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131.
For altricial young, fledging is an abrupt step into an unknown environment. Despite increasing numbers of studies addressing the post‐fledging period, our current knowledge of the causes and consequences of post‐fledging survival remains fragmentary. Here, we review the literature on post‐fledging survival of juvenile altricial birds, addressing the following main questions: Is low post‐fledging survival a bottleneck in the altricial reproductive cycle? What is known of proximate and ultimate causal factors such as trophic relations (food and predation), habitat conditions, or abiotic factors acting in the post‐fledging period? We analyzed weekly survival estimates from 123 data series based on studies of 65 species, covering weeks 1–13 post‐fledging. As a general pattern, survival of fledglings was low during the first week post‐fledging (median rate = 0.83), and improved rapidly with time post‐fledging (week 4 median rate = 0.96). For ground‐nesting species, survival immediately after leaving nests was similar to egg‐to‐fledging survival. For species breeding above‐ground, survival during the first week post‐fledging was substantially lower than during both the nestling period and later post‐fledging stages. Thus, the early post‐fledging period is a bottleneck of markedly elevated mortality for most altricial species. Predation was the main proximate cause of mortality. Various factors such as habitat, annual and seasonal variation in the environment, and the physical condition of fledglings have been found to affect post‐fledging survival. Individual survival depended strongly on physical traits such as mass and wing length, which likely influence the ability of fledglings to escape predation. Trophic relationships at various levels are the main ultimate driver of adaptation of traits relevant to survival during the pre‐ and post‐fledging periods. Spatiotemporal dynamics of food resources determine the physical development of juveniles and, in turn, their performance after fledging. However, predators can cause quick and efficient selection for fledgling traits and adult breeding decisions. Parental strategies related to clutch size and timing of breeding, and the age and developmental stage at which young fledge have substantial effects on post‐fledging survival. The intensity and duration of post‐fledging parental investment also influences fledgling survival. Post‐fledging mortality is therefore not a random and inevitable loss. Traits and strategies related to fledging and the post‐fledging stage create large fitness differentials and, therefore, are integral, yet poorly understood, parts of the altricial reproductive strategy. 相似文献
132.
Effects of aluminium treatment on Norway spruce roots: Aluminium binding forms,element distribution,and release of organic substances 总被引:1,自引:1,他引:0
Heim Alexander Luster Jörg Brunner Ivano Frey Beat Frossard Emmanuel 《Plant and Soil》1999,216(1-2):103-116
In order to investigate if Al resistance in Norway spruce (Picea abies[L.] Karst.) can be attributed to similar exclusion mechanisms as they occur in several crop plants, three-year-old Norway
spruce plants were treated for one week in hydroculture with either 500 μM AlCl3 or CaCl2 solutions at pH 4. Sequential root extraction with 1 M NH4Cl and 0.01 M HCl and EDX microanalysis revealed that Al and Ca in cell walls and on the surface participated in exchange processes. About
half of the Al extracted by the sequential extraction was not exchangeable by 1 M NH4Cl. Phenolics and phosphate present in the root extracts are possible ligands for Al adsorbed to or precipitated at the root
in a non-exchangeable form. In both treatments, C release during the first period of 2 d was much higher than during the remaining
time of the experiment. Al treated plants released less total C, carbohydrates and phenolics than did Ca treated plants. Acetate
was the only organic acid anion that could be detected in some samples of both treatments. Free amino acids were present at
micromolar concentrations but as hydrolysis did not increase their yield, there was no evidence of peptide release. One to
two thirds of the released C were large enough not to pass a 1 kDa ultrafilter. The results suggest that exudation of soluble
organic complexors is not a major Al tolerance mechanism in Norway spruce, although complexation of Al by phenolic substances
released by the root could be detected by fluorescence spectroscopy. Aluminium tolerance could rather be attributed to immobilization
in the root apoplast, where strong binding sites are available or precipitation may occur.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
133.
Elias T. Ayuk Bahiru Duguma Steve Franzel Joseph Kengue Matthias Mollet Theophile Tiki-Manga Pauline Zekeng 《Economic botany》1999,53(3):292-301
Dacryodes edulis is one of the most preferred tree species by farmers in the humid lowlands of Cameroon. The fruit of the species figures prominently in cross-boundary trade between Cameroon, Nigeria and Gabon. Although there exist empirical data on the volume of trade of the fruit at this level, no data are available at the farm level. A field survey was undertaken to identify uses, management, and farmers’ improvement objectives and to quantify, at the farm-level, the economic potential of the species. The results of the survey indicate thatD. edulis is widely planted and found mainly in tree crop fields and in home gardens. The fruit is highly consumed and traded. The farm-level value of fruit production reaches $US161 a year per grower or collector. The dead branches of the species are used as firewood and its bark is used as medicine. Desired improvement objectives include increased fruit size, good tasting fruit, high yield and reduced time to bearing. 相似文献
134.
Thomas Wiegand Wei-Chih Liao Ta Chung Ong Alexander Däpp Riccardo Cadalbert Christophe Copéret Anja Böckmann Beat H. Meier 《Journal of biomolecular NMR》2017,69(3):157-164
DNP (dynamic nuclear polarization)-enhanced solid-state NMR is employed to directly detect protein–DNA and protein–ATP interactions and identify the residue type establishing the intermolecular contacts. While conventional solid-state NMR can detect protein–DNA interactions in large oligomeric protein assemblies in favorable cases, it typically suffers from low signal-to-noise ratios. We show here, for the oligomeric DnaB helicase from Helicobacter pylori complexed with ADP and single-stranded DNA, that this limitation can be overcome by using DNP-enhanced spectroscopy. Interactions are established by DNP-enhanced 31P–13C polarization-transfer experiments followed by the recording of a 2D 13C–13C correlation experiment. The NMR spectra were obtained in less than 2 days and allowed the identification of residues of the motor protein involved in nucleotide binding. 相似文献
135.
Albert A. Smith Francesco Ravotti Emilie Testori Riccardo Cadalbert Matthias Ernst Anja Böckmann Beat H. Meier 《Journal of biomolecular NMR》2017,67(2):109-119
Fast magic-angle spinning and partial sample deuteration allows direct detection of 1H in solid-state NMR, yielding significant gains in mass sensitivity. In order to further analyze the spectra, 1H detection requires assignment of the 1H resonances. In this work, resonance assignments of backbone HN and Hα are presented for HET-s(218–289) fibrils, based on the existing assignment of Cα, Cβ, C’, and N resonances. The samples used are partially deuterated for higher spectral resolution, and the shifts in resonance frequencies of Cα and Cβ due to the deuterium isotope effect are investigated. It is shown that the deuterium isotope effect can be estimated and used for assigning resonances of deuterated samples in solid-state NMR, based on known resonances of the protonated protein. 相似文献
136.
Naeela Qureshi Harbans Bariana Kerrie Forrest Matthew Hayden Beat Keller Thomas Wicker Justin Faris Elena Salina Urmil Bansal 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2017,130(3):495-504
Key message
Fine mapping of Yr47 and Lr52 in chromosome arm 5BS of wheat identified close linkage of the marker sun180 to both genes and its robustness for marker-assisted selection was demonstrated.Abstract
The widely effective and genetically linked rust resistance genes Yr47 and Lr52 have previously been mapped in the short arm of chromosome 5B in two F3 populations (Aus28183/Aus27229 and Aus28187/Aus27229). The Aus28183/Aus27229 F3 population was advanced to generate an F6 recombinant inbred line (RIL) population to identify markers closely linked with Yr47 and Lr52. Diverse genomic resources including flow-sorted chromosome survey sequence contigs representing the orthologous region in Brachypodium distachyon, the physical map of chromosome arm 5BS, expressed sequence tags (ESTs) located in the 5BS6-0.81-1.00 deletion bin and resistance gene analog contigs of chromosome arm 5BS were used to develop markers to saturate the target region. Selective genotyping was also performed using the iSelect 90 K Infinium wheat SNP assay. A set of SSR, STS, gene-based and SNP markers were developed and genotyped on the Aus28183/Aus27229 RIL population. Yr47 and Lr52 are genetically distinct genes that mapped 0.4 cM apart in the RIL population. The SSR marker sun180 co-segregated with Lr52 and mapped 0.4 cM distal to Yr47. In a high resolution mapping population of 600 F2 genotypes Yr47 and Lr52 mapped 0.2 cM apart and marker sun180 was placed 0.4 cM distal to Lr52. The amplification of a different sun180 amplicon (195 bp) than that linked with Yr47 and Lr52 (200 bp) in 204 diverse wheat genotypes demonstrated its robustness for marker-assisted selection of these genes.137.
Reif R Sales S Dreier B Lüscher D Wölfel J Gisler C Baici A Kunz B Sonderegger P 《Protein expression and purification》2008,61(1):13-21
An increasing number of studies indicate that serine proteases play an important role in structural plasticity associated with learning and memory formation. Neurotrypsin is a multidomain serine protease located at the presynaptic terminal of neurons. It is thought to be crucial for cognitive brain functions. A deletion in the neurotrypsin gene causes severe mental retardation in humans. For a biochemical characterization, we produced murine neurotrypsin recombinantly in a eukaryotic expression system using myeloma cells. From the culture medium we purified neurotrypsin using heparin-, hydrophobic interaction- and immobilized metal affinity chromatography. For an enzymological characterization two fragments of agrin containing the natural cleavages sites of neurotrypsin were used as substrates. The highest catalytic activity of neurotrypsin was observed in the pH range between 7.0 and 8.5. Calcium ions were required for neurotrypsin activity and an ionic strength exceeding 500 mM decreased substrate cleavage. Site-specific mutations of the amino acids flanking the scissile bonds showed that cleavage is highly specific and requires a basic amino acid preceded by a glutamate residue on the N-terminal side of the scissile bond. This sequence requirement argues for a unique substrate binding pocket of neurotrypsin. This observation was further substantiated by the fact that almost all tested serine protease inhibitors except dichloroisocoumarin and PMSF did not affect neurotrypsin activity. 相似文献
138.
139.
To date, only a few studies using scan-sampling intervals to record neighbours in groups of animals explain how the interval length was chosen. In this study, we investigated the effects of different scan-sampling intervals, behavioural context, and the definition of neighbours on the accuracy of data as compared to a quasi-continuous observation. The data was collected from dairy cows kept in cubicle housing systems. Dairy herds of 22–43 cows on 6 farms were observed once a minute with an automatic tracking system for 6 days, and both the nearest neighbour and all neighbours within a defined distance of each cow were recorded with references to the activity, feeding and lying area and stored in neighbourship matrices. In the analysis, we used data collected at intervals of 2, 3, 4, …, 30 min, and correlated this simulated data with the data based on the 1-min interval using a specialised correlation coefficient for matrices τKr. This correlation coefficient was then used as the response variable in mixed-effects models. We found that the size of the correlation coefficients generally decreased as interval length increased. This decrease was less pronounced for all neighbours than for the nearest neighbour. Moreover, the decrease was greatest with data from the activity area and lowest with data from the lying area. We concluded that, even with the relatively slow dairy cows in a barn environment, neighbour recordings should be conducted at short scan-sampling intervals in order to achieve a minimum correlation of τKr = 0.8 with the quasi-continuous data. Intervals of every 2, 8 and 17 min are recommended for observation of neighbours in dairy cows for the activity, feeding and lying areas, respectively, and species that move faster may well require even shorter sampling intervals. 相似文献
140.
The integration of heifers into free stall dairy herds is a frequent management procedure, but little systematic research has been conducted on its effect on cow behavior. Previous studies mainly focused on aggressive interactions, but it is also of interest how integration affects the spatial distribution of both the cows in the herd and the integrated heifers. In the present study we integrated a single and a pair of heifers on each of six Swiss working farms in a balanced order. Using an automatic tracking system, we recorded the positions of all the cows and of the integrated heifers at 1 min intervals for six continuous 24 h periods. From these data we calculated the proportion of time the animals spent in the activity area, at the feed rack and in the lying cubicles, their average path length and the area of the barn that they used. We then compared the behavior of the integrated heifers with that of the cows in the introductory weeks. We also compared the behavior of the cows recorded in the control weeks directly preceding the integration and in the introductory weeks. For evaluation we used linear mixed-effects models. Singly integrated heifers spent a higher proportion of time in the activity area (0.29 vs. 0.14; P < 0.001) and a lower proportion of time in the lying area (0.40 vs. 0.53; interaction with day, P = 0.011) than the cows, whereas the heifers of the pairs mainly spent a lower proportion of time in the feeding area than the cows (0.23 vs. 0.32; interaction with day, P = 0.044). Average path length was longer for the integrated heifers soon after introduction but approached the values of the cows later on (interaction with day, P = 0.012). The total barn area used by a given animal was largest in the cows and was reduced in heifers integrated singly or in pairs (cows: 341/373 m2, pairs: 306 m2, single heifer: 333 m2; P = 0.055). Cows were little influenced in their space use by the integration of a single or pair of heifers. In summary, the behavior of the singly integrated heifers differed more markedly from that of the cows than the behavior of the heifers introduced in pairs during the introductory week. We would therefore recommend integrating pairs rather than single heifers into herds of dehorned dairy cows to ease their integration. 相似文献