全文获取类型
收费全文 | 71篇 |
免费 | 10篇 |
国内免费 | 1篇 |
出版年
2022年 | 2篇 |
2021年 | 3篇 |
2019年 | 1篇 |
2018年 | 2篇 |
2017年 | 2篇 |
2016年 | 3篇 |
2015年 | 4篇 |
2014年 | 9篇 |
2013年 | 3篇 |
2012年 | 2篇 |
2011年 | 1篇 |
2010年 | 3篇 |
2009年 | 3篇 |
2008年 | 1篇 |
2007年 | 3篇 |
2006年 | 2篇 |
2005年 | 3篇 |
2004年 | 4篇 |
2003年 | 2篇 |
2002年 | 1篇 |
2001年 | 2篇 |
2000年 | 2篇 |
1999年 | 2篇 |
1998年 | 3篇 |
1997年 | 3篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1986年 | 4篇 |
1985年 | 1篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1981年 | 1篇 |
排序方式: 共有82条查询结果,搜索用时 15 毫秒
11.
12.
K Michałek M Laszczyńska AK Ciechanowicz A Herosimczyk I Rotter M Oganowska 《Biotechnic & histochemistry》2014,89(5):342-347
Aquaporin 2 (AQP2) is a small, integral tetrameric plasma membrane protein that is expressed in mammalian kidneys. The specific constitution of this protein and its selective permeability to water means that AQP2 plays an important role in hypertonic urine production. Immunolocalization of AQP2 has been studied in humans, monkeys, sheep, dogs, rabbits, rats, mice and adult cattle. We analyzed the expression of AQP2 in kidneys of 7-month-old Polish-Friesian var. black and white male calves. AQP2 was localized in the principal cells of collecting ducts in medullary rays penetrating the renal cortex and in the collecting ducts of renal medulla. AQP2 was expressed most strongly in the apical plasma membrane, but expression was observed also in the intracellular vesicles and basolateral plasma membrane. Our study provides new information concerning the immunolocalization of AQP2 in calf kidneys. 相似文献
13.
Maia Mistral Oliver Buck Daniel C. Meier‐Behrmann David A. Burnett Terry E. Barnfield Andrew J. Scott Barbara J. Anderson J. Bastow Wilson 《植被学杂志》2000,11(6):911-916
Abstract. Four sites were sampled to determine spatial autocorrelation in vegetation at the community level. All were in western New Zealand, but on different substrates and of different physiognomy: a terrace forest, a floodplain forest, a mire and the middle of a logging road. In ‘dissimograms’the four communities all showed steady increases in dissimilarity with distance, but with shoulders in the curve for some sites, which could be related to plant morphology. Most of the increase in dissimilarity occurred over very short distances: less than 0.5 m in the forests, less than 1 m in the mire and less than 2 m in the road. Separate analyses of the woody and herbaceous guilds in the floodplain forest showed that herbaceous dissimilarities remained low at distances up to 20 m, probably because of clonal structure in some species. The mire showed low overall dissimilarity, which is attributed to the uniform substrate and the small species pool. Simulations showed that the approach is capable of indicating structure when it is present. Although the dissimogram was clearest when analysing a simulated grid of patches, other types of simulated patchiness showed dissimograms that were clearly distinguishable from those obtained from the vegetation studied. The almost continuous rise in dissimilarity with distance found in the four sites offers no support to the Hierarchy theory, fitting much more closely the alternative Continuum theory. 相似文献
14.
15.
The sequences of the 5' long-terminal repeat (LTR) and adjacent leader
regions of 27 full-length copia elements isolated from natural populations
of Drosophila melanogaster, D. simulans, and D. mauritiana are presented.
Phylogenetic analyses indicate that although D. melanogaster copia elements
are distinct from those of D. simulans and D. mauritiana, the elements of
these latter two species are not distinguishable from one another. LTRs and
adjacent 5' leader regions of elements isolated from D. simulans and D.
mauritiana are structurally similar to one another and carry substantial
deletional variation mapping to regions previously identified as being of
potential importance for copia expression.
相似文献
16.
Sphingosine 1‐phosphate and its carrier apolipoprotein M in human sepsis and in Escherichia coli sepsis in baboons
下载免费PDF全文
![点击此处可从《Journal of cellular and molecular medicine》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Cecilia Frej Adam Linder Kaisa E. Happonen Fletcher B. Taylor Florea Lupu Björn Dahlbäck 《Journal of cellular and molecular medicine》2016,20(6):1170-1181
Sphingosine 1‐phosphate (S1P) is an important regulator of vascular integrity and immune cell migration, carried in plasma by high‐density lipoprotein (HDL)‐associated apolipoprotein M (apoM) and by albumin. In sepsis, the protein and lipid composition of HDL changes dramatically. The aim of this study was to evaluate changes in S1P and its carrier protein apoM during sepsis. For this purpose, plasma samples from both human sepsis patients and from an experimental Escherichia coli sepsis model in baboons were used. In the human sepsis cohort, previously studied for apoM, plasma demonstrated disease‐severity correlated decreased S1P levels, the profile mimicking that of plasma apoM. In the baboons, a similar disease‐severity dependent decrease in plasma levels of S1P and apoM was observed. In the lethal E. coli baboon sepsis, S1P decreased already within 6–8 hrs, whereas the apoM decrease was seen later at 12–24 hrs. Gel filtration chromatography of plasma from severe human or baboon sepsis on Superose 6 demonstrated an almost complete loss of S1P and apoM in the HDL fractions. S1P plasma concentrations correlated with the platelet count but not with erythrocytes or white blood cells. The liver mRNA levels of apoM and apoA1 decreased strongly upon sepsis induction and after 12 hr both were almost completely lost. In conclusion, during septic challenge, the plasma levels of S1P drop to very low levels. Moreover, the liver synthesis of apoM decreases severely and the plasma levels of apoM are reduced. Possibly, the decrease in S1P contributes to the decreased endothelial barrier function observed in sepsis. 相似文献
17.
18.
A method is described using fast protein liquid chromatography (FPLC) for the monitoring of protein formation during fermentation. The procedure consists of centrifugation to recover the cells, sonication of the cells, centrifugation to remove cell debris, and analysis of supernatant on a column of Mono Q (a strong anion exchanger). Analysis of peak areas provides quantitative determination of product concentration. Maintenance and life of the Mono Q column is discussed. We find that FPLC is a convenient method for measuring products in cell homogenates because it gives rapid, highly resolved separations. 相似文献
19.
Rosalba Lepore Andriy Kryshtafovych Markus Alahuhta Harshul A. Veraszto Yannick J. Bomble Joshua C. Bufton Alex N. Bullock Cody Caba Hongnan Cao Owen R. Davies Ambroise Desfosses Matthew Dunne Krzysztof Fidelis Celia W. Goulding Manickam Gurusaran Irina Gutsche Christopher J. Harding Marcus D. Hartmann Christopher S. Hayes Andrzej Joachimiak Petr G. Leiman Peter Loppnau Andrew L. Lovering Vladimir V. Lunin Karolina Michalska Ignacio Mir-Sanchis AK Mitra John Moult George N. Phillips Jr Daniel M. Pinkas Phoebe A. Rice Yufeng Tong Maya Topf Jonathan D. Walton Torsten Schwede 《Proteins》2019,87(12):1037-1057
The functional and biological significance of selected CASP13 targets are described by the authors of the structures. The structural biologists discuss the most interesting structural features of the target proteins and assess whether these features were correctly reproduced in the predictions submitted to the CASP13 experiment. 相似文献