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981.
Beatriz H. Ramírez Rocío Cortés-B Olga Patricia Pinzón Laura Gómez Santiago Jacquin Eduardo Hernández Luz Angélica Quimbayo Raúl Giovanni Bogotá-A 《Biotropica》2023,55(2):489-503
Tropical montane cloud forests (TMCF) in the Orinoco River Basin are vulnerable to climate and regional land-use changes. These changes will force TMCF to migrate upwards, affecting biodiversity conservation and water flow regulation. Here, we evaluate how vegetation and soil macrofauna composition vary along the hydrometeorological gradient driven by an increase in fog incidence with elevation. Vegetation data were collected for all individuals with a diameter at breast height (DBH) > 5 cm in four vegetation plots (5 × 50 m; total: 0.1 ha) every 100 m in altitude between 1700 and 2200 m a.s.l. From each plot, we obtained three soil monoliths from the organic layer and three from the mineral horizon, and manually extracted their soil macrofauna. For these groups, we describe: (1) their compositional changes along the hydrometeorological gradient employing ordination analyses techniques and (2) the relation of the composition changes between vegetation and soil macrofauna communities using a symmetrical co-correspondence analysis. Our results show that the vegetation morphospecies composition and soil macrofauna-order composition vary significantly with the hydrometeorological gradient along elevation. The co-correspondence between vegetation and soil macrofauna reveals a shared breakpoint above the 2000 m a.s.l., where fog is more persistent. Furthermore, we identified eight indicator vegetation species and two soil macrofauna orders associated with specific elevations. These results suggest that under a climate-change-driven fog lift, the TMCF of the Orinoco River Basin will be displaced. Moreover, this study provides a baseline to monitor such displacement. 相似文献
982.
The genes phaR, phaP, and phaF, encoding putative regulatory proteins, were found in the poly (3-hydroxybutyrate) (PHB) gene cluster of the free nitrogen-fixing bacteria Azotobacter sp. FA8. These genes were flanked by the insertion sequence ISAzsp1, belonging to the IS3 family, and a region highly homologous to insertion sequences of the IS630 family. These are the first site-specific recombination elements to be described in association with genes involved in the metabolism of polyhydroxyalkanoates (PHAs). A possible role for ISs in the assembly of pha genes is presented. 相似文献
983.
Linn Borgen Nilsen Manfred Heun Shivcharn S. Dhillion Camargo-Ricalde Sara LucÍa Rendón-Aguilar Beatriz 《Economic botany》2005,59(4):366-376
Traditional Knowledge and Genetic Diversity of Opuntia pilifera (Cactaceae) in the Tehuacán-Cuicatlán Valley, Mexico. Economic Botany 59(4)366-376, 2005. Most studies of the genusOpuntia have focused on economically important species, and therefore more knowledge concerning the genetic diversity among wild and locally managedOpuntia species is needed for an expanded use of cacti in the future. The present study is part of ongoing ethnobotanical work in the Tehuacán-Cuicatlán Valley of Mexico and focuses on six traditionally classified forms ofOpuntia pilifera used as food by the indigenous Popoloca people in San Juan Atzingo. Traditional knowledge of how to distinguish these forms based on fruit flavor, color, size, and number of spines on the fruits and cladodes is preserved in the local community. Genetic fingerprinting with 129 AFLPs did not correlate with this traditional morphological classification of 67 cacti. Yet, these AFLPs distinguished the analyzed 67Opuntia pilifera cacti easily from the out-group comprising 17 wildOpuntia velutina. 相似文献
984.
985.
Beatriz Gutiérrez-Gil Jorge Pérez Lorena álvarez Maria Martínez-Valladares Luis-Fernando de la Fuente Yolanda Bayón Aranzazu Meana Fermin San Primitivo Francisco-Antonio Rojo-Vázquez Juan-José Arranz 《遗传、选种与进化》2009,41(1):46
Background
For ruminants reared on grazing systems, gastrointestinal nematode (GIN) parasite infections represent the class of diseases with the greatest impact on animal health and productivity. Among the many possible strategies for controlling GIN infection, the enhancement of host resistance through the selection of resistant animals has been suggested by many authors. Because of the difficulty of routinely collecting phenotypic indicators of parasite resistance, information derived from molecular markers may be used to improve the efficiency of classical genetic breeding.Methods
A total of 181 microsatellite markers evenly distributed along the 26 sheep autosomes were used in a genome scan analysis performed in a commercial population of Spanish Churra sheep to detect chromosomal regions associated with parasite resistance. Following a daughter design, we analysed 322 ewes distributed in eight half-sib families. The phenotypes studied included two faecal egg counts (LFEC0 and LFEC1), anti-Teladorsagia circumcincta LIV IgA levels (IgA) and serum pepsinogen levels (Peps).Results
The regression analysis revealed one QTL at the 5% genome-wise significance level on chromosome 6 for LFEC1 within the marker interval BM4621-CSN3. This QTL was found to be segregating in three out of the eight families analysed. Four other QTL were identified at the 5% chromosome-wise level on chromosomes 1, 10 and 14. Three of these QTL influenced faecal egg count, and the other one had an effect on IgA levels.Conclusion
This study has successfully identified segregating QTL for parasite resistance traits in a commercial population. For some of the QTL detected, we have identified interesting coincidences with QTL previously reported in sheep, although most of those studies have been focused on young animals. Some of these coincidences might indicate that some common underlying loci affect parasite resistance traits in different sheep breeds. The identification of new QTL may suggest the existence of complex host-parasite relationships that have unique features depending on the host-parasite combination, perhaps due to the different mechanisms underlying resistance in adult sheep (hypersensitivity reactions) and lambs (immunity). The most significant QTL identified on chromosome 6 for LFEC1 may be the target for future fine-mapping research efforts. 相似文献986.
Torrado M Nespereira B Bouzamayor Y Centeno A López E Mikhailov AT 《FEBS letters》2006,580(17):4182-4187
Diastolic heart failure (DHF) was produced in 6-day-old piglets by intravenous administration of Doxorubicin, and ANKRD1 protein and mRNA levels were determined in atrial (A) and ventricular (V) chambers of failing vs control hearts. In controls, ANKRD1 showed a left-right (L-R) asymmetric distribution with protein levels 2-fold higher in the LA as compared to the RA, and 8-fold higher in the LV than the RV. In failing hearts, ANKRD1 levels were augmented about 2-fold in each ventricle but equally reduced in both atria as compared to controls. ANKRD1 downregulation in atria is discussed as a process associated with advanced DHF. 相似文献
987.
Orjalo AV Arnaoutov A Shen Z Boyarchuk Y Zeitlin SG Fontoura B Briggs S Dasso M Forbes DJ 《Molecular biology of the cell》2006,17(9):3806-3818
The Nup107-160 complex is a critical subunit of the nuclear pore. This complex localizes to kinetochores in mitotic mammalian cells, where its function is unknown. To examine Nup107-160 complex recruitment to kinetochores, we stained human cells with antisera to four complex components. Each antibody stained not only kinetochores but also prometaphase spindle poles and proximal spindle fibers, mirroring the dual prometaphase localization of the spindle checkpoint proteins Mad1, Mad2, Bub3, and Cdc20. Indeed, expanded crescents of the Nup107-160 complex encircled unattached kinetochores, similar to the hyperaccumulation observed of dynamic outer kinetochore checkpoint proteins and motors at unattached kinetochores. In mitotic Xenopus egg extracts, the Nup107-160 complex localized throughout reconstituted spindles. When the Nup107-160 complex was depleted from extracts, the spindle checkpoint remained intact, but spindle assembly was rendered strikingly defective. Microtubule nucleation around sperm centrosomes seemed normal, but the microtubules quickly disassembled, leaving largely unattached sperm chromatin. Notably, Ran-GTP caused normal assembly of microtubule asters in depleted extracts, indicating that this defect was upstream of Ran or independent of it. We conclude that the Nup107-160 complex is dynamic in mitosis and that it promotes spindle assembly in a manner that is distinct from its functions at interphase nuclear pores. 相似文献
988.
Carolina Inés Domaica Mercedes Beatriz Fuertes Ignacio Uriarte María Victoria Girart Jessica Sarda?ons Dorina Ileana Comas Daniela Di Giovanni María Isabel Gaillard Liliana Bezrodnik Norberto Walter Zwirner 《PloS one》2012,7(12)
Two populations of human natural killer (NK) cells can be identified in peripheral blood. The majority are CD3−CD56dim cells while the minority exhibits a CD3−CD56bright phenotype. In vitro evidence indicates that CD56bright cells are precursors of CD56dim cells, but in vivo evidence is lacking. Here, we studied NK cells from a patient that suffered from a melanoma and opportunistic fungal infection during childhood. The patient exhibited a stable phenotype characterized by a reduction in the frequency of peripheral blood CD3−CD56dim NK cells, accompanied by an overt increase in the frequency and absolute number of CD3−CD56bright cells. These NK cells exhibited similar expression of perforin, CD57 and CD158, the major activating receptors CD16, NKp46, NKG2D, DNAM-1, and 2B4, as well as the inhibitory receptor CD94/NKG2A, on both CD56bright and CD56dim NK cells as healthy controls. Also, both NK cell subpopulations produced IFN-γ upon stimulation with cytokines, and CD3−CD56dim NK cells degranulated in response to cytokines or K562 cells. However, upon stimulation with cytokines, a substantial fraction of CD56dim cells failed to up-regulate CD57 and CD158, showed a reduction in the percentage of CD16+ cells, and CD56bright cells did not down-regulate CD62L, suggesting that CD56dim cells could not acquire a terminally differentiated phenotype and that CD56bright cells exhibit a maturation defect that might result in a potential altered migration pattern. These observations, support the notion that NK cells of this patient display a maturation/activation defect that precludes the generation of mature NK cells at a normal rate accompanied by CD56dim NK cells that cannot completely acquire a terminally differentiated phenotype. Thus, our results provide evidence that support the concept that in vivo CD56bright NK cells differentiate into CD56dim NK cells, and contribute to further understand human NK cell ontogeny. 相似文献
989.
Natal dispersal in great bustards: the effect of sex, local population size and spatial isolation 总被引:1,自引:0,他引:1
Martín CA Alonso JC Alonso JA Palacín C Magaña M Martín B 《The Journal of animal ecology》2008,77(2):326-334
1. We investigated the causes of natal dispersal in four Spanish areas where 35 breeding groups of the polygynous great bustard Otis tarda were monitored intensively. A total of 392 juveniles were radio-tracked between 1991 and 2006 by ground and via aeroplane to avoid potential biases derived from the non-detection of long-distance dispersers. 2. We explored 10 explanatory variables that were related to individual phenotypic features, habitat and conspecific traits in terms of group size and breeding performance, and spatial distribution of available breeding groups. Probability of group change and natal dispersal distances were investigated separately through multifactorial analyses. 3. Natal dispersal occurred in 47.8% of the birds and median natal dispersal distance of dispersers was 18.1 km (range 4.97-178.42 km). Sex largely determined the dispersal probability, with 75.6% of males being dispersers and 80.0% of females being philopatric, in contrast to the general pattern of female-biased dispersal found in most avian species. 4. Both the frequency of natal dispersal and dispersal distances were affected by the spatial distribution of breeding groups. More isolated groups showed a higher proportion of philopatric individuals, the effect being more evident in males than in females. This implies a reduction in gene flow in fragmented populations, as most genetic exchange is achieved through male dispersal. Additionally, dispersers hatched in more isolated groups tended to exhibit longer dispersal distances, which increases the associated energetic costs and mortality risks. 5. The dispersal decision was influenced by the number of conspecifics in the natal group. The individual probability of natal dispersal was related inversely to the size of the natal group, which supports the balanced dispersal model and the conspecific attraction hypothesis. 6. Overall, our results provide a good example of phenotypic plasticity and reinforce the current view that dispersal is an evolutionary complex trait conditioned by the interaction of individual, social and environmental causes that vary between individuals and populations. 相似文献
990.
Phanindhar Shivapooja Changyong Cao Beatriz Orihuela Vrad Levering Xuanhe Zhao Daniel Rittschof 《Biofouling》2016,32(9):1017-1028
Silicone-oil additives are often used in fouling-release silicone coatings to reduce the adhesion strength of barnacles and other biofouling organisms. This study follows on from a recently reported active approach to detach barnacles, which was based on the surface strain of elastomeric materials, by investigating a new, dual-action approach to barnacle detachment using Ecoflex®-based elastomers incorporated with poly(dimethylsiloxane)-based oil additives. The experimental results support the hypothesis that silicone-oil additives reduce the amount of substratum strain required to detach barnacles. The study also de-coupled the two effects of silicone oils (ie surface-activity and alteration of the bulk modulus) and examined their contributions in reducing barnacle adhesion strength. Further, a finite element model based on fracture mechanics was employed to qualitatively understand the effects of surface strain and substratum modulus on barnacle adhesion strength. The study demonstrates that dynamic substratum deformation of elastomers with silicone-oil additives provides a bifunctional approach towards management of biofouling by barnacles. 相似文献