The biosynthesis of kaurenolide diterpenoids by gibberella fujikuroi

The biosynthesis of 7β-hydroxy- and 7β,18-dihydroxy-kaurenolides from ent-kaur-16-en-19-oic acid has been investigated by incubating unlabelled     

Genetic studies on mitochondrially inherited mikamycin-resistance in Paramecium aurelia

Summary Mutants of Paramecium aurelia resistant to mikamycin were shown by microinjection experiments to be due to changes in the extranuclear, mitochondrial genetic system. These mutants were also resistant to a low concentration of erythromycin.Double mutants, resistant to a high level of erythromycin and to mikamycin, were obtained by a two-stage mutation-like process. Injection of mitochondria from these resulted in transformation to the double mutant type, irrespective of the selective medium used to obtain the transformants.     

Preparation and characterization of monoclonal antibodies which recognise different gibberellin epitopes

The production and characterization of high-affinity monoclonal antibodies (McAb) to gibberellins (GAs) is reported. Hybrid myelomas were derived from immunisations with conjugates in which immunogenic proteins were linked to GA₁ at carbon-3 and to GA₄ and GA₉ at carbon-17. A series of McAb which display specificities allowing recognition of, and the discrimination between GA₁, GA₂₀, GA₄ and GA₉ is described. These McAb can be used in quantitative immunoassays for underivatised GAs.Abbreviations BSA bovine serum albumin - FCS foetal calf serum - GA_n gibberellin A_n - IFA incomplete Freund's adjuvant - KLH keyhole-limpet haemocyanin - McAb monoclonal antibody (bodies) - PBS phosphate-buffered saline