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31.
Kuşçu Gökçe Ceren Gürel Çevik Buhur Aylin Oltulu Fatih Akman Levent Köse Timur Yavaşoğlu Nefise Ülkü Karabay Yavaşoğlu Altuğ 《Molecular biology reports》2022,49(3):1721-1729
Molecular Biology Reports - Polycystic ovary syndrome (PCOS) is a metabolic disease that causes infertility due to anovulation in women in reproductive age. It is known that clomiphene citrate (CC)... 相似文献
32.
Changes in phytohormone contents in chickpea seeds germinating under lead or zinc stress 总被引:5,自引:0,他引:5
The present work describes the changes that take place in phytohormone contents in germinating chickpea (Cicer arietinum cv. Aziziye-94) seeds in response to heavy metal stress. For this aim, endogenous abscisic acid (ABA), gibberellic acid (GA3), zeatin (Z) and zeatin riboside (ZR) contents were followed for 24, 48 and 72 h in chickpea seeds germinating at the concentrations of 0.1, 1.0 and 5.0 mM Pb or 0.1, 1.0 and 10 mM Zn. The results showed that Pb and Zn significantly delayed and impeded the germination of chickpea seeds. The negative effect of Pb on germination was higher than that of Zn. Further, Pb increased ABA and Z contents while decreased GA3 content in the germinating seeds. The high concentrations of Zn (1.0 and 10 mM) decreased contents of Z, ZR and GA3 while 0.1 mM Zn increased the content of the same hormones. The ABA content was enhanced by Zn in all concentrations used. 相似文献
33.
The title compound, 2-{4-[3-(2,5-dimethylphenyl)-3-methylcyclobutyl]thiazol-2-yl}isoindoline-1,3-dione (C24H22N2O2S), was synthesized and characterized by IR-NMR spectroscopy and single-crystal X-ray diffraction. The compound crystallizes in the monoclinic space group P21/c with a?=?19.7799(13) Å, b?=?6.7473(4) Å, c?=?15.7259(9) Å and β?=?103.416(5)°. In addition, the molecular geometry, vibrational frequencies and gauge including atomic orbital (GIAO) 1H and 13C chemical shift values of the title compound in the ground state have been calculated by using the Hartree-Fock (HF) and density functional method (DFT/B3LYP) with 6–31G(d), 6–31 + G(d,p) and LANL2DZ basis sets, and compared with the experimental data. To determine conformational flexibility, molecular energy profile of the title compound was obtained by semi-empirical (AM1) calculations with respect to two selected degrees of torsional freedom, which were varied from ?180° to +180° in steps of 5°. Besides, molecular electrostatic potential, frontier molecular orbitals (FMO) analysis and thermodynamic properties of the title compound were investigated by theoretical calculations. 相似文献
34.
Erdogan M Ustüner I Cengiz B Söylemez F Cavdar AO 《Biological trace element research》2006,109(2):105-113
In this article, we report the results of a case control study carried out on 290 Turkish pregnant women at 5–24 wk of gestation
to determine their zinc, folic acid, and vitamin B12 levels in relation to their nutritional and socioconomic status. The women were divided into two groups (n=145 each), depending on the stage of gestation. Group I consisted of women in the first trimester of gestation; those in
the second trimester were allocated into group II. Twenty-five age-matched, healthy nonpregnant women were selected as controls.
The nutritional status of the subjects was determined by means of a survey. Based on this, the women were subdivided into
three subgroups: malnourished (PN), moderately nourished (MN), and well nourished (WN). Also from the survey, the socioeconomic
status was classified as good (G) or bad (B). A statistically significant decrease on zinc and folic acid was observed in
group I women, as their socioeconomic status worsened. In group II, zinc decreased only in the PN and MN subgroups, p<0.001. The folic acid and vitamin B12 levels did not change significantly during the second trimester of gestation in all subgroups. Regarding zinc, nutrition,
or dietary habits are more relevant than socioeconomic status, but poor nutrition affects folic acid levels only during the
first trimester of gestation. 相似文献
35.
Aslı Kurnaz Muazzez Gezelge Aybaba Hançerlioğulları M. Atıf Çetiner Şeref Turhan 《人类与生态风险评估》2016,22(6):1375-1385
The activity concentrations of radionuclides in grape molasses soil samples collected from Zile (Tokat) plain in the Central Black Sea region of Turkey were measured by using gamma spectrometer with a NaI(Tl) detector. Also, the concentrations of 222Rn in soil samples and air were estimated essentially taking the activity concentrations of 226Ra measured in soil samples. Grape molasses soil samples with calcium carbonate content are used for sedimentation for making molasses in this region. The average activity concentrations of 232Th, 226Ra, 40K, and 137Cs were found as 62 ± 2, 68 ± 3, 479 ± 35, and 8.0 ± 0.3 Bq kg?1, respectively. The average concentrations of 222Rn in soil samples and air were estimated to be 50 kBq m?3 and 144 Bq m?3. From the activity concentrations, absorbed gamma dose rate in outdoor air (D), annual effective dose from external exposure (EE), annual effective dose from inhalation of radon (EI), and excess lifetime cancer risk (ELCR) were estimated in order to assess radiological risks. The average values of D, EE, EI, and ELCR were found to be 90 nGy h?1, 110 μSv y?1, 1360 μSv y?1, and 4 × 10?4, respectively. 相似文献
36.
Tunahan Çakır Margriet M. W. B. Hendriks Johan A. Westerhuis Age K. Smilde 《Metabolomics : Official journal of the Metabolomic Society》2009,5(3):318-329
Reverse engineering of high-throughput omics data to infer underlying biological networks is one of the challenges in systems
biology. However, applications in the field of metabolomics are rather limited. We have focused on a systematic analysis of
metabolic network inference from in silico metabolome data based on statistical similarity measures. Three different data
types based on biological/environmental variability around steady state were analyzed to compare the relative information
content of the data types for inferring the network. Comparing the inference power of different similarity scores indicated
the clear superiority of conditioning or pruning based scores as they have the ability to eliminate indirect interactions.
We also show that a mathematical measure based on the Fisher information matrix gives clues on the information quality of
different data types to better represent the underlying metabolic network topology. Results on several datasets of increasing
complexity consistently show that metabolic variations observed at steady state, the simplest experimental analysis, are already
informative to reveal the connectivity of the underlying metabolic network with a low false-positive rate when proper similarity-score
approaches are employed. For experimental situations this implies that a single organism under slightly varying conditions
may already generate more than enough information to rightly infer networks. Detailed examination of the strengths of interactions
of the underlying metabolic networks demonstrates that the edges that cannot be captured by similarity scores mainly belong
to metabolites connected with weak interaction strength. 相似文献
37.
Demet Çetin 《Soil & Sediment Contamination》2009,18(3):345-353
Poly-3-hydroxybutyrate (PHB) film pieces were degraded by sulfate reducing Desulfotomaculum sp. incubated under anaerobic laboratory conditions. Degradation started with adherence of the microbial cells and followed by formation of black colonies on the film surface. Scanning electron microscopic (SEM) observations revealed the presence of bacteria and formation of small holes on the film. After 60 days of incubation at 30°C, 10 % weight loss in polymer and 13 % sulfate reduction in the medium was observed. According to gel permeation chromatography (GPC) analysis, the molecular weight of the PHB decreased after 30 days and did not decrease further at a more extended incubation period. Loss of weight of PHB does not seem to be correlated with molecular weight decrease. 相似文献
38.
A tentative survey on selected marine fish farms was undertaken to delineate the extent to which infections with Staphylococcus species occur in Turkish aquaculture systems. To determine the presence and the distribution of fish pathogenic staphylococci, representative farm types such as marine cages as well as inland pond farms working with saline-ground water and a large hatchery were selected. Field sampling was performed in a total of 13 commercial fish farms that are located in the most-popular aquaculture sites of Turkey. Samples were taken between October 2013 and August 2014. Almost all size classes of the most commonly marine cultured fish species in Turkey were investigated, including Dicentrarchus labrax, Sparus aurata, Argyrosomus regius, Diplodus puntazzo, Dentex dentex, Onchorhynchus mykiss and Salmo labrax. After a visual check, specimens with various clinical signs were sampled for bacteriological and histopathological investigations. Mixed infections of staphylococci along with Vibrio and Aeromonas representatives were detected in Dicentrarchus labrax, Sparus aurata and Diplodus puntazzo samples obtained from 7 farms. The following pathogens were identified: Staphylococcus. epidermidis, S. aureus, S. capitis subsp. capitis, S. lentus, S. hominis subsp. hominis and S. sciuri subsp. sciuri. Main clinical and histopathological effects of the infections in fishes were revealed. The pathogenicity of some isolates was confirmed with in vivo pathogenicity assay and SDS-PAGE analysis. 相似文献
39.
FRANÇOIS MUNOZ THIERRY PAILLER INGRID KOTTKE CÉDRIC GONNEAU MARC‐ANDRÉ SELOSSE 《Molecular ecology》2012,21(20):5098-5109
Characterizing the architecture of bipartite networks is increasingly used as a framework to study biotic interactions within their ecological context and to assess the extent to which evolutionary constraint shape them. Orchid mycorrhizal symbioses are particularly interesting as they are viewed as more beneficial for plants than for fungi, a situation expected to result in an asymmetry of biological constraint. This study addressed the architecture and phylogenetic constraint in these associations in tropical context. We identified a bipartite network including 73 orchid species and 95 taxonomic units of mycorrhizal fungi across the natural habitats of Reunion Island. Unlike some recent evidence for nestedness in mycorrhizal symbioses, we found a highly modular architecture that largely reflected an ecological barrier between epiphytic and terrestrial subnetworks. By testing for phylogenetic signal, the overall signal was stronger for both partners in the epiphytic subnetwork. Moreover, in the subnetwork of epiphytic angraecoid orchids, the signal in orchid phylogeny was stronger than the signal in fungal phylogeny. Epiphytic associations are therefore more conservative and may co‐evolve more than terrestrial ones. We suggest that such tighter phylogenetic specialization may have been driven by stressful life conditions in the epiphytic niches. In addition to paralleling recent insights into mycorrhizal networks, this study furthermore provides support for epiphytism as a major factor affecting ecological assemblage and evolutionary constraint in tropical mycorrhizal symbioses. 相似文献
40.
Daniela Bulgari Adem I. Bozkurt Paola Casati Kadriye Çağlayan Fabio Quaglino Piero A. Bianco 《Antonie van Leeuwenhoek》2012,102(4):677-687
‘Candidatus Phytoplasma mali’, the causal agent of apple proliferation (AP) disease, is a quarantine pathogen controlled by chemical treatments against insect vectors and eradication of diseased plants. In accordance with the European Community guidelines, novel strategies should be developed for sustainable management of plant diseases by using resistance inducers (e.g. endophytes). A basic point for the success of this approach is the study of endophytic bacteria associated with plants. In the present work, endophytic bacteria living in healthy and ‘Ca. Phytoplasma mali’-infected apple trees were described by cultivation-dependent and independent methods. 16S rDNA sequence analysis showed the presence of the groups Proteobacteria, Acidobacteria, Bacteroidetes, Actinobacteria, Chlamydiae, and Firmicutes. In detail, library analyses underscored 24 and 17 operational taxonomic units (OTUs) in healthy and infected roots, respectively, with a dominance of Betaproteobacteria. Moreover, differences in OTUs number and in CFU/g suggested that phytoplasmas could modify the composition of endophytic bacterial communities associated with infected plants. Intriguingly, the combination of culturing methods and cloning analysis allowed the identification of endophytic bacteria (e.g. Bacillus, Pseudomonas, and Burkholderia) that have been reported as biocontrol agents. Future research will investigate the capability of these bacteria to control ‘Ca. Phytoplasma mali’ in order to develop sustainable approaches for managing AP. 相似文献