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81.
Regeneration of many late-successional tree species depends on specialized safe sites. The primary objective was to investigate the roles of seedbed and moisture retention as dimensions of safe sites for the early regeneration of drought-sensitive northern white cedar (Thuja occidentalis). We hypothesized that rates of germination, survival, and growth of T. occidentalis are unlikely to differ among seedbed types under conditions of abundant water, but that differences are likely to emerge as water becomes more limited. In a 67-d greenhouse experiment, cedar seeds were sown on logs, leaf litter, and soil of cedar and paper birch (Betula papyrifera) canopy origin. Seedbeds were subjected to three water treatments. Among the water treatments, highest germination rates occurred within the high water treatment, although germination on cedar litter was comparable to that of the low water treatment. Higher germination and survival rates occurred on decayed logs than other natural seedbeds for medium (P = 0.001) and low (P < 0.0001) water treatments. Germination on birch logs occurred at higher rates than on cedar logs within the low water treatment (P = 0.04). Seedling growth for the medium water treatment was lower on leaf litter than any other type of seedbed (P < 0.01). Results generally demonstrated that the interplay between seedbed and moisture retention is a component of safe sites for T. occidentalis regeneration. 相似文献
82.
Dating the origin of the CCR5-Delta32 AIDS-resistance allele by the coalescence of haplotypes. 总被引:14,自引:2,他引:12
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J C Stephens D E Reich D B Goldstein H D Shin M W Smith M Carrington C Winkler G A Huttley R Allikmets L Schriml B Gerrard M Malasky M D Ramos S Morlot M Tzetis C Oddoux F S di Giovine G Nasioulas D Chandler M Aseev M Hanson L Kalaydjieva D Glavac P Gasparini E Kanavakis M Claustres M Kambouris H Ostrer G Duff V Baranov H Sibul A Metspalu D Goldman N Martin D Duffy J Schmidtke X Estivill S J O'Brien M Dean 《American journal of human genetics》1998,62(6):1507-1515
The CCR5-Delta32 deletion obliterates the CCR5 chemokine and the human immunodeficiency virus (HIV)-1 coreceptor on lymphoid cells, leading to strong resistance against HIV-1 infection and AIDS. A genotype survey of 4,166 individuals revealed a cline of CCR5-Delta32 allele frequencies of 0%-14% across Eurasia, whereas the variant is absent among native African, American Indian, and East Asian ethnic groups. Haplotype analysis of 192 Caucasian chromosomes revealed strong linkage disequilibrium between CCR5 and two microsatellite loci. By use of coalescence theory to interpret modern haplotype genealogy, we estimate the origin of the CCR5-Delta32-containing ancestral haplotype to be approximately 700 years ago, with an estimated range of 275-1,875 years. The geographic cline of CCR5-Delta32 frequencies and its recent emergence are consistent with a historic strong selective event (e.g. , an epidemic of a pathogen that, like HIV-1, utilizes CCR5), driving its frequency upward in ancestral Caucasian populations. 相似文献
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Nagaphani B. Aetukuri Shintaro Kitajima Edward Jung Leslie E. Thompson Kumar Virwani Maria‐Louisa Reich Miriam Kunze Meike Schneider Wolfgang Schmidbauer Winfried W. Wilcke Donald S. Bethune J. Campbell Scott Robert D. Miller Ho‐Cheol Kim 《Liver Transplantation》2015,5(14)
The use of metallic lithium anodes enables higher energy density and higher specific capacity Li‐based batteries. However, it is essential to suppress lithium dendrite growth during electrodeposition. Li‐ion‐conducting ceramics (LICC) can mechanically suppress dendritic growth but are too fragile and also have low Li‐ion conductivity. Here, a simple, versatile, and scalable procedure for fabricating flexible Li‐ion‐conducting composite membranes composed of a single layer of LICC particles firmly embedded in a polymer matrix with their top and bottom surfaces exposed to allow for ionic transport is described. The membranes are thin (<100 μm) and possess high Li‐ion conductance at thicknesses where LICC disks are mechanically unstable. It is demonstrated that these membranes suppress Li dendrite growth even when the shear modulus of the matrix is lower than that of lithium. It is anticipated that these membranes enable the use of metallic lithium anodes in conventional and solid‐state Li‐ion batteries as well as in future Li? S and Li? O2 batteries. 相似文献
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Mark Lipson Po-Ru Loh Sriram Sankararaman Nick Patterson Bonnie Berger David Reich 《PLoS genetics》2015,11(11)
The human mutation rate is an essential parameter for studying the evolution of our species, interpreting present-day genetic variation, and understanding the incidence of genetic disease. Nevertheless, our current estimates of the rate are uncertain. Most notably, recent approaches based on counting de novo mutations in family pedigrees have yielded significantly smaller values than classical methods based on sequence divergence. Here, we propose a new method that uses the fine-scale human recombination map to calibrate the rate of accumulation of mutations. By comparing local heterozygosity levels in diploid genomes to the genetic distance scale over which these levels change, we are able to estimate a long-term mutation rate averaged over hundreds or thousands of generations. We infer a rate of 1.61 ± 0.13 × 10−8 mutations per base per generation, which falls in between phylogenetic and pedigree-based estimates, and we suggest possible mechanisms to reconcile our estimate with previous studies. Our results support intermediate-age divergences among human populations and between humans and other great apes. 相似文献
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Klaus Schümann Nadia Herbach Christina Kerling Markus Seifert Carine Fillebeen Isabella Prysch Jens Reich Günter Weiss Kostas Pantopoulos 《Journal of trace elements in medicine and biology》2010,24(1):58-66
Hemizygous TNFΔARE/+ mice are a murine model for chronic inflammation. We utilized these animals to study iron-kinetics and corresponding protein expression in an iron-deficient and iron-adequate setting. 59Fe-absorption was determined in ligated duodenal loops in vivo. Whole body distribution of i.v. injected 59Fe was analysed, and the organ specific expression of ferroportin, transferrin receptor-1, hepcidin and duodenal DMT-1 was quantified by real-time PCR and Western blotting.Duodenal 59Fe-lumen-to-body transport was not affected by the genotype. Duodenal 59Fe-retention was increased in TNFΔARE/+ mice, suggesting higher 59Fe-losses with defoliated enterocytes. Iron-deficiency increased duodenal 59Fe-lumen-to-body transport, and higher duodenal 59Fe-tissue retention went along with higher duodenal DMT-1, ferroportin, and liver hepcidin expression. TNFΔARE/+ mice significantly increase their 59Fe-content in inflamed joints and ilea, and correspondingly reduce splenic 59Fe-content. Leukocyte infiltrations in the joints suggest a substantial shift of iron-loaded RES cells to inflamed tissues as the underlying mechanism. This finding was paralleled by increased non-haem iron content in joints and reduced haemoglobin and haematocrit concentrations in TNFΔARE/+ mice.In conclusion, erythropoiesis in inflamed TNFΔARE/+ mice could be iron-limited due to losses with exfoliated iron-loaded enterocytes and/or to increased iron-retention in RES cells that shift from the spleen to inflamed tissues. 相似文献