane region. 相似文献
98.
Barish GD Yu RT Karunasiri MS Becerra D Kim J Tseng TW Tai LJ Leblanc M Diehl C Cerchietti L Miller YI Witztum JL Melnick AM Dent AL Tangirala RK Evans RM 《Cell metabolism》2012,15(4):554-562
Highlights? Bcl6 is a critical antiatherogenic regulator ? Inhibition of Bcl6-SMRT/NCoR interactions induces atherogenic gene expression ? SMRT and NCoR cistromes each exceed 30,000 sites, with a nearly 50% overlap ? Bcl6 recruits SMRT and NCoR to NF-κB-driven inflammatory and tissue remodeling genes 相似文献
99.
José A Andrades Shirley C Motaung Pedro Jiménez-Palomo Silvia Claros José M López-Puerta José Becerra Thomas M Schmid A Hari Reddi 《Arthritis research & therapy》2012,14(2):R72-8
Introduction
Articular cartilage (AC) is an avascular tissue with precise polarity and organization. The three distinct zones are: surface, middle and deep. The production and accumulation of the superficial zone protein (SZP), also known as lubricin, by the surface zone is a characteristic feature of AC. To date, there is a wealth of evidence showing differentiation of AC from mesenchymal stem cells. Most studies that described chondrogenic differentiation did not focus on AC with characteristic surface marker SZP/lubricin. The present investigation was initiated to determine the induction of SZP/lubricin in skeletal muscle-derived mesenchymal stem/progenitor cells (MDMSCs) by transforming growth factor-β1 (TGF-β1) and bone morphogenetic protein-7 (BMP-7).Methods
MDMSCs were cultured as a monolayer at a density of 1 × 105 cells/well in 12-well tissue culture plates. Cell cultures were treated for 3, 7 and 10 days with TGF-β1 and BMP-7. The medium was analyzed for SZP. The cells were used to isolate RNA for RT-PCR assays for SZP expression.Results
The SZP/lubricin increased in a time-dependent manner on Days 3, 7 and 10 in the medium. As early as Day 3, there was a three-fold increase in response to 3 ng/ml of TGF-β1 and 300 ng/ml of BMP-7. This was confirmed by immunochemical localization of SZP as early as Day 3 after treatment with TGF-β1. The expression of SZP mRNA was enhanced by TGF-β1.Conclusions
The present investigation demonstrated the efficient and reproducible induction of SZP/lubricin accumulation by TGF-β1 and BMP-7 in skeletal MDMSCs. Optimization of the experimental conditions may permit the utility of MDMSCs in generating surface zone-like cells with phenotypic markers of AC and, therefore, constitute a promising cell source for tissue engineering approaches of superficial zone cartilage. 相似文献100.
Darwin suggested that all life on Earth could be phylogenetically related. Modern biology has confirmed Darwin??s extraordinary insight; the existence of a universal genetic code is just one of many evidences of our common ancestry. Based on the three domain phylogeny proposed by Woese and Fox in the early 1970s that all living beings can be classified on one of three main cellular lineages (Archaea, Bacteria, and Eukarya), it is possible to reconstruct some of the characteristics of the Last Universal Common Ancestor or cenancestor. Comparative genomics of organisms from the three domains has shown that the cenancestor was not a direct descendant of the prebiotic soup nor a primitive cellular entity where the genotype and the phenotype had an imprecise relationship (i.e., a progenote), rather it was an organism similar in complexity to extant cells. Due to the process of horizontal gene transfer and secondary gene losses, several questions regarding the nature of the cenancestor remain unsolved. However, attempts to infer its nature have led to the identification of a set of universally conserved genes. The research on the nature of the last universal common ancestor promises to shed light on fundamental aspects of living beings. 相似文献