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41.
1. Acridine Orange inhibits growth of Escherichia coli K12 when incubated at pH 7.9, but not at pH 7.4.2. At a non-permissive temperature for DNA polymerase I, Acridine Orange inhibits growth of a temperature-sensitive strain and also increases the rate of elimination of the F'-Lac plasmid. 3. DNA isolated from cells treated with Acridine Orange under conditions that inhibit growth contains material of low molecular weight, which is absent from DNA isolated from cells treated under conditions in which growth is not impaired. 4. Cells incubated with Acridine Orange at both pH 7.4 and 7.9 suffer degradation of DNA, as shown by loss of labelled DNA from the acid-insoluble fraction, which is not observed with untreated cells at either pH. 5. The results suggest that elimination of the F'-Lac plasmid by Acridine Orange requires inactivation of repair processes. 相似文献
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In vivo synthesised protein with norleucine occupying one half of the normal methionine loci was prepared using a methionine auxotroph of Escherichia coli K12. The extent of charging of the analogue onto both tRNAmet species and subsequent incorporation into soluble protein was monitored with a double-labelling system comprising [G-3H]norleucine and [35S]methionine. Further experiments established that norleucine can be formylated in vivo once charged onto the initiator tRNAfmet. An N-terminal analysis of the crude soluble protein revealed that formylnorleucyl-tRNAfmet can initiate protein synthesis and that the formyl group is then removed from the nascent polypeptide. We were also led to conclude that the N-terminal methionine-amino peptidase does not recognise the analogue in this position. Slow growth rates on the methionine analogue have been partly attributed to limiting levels of charged tRNAmmet, resulting in turn from the inefficiency of norleucine charging by methionyl-tRNA synthetase. Finally no evidence has been found for the production of aberrant protein as a result of norleucine incorporation, implying that limited growth on the analogue is due to its inability to replace methionine as the precursor of S-adenosyl methionine. 相似文献
45.
Coccidial oocysts morphologically consistent with Eimeria ursini Supperer 1957, and E. tasmaniae Supperer 1957 were recovered from the feces of wild and captive hairy-nosed wombats (Lasiorhinus latifrons) in Australia. Eimeria arundeli so. n. was recovered from the feces of wild and captive common wombats (Vombatus ursinus). Eimeria arundeli oocysts are ellipsoidal to slightly ovoid 60.2--67.2 (63.7) X 40.6--47.6 (43.4); micropyle 3 in diameter usually visible; with oocyst wall granular, dark brown and occasionally opaque, 4--7 thick; inner oocyst wall clear, about 1.5 thick; small oocyst residuum present, four sporocysts ovoid 22.4--29.4 (25.8) X 12.6--15.4 (14.1) with protuberant Stieda body; opposite end of sporocyst also often slighly pointed; large granular sporocyst residuum obscuring sporozoites. Gametocytes of E. arundeli sp. n. and of an organism which is consistent with E. tasmaniae, are described developing in the lamina propria of villi in the small intestine. The stages in the hairy-nosed wombat are those described as Ileocystis wombati Gilruth and Bull 1912. It is suggested that the identification of the host of Supperer's E. ursini and E. tasmaniae as V. ursinus was in error and that the allopatric L. latifrons is the natural host. Eimeria tasmaniae Supperer 1957 is suppressed and E. wombati (Gilruth and Bull, 1912) comb. nov. is proposed and redescribed. No schizonts were identified among the endogenous stages, consistent with observations in the literature on other coccidia with similar gametocyte and oocyst structure. 相似文献
46.
Winona C. Barker Lynne K. Ketcham Margaret O. Dayhoff 《Journal of molecular evolution》1978,10(4):265-281
Summary We have implemented a routine procedure for screening protein sequences for evidence of intragenic duplications. We tested 163 protein sequences representing 116 superfamilies of unrelated proteins. Twenty superfamilies contain proteins with internal gene duplications. The intragenic duplications detected can be divided into two major types. (1) One or more duplications of all or part of a gene produce a protein with two or several detectable regions of sequence homology. Sequences from 18 superfamilies contained this type of duplication. (2) Repeated reduplication of a small DNA segment can produce a protein that is repetitive over most of its length. Three superfamilies contain such repetitive sequences. We also investigated the limits of detection of ancient duplications using sequences derived by random mutation of a model sequence consisting of ten 10-residue repeats. The original repetitive nature of the sequence was usually detected after 250 point mutations even though the ancestral segment could not be accurately reconstructed. 相似文献
47.
The radiological prevalence of Paget''s disease was studied in 14 towns. Routine radiographs showed that the disease was present in 5.4% of people aged 55 years and over. The disease was more prevalent in men than in women at all ages, and the prevalence increased with age. The three Lancashire towns studied (Preston, Bolton, and Blackburn) had higher rates than elsewhere. This probably reflects a real geographical variation in the prevalence of Paget''s disease in England and Wales. 相似文献
48.
Using UDP-d-galactose : 2-acetamido-2-deoxy-d-glucose 4-β-d-galactosyltransferase (EC 2.4.1.22), several methyl β-lactosides have been prepared with 2H- and/or 13C-enrichment at specific sites to facilitate study by 13C (75 MHz) and 1H (600 MHz) n.m.r. spectroscopy. 13C-Chemical shift assignments were verified and the 1H-spectrum of β-lactoside was fully assigned. Sites of enrichment were selected to permit all of the potential three-bond C-C and C-H couplings through the glycosidic bond to be obtained. Replacement of H-3 of the d-glucose residue of methyl β-lactoside with 2H allowed resolution of C-1–H-4′ coupling in the 600-MHz 1H-spectrum. Single or multiple 13C-enrichment at C-1, C-2, C-3, C-1′, C-3′, and/or C-4′ in the disaccharide allowed observation of intra- and inter-residue couplings. 13C-Spin-lattice relaxation-times (T1) are interpreted in terms of molecular motion in solution. The data suggest that methyl β-lactoside has an extended conformation with little rotation about the glycosidic bond. Inter-residue couplings are best explained by tortion angles of φ ~ 40° and ψ ~ 15°, indicating that the conformations of β-lactoside in solution and in the crystal are similar. 相似文献
49.
Methionyl-tRNA synthetase from Escherichia coli. Primary structure of the active crystallised tryptic fragment 总被引:14,自引:0,他引:14
A 3300-base segment of Escherichia coli chromosomal DNA, cloned into pBR322, will complement a methionine auxotroph in which the lesion is a defective methionyl-tRNA synthetase with a much reduced affinity for methionine. Crude extracts of these transformants contain elevated levels of a protein which has a subunit molecular weight of 66 000, methionyl-tRNA synthetase aminoacylation activity in vitro and which cross-reacts with anti-(methionyl-tRNA synthetase) antibodies. This polypeptide is very slightly larger than the well-characterised and crystallised tryptic fragment of methionyl-tRNA synthetase. A DNA sequence of 1750 residues at one end of the cloned insert codes for a non-terminated open reading frame in which we can locate a large number of methionyl-tRNA synthetase tryptic and chymotryptic peptides. We have also sequenced 300 nucleotides upstream of this coding segment where we find a large invert repeat in the putative methionyl-tRNA synthetase promoter region. 相似文献
50.
P A Barker F D Miller T H Large R A Murphy 《The Journal of biological chemistry》1991,266(28):19113-19119
These studies were initiated to determine whether the soluble, truncated form of the nerve growth factor (NGF) receptor arises from post-translational processing of the intact, membrane-bound receptor or from an alternatively spliced mRNA. Pulse-chase analysis of cultured primary rat Schwann cells coupled with immunoprecipitations using antibodies to the intracellular and extracellular domains of the receptor were used to monitor receptor production. Three forms of the NGF receptor (80, 83, and 85 kDa) displaying a precursor product relationship were detected over the 2-h chase period; only the 85-kDa species was detected on the cell surface. Truncated receptors (50 and 52 kDa) were detected in conditioned media 5 h after cell labeling but were never observed intracellularly. Polymerase chain reaction and RNase protection analyses of NGF receptor mRNA targeted toward the coding region for the transmembrane domain detected no splice variants that could generate truncated receptor, and media conditioned by fibroblasts transfected with rat receptor cDNA, in which splicing cannot occur, nonetheless contained the truncated receptor protein. Taken together, these results suggest that the truncated NGF receptor does not arise as a distinct translation product but rather from a post-translational modification of the intact, surface-bound form of the protein. 相似文献