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排序方式: 共有93条查询结果,搜索用时 15 毫秒
31.
Dichio Bartolomeo Xiloyannis Cristos Angelopoulos Kostas Nuzzo Vitale Bufo Sabino A Celano Giuseppe 《Plant and Soil》2003,257(2):381-389
The effects of water stress on water potential components, tissue water content, mean elastic modulus and the osmoregulation capacity of olive (Olea europaea L. cv. Coratina) leaves was determined. Artificial rehydration of olive leaf tissues altered the P-V relationships so that a plateau phenomenon occurred. Points in the P-V curve in the region affected by the plateau, generally up to –0.5 MPa, were corrected for all the samples analyzed. In the corrected P-V relationship, an osmotic adjustment was found in drought-stressed leaf tissues. Osmotic potentials at full turgor (0 (sat)) and osmotic potential at turgor-loss (0 (TVT)) decreased from –2.06±0.01 MPa and –3.07±0.16 MPa in controls to –2.81±0.03 MPa and –3.85±0.12 MPa in most stressed plants. Osmotic adjustment values obtained from the P-V curves agreed with those obtained using an osmometer. An active osmotic adjustment of 1.42 MPa was also observed in 1–4 mm- diameter roots. Mannitol is the main carbohydrate involved in osmotic potential decrease in all treatments. The maximum elastic modulus increased from 11.6±0.95 MPa in the controls to 18.6±0.61 MPa in the most stressed plants. 相似文献
32.
Anna Latiano Orazio Palmieri Luca Pastorelli Maurizio Vecchi Theresa T. Pizarro Fabrizio Bossa Giuseppe Merla Bartolomeo Augello Tiziana Latiano Giuseppe Corritore Alessia Settesoldi Maria Rosa Valvano Renata D’Incà Laura Stronati Vito Annese Angelo Andriulli 《PloS one》2013,8(4)
Background
Recent evidence suggests that the IL-33/IL1RL1 axis plays a critical role in several autoimmune and inflammatory disorders; however, its mechanistic role in inflammatory bowel disease (IBD) has not been clearly defined. We investigated the contribution of IL-33 and IL1RL1 polymorphisms to IBD risk, and possible correlations with phenotype in an Italian cohort of adult and pediatric patients.Methods
We evaluated the association of six SNPs in IL-33 and IL1RL1 genes, in 805 Crohn’s disease (CD), 816 ulcerative colitis (UC), and 752 controls, using Taqman. IL-33 and IL1RL1 mRNA expression was also analyzed.Results
Significant allele and genotype associations with IL-33 rs3939286 were found in CD (P = 0.004; P = 0.035) and UC patients (P = 0.002; P = 0.038). After stratifying the cohort for age at diagnosis, the differences remained significant only in the IBD adult-onset. Significant associations were also obtained in CD patients with two IL1RL1 polymorphisms (rs13015714 and rs2058660, P<0.015). By combining homo- and heterozygous carriers of the rs13015714 risk allele, differences were still significant for both CD adult- and pediatric-onset. Upon genotype-phenotype evaluation, an increased frequency of extensive colitis in adult UC (P = 0.019) and in steroid-responsive pediatric patients (P = 0.024) carrying the IL-33 rs3939286 risk genotype, was observed. mRNA expression of IL-33 and IL1RL1 in inflamed IBD biopsy samples was significantly increased.Conclusions
Common IL-33 and IL1RL1 polymorphisms contribute to the risk of IBD in an Italian cohort of adult and pediatric patients, with some influence on sub-phenotypes. 相似文献33.
Pietrantonio F Biondani P de Braud F Pellegrinelli A Bianchini G Perrone F Formisano B Di Bartolomeo M 《Translational oncology》2012,5(3):155-159
OBJECTIVE: Bax protein is a key mediator of apoptosis, and it might be related to chemosensitivity. The purpose of this study was to evaluate the prognostic role of Bax in patients with advanced gastric cancer treated with triplet chemotherapy COI regimen (capecitabine, oxaliplatin, and irinotecan). METHODS: Pretreatment tissue blocks were available for 23 consecutive patients, selected for good performance status (ECOG ≤ 1) and consenting for treatment with first-line COI at a single institution. Bax levels were classified as positive or negative by immunohistochemistry (bax N20; Santa Cruz Biotechnology) and related to outcome in terms of response rate, progression-free survival, and overall survival. RESULTS: Bax-negative and -positive samples were 26% and 74%, respectively. Bax expression was associated with significantly higher response rate (87% vs 33%), progression-free survival (8.7 vs 4.9 months, P = .016), and overall survival (23.8 vs 12.7 months, P = .025). In multivariate analysis including Bax and performance status, low Bax independently predicted worse outcome, along with suboptimal performance status. CONCLUSIONS: In advanced gastric cancer, Bax expression was related to clinical benefit with COI regimen. Whether Bax is a prognostic or mixed prognostic/predictive factor warrants prospective confirmation. It is to be defined if Bax predicts sensitivity to platinum analogs or to whatever chemotherapy regimen. 相似文献
34.
Luigina Cellini Emanuela Di Campli Michele Masulli Soraya Di Bartolomeo Nerino Allocati 《FEMS immunology and medical microbiology》1996,13(4):273-277
Abstract The antibacterial effect of aqueous garlic extract (AGE) was investigated against Helicobacter pylori . Sixteen clinical isolates and three reference strains of H. pylori were studied. Two different varieties of garlic were used. The concentration of AGE required to inhibit the bacterial growth was between 2–5 mg ml−1 . The concentration, for both AGE types, to inhibit 90% (MIC90 ) of isolates was 5 mg ml−1 . The minimum bactericidal concentration (MBC) was usually equal to, or two-fold higher than, minimum inhibitory concentration (MIC). Heat treatment of extracts reduced the inhibitory or bactericidal activity against H. pylori ; the boiled garlic extract showed a loss of efficacy from two-to four-fold the values of MIC and the MBC obtained with fresh AGR. The antibacterial activity of garlic was also studied after combination with a proton pump-inhibitor (omeprazole) in a ratio of 250:1. A synergistic effect was found in 47% of strains studied; an antagonistic effect was not observed. 相似文献
35.
Carmela Fusco Barbara Mandriani Martina Di Rienzo Lucia Micale Natascia Malerba Dario Cocciadiferro Eva Sjøttem Bartolomeo Augello Gabriella Maria Squeo Maria Teresa Pellico Ashish Jain Terje Johansen Gian Maria Fimia Giuseppe Merla 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》2018,1865(6):908-919
Autophagy is a catabolic process needed for maintaining cell viability and homeostasis in response to numerous stress conditions. Emerging evidence indicates that the ubiquitin system has a major role in this process. TRIMs, an E3 ligase protein family, contribute to selective autophagy acting as receptors and regulators of the autophagy proteins recognizing endogenous or exogenous targets through intermediary autophagic tags, such as ubiquitin. Here we report that TRIM50 fosters the initiation phase of starvation-induced autophagy and associates with Beclin1, a central component of autophagy initiation complex. We show that TRIM50, via the RING domain, ubiquitinates Beclin 1 in a K63-dependent manner enhancing its binding with ULK1 and autophagy activity. Finally, we found that the Lys-372 residue of TRIM50, critical for its own acetylation, is necessary for its E3 ligase activity that governs Beclin1 ubiquitination. Our study expands the roles of TRIMs in regulating selective autophagy, revealing an acetylation-ubiquitination dependent control for autophagy modulation. 相似文献
36.
Emanuela Di Campli Soraya Di Bartolomeo Rossella Grande Mara Di Giulio Luigina Cellini 《Current microbiology》2010,60(6):412-418
The aim of this work was to investigate the effects of exposure to extremely low-frequency electromagnetic fields (ELF-EMF)
both on biofilm formation and on mature biofilm of Helicobacter pylori. Bacterial cultures and 2-day-old biofilm of H. pylori ATCC 43629 were exposed to ELF-EMF (50 Hz frequency–1 mT intensity) for 2 days to assess their effect on the cell adhesion
and on the mature biofilm detachment, respectively. All the exposed cultures and the respective sham exposed controls were
studied for: the cell viability status, the cell morphological analysis, the biofilm mass measurement, the genotypic profile,
and the luxS and amiA gene expression. The ELF-EMF acted on the bacterial population during the biofilm formation displaying significant differences
in cell viability, as well as, in morphotypes measured by the prevalence of spiral forms (58.41%) in respect to the controls
(33.14%), whereas, on mature biofilm, no significant differences were found when compared to the controls. The measurement
of biofilm cell mass was significantly reduced in exposed cultures in both examined experimental conditions. No changes in
DNA patterns were recorded, whereas a modulation in amiA gene expression was detected. An exposure to ELF-EMF of H. pylori biofilm induces phenotypic changes on adhering bacteria and decreases the cell adhesion unbalancing the bacterial population
therefore reducing the H. pylori capability to protect itself. 相似文献
37.
The dynamic interaction of AMBRA1 with the dynein motor complex regulates mammalian autophagy 总被引:1,自引:0,他引:1
Di Bartolomeo S Corazzari M Nazio F Oliverio S Lisi G Antonioli M Pagliarini V Matteoni S Fuoco C Giunta L D'Amelio M Nardacci R Romagnoli A Piacentini M Cecconi F Fimia GM 《The Journal of cell biology》2010,191(1):155-168
Autophagy is an evolutionary conserved catabolic process involved in several physiological and pathological processes such as cancer and neurodegeneration. Autophagy initiation signaling requires both the ULK1 kinase and the BECLIN 1-VPS34 core complex to generate autophagosomes, double-membraned vesicles that transfer cellular contents to lysosomes. In this study, we show that the BECLIN 1-VPS34 complex is tethered to the cytoskeleton through an interaction between the BECLIN 1-interacting protein AMBRA1 and dynein light chains 1/2. When autophagy is induced, ULK1 phosphorylates AMBRA1, releasing the autophagy core complex from dynein. Its subsequent relocalization to the endoplasmic reticulum enables autophagosome nucleation. Therefore, AMBRA1 constitutes a direct regulatory link between ULK1 and BECLIN 1-VPS34, which is required for core complex positioning and activity within the cell. Moreover, our results demonstrate that in addition to a function for microtubules in mediating autophagosome transport, there is a strict and regulatory relationship between cytoskeleton dynamics and autophagosome formation. 相似文献
38.
Alfredo Di Filippo Franco Biondi Katarina ufar Martín de Luis Michael Grabner Maurizio Maugeri Emanuele Presutti Saba Bartolomeo Schirone Gianluca Piovesan 《Journal of Biogeography》2007,34(11):1873-1892
Aim To identify the dominant spatial patterns of Fagus sylvatica radial growth in the Eastern Alps, and to understand their relationships to climate variation and bioclimatic gradients. Location Fourteen beech stands in the Eastern Alps, growing between 200 and 1500 m a.s.l. in Italy, Slovenia and Austria. Methods At each site, trees were sampled using increment borers or by taking discs from felled trees. Cores and discs were processed by measuring and crossdating ring width. Ring width series were standardized, averaged, and prewhitened to obtain site chronologies. Hierarchical Cluster Analysis (HCA) and Principal Components Analysis of prewhitened site chronologies were used to identify spatial and altitudinal growth patterns, related to the bioclimatic position of each stand. Bootstrap correlation and response functions were computed between monthly climatic variables and either principal component scores or composite chronologies from stands associated by HCA. The stability of dendroclimatic signals was analyzed by moving correlation functions (MCF). Correlation analysis (teleconnections) based on a data base of 37 Italian and Slovenian beech tree‐ring chronologies revealed the spatial extent of principal component scores. Results Sampled trees were 200–400 years old, representing the oldest beech trees that have been crossdated for the Alps to date. Maximum age was directly related to altitude and to the presence of historical forms of conservation. Tree‐ring parameters varied according to geographic patterns and the age of sampled trees. Stands were bioclimatically organized according to their location, and with reference to their elevation and distance from the Adriatic Sea. A direct response to winter temperature was found at all elevations. The altitudinal gradient ranged from low‐elevation stands, characterized by a Mediterranean‐type, late spring–summer drought signal, to mountain and high‐elevation stands, characterized by a direct response to growing season temperature plus an inverse response to the previous year’s July temperature. The mountain and high‐elevation signal was evident in Austria, the Central Alps and Slovenia, while the low‐elevation signal was confined to mountains adjacent to the Adriatic Sea. MCF revealed trends in the response to climatic factors affecting tree‐ring formation in mountain and high‐mountain stands linked to climatic warming. Main conclusions Dendroclimatic networks can be used for bioclimatic studies of tree populations. A biogeographical separation emerged between the Alps and the Apennines at the upper elevations, while different degrees of mediterraneity distinguished sites at lower elevations. This information will be useful in assessing any future climate‐related bioclimatic shifts, especially for forests at ecotones and along altitudinal gradients. 相似文献
39.
Mariarosaria Mazzeo Bartolomeo Dichio Michael J. Clearwater Giuseppe Montanaro Cristos Xiloyannis 《Annals of botany》2013,112(1):197-205