全文获取类型
收费全文 | 1219篇 |
免费 | 111篇 |
出版年
2021年 | 11篇 |
2020年 | 8篇 |
2019年 | 9篇 |
2018年 | 17篇 |
2017年 | 15篇 |
2016年 | 19篇 |
2015年 | 32篇 |
2014年 | 32篇 |
2013年 | 43篇 |
2012年 | 72篇 |
2011年 | 57篇 |
2010年 | 45篇 |
2009年 | 50篇 |
2008年 | 59篇 |
2007年 | 76篇 |
2006年 | 60篇 |
2005年 | 54篇 |
2004年 | 56篇 |
2003年 | 47篇 |
2002年 | 51篇 |
2001年 | 44篇 |
2000年 | 36篇 |
1999年 | 26篇 |
1998年 | 15篇 |
1997年 | 16篇 |
1996年 | 15篇 |
1995年 | 12篇 |
1994年 | 9篇 |
1993年 | 15篇 |
1992年 | 23篇 |
1991年 | 25篇 |
1990年 | 16篇 |
1989年 | 15篇 |
1988年 | 16篇 |
1987年 | 9篇 |
1986年 | 8篇 |
1985年 | 10篇 |
1984年 | 15篇 |
1979年 | 10篇 |
1978年 | 9篇 |
1977年 | 10篇 |
1976年 | 12篇 |
1975年 | 13篇 |
1974年 | 10篇 |
1973年 | 14篇 |
1972年 | 16篇 |
1971年 | 11篇 |
1970年 | 10篇 |
1968年 | 13篇 |
1967年 | 8篇 |
排序方式: 共有1330条查询结果,搜索用时 15 毫秒
31.
Daniel Barthélémy 《Acta biotheoretica》1991,39(3-4):309-323
Each plant can be recognized by its general shape. Nevertheless, this physiognomy is the result of a very precise structure that expresses the existence of a strong organization. The architecture of a plant depends on the nature and relative arrangement of each of its parts; it is at any given time the result of an equilibrium between endogenous growth processes and the constraints exerted by the environment. Architectural studies have been carried out for some twenty years and have led to the definition of several concepts that provide a powerful tool for studying plant form. The results obtained in this field show that the architecture of a plant can be summarized by a small number of elementary structures: internode, growth unit, axis, architectural model,... In the course of ontogenesis, these structures are repeated and reveal several levels of organization that seem to be only different stages of a common process of growth and transformation.
Résumé Toute espèce végétale peut être reconnue par sa forme générale. Toutefois, au-delà de cet aspect physionomique, la forme d'une plante repose sur une structure très précise qui traduit l'existence d'une grande organisation. L'architecture d'une plante dépend de la nature et de la position relative de ses différentes parties; elle est à tout instant, l'expression d'un èquilibre entre des processus endogénes de croissance et des contraintes extérieures exercées par le milieu. D'origine assez récente, les études en architecture végétale ont permis de dégager quelques concepts qui rendent compte du développement des végétaux. Les résultats obtenus dans ce domaine montrent que l'architecture d'une plante peut être résumée par un petit nombre de structures élémentaires: entre-noeud, unité de croissance, axe, modèle architectural,... Au cours du développement, ces structures élémentaires se répètent et dérivent les unes des autres traduisant l'existence de plusieurs niveaux d'organisation au sein de l'organisme végétal. L'analyse architecturale permet de révéler et de caractériser ces différents niveaux qui apparaissent alors comme les étapes d'un même processus dans une séquence précise et ordonnée d'événements.相似文献
32.
33.
Conjugal transfer system of plasmid RP4: analysis by transposon 7 insertion. 总被引:25,自引:14,他引:11
下载免费PDF全文
![点击此处可从《Journal of bacteriology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
We have begun an analysis in Escherichia coli of the conjugal transfer functions of the broad-host-range plasmid RP4. We have isolated 19 tra mutants of RP4, generated by insertion of transposon 7, and mapped their insertion sites by restriction endonuclease analysis. These sites fall into two separate regions on either side of the kanamycin resistance determinant. The transfer rates of the mutants range from 10% of that of RP4 to an undetectable level. Spot tests with the P-1 pilus-specific phages PRR1, Pf3, and PR4 and electron microscopic examination for pili have classified the mutants into several phenotypes consistent with their having normal, retracted, or no pili. Analysis of transient plasmid heterozygotes, created by P1 transduction, divided the tra mutants into a minimum of five complementation groups. Some of these groups contain more than one phenotypic class and may represent more than one gene because of the possible polar and deletion effects of Tn7 insertion. 相似文献
34.
Plasmid-determined beta-lactamase indistinguishable from the chromosomal beta-lactamase of Escherichia coli. 总被引:2,自引:2,他引:0
下载免费PDF全文
![点击此处可从《Journal of bacteriology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
M M Bobrowski M Matthew P T Barth N Datta N J Grinter A E Jacob P Kontomichalou J W Dale J T Smith 《Journal of bacteriology》1976,125(1):149-157
A plasmid, derived from a naturally occurring strain of Proteus mirabilis, conferred resistance to cephalosporins, apparently mediated by a beta-lactamase indistinguishable from that determined by the chromosomal gene of Escherichia coli K-12. There was evidence for a recombination event between the wild-type plasmid and a defective F factor (Fsp) in the Escherichia coli K-12 culture in which it was stored. 相似文献
35.
Paul Sroka Robert H. Barth Lawrence I. Gilbert Gerardus B. Staal 《Journal of insect physiology》1975,21(2):463-469
Exogenously applied natural juvenile hormones (JH) and some synthetic JH mimetic substances resulted in dose-related gonadotropic responses and were able to fully substitute for the absence of endogenous JH in a moth and a cockroach. Aliphatic methyl and ethyl esters, thio esters, and amides were most active. Some aromatic JH mimics with high activity in morphogenetic assays on a variety of insects showed little activity in the gonadotropic bioassay. This suggests that the ovaries of the moth Manduca sexta and the cockroach Nauphoeta cinerea respond primarily to the intrinsic JH activity of the test substances. 相似文献
36.
T. Friedel F. G. Barth 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1997,180(3):223-233
Spiders can use air particle movements to localize moving prey. We studied the responses of 32 wind-sensitive interneurones
in the hunting spider Cupiennius salei to prey stimuli.
Stimulation with a tethered flying fly or with artificial air pulses activated plurisegmental interneurones that responded
to changes in air movement velocity and were thus well suited to represent the highly fluctuating air stream typical of prey
stimuli. In most interneurones (n = 18) the responses to the stimulation of different legs were not significantly different from each other.
Different interneurones had different response characteristics and their latencies largely overlapped suggesting that there
is parallel processing of the signals by populations of interneurones with different response characteristics.
In two interneurones the number of spikes and the spiking pattern elicited by stimulation of each of the eight legs markedly
differed depending on the leg stimulated. These neurones may play an important role in directional information processing.
Stimulation of the adjacent legs from front to back or from back to front revealed two interneurones sensitive to the direction
of successive stimulation of the legs. These neurones may be able to detect the motion of an air movement source in a preferred
direction and thus act as nearfield motion detectors to localize a moving prey item.
Accepted: 28 September 1996 相似文献
37.
38.
39.
Mélanie Aye Carole Di Giorgio Isabelle Berque-Bestel Ahissan Aime Benoit P. Pichon Yves Jammes Philippe Barthélémy Michel De Méo 《Mutation Research - Genetic Toxicology and Environmental Mutagenesis》2013,750(1-2):129-138
We proposed to evaluate the genotoxicity and mutagenicity of a new quantum dots (QDs) nanoplatform (QDsN), consisting of CdSe/ZnS core–shell QDs encapsulated by a natural fusogenic lipid (1,2-di-oleoyl-sn-glycero-3-phosphocholine (DOPC)) and functionalized by a nucleolipid N-[5′-(2′,3′-di-oleoyl) uridine]-N′,N′,N′-trimethylammoniumtosylate (DOTAU). This QDs nanoplatform may represent a new therapeutic tool for the diagnosis and treatment of human cancers. The genotoxic, mutagenic and clastogenic effects of QDsN were compared to those of cadmium chloride (CdCl2). Three assays were used: (1) the Salmonella/microsome assay with four tester strains, (2) the comet assay and (3) the micronucleus test on CHO cells. The contribution of simulated sunlight was studied in the three assays while oxidative events were only explored in the comet assay in aliquots pretreated with the antioxidant l-ergothioneine. We found that QDsN could enter CHO-K1 cells and accumulate in cytoplasmic vesicles. It was not mutagenic in the Salmonella/mutagenicity test whereas CdCl2 was weakly positive. In the dark, both the QDsN and CdCl2 similarly induced dose-dependent increases in single-strand breaks and micronuclei. Exposure to simulated sunlight significantly potentiated the genotoxic activities of both QDsN and CdCl2, but did not significantly increase micronucleus frequencies. l-Ergothioneine significantly reduced but did not completely suppress the DNA-damaging activity of QDsN and CdCl2. The present results clearly point to the genotoxic properties and the risk of long-term adverse effects of such a nanoplatform if used for human anticancer therapy and diagnosis in the future. 相似文献
40.
Narges Abdali Enrico Barth Amir Norouzy Robert Schulz Werner M. Nau Ulrich Kleinekath?fer Andreas Tauch Roland Benz 《PloS one》2013,8(10)
Corynebacterium jeikeium, a resident of human skin, is often associated with multidrug resistant nosocomial infections in immunodepressed patients. C. jeikeium K411 belongs to mycolic acid-containing actinomycetes, the mycolata and contains a channel-forming protein as judged from reconstitution experiments with artificial lipid bilayer experiments. The channel-forming protein was present in detergent treated cell walls and in extracts of whole cells using organic solvents. A gene coding for a 40 amino acid long polypeptide possibly responsible for the pore-forming activity was identified in the known genome of C. jeikeium by its similar chromosomal localization to known porH and porA genes of other Corynebacterium strains. The gene jk0268 was expressed in a porin deficient Corynebacterium glutamicum strain. For purification temporarily histidine-tailed or with a GST-tag at the N-terminus, the homogeneous protein caused channel-forming activity with an average conductance of 1.25 nS in 1M KCl identical to the channels formed by the detergent extracts. Zero-current membrane potential measurements of the voltage dependent channel implied selectivity for anions. This preference is according to single-channel analysis caused by some excess of cationic charges located in the channel lumen formed by oligomeric alpha-helical wheels. The channel has a suggested diameter of 1.4 nm as judged from the permeability of different sized hydrated anions using the Renkin correction factor. Surprisingly, the genome of C. jeikeium contained only one gene coding for a cell wall channel of the PorA/PorH type found in other Corynebacterium species. The possible evolutionary relationship between the heterooligomeric channels formed by certain Corynebacterium strains and the homooligomeric pore of C. jeikeium is discussed. 相似文献