全文获取类型
收费全文 | 2553篇 |
免费 | 242篇 |
国内免费 | 32篇 |
出版年
2021年 | 25篇 |
2018年 | 29篇 |
2017年 | 35篇 |
2016年 | 39篇 |
2015年 | 56篇 |
2014年 | 79篇 |
2013年 | 92篇 |
2012年 | 87篇 |
2011年 | 116篇 |
2010年 | 71篇 |
2009年 | 79篇 |
2008年 | 97篇 |
2007年 | 79篇 |
2006年 | 87篇 |
2005年 | 88篇 |
2004年 | 81篇 |
2003年 | 87篇 |
2002年 | 81篇 |
2001年 | 91篇 |
2000年 | 80篇 |
1999年 | 52篇 |
1998年 | 34篇 |
1997年 | 31篇 |
1996年 | 33篇 |
1995年 | 38篇 |
1994年 | 20篇 |
1993年 | 22篇 |
1992年 | 55篇 |
1991年 | 44篇 |
1990年 | 51篇 |
1989年 | 56篇 |
1988年 | 51篇 |
1987年 | 45篇 |
1986年 | 28篇 |
1985年 | 39篇 |
1984年 | 36篇 |
1983年 | 31篇 |
1982年 | 23篇 |
1981年 | 31篇 |
1980年 | 29篇 |
1979年 | 21篇 |
1978年 | 24篇 |
1977年 | 34篇 |
1975年 | 27篇 |
1974年 | 35篇 |
1973年 | 32篇 |
1972年 | 24篇 |
1971年 | 34篇 |
1970年 | 30篇 |
1969年 | 26篇 |
排序方式: 共有2827条查询结果,搜索用时 359 毫秒
151.
Albert K. Hoang Duc Marc Modat Kelvin K. Leung M. Jorge Cardoso Josephine Barnes Timor Kadir Sébastien Ourselin for the Alzheimer’s Disease Neuroimaging Initiative 《PloS one》2013,8(8)
Multi-atlas segmentation has been widely used to segment various anatomical structures. The success of this technique partly relies on the selection of atlases that are best mapped to a new target image after registration. Recently, manifold learning has been proposed as a method for atlas selection. Each manifold learning technique seeks to optimize a unique objective function. Therefore, different techniques produce different embeddings even when applied to the same data set. Previous studies used a single technique in their method and gave no reason for the choice of the manifold learning technique employed nor the theoretical grounds for the choice of the manifold parameters. In this study, we compare side-by-side the results given by 3 manifold learning techniques (Isomap, Laplacian Eigenmaps and Locally Linear Embedding) on the same data set. We assess the ability of those 3 different techniques to select the best atlases to combine in the framework of multi-atlas segmentation. First, a leave-one-out experiment is used to optimize our method on a set of 110 manually segmented atlases of hippocampi and find the manifold learning technique and associated manifold parameters that give the best segmentation accuracy. Then, the optimal parameters are used to automatically segment 30 subjects from the Alzheimer’s Disease Neuroimaging Initiative (ADNI). For our dataset, the selection of atlases with Locally Linear Embedding gives the best results. Our findings show that selection of atlases with manifold learning leads to segmentation accuracy close to or significantly higher than the state-of-the-art method and that accuracy can be increased by fine tuning the manifold learning process. 相似文献
152.
A Modified RNA-Seq Approach for Whole Genome Sequencing of RNA Viruses from Faecal and Blood Samples
Elizabeth M. Batty T. H. Nicholas Wong Amy Trebes Karène Argoud Moustafa Attar David Buck Camilla L. C. Ip Tanya Golubchik Madeleine Cule Rory Bowden Charis Manganis Paul Klenerman Eleanor Barnes A. Sarah Walker David H. Wyllie Daniel J. Wilson Kate E. Dingle Tim E. A. Peto Derrick W. Crook Paolo Piazza 《PloS one》2013,8(6)
To date, very large scale sequencing of many clinically important RNA viruses has been complicated by their high population molecular variation, which creates challenges for polymerase chain reaction and sequencing primer design. Many RNA viruses are also difficult or currently not possible to culture, severely limiting the amount and purity of available starting material. Here, we describe a simple, novel, high-throughput approach to Norovirus and Hepatitis C virus whole genome sequence determination based on RNA shotgun sequencing (also known as RNA-Seq). We demonstrate the effectiveness of this method by sequencing three Norovirus samples from faeces and two Hepatitis C virus samples from blood, on an Illumina MiSeq benchtop sequencer. More than 97% of reference genomes were recovered. Compared with Sanger sequencing, our method had no nucleotide differences in 14,019 nucleotides (nt) for Noroviruses (from a total of 2 Norovirus genomes obtained with Sanger sequencing), and 8 variants in 9,542 nt for Hepatitis C virus (1 variant per 1,193 nt). The three Norovirus samples had 2, 3, and 2 distinct positions called as heterozygous, while the two Hepatitis C virus samples had 117 and 131 positions called as heterozygous. To confirm that our sample and library preparation could be scaled to true high-throughput, we prepared and sequenced an additional 77 Norovirus samples in a single batch on an Illumina HiSeq 2000 sequencer, recovering >90% of the reference genome in all but one sample. No discrepancies were observed across 118,757 nt compared between Sanger and our custom RNA-Seq method in 16 samples. By generating viral genomic sequences that are not biased by primer-specific amplification or enrichment, this method offers the prospect of large-scale, affordable studies of RNA viruses which could be adapted to routine diagnostic laboratory workflows in the near future, with the potential to directly characterize within-host viral diversity. 相似文献
153.
Co-administration of Selenium with Inorganic Mercury Alters the Disposition of Mercuric Ions in Rats
Orr Sarah E. George Hannah S. Barnes Mary C. Mathis Taylor N. Joshee Lucy Barkin Jennifer Kiefer Adam M. Seney Caryn S. Bridges Christy C. 《Biological trace element research》2020,195(1):187-195
Biological Trace Element Research - Mercury (Hg) is a common environmental toxicant to which humans are exposed regularly through occupational and dietary means. Although selenium supplementation... 相似文献
154.
155.
Paul S Marley Aboubacar Toure Jay Shebayan DA Aba AO Toure AG Diallo 《Archives Of Phytopathology And Plant Protection》2013,46(1):29-34
Fourteen elite sorghum lines were evaluated for their resistance to Striga hermonthica at three locations in Nigeria and Mali. Results showed that many of the lines especially MALISOR 84-1, SAMSORG 41, 97-SB-F5DT-64 (Keninkédié) and the check SRN 39 remained resistant to Striga in all locations with low emerged Striga counts, while SAMSORG 14 had the highest Striga infestation in all locations. Considerable variation in reaction to Striga infestation was observed on Séguètana, 97-SB-F5DT-63 (Wasa), 97-SB-F5DT-65, CMDT 38, CMDT 39 and CMDT 45 which were susceptible to Striga at Samaru, Nigeria but were resistant to Striga at both locations in Mali. Based on low Striga resistance and high grain yield, lines MALISOR 84-1, SAMSORG 41, 97-SB-F5DT-64, 97-SB-F5DT-65, CMDT 39 and SAMSORT 14 have been nominated for wider evaluation across more West African countries. 相似文献
156.
157.
Stephen P. Barnes Samuel D. Bradbrook Barry A. Cragg Julian R. Marchesi Andrew J. Weightman John C. Fry 《Geomicrobiology journal》2013,30(2):67-83
Bacterial populations exist at great depths in marine sediments, but little is known about the type and characteristics of organisms in this unique bacterial environment. Cascadia Margin sediments from the Pacific Ocean have deep bacterial activity and bacterial populations, which are stimulated around a gas hydrate zone (215–225 m below sea floor [mbsf]). Bacterial sulfate reduction is the dominant anaerobic process within these sediments, and the depth distribution of sulfate‐reducing activity corresponds with distributions of viable sulfate‐reducing bacteria (SRB). Anaerobically stored sediments from this site were used to isolate sulfate‐reducing bacteria using a temperature‐gradient system, elevated pressure and temperatures, different media, and a range of growth substrates. A variety of enrichments on lactate were obtained from 0.5 and 222 mbsf, with surprisingly more rapid growth from the deeper sediments. The temperature range of enrichments producing strong growth from 222 mbsf was markedly wider than those from the near surface sediment (15–45°C and 9–19°C, respectively). This presumably reflects a temperature increase in deeper sediments. Only a few of these enrichments were successfully isolated due to very slow or no growth on subculture, despite the use of a wide range of different media and growth conditions. Psychrophilic and mesophilic sulfate‐reducing isolates were obtained from 0.5 m depth. As the minimum growth temperature of the mesophile (probably a Desulfotomaculum sp.) was above the in situ temperature of 3°C, it must have been present in the sediment as spores. A larger number of isolates (23) was obtained from 222 mbsf, and these barophilic SRB were closely related (based on 16S rRNA gene analysis), but not identical to, Desulfovibrio profundus, recently isolated from deep sediments from the Japan Sea. Bacteria related to D. profundus may be widespread in deep marine sediments. 相似文献
158.
Meirav Meiri Adrian M. Lister Thomas F. G. Higham John R. Stewart Lawrence G. Straus Henriette Obermaier Manuel R. González Morales Ana B. Marín‐Arroyo Ian Barnes 《Molecular ecology》2013,22(18):4711-4722
The Pleistocene was an epoch of extreme climatic and environmental changes. How individual species responded to the repeated cycles of warm and cold stages is a major topic of debate. For the European fauna and flora, an expansion–contraction model has been suggested, whereby temperate species were restricted to southern refugia during glacial times and expanded northwards during interglacials, including the present interglacial (Holocene). Here, we test this model on the red deer (Cervus elaphus) a large and highly mobile herbivore, using both modern and ancient mitochondrial DNA from the entire European range of the species over the last c. 40 000 years. Our results indicate that this species was sensitive to the effects of climate change. Prior to the Last Glacial Maximum (LGM) haplogroups restricted today to South‐East Europe and Western Asia reached as far west as the UK. During the LGM, red deer was mainly restricted to southern refugia, in Iberia, the Balkans and possibly in Italy and South‐Western Asia. At the end of the LGM, red deer expanded from the Iberian refugium, to Central and Northern Europe, including the UK, Belgium, Scandinavia, Germany, Poland and Belarus. Ancient DNA data cannot rule out refugial survival of red deer in North‐West Europe through the LGM. Had such deer survived, though, they were replaced by deer migrating from Iberia at the end of the glacial. The Balkans served as a separate LGM refugium and were probably connected to Western Asia with genetic exchange between the two areas. 相似文献
159.
Eleftheria Palkopoulou Love Dalén Adrian M. Lister Sergey Vartanyan Mikhail Sablin Andrei Sher Veronica Nystr?m Edmark Mikael D. Brandstr?m Mietje Germonpré Ian Barnes Jessica A. Thomas 《Proceedings. Biological sciences / The Royal Society》2013,280(1770)
Ancient DNA analyses have provided enhanced resolution of population histories in many Pleistocene taxa. However, most studies are spatially restricted, making inference of species-level biogeographic histories difficult. Here, we analyse mitochondrial DNA (mtDNA) variation in the woolly mammoth from across its Holarctic range to reconstruct its history over the last 200 thousand years (kyr). We identify a previously undocumented major mtDNA lineage in Europe, which was replaced by another major mtDNA lineage 32–34 kyr before present (BP). Coalescent simulations provide support for demographic expansions at approximately 121 kyr BP, suggesting that the previous interglacial was an important driver for demography and intraspecific genetic divergence. Furthermore, our results suggest an expansion into Eurasia from America around 66 kyr BP, coinciding with the first exposure of the Bering Land Bridge during the Late Pleistocene. Bayesian inference indicates Late Pleistocene demographic stability until 20–15 kyr BP, when a severe population size decline occurred. 相似文献
160.
ABSTRACT The vocal repertoire of Amazona amazonica during its breeding season has been recorded from wild individuals in Santa Bárbara do Pará, Pará State, Brazil. At individual nests, we continuously recorded vocalizations and behaviour for four hours in the early morning and three hours in the late afternoon, three times a week throughout the breeding season. We identified nine vocalizations that we classified in three behavioural categories: (1) Flight call—emitted when parrots arrive in the nest area; (2) Perched contact calls—two different vocalizations, one of them related to feeding, were emitted when the pair was perched in the nest area and interacted socially between themselves or with other individuals; (3) Aggressive calls—emitted when birds were in a dangerous situation, i.e. alarm (three types of calls), agonistic contact and distress calls (two types of call). The Orange-winged Parrot is a highly social species and the complexity of its social interactions is reflected in the diversity of its vocal repertoire. 相似文献