全文获取类型
收费全文 | 914篇 |
免费 | 76篇 |
出版年
2021年 | 15篇 |
2018年 | 12篇 |
2016年 | 8篇 |
2015年 | 18篇 |
2014年 | 23篇 |
2013年 | 26篇 |
2012年 | 27篇 |
2011年 | 26篇 |
2010年 | 28篇 |
2009年 | 27篇 |
2008年 | 30篇 |
2007年 | 29篇 |
2006年 | 23篇 |
2005年 | 33篇 |
2004年 | 29篇 |
2003年 | 29篇 |
2002年 | 23篇 |
2001年 | 25篇 |
2000年 | 23篇 |
1999年 | 32篇 |
1998年 | 19篇 |
1997年 | 10篇 |
1996年 | 9篇 |
1995年 | 8篇 |
1993年 | 11篇 |
1992年 | 23篇 |
1991年 | 20篇 |
1990年 | 29篇 |
1989年 | 15篇 |
1988年 | 21篇 |
1987年 | 13篇 |
1986年 | 22篇 |
1985年 | 19篇 |
1984年 | 20篇 |
1983年 | 11篇 |
1982年 | 9篇 |
1981年 | 19篇 |
1980年 | 10篇 |
1979年 | 24篇 |
1978年 | 24篇 |
1977年 | 11篇 |
1976年 | 11篇 |
1975年 | 11篇 |
1974年 | 12篇 |
1973年 | 15篇 |
1972年 | 9篇 |
1971年 | 8篇 |
1970年 | 9篇 |
1969年 | 11篇 |
1968年 | 7篇 |
排序方式: 共有990条查询结果,搜索用时 218 毫秒
81.
Moreira D von der Heyden S Bass D López-García P Chao E Cavalier-Smith T 《Molecular phylogenetics and evolution》2007,44(1):255-266
Resolution of the phylogenetic relationships among the major eukaryotic groups is one of the most important problems in evolutionary biology that is still only partially solved. This task was initially addressed using a single marker, the small-subunit ribosomal DNA (SSU rDNA), although in recent years it has been shown that it does not contain enough phylogenetic information to robustly resolve global eukaryotic phylogeny. This has prompted the use of multi-gene analyses, especially in the form of long concatenations of numerous conserved protein sequences. However, this approach is severely limited by the small number of taxa for which such a large number of protein sequences is available today. We have explored the alternative approach of using only two markers but a large taxonomic sampling, by analysing a combination of SSU and large-subunit (LSU) rDNA sequences. This strategy allows also the incorporation of sequences from non-cultivated protists, e.g., Radiozoa (=radiolaria minus Phaeodarea). We provide the first LSU rRNA sequences for Heliozoa, Apusozoa (both Apusomonadida and Ancyromonadida), Cercozoa and Radiozoa. Our Bayesian and maximum likelihood analyses for 91 eukaryotic combined SSU+LSU sequences yielded much stronger support than hitherto for the supergroup Rhizaria (Cercozoa plus Radiozoa plus Foraminifera) and several well-recognised groups and also for other problematic clades, such as the Retaria (Radiozoa plus Foraminifera) and, with more moderate support, the Excavata. Within opisthokonts, the combined tree strongly confirms that the filose amoebae Nuclearia are sisters to Fungi whereas other Choanozoa are sisters to animals. The position of some bikont taxa, notably Heliozoa and Apusozoa, remains unresolved. However, our combined trees suggest a more deeply diverging position for Ancyromonas, and perhaps also Apusomonas, than for other bikonts, suggesting that apusozoan zooflagellates may be central for understanding the early evolution of this huge eukaryotic group. Multiple protein sequences will be needed fully to resolve basal bikont phylogeny. Nonetheless, our results suggest that combined SSU+LSU rDNA phylogenies can help to resolve several ambiguous regions of the eukaryotic tree and identify key taxa for subsequent multi-gene analyses. 相似文献
82.
83.
The ability of cells to adhere to each other and to their surrounding extracellular matrices is essential for a multicellular existence. Adhesion provides physical support for cells, regulates cell positioning and enables microenvironmental sensing. The integrins and the syndecans are two adhesion receptor families that mediate adhesion, but their relative and functional contributions to cell-extracellular matrix interactions remain obscure. Recent advances have highlighted connections between the signalling networks that are controlled by these families of receptors. Here we survey the evidence that synergistic signalling is involved in controlling adhesive function and the regulation of cell behaviour in response to the external environment. 相似文献
84.
David Bass Thomas A Richards Lena Matthai Victoria Marsh Thomas Cavalier-Smith 《BMC evolutionary biology》2007,7(1):162
Background
It is much debated whether microbes are easily dispersed globally or whether they, like many macro-organisms, have historical biogeographies. The ubiquitous dispersal hypothesis states that microbes are so numerous and so easily dispersed worldwide that all should be globally distributed and found wherever growing conditions suit them. This has been broadly upheld for protists (microbial eukaryotes) by most morphological and some molecular analyses. However, morphology and most previously used evolutionary markers evolve too slowly to test this important hypothesis adequately. 相似文献85.
Bett Nolan N. Hinch Scott G. Bass Arthur L. Braun Douglas C. Burnett Nicholas J. Casselman Matthew T. Cooke Steven J. Drenner S. Matthew Gelchu Ahmed Harrower William L. Ledoux Roxx Lotto Andrew G. Middleton Collin T. Minke-Martin Vanessa Patterson David A. Zhang Wenming Zhu David Z. 《Hydrobiologia》2022,849(2):385-405
Hydrobiologia - Many of the world’s rivers are dammed, altering the physiology, behaviour, ecology and survival of fish. Integrative research has the potential to improve our understanding of... 相似文献
86.
Genomewide transcriptional signatures of migratory flight activity in a globally invasive insect pest 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Molecular ecology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
87.
Hofinger BJ Russell JR Bass CG Baldwin T dos Reis M Hedley PE Li Y Macaulay M Waugh R Hammond-Kosack KE Kanyuka K 《Molecular ecology》2011,20(17):3653-3668
In barley, the eukaryotic translation initiation factor 4E (eIF4E) gene situated on chromosome 3H is recognized as an important source of resistance to the bymoviruses Barley yellow mosaic virus and Barley mild mosaic virus. In modern barley cultivars, two recessive eIF4E alleles, rym4 and rym5, confer different isolate-specific resistances. In this study, the sequence of eIF4E was analysed in 1090 barley landraces and noncurrent cultivars originating from 84 countries. An exceptionally high nucleotide diversity was evident in the coding sequence of eIF4E but not in either the adjacent MCT-1 gene or the sequence-related eIF(iso)4E gene situated on chromosome 1H. Surprisingly, all nucleotide polymorphisms detected in the coding sequence of eIF4E resulted in amino acid changes. A total of 47 eIF4E haplotypes were identified, and phylogenetic analysis using maximum likelihood provided evidence of strong positive selection acting on this barley gene. The majority of eIF4E haplotypes were found to be specific to distinct geographic regions. Furthermore, the eI4FE haplotype diversity (uh) was found to be considerably higher in East Asia, whereas SNP genotyping identified a comparatively low degree of genome-wide genetic diversity in 16 of 17 tested accessions (each carrying a different eIF4E haplotype) from this same region. In addition, selection statistic calculations using coalescent simulations showed evidence of non-neutral variation for eIF4E in several geographic regions, including East Asia, the region with a long history of the bymovirus-induced yellow mosaic disease. Together these findings suggest that eIF4E may play a role in barley adaptation to local habitats. 相似文献
88.
MicroRNAs (miRNAs) are small regulatory RNAs that are essential in all studied metazoans. Research has focused on the prediction and identification of novel miRNAs, while little has been done to validate, annotate, and characterize identified miRNAs. Using Illumina sequencing, ~20 million small RNA sequences were obtained from Caenorhabditis elegans. Of the 175 miRNAs listed on the miRBase database, 106 were validated as deriving from a stem-loop precursor with hallmark characteristics of miRNAs. This result suggests that not all sequences identified as miRNAs belong in this category of small RNAs. Our large data set of validated miRNAs facilitated the determination of general sequence and structural characteristics of miRNAs and miRNA precursors. In contrast to previous observations, we did not observe a preference for the 5' nucleotide of the miRNA to be unpaired compared to the 5' nucleotide of the miRNA*, nor a preference for the miRNA to be on either the 5' or 3' arm of the miRNA precursor stem-loop. We observed that steady-state pools of miRNAs have fairly homogeneous termini, especially at their 5' end. Nearly all mature miRNA-miRNA* duplexes had two nucleotide 3' overhangs, and there was a preference for a uracil in the first and ninth position of the mature miRNA. Finally, we observed that specific nucleotides and structural distortions were overrepresented at certain positions adjacent to Drosha and Dicer cleavage sites. Our study offers a comprehensive data set of C. elegans miRNAs and their precursors that significantly decreases the uncertainty associated with the identity of these molecules in existing databases. 相似文献
89.
Cortes-Bratti X Bassères E Herrera-Rodriguez F Botero-Kleiven S Coppotelli G Andersen JB Masucci MG Holmgren A Chaves-Olarte E Frisan T Avila-Cariño J 《PloS one》2011,6(2):e16960
Background
Thioredoxin 80 (Trx80) is an 80 amino acid natural cleavage product of Trx, produced primarily by monocytes. Trx80 induces differentiation of human monocytes into a novel cell type, named Trx80-activated-monocytes (TAMs).Principal Findings
In this investigation we present evidence for a role of TAMs in the control of intracellular bacterial infections. As model pathogens we have chosen Listeria monocytogenes and Brucella abortus which replicate in the cytosol and the endoplasmic reticulum respectively. Our data indicate that TAMs efficiently inhibit intracellular growth of both L. monocytogenes and B. abortus. Further analysis shows that Trx80 activation prevents the escape of GFP-tagged L. monocytogenes into the cytosol, and induces accumulation of the bacteria within the lysosomes. Inhibition of the lysosomal activity by chloroquine treatment resulted in higher replication of bacteria in TAMs compared to that observed in control cells 24 h post-infection, indicating that TAMs kill bacteria by preventing their escape from the endosomal compartments, which progress into a highly degradative phagolysosome.Significance
Our results show that Trx80 potentiates the bactericidal activities of professional phagocytes, and contributes to the first line of defense against intracellular bacteria. 相似文献90.
In this issue of Cell, Prasanth et al. (2005) provide evidence that an inosine-containing RNA that is normally retained in the nucleus is cleaved within its 3' untranslated region following cellular stress. It is then transported to the cytoplasm and translated into protein. These findings suggest that the nucleus may store RNAs destined for translation that then can be released, as needed, in response to specific cellular signals. 相似文献