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Xiaomin Chen Su Pan Huimin Bai Jiaxin Fan Wajjiha Batool Ammarah Shabbir Yijuan Han Huakun Zheng Guodong Lu Lili Lin Wei Tang Zonghua Wang 《Molecular Plant Pathology》2023,24(9):1093-1106
Rice blast caused by Magnaporthe oryzae is one of the most destructive diseases and poses a growing threat to food security worldwide. Like many other filamentous pathogens, rice blast fungus releases multiple types of effector proteins to facilitate fungal infection and modulate host defence responses. However, most of the characterized effectors contain an N-terminal signal peptide. Here, we report the results of the functional characterization of a nonclassically secreted nuclear targeting effector in M. oryzae (MoNte1). MoNte1 has no signal peptide, but can be secreted and translocated into plant nuclei driven by a nuclear targeting peptide. It could also induce hypersensitive cell death when transiently expressed in Nicotiana benthamiana. Deletion of the MoNTE1 gene caused a significant reduction of fungal growth and conidiogenesis, partially impaired appressorium formation and host colonization, and also dramatically attenuated the pathogenicity. Taken together, these findings reveal a novel effector secretion pathway and deepen our understanding of rice–M. oryzae interactions. 相似文献
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Ali Irfan Fozia Batool Syeda Andleeb Zahra Naqvi Amjad Islam Sameh M. Osman Alessio Nocentini 《Journal of enzyme inhibition and medicinal chemistry》2013,28(1):265-279
Abstract Benzothiazole (BTA) belongs to the heterocyclic class of bicyclic compounds. BTA derivatives possesses broad spectrum biological activities such as anticancer, antioxidant, anti-inflammatory, anti-tumour, antiviral, antibacterial, anti-proliferative, anti-diabetic, anti-convulsant, analgesic, anti-tubercular, antimalarial, anti-leishmanial, anti-histaminic and anti-fungal among others. The BTA scaffolds showed a crucial role in the inhibition of the metalloenzyme carbonic anhydrase (CA). In this review an extensive literature survey over the last decade discloses the role of BTA derivatives mainly as anticancer agents. Such compounds are effective against various types of cancer cell lines through a multitude of mechanisms, some of which are poorly studied or understood. The inhibition of tumour associated CAs by BTA derivatives is on the other hand better investigated and such compounds may serve as anticancer leads for the development of agents effective against hypoxic tumours. 相似文献
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Fathi Farshid Zamani Batool Piroozmand Ahmad Mozafarpoor Samaneh Seyedhashemi Effat ArefNezhad Reza Motedayyen Hossein 《Molecular biology reports》2021,48(3):2047-2052
Molecular Biology Reports - Programmed death-1 (PD-1), as an immunoinhibitory receptor encoded by programmed cell death-1 (PDCD1) gene, has a pivotal role in tolerance to self-antigens. Mutations... 相似文献
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Zunaira Sher Muhammad Umair Majid Sameera Hassan Fatima Batool Beenish Aftab Bushra Rashid 《Phyton》2021,90(6):1613-1632
Pakistan is facing the threat of Cotton Leaf Curl Virus (CLCuV) which is transmitted through whitefly to cotton crop. Molecular mechanism of leaf epicuticular wax protects the plants from different pathogens including insect attack and disease transmission. Objective of current study is the isolation and characterization of a wax related gene GaCyPI from Gossypium arboreum under CLCuV infection. A fragment of 475 bp was isolated from the total RNA and 3’ and 5’ RACE-PCR products were arranged by overlapping the extended sequences at both the ends. Deduced protein sequence of GaCyPI showed homology with Cyclophilin cis-trans isomerase gene of Gossypium ramondii and Gossypium barbadanse. Multiple sequence alignment also revealed homology among the coding sequences of same gene. GaCyPI protein comprised of 173 amino acids and ORF finder revealed the 69 bases upstream at 5’ while 350 bp at 3’UTR. InterProScan revealed that it belongs to Cyclophilin-type peptidyl prolyl cis/trans isomerase (PPIase) family. Active sites are visible at specific amino acid positions and 3D structure was stable in Ramachandran plot. Prosa server showed protein residues have average 3D-1D score >= 0.2 and Z-Score was −6.74. Phylogenetic analysis revealed that G. raimondii is the closest species that shares the same sequence. Hence, GaCyPI has strong role in plants’ epicuticular wax and its genetic transformation may protect the cotton from whitefly which transmits CLCuV. 相似文献
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Muhammad Zafar-ul-Hye Misbah Batool Zahra Subhan Danish Mazhar Abbas Abdur Rehim Muhammad Naeem Akbar Ayesha Iftikhar Mehreen Gul Ifat Nazir Maria Abid Muhammad Tahzeeb-ul-Hassan Maria Murtaza 《Phyton》2020,89(2):405-413
Rhizosphere bacteria that colonize plant roots and confer beneficial
effects are referred as plant growth promoting rhizobacteria (PGPR). Among all
PGPR, some rhizobacteria have an ability to produce ACC deaminase enzyme.
This enzyme catalyzes stress ACC into a-ketobutyrate and ammonia instead of
letting it to be converted to ethylene. Ethylene level rises in plants under stress
conditions i.e., drought, salinity, poor soil fertility etc. As poor soil fertility is a
big hurdle to achieve the optimum yield of crops, inoculation of ACC deaminase
PGPR can overcome this problem to some extent. The aim of the current study
was to examine the influence of multi-strain and single-strain inoculation of different ACC deaminase producing PGPR on wheat growth and yield. There were
three PGPR strains, Enterobacter cloacae, Serratia ficaria and Burkholderia phytofirmans which were used as consortia and single-strain inoculations. The results
showed that inoculation of E. cloacae + S. ficaria + B. phytofirmans significantly
increased plant height (63%), spike length (61%), number of spikelets spike-1
(61%), number of grains spike-1 (131%), 1000 grains weight (33%), grains yield
(71%), straw yield (71%) and biological yield (68%) of wheat as compared to
control. A significant improvement in N (37 and 200%), P (46 and 166%) and
K (39 and 61%) of seeds and shoot respectively, validated the efficacious and
more effective role of multi-strain (E. cloacae + S. ficaria + B. phytofirmans)
inoculation over control. It is obviously concluded that multi-strain ACC deaminase producing PGPR inoculation is a better approach as compared to singlestrain inoculation for the improvement in growth and yield of wheat. 相似文献
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Maryam Mukhtar Dr Nadeem Sheikh Dr. Andleeb Batool Dr. Muhammad Babar Khawar Dr. Naz Fatima Dr. Rabia Mehmood Dr. 《Saudi Journal of Biological Sciences》2022,29(2):1227-1233
BackgroundCitrullinated proteins formed by peptidyl arginine deiminases (PADIs) deimination of arginine residues in proteins are of particular interest in arthritis pathogenesis. Polymorphisms on the PADI-4 gene lead to the malfunctioning of PADIs leading to the onset of arthritis.ObjectiveThe present study was conducted to determine the polymorphisms on the PADI-4 gene and their association with rheumatoid arthritis (RA) as well as Osteoarthritis (OA).MethodologyTo achieve the above-mentioned objective a case-control study was conducted. Blood samples were collected from RA, OA, and control subjects. DNA was extracted from each blood sample by modified organic method and was quantified as well as qualified by DNA gel electrophoresis and Nanodrop. Patients were tested for rs874881, rs11203366, rs11203367, rs2240336, rs2240337, rs2240339, rs1748033 and rs2240340 polymorphic sites by amplifying targeted regions through PCR with site-specific primers. Genotyping was performed by Restriction Fragment Length Polymorphism and direct sequencing method. Mutations were identified by analyzing sequences on BioEdit software. Allelic, genetic, and multiple site analysis were performed by SHEsis and PLINK software. Change in the amino acid sequence was identified by MEGA 6.0 software.ResultsPolymorphisms were identified on all targeted polymorphic sites except rs2240337 in both RA and OA individuals. In addition, two novel mutations were also identified in exon 4 identified i-e SCV000804840: c.218T > C and SCV000807675: c.241G > T. All the SNPs except rs11203366 were found to be significantly associated with RA at an allelic level whereas all SNP’s have been significant risk factors in the onset of OA. At genotypic level rs874881, rs11203366, rs2240339, SCV000804840 and SCV000807675 were significantly associated to RA development whereas rs874881, rs11203366, rs11203367, rs2240339, SCV000804840 and SCV000807675 were genetic risk factors in OA onset. Haplotype analysis indicated that GACCACGCC and GACCACGCT were highly significant in disease development. Polymorphisms identified altered the functioning of PADIs by altering their amino acid sequence.ConclusionIn conclusion, it was found that PADI-4 gene polymorphism was not only involved in the onset of RA but was also found to be a significant risk factor in OA onset. 相似文献
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Haroon Urooj Khizar Maria Liaquat Fiza Ali Musrat Akbar Mahnoor Tahir Kinza Batool Syeda Saira Kamal Asif Chaudhary Hassan Javed Munis Muhammad Farooq Hussain 《Journal of Plant Growth Regulation》2022,41(6):2435-2448
Journal of Plant Growth Regulation - Soil salinity is one of the main yield-limiting factors in various crops. Under different environmental stresses, many rhizobacteria have demonstrated... 相似文献
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Andleeb Batool Nusrat Jahan Yisuo Sun Atif Hanif Hong Xue 《Molecular biology reports》2014,41(5):3063-3069
A number of genes are known to be involved in glucose homeostasis. Mutations and polymorphisms in candidate genes may effect insulin production, action or resistance. This study was designed to report the association of genetic polymorphism with the type 2 diabetes (T2D) in Pakistani population. A total of 458 subjects (case n = 288, control n = 170) participated in the study. Nine single nucleotide polymorphisms were investigated in genes IDE (rs6583813 C>T, rs7910977 C>T), POU2F1 (rs3767434 A>T, rs10918682 A>T, rs2146727 A>G), WFS1 (rs734312 A>G), PON1 (rs854560 T>A), IL1α (rs1800587 C>T) and IL1β (rs1143634 C>T). Genotyping was performed by DNA sequencing after nested polymerase chain reaction of targeted regions. Results indicated that rs7910977 in IDE showed significant association with the development of T2D [P = 0.012, OR 1.677 (95 % CI 1.112–2.438)]. The rs10918682 in POU2F1 was associated with T2D [P < 0.001, OR 3.606 (95 % CI 2.165–6.005)]. The rs854560 in PON1was associated with incidences of T2D and increased the risk of cardiovascular complications [P = 0.031, OR 0.663 (95 % CI 0.455–0.965)] in diabetics. The rs734312 from WFS1 gene was associated with diabetes at genotype level (P < 0.01). Haplotype analysis of rs1800587–rs1143634 depicted CC haplotype increased the susceptibility to diabetes (P < 0.05). Haplotype GAA from rs2146727–10918682–rs3767434 was protective against diabetes (P < 0.01) and GGA exhibited the association with T2D (P < 0.01). Haplotype CT from rs6583813–rs7910977 was protective against diabetes (P = 0.02). Our study provided evidence to IDE, PON1, WFS1, POU2F1, IL1α and IL1β associated with T2D in Pakistanis. 相似文献