Identification of a hypothetical membrane protein interactor of ribosomal phosphoprotein P0

The ribosomal phosphoprotein P0 of the human malarial parasitePlasmodium falciparum (PfP0) has been identified as a protective surface protein. InDrosophila, P0 protein functions in the nucleus. The ribosomal function of P0 is mediated at the stalk of the large ribosomal subunit at the GTPase centre, where the elongation factor eEF2 binds. The multiple roles of the P0 protein presumably occur through interactions with other proteins. To identify such interacting protein domains, a yeast two-hybrid screen was carried out. Out of a set of sixty clones isolated, twelve clones that interacted strongly with both PfP0 and theSaccharomyces cerevisiae P0 (ScP0) protein were analysed. These belonged to three broad classes: namely (i) ribosomal proteins; (ii) proteins involved in nucleotide binding; and (iii) hypothetical integral membrane proteins. One of the strongest interactors (clone 67B) mapped to the gene YFL034W which codes for a hypothetical integral membrane protein, and is conserved amongst several eukaryotic organisms. The insert of clone 67B was expressed as a recombinant protein, and immunoprecipitaion (IP) reaction with anti-P0 antibodies pulled down this protein along with PfP0 as well as ScP0 protein. Using deletion constructions, the domain of ScP0, which interacted with clone 67B, was mapped to 60–148 amino acids. It is envisaged that the surface localization of P0 protein may be mediated through interactions with putative YFL034W-like proteins inP. falciparum     

Discrimination of signals simulating movement of a sound source by dogs after ablation of the auditory cortex

The ability to perceive a moving sound image at dichotic stimulation was studied by means of avoidance technique for decorticated (AI, AII, Ep) dogs. The bilateral ablation disturbed the temporal cue discrimination of the direction of movement. But the animals retained the ability to localize the moving signal using delta I-cue.     

Clubbed thiazoles by MAOS: a novel approach to cyclin-dependent kinase 5/p25 inhibitors as a potential treatment for Alzheimer's disease

A novel clubbed triazolyl thiazole series of cdk5/p25 inhibitors, potentially useful for the treatment of Alzheimer's disease, is disclosed. Evaluation of the SAR of substitution within these series has allowed the identification of a range of compounds which significantly reduce brain cdk5/p25 and thus have potential as possible treatments for Alzheimer's disease.     

Assessment of the mutagenic activity of aversectin C]

Aversectin C was evaluated for mutagenic activity in the Ames test with Salmonella typhimurium TA 97, TA 98 and TA 100, in the dominant lethal assay on uninbred albino rats in a dose of 2.25 mg/kg body weight (1/40 of the LD50) and in the metaphase test on F1CBAxC57BI/6 mice in a dose of 8.2 mg/kg body weight (1/5 of the LD50). The agent showed no mutagenic activity in any of the tests. The anaphase test on F1CBAxC57BI/6 mice revealed no antimitotic activity of aversectin C.     

RNA synthesis in rat brain during pharmacological modification of norepinephrine metabolism

Norepinephrine metabolism and nuclear RNA (nRNA) synthesis in the rat brain are found to be conjugated. Under the effect of preparations inducing a disturbance in the norepinephrine (sodium diethyldithiocarbamate and reserpine) its content in the brain tissue lowers and the nRNA synthesis intensity decreases. Accumulation on total resources of norepinephrine in the brain under the effect of ipraside or its synaptic form (melipramine, Lu-5) intensifies the nRNA synthesis.     

Substantiation of hygienic standard of lincomycin in the air of the work area

The main parameters of lincomycin toxicometry were studied in animals. It was shown that the compound was low toxic after its oral or intraperitoneal administration in single doses, had no local irritant and skin resorptive effects and did not accumulate. The allergenic properties were slightly pronounced. The intoxication picture after a single inhalation was characterized by renal dysfunction, erythropenia, neutrophilia, lymphopenia and impairment of the normal intestinal microflora. The zone of the specific antimicrobial effect was equal to 8. On chronic inhalation, the signs of the specific antimicrobial effect were of the paramount importance: Limch am was equal to 4.7 mg/m3 and Limch exceeded 18.3 mg/m3. In the concentrations used, the substance had no embryotoxic and gonadotropic effects. The level of 0.5 mg/m3 (for Hazard Class 2) was recommended and approved as the maximum allowable concentration.     

Experimental data on hygienic norms for antibiotics of the tetracycline group]

Toxicity of tetracyclines was studied experimentally on different species of laboratory animals. It was shown that tetracycline, oxytetracycline and chlortetracycline were close by their chemical structure and physico-chemical properties, as well as by the main toxicity parameters, i.e. acute toxicity, cumulative activity, skin-irritating and sensitizing effect. Under the conditions of subacute experiments the above 3 antibiotics induced evenly pronounced one direction changes in animals. The data obtained during the experiments provided recommendation of the level of 0.1 mg/m3 as the maximum allowable concentration (MAC) of oxytetracycline and chlortetracycline, i.e. the same level as the previously recommended for tetracycline.