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81.
82.
K L Lucas K W Barbour P R Housley E A Thompson 《Molecular endocrinology (Baltimore, Md.)》1988,2(3):291-299
Three phenotypically distinct isolates from lymphosarcoma P1798 have been compared with respect to properties of the glucocorticoid receptor. Wild type P1798 cells express functional receptors and glucocorticoid treatment of such cells causes cytolysis in vivo. Wild type cells do not undergo cytolysis in culture. Rather, such cells exhibit reversible inhibition of proliferation in the presence of dexamethasone. Two variant populations were selected from this background. One was selected for the ability to form tumors in mice receiving pharmacological doses of glucocorticoids. Cells from such tumors are resistant to the cytolytic effects of glucocorticoids in vivo, but are sensitive to the antiproliferative effects of the hormone in culture. Variants were also selected based upon their ability to proliferate in the presence of dexamethasone in culture. These variants were resistant to glucocorticoid-mediated cytolysis in vivo. Wild type P1798 cells express approximately 20,000 high affinity dexamethasone-binding sites per cell. Dexamethasone-mesylate labeling and immunoblotting experiments indicate that hormone binding is due to a polypeptide of Mr 90-100 K. This polypeptide is encoded in an mRNA species that resolved as a single entity of approximately 7000 nucleotides. Variants selected for resistance to cytolysis in vivo are indistinguishable in any of these respects from wild type cells. The receptors are fully functional, as evidenced by their ability to precipitate growth arrest of dexamethasone-treated cultures. Variants selected for resistance in culture harbor a receptor mutation. They express fewer than 500 dexamethasone-binding sites per cell. Such variants contain neither detectable dexamethasone-mesylate-binding protein nor any protein that is recognized by a receptor antibody.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
83.
A. A. Da Rosa Pinto D. Y. Barbour Valerie Gargett Dora G. Webb A. C. Kemp G. L. Maclean 《Ostrich》2013,84(1):79-80
2010, Lynx Edicions, Barcelona, Spain 880 pages, 60 colour plates, hardcover ISBN-13: 978–84-96553–68-2. Price €212 相似文献
84.
H. G. Barbour 《CMAJ》1921,11(12):944-945
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The ultrastructure of the head end and surface structure of the cuticle of Syphacia petrusewiczi, S. nigeriana, S. frederici and S. stroma was studied. These species may be easily separated on the basis of the differences in their morphology: S. frederici possesses longitudinal septa on the body surface, a row of denticles on each of the three main teeth, and cervical alae; S. nigeriana has longitudinal septa and denticles but lack cervical alae; S. petrusewiczi has longitudinal septa and cervical alae but lacks denticles; S. stroma lacks these three characters. 相似文献
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