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991.
Jennifer Potter Mae-Wan Ho Hilary Bolton Anna J. Furth Dallas M. Swallow Beatrice Griffiths 《Biochemical genetics》1985,23(5-6):423-439
Human lactase purified from detergent extracts of the total membrane fraction of postmortem jejunum by means of monoclonal immunoadsorbent chromatography appears to be a dimer of subunits identical in Mr (160K). Trypsin or papain removes a small hydrophobic anchoring peptide from each subunit to give a hydrophilic enzyme which no longer interacts with detergent micelles. Lactase hydrolyzes, besides lactose, cellobiose and the synthetic substrates, 4-methylumbelliferyl--galactoside and -glucoside, as well as phlorizin; but it does not hydrolyze glucocerebroside. Phlorizin hydrolase is associated with lactase under all conditions investigated; coincident staining on immunodiffusion and immunoelectrophoresis, coincident elution on immunoadsorbent chromatography and on gel filtration in a dissociating buffer, and correlated reduction in activity in lactase-nonpersistent individuals. Adult and infant lactases are indistinguishable by titration or immunodiffusion against polyclonal rabbit antibodies. Adult individuals low in lactase activity also show a corresponding reduction in cross-reacting material. These observations suggest that lactase persistence is due to the continued synthesis of the infant enzyme.Financial support was provided by the Nuffield Foundation, the Medical Research Council, and the Open University Research Committee Fund. 相似文献
992.
Beatrice M. Sweeney 《Journal of phycology》1978,14(1):116-120
The ultrastructure of the Noctiluca miliaris Suriray from Southest Asian waters which contains the green flagellate, Pedinomonas noctilucae (Subrahmanyan) Sweeney, is in all major respects similar to that of the European strain. New details of the thecal vesicles, pellicle and underlying microtubules are presented. The possibility that the lipid vesicles are identical with the strongly phase-retarding bodies in the surface cytoplasm, some of which are “microsources” of bioluminescence, is suggested. 相似文献
993.
D R Pfeiffer P C Schmid M C Beatrice H H Schmid 《The Journal of biological chemistry》1979,254(22):11485-11494
Liver mitochondria treated with N-ethylmaleimide can accumulate Ca2+ but cannot retain it. Ca2+ loss following uptake occurs in parallel with a proton uptake and collapse of the membrane potential. Respiration is not activated during Ca2+ release and cannot be stimulated by uncoupler. After Ca2+ release and accompanying phenomena are nearly complete, the mitochondria undergo a large amplitude swelling. Nupercaine inhibits the premature release of Ca2+, proton uptake, decline in membrane potential, inhibition of uncoupler-stimulated respiration, and large amplitude swelling. Ruthenium red also prevents these effects. Neither Sr2+ or Mn2+ will substitute for Ca2+ to induce these effects in N-ethylmaleimide-treated mitochondria. The effects of N-ethylmaleimide plus Ca2+ on mitochondria are not accompanied by a significant alteration in the content or composition of phospholipids but are accompanied by small increases in the mitochondrial content of free fatty acids. Free fatty acids accumulate more rapidly in response to limited Ca2+ loading in the absence of N-ethylmaleimide than they do in its presence. In the absence of N-ethylmaleimide, polyunsaturated fatty acids and saturated plus monounsaturated fatty acids accumulate at nearly equal rates. In the presence of N-ethylmaleimide, polyunsaturated fatty acids accumulate more rapidly than saturated plus monounsaturated fatty acids. Any condition or agent tested which inhibited swelling and the other effects produced by Ca2+ plus N-ethylmaleimide also prevented the more rapid accumulation of polyunsaturated, compared to saturated plus monounsaturated, fatty acids. In the light of a positional analysis of phospholipid acyl moieties, these data suggest that 1-acyllysophospholipids accumulate in swelling mitochondria but not in response to noraml Ca2+ loading or when swelling is blocked by other agents. The free fatty acid accumulation, per se, is not responsible for swelling, but levels of exogenous palmitic acid as low as 1 nmol/mg of protein dramatically alter the dependence of swelling velocity on Ca2+ concentration, producing a shift from a sigmoidal- to a hyperbolic-like relationship. This same alteration is brought about by aging the mitochondrial preparation at 0 degrees C. Either pyruvate or DL-carnitine prevents the effect of exogenous palmitate and restores the Aa2+ swelling dependence of aged N-ethylmaleimide-treated mitochondria to that of fresh N-ethylmaleimide-treated mitochondria. Intramitochondrial acylcoenzyme A or acylcarnitine, or both, therefore, to be the modulator of Ca2+ sensitivity rather than free fatty acid. The findings are discussed in terms of the role of intramitochondrial phospholipase and other phospholipid metabolizing enzymes in the mechanisms of N-ethylmaleimide plus Ca2+ effects on mitochondria. 相似文献
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996.
Standard decoding of the genetic information into polypeptides is performed by one of the most sophisticated cell machineries, the translating ribosome, which, by following the genetic code, ensures the correspondence between the mature mRNA and the protein sequence. However, the expression of a minority of genes requires programmed deviations from the standard decoding rules, globally named recoding. This includes ribosome programmed -/+1 frameshifting, ribosome hopping, and stop codon readthrough. Recoding in Archaea was unequivocally demonstrated only for the translation of the UGA stop codon into the amino acid selenocysteine. However, a new recoding event leading to the 22nd amino acid pyrrolysine and the preliminary reports on a gene regulated by programmed -1 frameshifting have been recently described in Archaea. Therefore, it appears that the study of this phenomenon in Archaea is still at its dawn and that most of the genes whose expression is regulated by recoding are still uncharacterized. 相似文献
997.
The detection of dopamine by differential pulse voltammetry (DPV) and square wave voltammetry (SWV) at the interface between two immiscible electrolyte solutions (ITIES) has been studied. Voltammetry at the liquid/liquid (water/1,2-dichloroethane) interface provides a simple method for overcoming the major problem associated with dopamine detection by voltammetry at solid electrodes: the co-existence of ascorbate at higher concentrations. Selectivity for dopamine was achieved by the use of dibenzo-18-crown-6 as an ionophore for the facilitated transfer voltammetry of protonated dopamine across the ITIES. Under these conditions, ascorbate is not transferred and hence does not interfere in the ion transfer current for dopamine. By use of DPV and SWV, the lowest concentration detectable can be lowered from ca. 0.1 mM (obtained with cyclic voltammetry) to 2 microM. Evaluation of the effect of some other physiologically important species (acetylcholine, sodium, potassium and ammonium ions) on the dopamine transfer voltammetry has been studied, indicating the need for improved ionophore designs in order to achieve practically useful selectivity. 相似文献
998.
Surgucheva I Park BC Yue BY Tomarev S Surguchov A 《Cellular and molecular neurobiology》2005,25(6):1009-1033
Summary Mutations in the gene encoding human myocilin are associated with some cases of juvenile and early-onset glaucoma. Glaucomatous
mutations prevent myocilin from being secreted. The analysis of the defects associated with mutations point to the existence
of factor(s) in addition to mutations that might be implicated in the development of glaucoma. In the present paper, we found
that interaction of myocilin with one of the members of the synuclein family alters its properties, including its ability
to be secreted. Results of immunoprecipitation show that myocilin is a γ-synuclein-interacting protein. Further analysis demonstrated
that both myocilin and γ-synuclein are expressed in human TM cells, immortalized rat ganglion (RGC-5) cells, and HT22 hippocampal
neurons. According to Western blotting, in addition to monomeric form with molecular weight 17 kDa γ-synuclein is present
as higher molecular weight forms (∼35 and 68 KDa), presumably dimer and tetramer. Myocilin and γ-synuclein have partially
overlapping perinuclear localization. Dexamethasone upregulates myocilin expression in RGC-5 cells and HT22 hippocampal neurons.
We found alterations of myocilin properties as a result of its interaction with γ-synuclein. In cultured cells, γ-synuclein
upregulates myocilin expression, inhibits its secretion and prevents the formation of high molecular weight forms of myocilin.
Although both α-synuclein and γ-synuclein are expressed in HTM cells, only γ-synuclein interacts with myocilin and alters
its properties.
We conclude that myocilin and γ-synuclein interact and as a result, myocilin's properties are changed. Since myocilin and
γ-synuclein have partially overlapping intracellular localization in cell types that are implicated in glaucoma development,
their interaction may play an important role in glaucoma. 相似文献
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1000.